Structured Review

TaKaRa uracil dna glycosylase
Uracil Dna Glycosylase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/uracil dna glycosylase/product/TaKaRa
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
uracil dna glycosylase - by Bioz Stars, 2020-07
92/100 stars

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Polymerase Chain Reaction:

Article Title: Development and validation of a method for human papillomavirus genotyping based on molecular beacon probes
Article Snippet: .. Both reaction mixture contains 1× PCR Buffer (Takara, Japan), 2 mM MgCl2 (Promega, USA), 12.5 nM dU plus deoxynucleotide triphosphates (dNTPs, Takara), 37.5 nM limiting primer MY11 ( 5′-GCMCAGGGWCATAAYAATGG-3′ ), 500 nM excess primer GP6+ ( 5′-GAAAAATAAACTGTAAATCATATTC-3′ ), two units Hot-Start Taq polymerase (Takara), and one unit Uracil DNA Glycosylase (UNG, Takara). ..

Article Title: Development and validation of a method for human papillomavirus genotyping based on molecular beacon probes
Article Snippet: .. Both reaction mixture contains 1× PCR Buffer (Takara, Japan), 2 mM MgCl2 (Promega, USA), 12.5 nM dU plus deoxynucleotide triphosphates (dNTPs, Takara), 37.5 nM limiting primer MY11 ( 5′-GCMCAGGGWCATAAYAATGG-3′ ), 500 nM excess primer GP6+ ( 5′-GAAAAATAAACTGTAAATCATATTC-3′ ), two units Hot-Start Taq polymerase (Takara), and one unit Uracil DNA Glycosylase (UNG, Takara). ..

Article Title: Detection of Candida species in pregnant Chinese women with a molecular beacon method
Article Snippet: .. To identify each of the five Candida spp., the species-specific PCR reaction mixtures (40 µl) were set up as follows: 5 ng template DNA, 0.2 mM dNTPs, 0.6 µM primers mix, 0.1 µM probe, 2.5 U Taq polymerase (TaKaRa Bio, Inc) and 0.2 U uracil-N-glycosylase (TaKaRa Bio, Inc). .. Forty amplification cycles were performed (20 s at 94 °C and 45 s at 55 °C) for each reaction tube.

Article Title: Detection of Candida species in pregnant Chinese women with a molecular beacon method
Article Snippet: .. To identify each of the five Candida spp., the species-specific PCR reaction mixtures (40 µl) were set up as follows: 5 ng template DNA, 0.2 mM dNTPs, 0.6 µM primers mix, 0.1 µM probe, 2.5 U Taq polymerase (TaKaRa Bio, Inc) and 0.2 U uracil-N-glycosylase (TaKaRa Bio, Inc). .. Forty amplification cycles were performed (20 s at 94 °C and 45 s at 55 °C) for each reaction tube.

Article Title: High aspect ratio induced spontaneous generation of monodisperse picolitre droplets for digital PCR
Article Snippet: .. The PCR mixture in a total volume of 20 μ l contained 5 μ l of 2× Light Cycler 480 Probe Master, 0.2U of Uracil DNA Glycosylase (Takara, Japan), 400 nM each of forward and reverse primers, 250 nM MGB probe, and the gDNA sample of various concentrations. .. All reaction components, including the PCR master mix, primers, probes, and DNA template, were premixed off-chip before sampling.

Sequencing:

Article Title: Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
Article Snippet: .. Sequence-independent cloning is largely based on homologous recombination and includes methods such as ligase-free or ligation-independent cloning (LIC) , LIC with Uracil DNA glycosylase (UDG or USER cloning) , , MAGIC , SLIC , In-Fusion (Clontech) , and PIPE . .. Although these methods all have their own special characteristics and advantages, new developments especially the emergence of synthetic biology have put ever increasing demand for more accurate, efficient, convenient and economical cloning technologies for purposes such as creating complex combinatorial synthetic gene libraries, gene circuits and metabolic pathways.

Clone Assay:

Article Title: Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
Article Snippet: .. Sequence-independent cloning is largely based on homologous recombination and includes methods such as ligase-free or ligation-independent cloning (LIC) , LIC with Uracil DNA glycosylase (UDG or USER cloning) , , MAGIC , SLIC , In-Fusion (Clontech) , and PIPE . .. Although these methods all have their own special characteristics and advantages, new developments especially the emergence of synthetic biology have put ever increasing demand for more accurate, efficient, convenient and economical cloning technologies for purposes such as creating complex combinatorial synthetic gene libraries, gene circuits and metabolic pathways.

Homologous Recombination:

Article Title: Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
Article Snippet: .. Sequence-independent cloning is largely based on homologous recombination and includes methods such as ligase-free or ligation-independent cloning (LIC) , LIC with Uracil DNA glycosylase (UDG or USER cloning) , , MAGIC , SLIC , In-Fusion (Clontech) , and PIPE . .. Although these methods all have their own special characteristics and advantages, new developments especially the emergence of synthetic biology have put ever increasing demand for more accurate, efficient, convenient and economical cloning technologies for purposes such as creating complex combinatorial synthetic gene libraries, gene circuits and metabolic pathways.

Ligation:

Article Title: Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
Article Snippet: .. Sequence-independent cloning is largely based on homologous recombination and includes methods such as ligase-free or ligation-independent cloning (LIC) , LIC with Uracil DNA glycosylase (UDG or USER cloning) , , MAGIC , SLIC , In-Fusion (Clontech) , and PIPE . .. Although these methods all have their own special characteristics and advantages, new developments especially the emergence of synthetic biology have put ever increasing demand for more accurate, efficient, convenient and economical cloning technologies for purposes such as creating complex combinatorial synthetic gene libraries, gene circuits and metabolic pathways.

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    TaKaRa uracil n glycosylase
    Uracil N Glycosylase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uracil n glycosylase/product/TaKaRa
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    uracil n glycosylase - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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    TaKaRa anti contamination enzyme udg
    Anti Contamination Enzyme Udg, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti contamination enzyme udg/product/TaKaRa
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti contamination enzyme udg - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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