ultratm ii directional rna library prep kit  (New England Biolabs)


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    Name:
    NEBNext Ultra II RNA Library Prep Kit for Illumina
    Description:
    NEBNext Ultra II RNA Library Prep Kit for Illumina 96 rxns
    Catalog Number:
    e7770l
    Price:
    3025
    Size:
    96 rxns
    Category:
    mRNA Template Preparation for PCR
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    New England Biolabs ultratm ii directional rna library prep kit
    NEBNext Ultra II RNA Library Prep Kit for Illumina
    NEBNext Ultra II RNA Library Prep Kit for Illumina 96 rxns
    https://www.bioz.com/result/ultratm ii directional rna library prep kit/product/New England Biolabs
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ultratm ii directional rna library prep kit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Comparative transcriptome analysis of hESC- and iPSC-derived lentoid bodies
    Article Snippet: Total RNA was subjected to RNA-Seq library preparation using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Ipswich, MA). .. The cDNA was further converted into double-stranded DNA that was end-repaired to incorporate the specific index adapters for multiplexing, followed by a purification step and amplification for 15 cycles.

    Article Title: Cell-to-Cell Variation in Defective Virus Expression and Effects on Host Responses during Influenza Virus Infection
    Article Snippet: The conventional bulk RNA-seq library was prepared using an NEBNext Ultra II RNA library prep kit for Illumina following poly(A) mRNA enrichment. .. Viral genomic RNA (vRNA) of the virus stocks used in the infection assay was amplified using multisegment reverse transcription-PCR (M-RTPCR) ( ).

    Construct:

    Article Title: Porcine placental extract facilitates memory and learning in aged mice, et al. Porcine placental extract facilitates memory and learning in aged mice
    Article Snippet: .. The integrity of the extracted total RNA was analyzed using a Bioanalyzer (Agilent), and RNA‐seq libraries were constructed using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB). .. The libraries (control vs. 1,000 mg/kg SD‐F, each n = 3) were sequenced on an Illumina Nextseq platform.

    Article Title: Complete Genome Sequence of Iris Severe Mosaic Virus Isolated in Japan
    Article Snippet: .. A paired-end sequencing cDNA library was constructed from the extracted RNA using the NEBNext Ultra II RNA library prep kit for Illumina (New England Biolabs, USA), according to the manufacturer’s instructions except for poly(A)-tailed mRNA purification, and sequenced using a MiSeq instrument (Illumina, USA) and the MiSeq reagent kit v.2 (500 cycles). ..

    Electrophoresis:

    Article Title: Nuclear lamina integrity is required for proper spatial organization of chromatin in Drosophila
    Article Snippet: RNA quality was assessed using capillary electrophoresis with a Bioanalyzer 2100 (Agilent). .. NEBNext Ultra II RNA library preparation kit (New England Biolabs) was used for preparation of libraries following manufacturer’s instructions.

    Incubation:

    Article Title: DNA mismatch repair controls the host innate response and cell fate after influenza virus infection
    Article Snippet: H441 cells were transfected with siRNA and incubated for 48 h before being infected with WT PR8 (MOI=10). .. RNA was then prepped for RNAseq submission using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB), NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB), and NEBNext Multiplex Oligos for Illumina (NEB).

    Article Title: Listeria monocytogenes Relies on the Heme-Regulated Transporter hrtAB to Resist Heme Toxicity and Uses Heme as a Signal to Induce Transcription of lmo1634, Encoding Listeria Adhesion Protein
    Article Snippet: Briefly, beads were washed twice and hybridized with probes at 68°C for 10 min. A total of 500 ng RNA was added to the mixture and incubated at room temperature and 50°C for 5 min each, followed by separation of mRNA from rRNA, which was bound to the beads using magnetic stand. .. Preparation of cDNA fragment libraries was performed using the NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® (Illumina) with minor modifications.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Cross-site comparison of ribosomal depletion kits for Illumina RNAseq library construction
    Article Snippet: It should be noted that while heat degraded RNA is a proxy for difficult to isolate samples (eg, brain microdissections or tumors), the samples are not formalin fixed and so lack the base modifications often observed in FFPE samples [ ]. .. The Lexogen, Qiagen, Ribogone, and NEBNext kits all utilized the NEB Next Ultra II directional library generation kit to convert the RNA to Illumina libraries while the other kits used RNA library generation kits from the manufacturer of the depletion chemistry.

    Expressing:

    Article Title: Nuclear lamina integrity is required for proper spatial organization of chromatin in Drosophila
    Article Snippet: Paragraph title: Analysis of gene expression ... NEBNext Ultra II RNA library preparation kit (New England Biolabs) was used for preparation of libraries following manufacturer’s instructions.

    Article Title: Porcine placental extract facilitates memory and learning in aged mice, et al. Porcine placental extract facilitates memory and learning in aged mice
    Article Snippet: The integrity of the extracted total RNA was analyzed using a Bioanalyzer (Agilent), and RNA‐seq libraries were constructed using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB). .. Transcripts per million (TPMs) were calculated, and genes with low expression were eliminated (cutoff: TPM < 1).

    Transfection:

    Article Title: DNA mismatch repair controls the host innate response and cell fate after influenza virus infection
    Article Snippet: H441 cells were transfected with siRNA and incubated for 48 h before being infected with WT PR8 (MOI=10). .. RNA was then prepped for RNAseq submission using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB), NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB), and NEBNext Multiplex Oligos for Illumina (NEB).

    Ligation:

    Article Title: Listeria monocytogenes Relies on the Heme-Regulated Transporter hrtAB to Resist Heme Toxicity and Uses Heme as a Signal to Induce Transcription of lmo1634, Encoding Listeria Adhesion Protein
    Article Snippet: Preparation of cDNA fragment libraries was performed using the NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® (Illumina) with minor modifications. .. End repair was performed on the ds cDNA library followed by ligation of adaptors.

    Infection:

    Article Title: DNA mismatch repair controls the host innate response and cell fate after influenza virus infection
    Article Snippet: H441 cells were transfected with siRNA and incubated for 48 h before being infected with WT PR8 (MOI=10). .. RNA was then prepped for RNAseq submission using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB), NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB), and NEBNext Multiplex Oligos for Illumina (NEB).

    Article Title: Cell-to-Cell Variation in Defective Virus Expression and Effects on Host Responses during Influenza Virus Infection
    Article Snippet: The conventional bulk RNA-seq library was prepared using an NEBNext Ultra II RNA library prep kit for Illumina following poly(A) mRNA enrichment. .. Viral genomic RNA (vRNA) of the virus stocks used in the infection assay was amplified using multisegment reverse transcription-PCR (M-RTPCR) ( ).

    Generated:

    Article Title: Natural Variation in RNA m6A Methylation and Its Relationship with Translational Status
    Article Snippet: .. Libraries of RNA-seq, m6 A-seq, and polysome profiling were generated using the NEBNext Ultra II RNA Library Prep Kit (model no. E7770S; New England Biolabs) according to the manufacturer’s instructions. .. The libraries were sequenced with 150-bp paired reads on a model no. HiSeq X Ten platform (Illumina).

    Sequencing:

    Article Title: Natural Variation in RNA m6A Methylation and Its Relationship with Translational Status
    Article Snippet: Paragraph title: Library Construction and Sequencing ... Libraries of RNA-seq, m6 A-seq, and polysome profiling were generated using the NEBNext Ultra II RNA Library Prep Kit (model no. E7770S; New England Biolabs) according to the manufacturer’s instructions.

    Article Title: Middle East Respiratory Syndrome Coronavirus in Dromedaries in Ethiopia Is Antigenically Different From the Middle East Isolate EMC
    Article Snippet: Paragraph title: Sequencing Analysis ... Extracted viral RNA was reverse transcribed and tagged with index adaptors using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions.

    Article Title: DNA mismatch repair controls the host innate response and cell fate after influenza virus infection
    Article Snippet: RNA was then prepped for RNAseq submission using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB), NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB), and NEBNext Multiplex Oligos for Illumina (NEB). .. Mapping of raw reads to the human hg19 reference genome was accomplished using a custom app on the Illumina BaseSpace Sequence Hub .

    Article Title: Porcine placental extract facilitates memory and learning in aged mice, et al. Porcine placental extract facilitates memory and learning in aged mice
    Article Snippet: The integrity of the extracted total RNA was analyzed using a Bioanalyzer (Agilent), and RNA‐seq libraries were constructed using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB). .. Trimming, mapping, and read counts were performed using Mus musculus GRCm38 release‐92 as a reference sequence on CLC Genomics Workbench (CLC bio).

    Article Title: Cell-to-Cell Variation in Defective Virus Expression and Effects on Host Responses during Influenza Virus Infection
    Article Snippet: Paragraph title: Sample preparation, library construction, and sequencing. ... The conventional bulk RNA-seq library was prepared using an NEBNext Ultra II RNA library prep kit for Illumina following poly(A) mRNA enrichment.

    Article Title: Complete Genome Sequence of Iris Severe Mosaic Virus Isolated in Japan
    Article Snippet: .. A paired-end sequencing cDNA library was constructed from the extracted RNA using the NEBNext Ultra II RNA library prep kit for Illumina (New England Biolabs, USA), according to the manufacturer’s instructions except for poly(A)-tailed mRNA purification, and sequenced using a MiSeq instrument (Illumina, USA) and the MiSeq reagent kit v.2 (500 cycles). ..

    Multiplexing:

    Article Title: Comparative transcriptome analysis of hESC- and iPSC-derived lentoid bodies
    Article Snippet: Total RNA was subjected to RNA-Seq library preparation using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Ipswich, MA). .. The cDNA was further converted into double-stranded DNA that was end-repaired to incorporate the specific index adapters for multiplexing, followed by a purification step and amplification for 15 cycles.

    Nucleic Acid Electrophoresis:

    Article Title: Middle East Respiratory Syndrome Coronavirus in Dromedaries in Ethiopia Is Antigenically Different From the Middle East Isolate EMC
    Article Snippet: Extracted viral RNA was reverse transcribed and tagged with index adaptors using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. .. The resulting cDNA libraries were verified by gel electrophoresis and quantified using a Quantus Fluorometer (Promega, Madison, WI, USA).

    RNA Sequencing Assay:

    Article Title: Natural Variation in RNA m6A Methylation and Its Relationship with Translational Status
    Article Snippet: .. Libraries of RNA-seq, m6 A-seq, and polysome profiling were generated using the NEBNext Ultra II RNA Library Prep Kit (model no. E7770S; New England Biolabs) according to the manufacturer’s instructions. .. The libraries were sequenced with 150-bp paired reads on a model no. HiSeq X Ten platform (Illumina).

    Article Title: A molecular switch from STAT2-IRF9 to ISGF3 underlies interferon-induced gene transcription
    Article Snippet: Paragraph title: RNA-seq ... For library preparation, the NEBNext® Poly(A) mRNA Magnetic Isolation Module together with the NEBNext Ultra II RNA Library Prep Kit from NEB Catalog # E7770L was used according to the manufacturer’s protocol.

    Article Title: Middle East Respiratory Syndrome Coronavirus in Dromedaries in Ethiopia Is Antigenically Different From the Middle East Isolate EMC
    Article Snippet: Sequencing Analysis The nearly complete genome of Ethiopian MERS-CoV was analyzed by RNA sequencing. .. Extracted viral RNA was reverse transcribed and tagged with index adaptors using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions.

    Article Title: Comparative transcriptome analysis of hESC- and iPSC-derived lentoid bodies
    Article Snippet: .. Total RNA was subjected to RNA-Seq library preparation using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Ipswich, MA). .. Approximately, 1.0 µg of high-quality total RNA (≥8.0 RIN Score) was used for polyadenylated RNA selection using oligo dT beads, followed by the fragmentation of selected polyadenylated RNA.

    Article Title: Porcine placental extract facilitates memory and learning in aged mice, et al. Porcine placental extract facilitates memory and learning in aged mice
    Article Snippet: .. The integrity of the extracted total RNA was analyzed using a Bioanalyzer (Agilent), and RNA‐seq libraries were constructed using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB). .. The libraries (control vs. 1,000 mg/kg SD‐F, each n = 3) were sequenced on an Illumina Nextseq platform.

    Article Title: Cell-to-Cell Variation in Defective Virus Expression and Effects on Host Responses during Influenza Virus Infection
    Article Snippet: .. The conventional bulk RNA-seq library was prepared using an NEBNext Ultra II RNA library prep kit for Illumina following poly(A) mRNA enrichment. .. Libraries were multiplexed and sequenced on an Illumina NextSeq 500 in HighOutput 2× 75-bp mode (v3).

    RNA HS Assay:

    Article Title: Listeria monocytogenes Relies on the Heme-Regulated Transporter hrtAB to Resist Heme Toxicity and Uses Heme as a Signal to Induce Transcription of lmo1634, Encoding Listeria Adhesion Protein
    Article Snippet: Concentration of RNA was measured using fluorescence-based QubitTM RNA HS Assay (Thermo Fisher Scientific). .. Preparation of cDNA fragment libraries was performed using the NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® (Illumina) with minor modifications.

    Fluorescence:

    Article Title: Listeria monocytogenes Relies on the Heme-Regulated Transporter hrtAB to Resist Heme Toxicity and Uses Heme as a Signal to Induce Transcription of lmo1634, Encoding Listeria Adhesion Protein
    Article Snippet: Concentration of RNA was measured using fluorescence-based QubitTM RNA HS Assay (Thermo Fisher Scientific). .. Preparation of cDNA fragment libraries was performed using the NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® (Illumina) with minor modifications.

    Magnetic Beads:

    Article Title: Nuclear lamina integrity is required for proper spatial organization of chromatin in Drosophila
    Article Snippet: Poly(A)+ RNA was extracted from total RNA using oligo(dT) magnetic beads (Thermo Fisher Scientific). .. NEBNext Ultra II RNA library preparation kit (New England Biolabs) was used for preparation of libraries following manufacturer’s instructions.

    Isolation:

    Article Title: A molecular switch from STAT2-IRF9 to ISGF3 underlies interferon-induced gene transcription
    Article Snippet: .. For library preparation, the NEBNext® Poly(A) mRNA Magnetic Isolation Module together with the NEBNext Ultra II RNA Library Prep Kit from NEB Catalog # E7770L was used according to the manufacturer’s protocol. .. The samples were quality checked and sequenced at the Vienna Biocenter Core Facilities NGS Unit.

    Article Title: Miniaturization and optimization of 384-well compatible RNA sequencing library preparation
    Article Snippet: The protocol described herein is independent of nucleic acid isolation methods, and for the purpose of this work, we assume an input of isolated total RNA. .. To optimize miniaturization of our laboratory’s current library preparation protocol, we prepared libraries from varying concentrations of HeLa RNA using the New England Biolabs Ultra II RNA Library Prep Kit (E7770S/L).

    Article Title: DNA mismatch repair controls the host innate response and cell fate after influenza virus infection
    Article Snippet: .. RNA was then prepped for RNAseq submission using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB), NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB), and NEBNext Multiplex Oligos for Illumina (NEB). .. Mapping of raw reads to the human hg19 reference genome was accomplished using a custom app on the Illumina BaseSpace Sequence Hub .

    Article Title: Nuclear lamina integrity is required for proper spatial organization of chromatin in Drosophila
    Article Snippet: Analysis of gene expression Total RNA was isolated from Lam-KD or control S2 cells using Trizol reagent (Invitrogen), and contaminating DNA was removed by DNase I treatment. .. NEBNext Ultra II RNA library preparation kit (New England Biolabs) was used for preparation of libraries following manufacturer’s instructions.

    Article Title: Comparative transcriptome analysis of hESC- and iPSC-derived lentoid bodies
    Article Snippet: Total RNA was isolated from each sample using the Quick-RNA™ MicroPrep kit (Zymo Research; Irvine, CA). .. Total RNA was subjected to RNA-Seq library preparation using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Ipswich, MA).

    Article Title: Porcine placental extract facilitates memory and learning in aged mice, et al. Porcine placental extract facilitates memory and learning in aged mice
    Article Snippet: .. The integrity of the extracted total RNA was analyzed using a Bioanalyzer (Agilent), and RNA‐seq libraries were constructed using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB). .. The libraries (control vs. 1,000 mg/kg SD‐F, each n = 3) were sequenced on an Illumina Nextseq platform.

    Article Title: Complete Genome Sequence of Iris Severe Mosaic Virus Isolated in Japan
    Article Snippet: Here, we report the first complete genome sequence of ISMV isolated in Japan. .. A paired-end sequencing cDNA library was constructed from the extracted RNA using the NEBNext Ultra II RNA library prep kit for Illumina (New England Biolabs, USA), according to the manufacturer’s instructions except for poly(A)-tailed mRNA purification, and sequenced using a MiSeq instrument (Illumina, USA) and the MiSeq reagent kit v.2 (500 cycles).

    Purification:

    Article Title: Comparative transcriptome analysis of hESC- and iPSC-derived lentoid bodies
    Article Snippet: Total RNA was subjected to RNA-Seq library preparation using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Ipswich, MA). .. The cDNA was further converted into double-stranded DNA that was end-repaired to incorporate the specific index adapters for multiplexing, followed by a purification step and amplification for 15 cycles.

    Article Title: Listeria monocytogenes Relies on the Heme-Regulated Transporter hrtAB to Resist Heme Toxicity and Uses Heme as a Signal to Induce Transcription of lmo1634, Encoding Listeria Adhesion Protein
    Article Snippet: Enriched mRNA was purified by Zymo-Spin column (Zymo Research) and run on Labchip GX II bioanalyzer (Perkin Elmer) to confirm reduction of rRNA. .. Preparation of cDNA fragment libraries was performed using the NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® (Illumina) with minor modifications.

    Article Title: Complete Genome Sequence of Iris Severe Mosaic Virus Isolated in Japan
    Article Snippet: .. A paired-end sequencing cDNA library was constructed from the extracted RNA using the NEBNext Ultra II RNA library prep kit for Illumina (New England Biolabs, USA), according to the manufacturer’s instructions except for poly(A)-tailed mRNA purification, and sequenced using a MiSeq instrument (Illumina, USA) and the MiSeq reagent kit v.2 (500 cycles). ..

    cDNA Library Assay:

    Article Title: Listeria monocytogenes Relies on the Heme-Regulated Transporter hrtAB to Resist Heme Toxicity and Uses Heme as a Signal to Induce Transcription of lmo1634, Encoding Listeria Adhesion Protein
    Article Snippet: Preparation of cDNA fragment libraries was performed using the NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® (Illumina) with minor modifications. .. End repair was performed on the ds cDNA library followed by ligation of adaptors.

    Article Title: Complete Genome Sequence of Iris Severe Mosaic Virus Isolated in Japan
    Article Snippet: .. A paired-end sequencing cDNA library was constructed from the extracted RNA using the NEBNext Ultra II RNA library prep kit for Illumina (New England Biolabs, USA), according to the manufacturer’s instructions except for poly(A)-tailed mRNA purification, and sequenced using a MiSeq instrument (Illumina, USA) and the MiSeq reagent kit v.2 (500 cycles). ..

    Software:

    Article Title: Middle East Respiratory Syndrome Coronavirus in Dromedaries in Ethiopia Is Antigenically Different From the Middle East Isolate EMC
    Article Snippet: Extracted viral RNA was reverse transcribed and tagged with index adaptors using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. .. Sequence reads were trimmed and mapped to the EMC isolate (GenBank accession no. JX869059) reference genome using CLC Genomic Workbench 9.0.1 software (CLC bio, Aarhus, Denmark) with the default settings, except for trimming (Q ≥ 20 and read length ≥ 75 bp).

    Article Title: Complete Genome Sequence of Iris Severe Mosaic Virus Isolated in Japan
    Article Snippet: A paired-end sequencing cDNA library was constructed from the extracted RNA using the NEBNext Ultra II RNA library prep kit for Illumina (New England Biolabs, USA), according to the manufacturer’s instructions except for poly(A)-tailed mRNA purification, and sequenced using a MiSeq instrument (Illumina, USA) and the MiSeq reagent kit v.2 (500 cycles). .. After quality control and adapter trimming using Trimmomatic v.0.36 software ( ) (ILLUMINACLIP:adapters.fa:2:30:10, LEADING:20, TRAILING:20 SLIDINGWINDOW:4:15, MINLEN:36, and other parameters with default settings), the reads were de novo assembled using Trinity v.2.6.6 software ( ) with default settings.

    Real-time Polymerase Chain Reaction:

    Article Title: Nuclear lamina integrity is required for proper spatial organization of chromatin in Drosophila
    Article Snippet: NEBNext Ultra II RNA library preparation kit (New England Biolabs) was used for preparation of libraries following manufacturer’s instructions. .. Libraries from two biological replicates of Lam-KD or control S2 cells were quantified using a Qubit fluorometer and quantitative PCR, and sequenced on the Illumina NextSeq resulting in 8.4–9.4 × 106 80-nt single-end reads.

    Multiplex Assay:

    Article Title: DNA mismatch repair controls the host innate response and cell fate after influenza virus infection
    Article Snippet: .. RNA was then prepped for RNAseq submission using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB), NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB), and NEBNext Multiplex Oligos for Illumina (NEB). .. Mapping of raw reads to the human hg19 reference genome was accomplished using a custom app on the Illumina BaseSpace Sequence Hub .

    Selection:

    Article Title: Comparative transcriptome analysis of hESC- and iPSC-derived lentoid bodies
    Article Snippet: Total RNA was subjected to RNA-Seq library preparation using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Ipswich, MA). .. Approximately, 1.0 µg of high-quality total RNA (≥8.0 RIN Score) was used for polyadenylated RNA selection using oligo dT beads, followed by the fragmentation of selected polyadenylated RNA.

    Article Title: Listeria monocytogenes Relies on the Heme-Regulated Transporter hrtAB to Resist Heme Toxicity and Uses Heme as a Signal to Induce Transcription of lmo1634, Encoding Listeria Adhesion Protein
    Article Snippet: Preparation of cDNA fragment libraries was performed using the NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® (Illumina) with minor modifications. .. Double-stranded cDNA (ds cDNA) was purified using NucleoMag (Macherey nagel) SPRI selection.

    Sample Prep:

    Article Title: Cell-to-Cell Variation in Defective Virus Expression and Effects on Host Responses during Influenza Virus Infection
    Article Snippet: Paragraph title: Sample preparation, library construction, and sequencing. ... The conventional bulk RNA-seq library was prepared using an NEBNext Ultra II RNA library prep kit for Illumina following poly(A) mRNA enrichment.

    Next-Generation Sequencing:

    Article Title: A molecular switch from STAT2-IRF9 to ISGF3 underlies interferon-induced gene transcription
    Article Snippet: For library preparation, the NEBNext® Poly(A) mRNA Magnetic Isolation Module together with the NEBNext Ultra II RNA Library Prep Kit from NEB Catalog # E7770L was used according to the manufacturer’s protocol. .. The samples were quality checked and sequenced at the Vienna Biocenter Core Facilities NGS Unit.

    Article Title: Miniaturization and optimization of 384-well compatible RNA sequencing library preparation
    Article Snippet: Methods and results All next generation sequencing workflows begin with isolation of nucleic acid; however, such protocols are highly dependent on the source of the sample and desired product, such as DNA, RNA, mRNA, cell-free RNA, and so on. .. To optimize miniaturization of our laboratory’s current library preparation protocol, we prepared libraries from varying concentrations of HeLa RNA using the New England Biolabs Ultra II RNA Library Prep Kit (E7770S/L).

    Article Title: Comparative transcriptome analysis of hESC- and iPSC-derived lentoid bodies
    Article Snippet: Paragraph title: RNA-Seq library preparation and next-generation sequencing ... Total RNA was subjected to RNA-Seq library preparation using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Ipswich, MA).

    Laser Capture Microdissection:

    Article Title: Nuclear lamina integrity is required for proper spatial organization of chromatin in Drosophila
    Article Snippet: Analysis of gene expression Total RNA was isolated from Lam-KD or control S2 cells using Trizol reagent (Invitrogen), and contaminating DNA was removed by DNase I treatment. .. NEBNext Ultra II RNA library preparation kit (New England Biolabs) was used for preparation of libraries following manufacturer’s instructions.

    Spectrophotometry:

    Article Title: Comparative transcriptome analysis of hESC- and iPSC-derived lentoid bodies
    Article Snippet: The extracted RNA was examined using a NanoDrop Lite spectrophotometer (Thermo Fisher Scientific) and RNA 6000 Pico kit on an Agilent 2100 Bioanalyzer (Agilent; Palo Alto, CA), respectively. .. Total RNA was subjected to RNA-Seq library preparation using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Ipswich, MA).

    Concentration Assay:

    Article Title: Listeria monocytogenes Relies on the Heme-Regulated Transporter hrtAB to Resist Heme Toxicity and Uses Heme as a Signal to Induce Transcription of lmo1634, Encoding Listeria Adhesion Protein
    Article Snippet: Concentration of RNA was measured using fluorescence-based QubitTM RNA HS Assay (Thermo Fisher Scientific). .. Preparation of cDNA fragment libraries was performed using the NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® (Illumina) with minor modifications.

    Lysis:

    Article Title: A molecular switch from STAT2-IRF9 to ISGF3 underlies interferon-induced gene transcription
    Article Snippet: Seven milliliters of Qiazol Lysis Reagent (Qiagen) were added per 15-cm dish. .. For library preparation, the NEBNext® Poly(A) mRNA Magnetic Isolation Module together with the NEBNext Ultra II RNA Library Prep Kit from NEB Catalog # E7770L was used according to the manufacturer’s protocol.

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  • 99
    New England Biolabs ultratm ii directional rna library prep kit
    Ultratm Ii Directional Rna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultratm ii directional rna library prep kit/product/New England Biolabs
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ultratm ii directional rna library prep kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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