Structured Review

TaKaRa uracil dna glycosylase
Uracil Dna Glycosylase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/uracil dna glycosylase/product/TaKaRa
Average 92 stars, based on 5 article reviews
Price from $9.99 to $1999.99
uracil dna glycosylase - by Bioz Stars, 2019-10
92/100 stars

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Related Articles

Electrophoresis:

Article Title: Sequence-specific electron injection into DNA from an intermolecular electron donor
Article Snippet: The photoirradiated DNAs containing uracil were cleaved via treatment with two units of Uracil DNA Glycosylase, UNG (Takara) for 1 h at 37°C, followed by heat treatment at 95°C for 10 min with 0.1 M NaOH. .. The photoirradiated DNAs containing uracil were cleaved via treatment with two units of Uracil DNA Glycosylase, UNG (Takara) for 1 h at 37°C, followed by heat treatment at 95°C for 10 min with 0.1 M NaOH.

Polymerase Chain Reaction:

Article Title: High aspect ratio induced spontaneous generation of monodisperse picolitre droplets for digital PCR
Article Snippet: A HEX dye-labelled MGB hydrolysis probe (5′ HEX-CCAAGCGACGGTCCTC-MGB 3′, Invitrogen) was used to trace all the PCR products. .. The PCR mixture in a total volume of 20 μ l contained 5 μ l of 2× Light Cycler 480 Probe Master, 0.2U of Uracil DNA Glycosylase (Takara, Japan), 400 nM each of forward and reverse primers, 250 nM MGB probe, and the gDNA sample of various concentrations. .. All reaction components, including the PCR master mix, primers, probes, and DNA template, were premixed off-chip before sampling.

Article Title: Detection of Candida species in pregnant Chinese women with a molecular beacon method
Article Snippet: The molecular beacon assay was carried out with the Applied Biosystems 7500 Real-Time PCR (Thermo Fisher). .. To identify each of the five Candida spp., the species-specific PCR reaction mixtures (40 µl) were set up as follows: 5 ng template DNA, 0.2 mM dNTPs, 0.6 µM primers mix, 0.1 µM probe, 2.5 U Taq polymerase (TaKaRa Bio, Inc) and 0.2 U uracil-N-glycosylase (TaKaRa Bio, Inc). .. Forty amplification cycles were performed (20 s at 94 °C and 45 s at 55 °C) for each reaction tube.

Article Title: Development and validation of a method for human papillomavirus genotyping based on molecular beacon probes
Article Snippet: LATE-PCR was performed in a volume of 40 μl with 5 μl of extracted DNA as template. .. Both reaction mixture contains 1× PCR Buffer (Takara, Japan), 2 mM MgCl2 (Promega, USA), 12.5 nM dU plus deoxynucleotide triphosphates (dNTPs, Takara), 37.5 nM limiting primer MY11 ( 5′-GCMCAGGGWCATAAYAATGG-3′ ), 500 nM excess primer GP6+ ( 5′-GAAAAATAAACTGTAAATCATATTC-3′ ), two units Hot-Start Taq polymerase (Takara), and one unit Uracil DNA Glycosylase (UNG, Takara). .. Probes in reaction A were P6(25 nM), P58 (15 nM ), P68(15 nM), and P59(20 nM), and for reaction B there were P51(25 nM), P66(25 nM), P73(15 nM), P83(15 nM), P45(20 nM) and P53(20 nM).

Article Title: Development and validation of a method for human papillomavirus genotyping based on molecular beacon probes
Article Snippet: LATE-PCR was performed in a volume of 40 μl with 5 μl of extracted DNA as template. .. Both reaction mixture contains 1× PCR Buffer (Takara, Japan), 2 mM MgCl2 (Promega, USA), 12.5 nM dU plus deoxynucleotide triphosphates (dNTPs, Takara), 37.5 nM limiting primer MY11 ( 5′-GCMCAGGGWCATAAYAATGG-3′ ), 500 nM excess primer GP6+ ( 5′-GAAAAATAAACTGTAAATCATATTC-3′ ), two units Hot-Start Taq polymerase (Takara), and one unit Uracil DNA Glycosylase (UNG, Takara). .. Probes in reaction A were P6(25 nM), P58 (15 nM ), P68(15 nM), and P59(20 nM), and for reaction B there were P51(25 nM), P66(25 nM), P73(15 nM), P83(15 nM), P45(20 nM) and P53(20 nM).

Article Title: Detection of Candida species in pregnant Chinese women with a molecular beacon method
Article Snippet: The molecular beacon assay was carried out with the Applied Biosystems 7500 Real-Time PCR (Thermo Fisher). .. To identify each of the five Candida spp., the species-specific PCR reaction mixtures (40 µl) were set up as follows: 5 ng template DNA, 0.2 mM dNTPs, 0.6 µM primers mix, 0.1 µM probe, 2.5 U Taq polymerase (TaKaRa Bio, Inc) and 0.2 U uracil-N-glycosylase (TaKaRa Bio, Inc). .. Forty amplification cycles were performed (20 s at 94 °C and 45 s at 55 °C) for each reaction tube.

Real-time Polymerase Chain Reaction:

Article Title: Detection of Candida species in pregnant Chinese women with a molecular beacon method
Article Snippet: The molecular beacon assay was carried out with the Applied Biosystems 7500 Real-Time PCR (Thermo Fisher). .. To identify each of the five Candida spp., the species-specific PCR reaction mixtures (40 µl) were set up as follows: 5 ng template DNA, 0.2 mM dNTPs, 0.6 µM primers mix, 0.1 µM probe, 2.5 U Taq polymerase (TaKaRa Bio, Inc) and 0.2 U uracil-N-glycosylase (TaKaRa Bio, Inc).

Article Title: Detection of Candida species in pregnant Chinese women with a molecular beacon method
Article Snippet: The molecular beacon assay was carried out with the Applied Biosystems 7500 Real-Time PCR (Thermo Fisher). .. To identify each of the five Candida spp., the species-specific PCR reaction mixtures (40 µl) were set up as follows: 5 ng template DNA, 0.2 mM dNTPs, 0.6 µM primers mix, 0.1 µM probe, 2.5 U Taq polymerase (TaKaRa Bio, Inc) and 0.2 U uracil-N-glycosylase (TaKaRa Bio, Inc).

Concentration Assay:

Article Title: Detection of Candida species in pregnant Chinese women with a molecular beacon method
Article Snippet: Extracted DNA was stored at −20 °C until use, with the concentration being measured with NanoDrop (Thermo Scientific) at 280/260 nm. .. To identify each of the five Candida spp., the species-specific PCR reaction mixtures (40 µl) were set up as follows: 5 ng template DNA, 0.2 mM dNTPs, 0.6 µM primers mix, 0.1 µM probe, 2.5 U Taq polymerase (TaKaRa Bio, Inc) and 0.2 U uracil-N-glycosylase (TaKaRa Bio, Inc).

Article Title: Detection of Candida species in pregnant Chinese women with a molecular beacon method
Article Snippet: Extracted DNA was stored at −20 °C until use, with the concentration being measured with NanoDrop (Thermo Scientific) at 280/260 nm. .. To identify each of the five Candida spp., the species-specific PCR reaction mixtures (40 µl) were set up as follows: 5 ng template DNA, 0.2 mM dNTPs, 0.6 µM primers mix, 0.1 µM probe, 2.5 U Taq polymerase (TaKaRa Bio, Inc) and 0.2 U uracil-N-glycosylase (TaKaRa Bio, Inc).

Polyacrylamide Gel Electrophoresis:

Article Title: Sequence-specific electron injection into DNA from an intermolecular electron donor
Article Snippet: Paragraph title: Polyacrylamide gel electrophoresis analysis ... The photoirradiated DNAs containing uracil were cleaved via treatment with two units of Uracil DNA Glycosylase, UNG (Takara) for 1 h at 37°C, followed by heat treatment at 95°C for 10 min with 0.1 M NaOH.

Sequencing:

Article Title: High aspect ratio induced spontaneous generation of monodisperse picolitre droplets for digital PCR
Article Snippet: Reverse primer: 5′-CACCTCCTTACTTTGCCT-3′) was used to amplify a 214 bp product of EGFR exon 21 sequence. .. The PCR mixture in a total volume of 20 μ l contained 5 μ l of 2× Light Cycler 480 Probe Master, 0.2U of Uracil DNA Glycosylase (Takara, Japan), 400 nM each of forward and reverse primers, 250 nM MGB probe, and the gDNA sample of various concentrations.

Software:

Article Title: High aspect ratio induced spontaneous generation of monodisperse picolitre droplets for digital PCR
Article Snippet: The primers and probes were designed with the Primer premier 5 software according to the EGFR gDNA sequence. .. The PCR mixture in a total volume of 20 μ l contained 5 μ l of 2× Light Cycler 480 Probe Master, 0.2U of Uracil DNA Glycosylase (Takara, Japan), 400 nM each of forward and reverse primers, 250 nM MGB probe, and the gDNA sample of various concentrations.

Article Title: Detection of Candida species in pregnant Chinese women with a molecular beacon method
Article Snippet: Based on the distinct sequences of the internal transcribed spacer 2 (ITS2) of Candida spp., the primers and hybridization probes were designed using Primer Premier 5.0 software (PREMIER Biosoft) (Table S1, available in the online version of this article). .. To identify each of the five Candida spp., the species-specific PCR reaction mixtures (40 µl) were set up as follows: 5 ng template DNA, 0.2 mM dNTPs, 0.6 µM primers mix, 0.1 µM probe, 2.5 U Taq polymerase (TaKaRa Bio, Inc) and 0.2 U uracil-N-glycosylase (TaKaRa Bio, Inc).

Article Title: Detection of Candida species in pregnant Chinese women with a molecular beacon method
Article Snippet: Based on the distinct sequences of the internal transcribed spacer 2 (ITS2) of Candida spp., the primers and hybridization probes were designed using Primer Premier 5.0 software (PREMIER Biosoft) (Table S1, available in the online version of this article). .. To identify each of the five Candida spp., the species-specific PCR reaction mixtures (40 µl) were set up as follows: 5 ng template DNA, 0.2 mM dNTPs, 0.6 µM primers mix, 0.1 µM probe, 2.5 U Taq polymerase (TaKaRa Bio, Inc) and 0.2 U uracil-N-glycosylase (TaKaRa Bio, Inc).

Hybridization:

Article Title: Detection of Candida species in pregnant Chinese women with a molecular beacon method
Article Snippet: Based on the distinct sequences of the internal transcribed spacer 2 (ITS2) of Candida spp., the primers and hybridization probes were designed using Primer Premier 5.0 software (PREMIER Biosoft) (Table S1, available in the online version of this article). .. To identify each of the five Candida spp., the species-specific PCR reaction mixtures (40 µl) were set up as follows: 5 ng template DNA, 0.2 mM dNTPs, 0.6 µM primers mix, 0.1 µM probe, 2.5 U Taq polymerase (TaKaRa Bio, Inc) and 0.2 U uracil-N-glycosylase (TaKaRa Bio, Inc).

Article Title: Detection of Candida species in pregnant Chinese women with a molecular beacon method
Article Snippet: Based on the distinct sequences of the internal transcribed spacer 2 (ITS2) of Candida spp., the primers and hybridization probes were designed using Primer Premier 5.0 software (PREMIER Biosoft) (Table S1, available in the online version of this article). .. To identify each of the five Candida spp., the species-specific PCR reaction mixtures (40 µl) were set up as follows: 5 ng template DNA, 0.2 mM dNTPs, 0.6 µM primers mix, 0.1 µM probe, 2.5 U Taq polymerase (TaKaRa Bio, Inc) and 0.2 U uracil-N-glycosylase (TaKaRa Bio, Inc).

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    TaKaRa uracil dna glycosylase
    Uracil Dna Glycosylase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uracil dna glycosylase/product/TaKaRa
    Average 79 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    uracil dna glycosylase - by Bioz Stars, 2019-10
    79/100 stars
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