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Stratagene ultracompetent e coli xl1 blue cells
Ultracompetent E Coli Xl1 Blue Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ultracompetent e coli xl1 blue cells/product/Stratagene
Average 81 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ultracompetent e coli xl1 blue cells - by Bioz Stars, 2020-04
81/100 stars

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DNA Extraction:

Article Title: Diversity and Seasonal Fluctuations of the Dominant Members of the Bacterial Soil Community in a Wheat Field as Determined by Cultivation and Molecular Methods
Article Snippet: Paragraph title: DNA extraction from soil, PCR amplification and cloning of 16S ribosomal DNA (rDNA). ... Ligation mixes were transformed into ultracompetent Escherichia coli XL1-Blue cells (Stratagene, Cambridge, United Kingdom) according to the manufacturer's instructions.

Clone Assay:

Article Title: Analysis of Fungal Diversity in the Wheat Rhizosphere by Sequencing of Cloned PCR-Amplified Genes Encoding 18S rRNA and Temperature Gradient Gel Electrophoresis
Article Snippet: Paragraph title: Cloning of EF4-EF3 and EF4-fung5 PCR products. ... Ligation mixes were used to transform ultracompetent E. coli XL1-Blue cells (Stratagene, Cambridge, United Kingdom) according to the manufacturer’s instructions.

Article Title: Diversity and Seasonal Fluctuations of the Dominant Members of the Bacterial Soil Community in a Wheat Field as Determined by Cultivation and Molecular Methods
Article Snippet: Paragraph title: DNA extraction from soil, PCR amplification and cloning of 16S ribosomal DNA (rDNA). ... Ligation mixes were transformed into ultracompetent Escherichia coli XL1-Blue cells (Stratagene, Cambridge, United Kingdom) according to the manufacturer's instructions.

Amplification:

Article Title: Analysis of Fungal Diversity in the Wheat Rhizosphere by Sequencing of Cloned PCR-Amplified Genes Encoding 18S rRNA and Temperature Gradient Gel Electrophoresis
Article Snippet: Ligation mixes were used to transform ultracompetent E. coli XL1-Blue cells (Stratagene, Cambridge, United Kingdom) according to the manufacturer’s instructions. .. Subsequently, a quick clone screening procedure based on PCR amplification was performed directly from colony material.

Article Title: Diversity and Seasonal Fluctuations of the Dominant Members of the Bacterial Soil Community in a Wheat Field as Determined by Cultivation and Molecular Methods
Article Snippet: Paragraph title: DNA extraction from soil, PCR amplification and cloning of 16S ribosomal DNA (rDNA). ... Ligation mixes were transformed into ultracompetent Escherichia coli XL1-Blue cells (Stratagene, Cambridge, United Kingdom) according to the manufacturer's instructions.

Agarose Gel Electrophoresis:

Article Title: Diversity and Seasonal Fluctuations of the Dominant Members of the Bacterial Soil Community in a Wheat Field as Determined by Cultivation and Molecular Methods
Article Snippet: PCR-amplified 16S rDNA sequences from the soil microbial community were separated on a 1% agarose gel in Tris-borate-EDTA buffer (89 nM, 89 mM, and 2 mM). .. Ligation mixes were transformed into ultracompetent Escherichia coli XL1-Blue cells (Stratagene, Cambridge, United Kingdom) according to the manufacturer's instructions.

Ligation:

Article Title: Analysis of Fungal Diversity in the Wheat Rhizosphere by Sequencing of Cloned PCR-Amplified Genes Encoding 18S rRNA and Temperature Gradient Gel Electrophoresis
Article Snippet: .. Ligation mixes were used to transform ultracompetent E. coli XL1-Blue cells (Stratagene, Cambridge, United Kingdom) according to the manufacturer’s instructions. .. White colonies were selected, cultured in 2 ml of Luria broth, and stored at −70°C.

Article Title: Diversity and Seasonal Fluctuations of the Dominant Members of the Bacterial Soil Community in a Wheat Field as Determined by Cultivation and Molecular Methods
Article Snippet: .. Ligation mixes were transformed into ultracompetent Escherichia coli XL1-Blue cells (Stratagene, Cambridge, United Kingdom) according to the manufacturer's instructions. .. White colonies on 5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside (X-Gal) medium were picked, and the insert size was determined by performing direct PCR on the cell material using M13 forward and reverse primers.

Cell Culture:

Article Title: Analysis of Fungal Diversity in the Wheat Rhizosphere by Sequencing of Cloned PCR-Amplified Genes Encoding 18S rRNA and Temperature Gradient Gel Electrophoresis
Article Snippet: Ligation mixes were used to transform ultracompetent E. coli XL1-Blue cells (Stratagene, Cambridge, United Kingdom) according to the manufacturer’s instructions. .. White colonies were selected, cultured in 2 ml of Luria broth, and stored at −70°C.

Article Title: Diversity and Seasonal Fluctuations of the Dominant Members of the Bacterial Soil Community in a Wheat Field as Determined by Cultivation and Molecular Methods
Article Snippet: Ligation mixes were transformed into ultracompetent Escherichia coli XL1-Blue cells (Stratagene, Cambridge, United Kingdom) according to the manufacturer's instructions. .. Clones containing an insert with the expected size were cultured in 2 ml of Luria broth and then stored at −70°C.

Polymerase Chain Reaction:

Article Title: Analysis of Fungal Diversity in the Wheat Rhizosphere by Sequencing of Cloned PCR-Amplified Genes Encoding 18S rRNA and Temperature Gradient Gel Electrophoresis
Article Snippet: Paragraph title: Cloning of EF4-EF3 and EF4-fung5 PCR products. ... Ligation mixes were used to transform ultracompetent E. coli XL1-Blue cells (Stratagene, Cambridge, United Kingdom) according to the manufacturer’s instructions.

Article Title: Diversity and Seasonal Fluctuations of the Dominant Members of the Bacterial Soil Community in a Wheat Field as Determined by Cultivation and Molecular Methods
Article Snippet: Paragraph title: DNA extraction from soil, PCR amplification and cloning of 16S ribosomal DNA (rDNA). ... Ligation mixes were transformed into ultracompetent Escherichia coli XL1-Blue cells (Stratagene, Cambridge, United Kingdom) according to the manufacturer's instructions.

Transformation Assay:

Article Title: Diversity and Seasonal Fluctuations of the Dominant Members of the Bacterial Soil Community in a Wheat Field as Determined by Cultivation and Molecular Methods
Article Snippet: .. Ligation mixes were transformed into ultracompetent Escherichia coli XL1-Blue cells (Stratagene, Cambridge, United Kingdom) according to the manufacturer's instructions. .. White colonies on 5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside (X-Gal) medium were picked, and the insert size was determined by performing direct PCR on the cell material using M13 forward and reverse primers.

Denaturing Gradient Gel Electrophoresis:

Article Title: Diversity and Seasonal Fluctuations of the Dominant Members of the Bacterial Soil Community in a Wheat Field as Determined by Cultivation and Molecular Methods
Article Snippet: Ligation mixes were transformed into ultracompetent Escherichia coli XL1-Blue cells (Stratagene, Cambridge, United Kingdom) according to the manufacturer's instructions. .. For DGGE analysis, PCR primers F-968 and R-1401, which were described by Nübel et al. , were used and amplification was performed in a Hybaid PCR Express thermocycler.

Plasmid Preparation:

Article Title: Analysis of Fungal Diversity in the Wheat Rhizosphere by Sequencing of Cloned PCR-Amplified Genes Encoding 18S rRNA and Temperature Gradient Gel Electrophoresis
Article Snippet: DNA fragments were then ligated into the pGEM-T vector, which has 3′ T overhangs to facilitate the cloning of PCR products (Promega, Madison, Wis.). .. Ligation mixes were used to transform ultracompetent E. coli XL1-Blue cells (Stratagene, Cambridge, United Kingdom) according to the manufacturer’s instructions.

Article Title: Diversity and Seasonal Fluctuations of the Dominant Members of the Bacterial Soil Community in a Wheat Field as Determined by Cultivation and Molecular Methods
Article Snippet: DNA fragments were ligated into a pGEM-T vector, which has 3′-T overhangs to facilitate cloning of PCR products (Promega, Madison, Wis.). .. Ligation mixes were transformed into ultracompetent Escherichia coli XL1-Blue cells (Stratagene, Cambridge, United Kingdom) according to the manufacturer's instructions.

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    Stratagene xl1 blue e coli ultracompetent cells
    Xl1 Blue E Coli Ultracompetent Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xl1 blue e coli ultracompetent cells/product/Stratagene
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    xl1 blue e coli ultracompetent cells - by Bioz Stars, 2020-04
    86/100 stars
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