ultrapuretm phenol cloroform isoamyl alcohol  (Thermo Fisher)


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    Name:
    UltraPure Phenol Chloroform Isoamyl Alcohol 25 24 1 v v
    Description:
    UltraPure Phenol Chloroform Isoamyl Alcohol 25 24 1 v v is used in the purification of nucleic acids This reagent consists of highly pure chloroform isoamyl alcohol and UltraPure Phenol saturated with Tris HCl Using Phenol Chloroform Isoamyl AlcoholWhen mixtures are extracted with Phenol Chloroform Isoamyl Alcohol proteins are denatured and collected in the organic phase or at the interphase while nucleic acids remain in the aqueous phase UltraPure Phenol Chloroform lsoamyl Alcohol contains no preservatives It is packaged under an inert gas in shatter resistant plastic coated amber bottles Performance and quality testingThe appearance of the solution is evaluated at room temperature No RNase or DNase activity is detected
    Catalog Number:
    15593031
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|DNA Extraction|General gDNA Purification Reagents & Accessories|Genomic DNA Purification|Miniprep|Plasmid DNA Purification|RNA Extraction|Total RNA Isolation
    Category:
    Lab Reagents and Chemicals
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    Structured Review

    Thermo Fisher ultrapuretm phenol cloroform isoamyl alcohol
    UltraPure Phenol Chloroform Isoamyl Alcohol 25 24 1 v v is used in the purification of nucleic acids This reagent consists of highly pure chloroform isoamyl alcohol and UltraPure Phenol saturated with Tris HCl Using Phenol Chloroform Isoamyl AlcoholWhen mixtures are extracted with Phenol Chloroform Isoamyl Alcohol proteins are denatured and collected in the organic phase or at the interphase while nucleic acids remain in the aqueous phase UltraPure Phenol Chloroform lsoamyl Alcohol contains no preservatives It is packaged under an inert gas in shatter resistant plastic coated amber bottles Performance and quality testingThe appearance of the solution is evaluated at room temperature No RNase or DNase activity is detected
    https://www.bioz.com/result/ultrapuretm phenol cloroform isoamyl alcohol/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ultrapuretm phenol cloroform isoamyl alcohol - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: A Sample Extraction Method for Faster, More Sensitive PCR-Based Detection of Pathogens in Blood Culture
    Article Snippet: .. On separate days, three replicates from each of the sample aliquots were thawed and processed using either the DNeasy blood and tissue kit (69506; Qiagen, Hilden, Germany), phenol-chloroform (15593031; Invitrogen, Carlsbad, CA), or an ABI Automate Express forensic DNA extraction system (4441763; Applied Biosystems, Foster City, CA). ..

    Methylation:

    Article Title: Combined loss of function of two different loci of miR-15/16 drives the pathogenesis of acute myeloid leukemia
    Article Snippet: .. Genomic DNA from cell lines was isolated using phenol:chloroform:isoamyl alcohol (ThermoFisher Scientific) protocol ,and bisulfite conversion was performed using EZ DNA Methylation-Gold Kit (ZymoResearch). .. For target CpG island spanning miR-15a and miR-15b, methylation-specific primers for bisulfite-treated DNA were designed using methprim software.

    Ethanol Precipitation:

    Article Title: Revised role for Hfq bacterial regulator on DNA topology
    Article Snippet: .. Proteins were extracted with ultrapure phenol/chloroform/isoamyl alcohol (25:24:1 v/v, Invitrogen) and ethanol precipitation. ..

    Incubation:

    Article Title: High specificity and tight spatial restriction of self-biotinylation by DNA and RNA G-Quadruplexes complexed in vitro and in vivo with Heme
    Article Snippet: .. To take extra precautions to ensure the purity of the DNA extractions, DNA as extracted using the kit was given further treatments of RNase and proteinase as follows: first, the DNA was incubated with RNase A at 37°C for 1 h, after which it was incubated with Proteinase K at 50°C for 12–14 h. Following the above treatment, the DNA solution was extracted twice with phenol: chloroform: isoamyl alcohol (25:24:1), and then once with chloroform alone. ..

    other:

    Article Title: Genetic and functional diversification of chemosensory pathway receptors in mosquito-borne filarial nematodes
    Article Snippet: Experiments using FBS and NaCl as cues included three biological replicates, and experiments with 3-methyl-1-butanol included two biological replicates.

    Article Title: Unique Epigenetic Programming Distinguishes Regenerative Spermatogonial Stem Cells in the Developing Mouse Testis
    Article Snippet: DNA was extracted by phenol:chloroform:isoamyl alcohol (25:24:1, v/v).

    Isolation:

    Article Title: Combined loss of function of two different loci of miR-15/16 drives the pathogenesis of acute myeloid leukemia
    Article Snippet: .. Genomic DNA from cell lines was isolated using phenol:chloroform:isoamyl alcohol (ThermoFisher Scientific) protocol ,and bisulfite conversion was performed using EZ DNA Methylation-Gold Kit (ZymoResearch). .. For target CpG island spanning miR-15a and miR-15b, methylation-specific primers for bisulfite-treated DNA were designed using methprim software.

    Lysis:

    Article Title: Multiomic Strategies Reveal Diversity and Important Functional Aspects of Human Gut Microbiome
    Article Snippet: .. For efficient lysis of the microbes in the sample, 200 μ L of Phenol/Chloroform/Isoamyl alcohol (25:24:1) (Catalogue #327115000, Acros Organics) was added to the reagents provided with the kit. .. The contents were vortexed for 1-2 min with a table top vortexer and homogenized twice at speed 10 for 5 min with air-cooling using the Bullet Blender Storm Homogenizer (Catalogue # BBY24M, Next Advance Inc).

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  • 99
    Thermo Fisher ultrapuretm phenol chloroform isoamyl alcohol
    Ultrapuretm Phenol Chloroform Isoamyl Alcohol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrapuretm phenol chloroform isoamyl alcohol/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ultrapuretm phenol chloroform isoamyl alcohol - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

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