ultrapuretm  (Thermo Fisher)


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  • 99
    Name:
    UltraPure Phenol
    Description:
    UltraPure Phenol is used in the purification of nucleic acids In a mixture of phenol and buffered aqueous solution proteins are denatured and collect at the interphase while most nucleic acids remain in the aqueous phase UltraPure Phenol contains no preservatives It is redistilled and packaged under a dry inert gas in shatter resistant plastic coated amber bottles Performance and Quality Testing Color of water saturated samples is evaluated after incubation at 2°C to 8°C
    Catalog Number:
    15509037
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|DNA Extraction|General gDNA Purification Reagents & Accessories|Genomic DNA Purification|Miniprep|Plasmid DNA Purification|RNA Extraction|Total RNA Isolation
    Category:
    Lab Reagents and Chemicals
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    Structured Review

    Thermo Fisher ultrapuretm
    UltraPure Phenol is used in the purification of nucleic acids In a mixture of phenol and buffered aqueous solution proteins are denatured and collect at the interphase while most nucleic acids remain in the aqueous phase UltraPure Phenol contains no preservatives It is redistilled and packaged under a dry inert gas in shatter resistant plastic coated amber bottles Performance and Quality Testing Color of water saturated samples is evaluated after incubation at 2°C to 8°C
    https://www.bioz.com/result/ultrapuretm/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ultrapuretm - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Analysis of mtDNA/nDNA ratio in mice
    Article Snippet: .. Mouse cells EDTA (Applichem, A2937,0500) NaCl (Sigma, 71380) 20% SDS solution (Applichem, A0675,0500) Tris(Hydroxymethyl aminomethane) (Biosolve, 0020092391BS) 37% Hydrochloric acid (Applichem, A0659,1000GL) 3 M NaCl solution (see reagents and solutions ) 0.5 M EDTA solution ( see reagents and solutions ) 1 M Tris-HCl solution ( see reagents and solutions ) TE buffer ( see reagents and solutions ) Lysis buffer ( see reagents and solutions ) Ammonium acetate (Fischer, A/3440/50) 7.5 M Ammonium acetate solution ( see reagents and solutions ) Ultrapure™ Phenol: Chloroform: isoamyl alcohol mix (25:24:1, v/v) (Thermofischer, 15593031) 70% Ethanol (Reactolab, 96170) Proteinase K recombinant, PCR Grade (20 mg/ml, RPROTK-RO ROCHE, 000000003115836001) RNase A (5 mg/ml, Thermofisher, EN0531) PBS pH7.4 (1X) (gibco, Ref.no.10010-015) Centrifuge Tubes 15-ml polypropylene Falcon tubes, 2-ml centrifuge tubes, 1.5-ml centrifuge tubes NanoDrop 2000 (UV-Vis Spectrophotometer) qPCR Syber Green Mix (LightCycler ® 480 SYBR Green I Master - Roche Life Science, 4887352001) Forward and reverse primers (Invitrogen) 16S rRNA forward and reverse primers FWD : 5’-CCGCAAGGGAAAGATGAAAGAC-3’ REV : 5’-TCGTTTGGTTTCGGGGTTTC-3’ ND1 forward and reverse primers FWD : 5’-CTAGCAGAAACAAACCGGGC-3’ REV : 5’-CCGGCTGCGTATTCTACGTT-3’ HK2 forward and reverse primers FWD : 5’-GCCAGCCTCTCCTGATTTTAGTGT-3’ REV : 5’-GGGAACACAAAAGACCTCTTCTGG-3’ 384 well plates Plate seals (ThermalSeal RTS™ Sealing Films, Z734438) Double-distilled or Milli Q water Apparatus Sterile filter pipette tips, sterile 1.5-mL screw top microcentrifuge tubes ..

    Isolation:

    Article Title: Oxidized mitochondrial nucleoids released by neutrophils drive type I interferon production in human lupus
    Article Snippet: .. Isolation of DNA from supernatants and analysis Supernatants (500 µl) were digested with PK (1 mg/ml) for 60 min at 60°C and then mixed with 1 vol UltraPure Phenol/Chloroform/Isoamyl Alcohol (25:24:1 vol/vol/vol; Invitrogen). ..

    Transferring:

    Article Title: Analysis of mtDNA/nDNA ratio in mice
    Article Snippet: .. Mouse cells EDTA (Applichem, A2937,0500) NaCl (Sigma, 71380) 20% SDS solution (Applichem, A0675,0500) Tris(Hydroxymethyl aminomethane) (Biosolve, 0020092391BS) 37% Hydrochloric acid (Applichem, A0659,1000GL) 3 M NaCl solution (see reagents and solutions ) 0.5 M EDTA solution ( see reagents and solutions ) 1 M Tris-HCl solution ( see reagents and solutions ) TE buffer ( see reagents and solutions ) Lysis buffer ( see reagents and solutions ) Ammonium acetate (Fischer, A/3440/50) 7.5 M Ammonium acetate solution ( see reagents and solutions ) Ultrapure™ Phenol: Chloroform: isoamyl alcohol mix (25:24:1, v/v) (Thermofischer, 15593031) 70% Ethanol (Reactolab, 96170) Proteinase K recombinant, PCR Grade (20 mg/ml, RPROTK-RO ROCHE, 000000003115836001) RNase A (5 mg/ml, Thermofisher, EN0531) PBS pH7.4 (1X) (gibco, Ref.no.10010-015) Centrifuge Tubes 15-ml polypropylene Falcon tubes, 2-ml centrifuge tubes, 1.5-ml centrifuge tubes NanoDrop 2000 (UV-Vis Spectrophotometer) qPCR Syber Green Mix (LightCycler ® 480 SYBR Green I Master - Roche Life Science, 4887352001) Forward and reverse primers (Invitrogen) 16S rRNA forward and reverse primers FWD : 5’-CCGCAAGGGAAAGATGAAAGAC-3’ REV : 5’-TCGTTTGGTTTCGGGGTTTC-3’ ND1 forward and reverse primers FWD : 5’-CTAGCAGAAACAAACCGGGC-3’ REV : 5’-CCGGCTGCGTATTCTACGTT-3’ HK2 forward and reverse primers FWD : 5’-GCCAGCCTCTCCTGATTTTAGTGT-3’ REV : 5’-GGGAACACAAAAGACCTCTTCTGG-3’ 384 well plates Plate seals (ThermalSeal RTS™ Sealing Films, Z734438) Double-distilled or Milli Q water Apparatus Sterile filter pipette tips, sterile 1.5-mL screw top microcentrifuge tubes ..

    Spectrophotometry:

    Article Title: Analysis of mtDNA/nDNA ratio in mice
    Article Snippet: .. Mouse cells EDTA (Applichem, A2937,0500) NaCl (Sigma, 71380) 20% SDS solution (Applichem, A0675,0500) Tris(Hydroxymethyl aminomethane) (Biosolve, 0020092391BS) 37% Hydrochloric acid (Applichem, A0659,1000GL) 3 M NaCl solution (see reagents and solutions ) 0.5 M EDTA solution ( see reagents and solutions ) 1 M Tris-HCl solution ( see reagents and solutions ) TE buffer ( see reagents and solutions ) Lysis buffer ( see reagents and solutions ) Ammonium acetate (Fischer, A/3440/50) 7.5 M Ammonium acetate solution ( see reagents and solutions ) Ultrapure™ Phenol: Chloroform: isoamyl alcohol mix (25:24:1, v/v) (Thermofischer, 15593031) 70% Ethanol (Reactolab, 96170) Proteinase K recombinant, PCR Grade (20 mg/ml, RPROTK-RO ROCHE, 000000003115836001) RNase A (5 mg/ml, Thermofisher, EN0531) PBS pH7.4 (1X) (gibco, Ref.no.10010-015) Centrifuge Tubes 15-ml polypropylene Falcon tubes, 2-ml centrifuge tubes, 1.5-ml centrifuge tubes NanoDrop 2000 (UV-Vis Spectrophotometer) qPCR Syber Green Mix (LightCycler ® 480 SYBR Green I Master - Roche Life Science, 4887352001) Forward and reverse primers (Invitrogen) 16S rRNA forward and reverse primers FWD : 5’-CCGCAAGGGAAAGATGAAAGAC-3’ REV : 5’-TCGTTTGGTTTCGGGGTTTC-3’ ND1 forward and reverse primers FWD : 5’-CTAGCAGAAACAAACCGGGC-3’ REV : 5’-CCGGCTGCGTATTCTACGTT-3’ HK2 forward and reverse primers FWD : 5’-GCCAGCCTCTCCTGATTTTAGTGT-3’ REV : 5’-GGGAACACAAAAGACCTCTTCTGG-3’ 384 well plates Plate seals (ThermalSeal RTS™ Sealing Films, Z734438) Double-distilled or Milli Q water Apparatus Sterile filter pipette tips, sterile 1.5-mL screw top microcentrifuge tubes ..

    Purification:

    Article Title: Widespread Misinterpretable ChIP-seq Bias in Yeast
    Article Snippet: .. The crosslinks were reversed by heating at 65°C for 12-16 hours and the immunoprecipitated DNA was purified using UltraPure Phenol:Chloroform:Isoamyl alcohol (25:24:1 v/v, Invitrogen). .. Sequencing library preparation Sequencing library preparation with ChIP-ed DNA and input DNA was carried out by following either the NEB ChIP-seq library preparation for Illumina (New England Biolabs) or the SOLiD V3 barcoded fragment library preparation protocol (Life Technologies).

    Article Title: Antiviral immunity via RIG-I-mediated recognition of RNA bearing 5′-diphosphates
    Article Snippet: .. Poly(I:C) samples were purified using Ultra Pure™ phenol:chloroform:isoamylalcohol (25:24:1) (Life Technologies) and precipitated using ethanol before being treated with CIP and re-purified as done following RNase III treatment. .. A fraction of the samples were electrophoresed in 0.8% agarose gels and visualized using ethidium bromide, whereas another fraction was transfected into SV40 large T antigen-immortalized MDA5−/− or RIG−/− MEFs.

    Article Title: HPV18 Utilizes Two Alternative Branch Sites for E6*I Splicing to Produce E7 Protein
    Article Snippet: .. Resulting RNA was purified by ultrapure phenol:chloroform:isoamyl alcohol (25:24:1, v/v, Thermo Fisher Scientific) extraction, precipitated, and dissolved in DEPC-treated water. .. Alternatively, the in vitro transcribed RNA was radiolabeled by addition of 60 µCi [α-32 P]-rGTP (Perkin Elmer) into the transcription reaction containing reduced level (0.05 mmol/L) of cold rGTP.

    SYBR Green Assay:

    Article Title: Analysis of mtDNA/nDNA ratio in mice
    Article Snippet: .. Mouse cells EDTA (Applichem, A2937,0500) NaCl (Sigma, 71380) 20% SDS solution (Applichem, A0675,0500) Tris(Hydroxymethyl aminomethane) (Biosolve, 0020092391BS) 37% Hydrochloric acid (Applichem, A0659,1000GL) 3 M NaCl solution (see reagents and solutions ) 0.5 M EDTA solution ( see reagents and solutions ) 1 M Tris-HCl solution ( see reagents and solutions ) TE buffer ( see reagents and solutions ) Lysis buffer ( see reagents and solutions ) Ammonium acetate (Fischer, A/3440/50) 7.5 M Ammonium acetate solution ( see reagents and solutions ) Ultrapure™ Phenol: Chloroform: isoamyl alcohol mix (25:24:1, v/v) (Thermofischer, 15593031) 70% Ethanol (Reactolab, 96170) Proteinase K recombinant, PCR Grade (20 mg/ml, RPROTK-RO ROCHE, 000000003115836001) RNase A (5 mg/ml, Thermofisher, EN0531) PBS pH7.4 (1X) (gibco, Ref.no.10010-015) Centrifuge Tubes 15-ml polypropylene Falcon tubes, 2-ml centrifuge tubes, 1.5-ml centrifuge tubes NanoDrop 2000 (UV-Vis Spectrophotometer) qPCR Syber Green Mix (LightCycler ® 480 SYBR Green I Master - Roche Life Science, 4887352001) Forward and reverse primers (Invitrogen) 16S rRNA forward and reverse primers FWD : 5’-CCGCAAGGGAAAGATGAAAGAC-3’ REV : 5’-TCGTTTGGTTTCGGGGTTTC-3’ ND1 forward and reverse primers FWD : 5’-CTAGCAGAAACAAACCGGGC-3’ REV : 5’-CCGGCTGCGTATTCTACGTT-3’ HK2 forward and reverse primers FWD : 5’-GCCAGCCTCTCCTGATTTTAGTGT-3’ REV : 5’-GGGAACACAAAAGACCTCTTCTGG-3’ 384 well plates Plate seals (ThermalSeal RTS™ Sealing Films, Z734438) Double-distilled or Milli Q water Apparatus Sterile filter pipette tips, sterile 1.5-mL screw top microcentrifuge tubes ..

    Immunoprecipitation:

    Article Title: Widespread Misinterpretable ChIP-seq Bias in Yeast
    Article Snippet: .. The crosslinks were reversed by heating at 65°C for 12-16 hours and the immunoprecipitated DNA was purified using UltraPure Phenol:Chloroform:Isoamyl alcohol (25:24:1 v/v, Invitrogen). .. Sequencing library preparation Sequencing library preparation with ChIP-ed DNA and input DNA was carried out by following either the NEB ChIP-seq library preparation for Illumina (New England Biolabs) or the SOLiD V3 barcoded fragment library preparation protocol (Life Technologies).

    Incubation:

    Article Title: Systematic analysis of microRNA expression of RNA extracted from fresh frozen and formalin-fixed paraffin-embedded samples
    Article Snippet: .. Fifteen microliters of DNase treatment mix (2 μL RQ1 RNase-Free DNase, 11 μL RQ1 DNase 10× reaction buffer, and 2 μL RNasin Plus RNase inhibitor; Promega) were incubated with each sample in 100 μL volume at 37°C for 30 min. We then added 115 μL ultrapure phenol:chloroform:isoamyl alcohol (25:24:1, Invitrogen Inc.). ..

    Polymerase Chain Reaction:

    Article Title: Analysis of mtDNA/nDNA ratio in mice
    Article Snippet: .. Mouse cells EDTA (Applichem, A2937,0500) NaCl (Sigma, 71380) 20% SDS solution (Applichem, A0675,0500) Tris(Hydroxymethyl aminomethane) (Biosolve, 0020092391BS) 37% Hydrochloric acid (Applichem, A0659,1000GL) 3 M NaCl solution (see reagents and solutions ) 0.5 M EDTA solution ( see reagents and solutions ) 1 M Tris-HCl solution ( see reagents and solutions ) TE buffer ( see reagents and solutions ) Lysis buffer ( see reagents and solutions ) Ammonium acetate (Fischer, A/3440/50) 7.5 M Ammonium acetate solution ( see reagents and solutions ) Ultrapure™ Phenol: Chloroform: isoamyl alcohol mix (25:24:1, v/v) (Thermofischer, 15593031) 70% Ethanol (Reactolab, 96170) Proteinase K recombinant, PCR Grade (20 mg/ml, RPROTK-RO ROCHE, 000000003115836001) RNase A (5 mg/ml, Thermofisher, EN0531) PBS pH7.4 (1X) (gibco, Ref.no.10010-015) Centrifuge Tubes 15-ml polypropylene Falcon tubes, 2-ml centrifuge tubes, 1.5-ml centrifuge tubes NanoDrop 2000 (UV-Vis Spectrophotometer) qPCR Syber Green Mix (LightCycler ® 480 SYBR Green I Master - Roche Life Science, 4887352001) Forward and reverse primers (Invitrogen) 16S rRNA forward and reverse primers FWD : 5’-CCGCAAGGGAAAGATGAAAGAC-3’ REV : 5’-TCGTTTGGTTTCGGGGTTTC-3’ ND1 forward and reverse primers FWD : 5’-CTAGCAGAAACAAACCGGGC-3’ REV : 5’-CCGGCTGCGTATTCTACGTT-3’ HK2 forward and reverse primers FWD : 5’-GCCAGCCTCTCCTGATTTTAGTGT-3’ REV : 5’-GGGAACACAAAAGACCTCTTCTGG-3’ 384 well plates Plate seals (ThermalSeal RTS™ Sealing Films, Z734438) Double-distilled or Milli Q water Apparatus Sterile filter pipette tips, sterile 1.5-mL screw top microcentrifuge tubes ..

    Lysis:

    Article Title: Analysis of mtDNA/nDNA ratio in mice
    Article Snippet: .. Mouse cells EDTA (Applichem, A2937,0500) NaCl (Sigma, 71380) 20% SDS solution (Applichem, A0675,0500) Tris(Hydroxymethyl aminomethane) (Biosolve, 0020092391BS) 37% Hydrochloric acid (Applichem, A0659,1000GL) 3 M NaCl solution (see reagents and solutions ) 0.5 M EDTA solution ( see reagents and solutions ) 1 M Tris-HCl solution ( see reagents and solutions ) TE buffer ( see reagents and solutions ) Lysis buffer ( see reagents and solutions ) Ammonium acetate (Fischer, A/3440/50) 7.5 M Ammonium acetate solution ( see reagents and solutions ) Ultrapure™ Phenol: Chloroform: isoamyl alcohol mix (25:24:1, v/v) (Thermofischer, 15593031) 70% Ethanol (Reactolab, 96170) Proteinase K recombinant, PCR Grade (20 mg/ml, RPROTK-RO ROCHE, 000000003115836001) RNase A (5 mg/ml, Thermofisher, EN0531) PBS pH7.4 (1X) (gibco, Ref.no.10010-015) Centrifuge Tubes 15-ml polypropylene Falcon tubes, 2-ml centrifuge tubes, 1.5-ml centrifuge tubes NanoDrop 2000 (UV-Vis Spectrophotometer) qPCR Syber Green Mix (LightCycler ® 480 SYBR Green I Master - Roche Life Science, 4887352001) Forward and reverse primers (Invitrogen) 16S rRNA forward and reverse primers FWD : 5’-CCGCAAGGGAAAGATGAAAGAC-3’ REV : 5’-TCGTTTGGTTTCGGGGTTTC-3’ ND1 forward and reverse primers FWD : 5’-CTAGCAGAAACAAACCGGGC-3’ REV : 5’-CCGGCTGCGTATTCTACGTT-3’ HK2 forward and reverse primers FWD : 5’-GCCAGCCTCTCCTGATTTTAGTGT-3’ REV : 5’-GGGAACACAAAAGACCTCTTCTGG-3’ 384 well plates Plate seals (ThermalSeal RTS™ Sealing Films, Z734438) Double-distilled or Milli Q water Apparatus Sterile filter pipette tips, sterile 1.5-mL screw top microcentrifuge tubes ..

    Recombinant:

    Article Title: Analysis of mtDNA/nDNA ratio in mice
    Article Snippet: .. Mouse cells EDTA (Applichem, A2937,0500) NaCl (Sigma, 71380) 20% SDS solution (Applichem, A0675,0500) Tris(Hydroxymethyl aminomethane) (Biosolve, 0020092391BS) 37% Hydrochloric acid (Applichem, A0659,1000GL) 3 M NaCl solution (see reagents and solutions ) 0.5 M EDTA solution ( see reagents and solutions ) 1 M Tris-HCl solution ( see reagents and solutions ) TE buffer ( see reagents and solutions ) Lysis buffer ( see reagents and solutions ) Ammonium acetate (Fischer, A/3440/50) 7.5 M Ammonium acetate solution ( see reagents and solutions ) Ultrapure™ Phenol: Chloroform: isoamyl alcohol mix (25:24:1, v/v) (Thermofischer, 15593031) 70% Ethanol (Reactolab, 96170) Proteinase K recombinant, PCR Grade (20 mg/ml, RPROTK-RO ROCHE, 000000003115836001) RNase A (5 mg/ml, Thermofisher, EN0531) PBS pH7.4 (1X) (gibco, Ref.no.10010-015) Centrifuge Tubes 15-ml polypropylene Falcon tubes, 2-ml centrifuge tubes, 1.5-ml centrifuge tubes NanoDrop 2000 (UV-Vis Spectrophotometer) qPCR Syber Green Mix (LightCycler ® 480 SYBR Green I Master - Roche Life Science, 4887352001) Forward and reverse primers (Invitrogen) 16S rRNA forward and reverse primers FWD : 5’-CCGCAAGGGAAAGATGAAAGAC-3’ REV : 5’-TCGTTTGGTTTCGGGGTTTC-3’ ND1 forward and reverse primers FWD : 5’-CTAGCAGAAACAAACCGGGC-3’ REV : 5’-CCGGCTGCGTATTCTACGTT-3’ HK2 forward and reverse primers FWD : 5’-GCCAGCCTCTCCTGATTTTAGTGT-3’ REV : 5’-GGGAACACAAAAGACCTCTTCTGG-3’ 384 well plates Plate seals (ThermalSeal RTS™ Sealing Films, Z734438) Double-distilled or Milli Q water Apparatus Sterile filter pipette tips, sterile 1.5-mL screw top microcentrifuge tubes ..

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    Thermo Fisher ultrapure dnase rnase free distilled water
    Ultrapure Dnase Rnase Free Distilled Water, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher recombinant proteins ultrapuretm agarose thermo fisher scientific
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