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Leica Microsystems ultracut e microtome
Ultracut E Microtome, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 89/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ultracut e microtome/product/Leica Microsystems
Average 89 stars, based on 19 article reviews
Price from $9.99 to $1999.99
ultracut e microtome - by Bioz Stars, 2020-08
89/100 stars

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Related Articles

Electron Microscopy:

Article Title: Using Aiptasia as a Model to Study Metabolic Interactions in Cnidarian-Symbiodinium Symbioses
Article Snippet: .. Subsequently, tissues were embedded in SPURR resin and cut into 100 nm sections using an Ultracut E microtome (Leica Microsystems, Germany) and mounted on finder grids for Transmission Electron Microscopy (ProsciTech, Australia). .. NanoSIMS imaging Gold-coated sections were imaged with the NanoSIMS 50 ion probe at the Center for Microscopy, Characterisation and Analysis at the University of Western Australia.

Microscopy:

Article Title: Impaired endothelial barrier function in apolipoprotein M–deficient mice is dependent on sphingosine-1-phosphate receptor 1
Article Snippet: .. Sections, ∼80 nm thick, were cut with an Ultracut E microtome (Reichert-Jung; Leica MicroSystems, Wetzlar, Gemany) and collected on 1-hole copper grids with Formvar-supporting membranes, stained with uranyl acetate and lead citrate, and subsequently examined with a CM 100 transmission electron microscope (Philips, Eindhoven, The Netherlands), operated at an accelerating voltage of 80 kV. .. Digital images were recorded with an Osis Veleta digital slow scan 2k × 2k CCD camera (Olympus, Tokyo Japan) and the ITEM software package (Irvine, CA, USA).

Mouse Assay:

Article Title: Lipin-1 regulates Bnip3–mediated mitophagy in glycolytic muscle
Article Snippet: .. Muscle specimens from mice were fixed in 3.5% glutaraldehyde in 0.1 M cacodylate buffer (pH7.4) at 4°C for 2 h followed by buffer rinse and postfixation in 1% osmium tetroxide for 1 h. Muscle sections were then dehydrated sequentially by 50, 70, 80, 90, and 100% ethanol, infiltrated with graded mixtures of propylene oxides, then embedded in fresh resin and incubated at 60°C for 48 h. Ultrathin sections (60 nm thick) were cut on an Ultracut E Microtome (Reichert-Jung; Leica Microsystems, Wetzlar Germany) with a diamond knife (Diatome, Hatfield, PA, USA), collected on copper grids, and stained with uranyl acetate and lead citrate. .. Electron micrographs were obtained with a Tecnai Biotwin 12-Electron Microscope (Thermo Fisher Scientific, Waltham, MA, USA) equipped with a 4K digital camera (Gatan, Pleasanton, CA, USA).

Light Microscopy:

Article Title: Assessment of Outer Retinal Remodeling in the Hibernating 13-Lined Ground Squirrel
Article Snippet: .. Then, 0.5 μm sections were cut on an Ultracut E microtome (Reichert-Jung/Leica Microsystems) and stained with 1% toluidine blue for light microscopy (LM). .. Contralateral eyes from the six 13-LGSs euthanized for LM histology after OCT were used for immunocytochemistry.

Incubation:

Article Title: Lipin-1 regulates Bnip3–mediated mitophagy in glycolytic muscle
Article Snippet: .. Muscle specimens from mice were fixed in 3.5% glutaraldehyde in 0.1 M cacodylate buffer (pH7.4) at 4°C for 2 h followed by buffer rinse and postfixation in 1% osmium tetroxide for 1 h. Muscle sections were then dehydrated sequentially by 50, 70, 80, 90, and 100% ethanol, infiltrated with graded mixtures of propylene oxides, then embedded in fresh resin and incubated at 60°C for 48 h. Ultrathin sections (60 nm thick) were cut on an Ultracut E Microtome (Reichert-Jung; Leica Microsystems, Wetzlar Germany) with a diamond knife (Diatome, Hatfield, PA, USA), collected on copper grids, and stained with uranyl acetate and lead citrate. .. Electron micrographs were obtained with a Tecnai Biotwin 12-Electron Microscope (Thermo Fisher Scientific, Waltham, MA, USA) equipped with a 4K digital camera (Gatan, Pleasanton, CA, USA).

Transmission Assay:

Article Title: Using Aiptasia as a Model to Study Metabolic Interactions in Cnidarian-Symbiodinium Symbioses
Article Snippet: .. Subsequently, tissues were embedded in SPURR resin and cut into 100 nm sections using an Ultracut E microtome (Leica Microsystems, Germany) and mounted on finder grids for Transmission Electron Microscopy (ProsciTech, Australia). .. NanoSIMS imaging Gold-coated sections were imaged with the NanoSIMS 50 ion probe at the Center for Microscopy, Characterisation and Analysis at the University of Western Australia.

Article Title: Impaired endothelial barrier function in apolipoprotein M–deficient mice is dependent on sphingosine-1-phosphate receptor 1
Article Snippet: .. Sections, ∼80 nm thick, were cut with an Ultracut E microtome (Reichert-Jung; Leica MicroSystems, Wetzlar, Gemany) and collected on 1-hole copper grids with Formvar-supporting membranes, stained with uranyl acetate and lead citrate, and subsequently examined with a CM 100 transmission electron microscope (Philips, Eindhoven, The Netherlands), operated at an accelerating voltage of 80 kV. .. Digital images were recorded with an Osis Veleta digital slow scan 2k × 2k CCD camera (Olympus, Tokyo Japan) and the ITEM software package (Irvine, CA, USA).

Staining:

Article Title: Tryptophan‐rich basic protein ( WRB) mediates insertion of the tail‐anchored protein otoferlin and is required for hair cell exocytosis and hearing
Article Snippet: .. An Ultracut E microtome (Leica Microsystems, Wetzlar, Germany) equipped with a diamond knife (Diatome, Nidau, Switzerland) was used to obtain ultrathin sections (65–75 nm) of the specimen, which were then stained with 4% uranyl acetate (w/v in distilled water) and Reynold's lead citrate. .. Specimens were observed with a JEM 1011 transmission electron microscope (JEOL, Freising, Germany) and micrographs acquired by a Gatan Orius 1200A camera (Gatan GmbH, München, Germany) using the Digital Micrograph software package).

Article Title: Tanshinone IIA ameliorates diabetic cardiomyopathy by inhibiting Grp78 and CHOP expression in STZ-induced diabetes rats
Article Snippet: .. Then, 120 nm-thick sections were cut using a histology diamond knife on an Ultracut E microtome (Leica Microsystems GmbH, Wetzlar, Germany) and stained with 4% uranyl acetate for 20 min and with 0.5% lead citrate for 5 min at room temperature. ..

Article Title: Assessment of Outer Retinal Remodeling in the Hibernating 13-Lined Ground Squirrel
Article Snippet: .. Then, 0.5 μm sections were cut on an Ultracut E microtome (Reichert-Jung/Leica Microsystems) and stained with 1% toluidine blue for light microscopy (LM). .. Contralateral eyes from the six 13-LGSs euthanized for LM histology after OCT were used for immunocytochemistry.

Article Title: Lipin-1 regulates Bnip3–mediated mitophagy in glycolytic muscle
Article Snippet: .. Muscle specimens from mice were fixed in 3.5% glutaraldehyde in 0.1 M cacodylate buffer (pH7.4) at 4°C for 2 h followed by buffer rinse and postfixation in 1% osmium tetroxide for 1 h. Muscle sections were then dehydrated sequentially by 50, 70, 80, 90, and 100% ethanol, infiltrated with graded mixtures of propylene oxides, then embedded in fresh resin and incubated at 60°C for 48 h. Ultrathin sections (60 nm thick) were cut on an Ultracut E Microtome (Reichert-Jung; Leica Microsystems, Wetzlar Germany) with a diamond knife (Diatome, Hatfield, PA, USA), collected on copper grids, and stained with uranyl acetate and lead citrate. .. Electron micrographs were obtained with a Tecnai Biotwin 12-Electron Microscope (Thermo Fisher Scientific, Waltham, MA, USA) equipped with a 4K digital camera (Gatan, Pleasanton, CA, USA).

Article Title: Impaired endothelial barrier function in apolipoprotein M–deficient mice is dependent on sphingosine-1-phosphate receptor 1
Article Snippet: .. Sections, ∼80 nm thick, were cut with an Ultracut E microtome (Reichert-Jung; Leica MicroSystems, Wetzlar, Gemany) and collected on 1-hole copper grids with Formvar-supporting membranes, stained with uranyl acetate and lead citrate, and subsequently examined with a CM 100 transmission electron microscope (Philips, Eindhoven, The Netherlands), operated at an accelerating voltage of 80 kV. .. Digital images were recorded with an Osis Veleta digital slow scan 2k × 2k CCD camera (Olympus, Tokyo Japan) and the ITEM software package (Irvine, CA, USA).

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  • 89
    Leica Microsystems reichert ultracut e microtome
    Reichert Ultracut E Microtome, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 89/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reichert ultracut e microtome/product/Leica Microsystems
    Average 89 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    reichert ultracut e microtome - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    89
    Leica Microsystems ultracut e microtome
    Ultracut E Microtome, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 89/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultracut e microtome/product/Leica Microsystems
    Average 89 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    ultracut e microtome - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

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