end prep enzyme mix  (New England Biolabs)


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    Structured Review

    New England Biolabs end prep enzyme mix
    End Prep Enzyme Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/end prep enzyme mix/product/New England Biolabs
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    end prep enzyme mix - by Bioz Stars, 2020-02
    93/100 stars

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    Incubation:

    Article Title: Sequencing and phasing cancer mutations in lung cancers using a long-read portable sequencer
    Article Snippet: To perform the end-repair and dA tailing, 7 μl End-Prep buffer and 3 μl End-Prep enzyme mix (NEBNext Ultra II End-Repair/dA-tailing Module, New England Biolabs) were added. .. The template was incubated at 20 °C for 5 min and 65 °C for 5 min and was then purified using Agencourt AMPure XP beads (Beckman Coulter, Inc.).

    Binding Assay:

    Article Title: Sequencing and phasing cancer mutations in lung cancers using a long-read portable sequencer
    Article Snippet: To perform the end-repair and dA tailing, 7 μl End-Prep buffer and 3 μl End-Prep enzyme mix (NEBNext Ultra II End-Repair/dA-tailing Module, New England Biolabs) were added. .. During the ligation, Dynabeads MyOne Streptavidin C1 beads (Life Technologies) were washed twice and suspended with 100 μl Bead Binding Buffer.

    Purification:

    Article Title: Sequencing and phasing cancer mutations in lung cancers using a long-read portable sequencer
    Article Snippet: To perform the end-repair and dA tailing, 7 μl End-Prep buffer and 3 μl End-Prep enzyme mix (NEBNext Ultra II End-Repair/dA-tailing Module, New England Biolabs) were added. .. The template was incubated at 20 °C for 5 min and 65 °C for 5 min and was then purified using Agencourt AMPure XP beads (Beckman Coulter, Inc.).

    Ligation:

    Article Title: Sequencing and phasing cancer mutations in lung cancers using a long-read portable sequencer
    Article Snippet: To perform the end-repair and dA tailing, 7 μl End-Prep buffer and 3 μl End-Prep enzyme mix (NEBNext Ultra II End-Repair/dA-tailing Module, New England Biolabs) were added. .. For the adapter ligation, 8 μl H2 O, 10 μl Adapter Mix, 2 μl HP Adapter and 50 μl Blunt/TA adapter Ligase Master Mix (New England Biolabs) were added to the 30 μl DNA template.

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    New England Biolabs nebnext ultra ii end repair da tailing module
    Nebnext Ultra Ii End Repair Da Tailing Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext ultra ii end repair da tailing module/product/New England Biolabs
    Average 95 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    nebnext ultra ii end repair da tailing module - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    93
    New England Biolabs end prep enzyme mix
    End Prep Enzyme Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/end prep enzyme mix/product/New England Biolabs
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    end prep enzyme mix - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

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