ultimate3000 rslc nano hplc system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ultimate3000 rslc nano hplc system
    Ultimate3000 Rslc Nano Hplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultimate3000 rslc nano hplc system/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ultimate3000 rslc nano hplc system - by Bioz Stars, 2020-05
    99/100 stars

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    Flow Cytometry:

    Article Title: More Is Not Always Better: Evaluation of 1D and 2D-LC-MS/MS Methods for Metaproteomics
    Article Snippet: .. 1D-LC-MS/MS and GeLC-MS/MS For 1D-LC-MS/MS and GeLC-MS/MS analysis, an UltiMateTM 3000 RSLCnano Liquid Chromatograph (Thermo Fisher Scientific) with two 10-port valves in the column oven was used to load the respective amount of peptide mixture ( ) with loading solvent A (2% acetonitrile, 0.05% trifluoroacetic acid) onto a 5 mm, 300 μm ID C18 Acclaim® PepMap100 pre-column (Thermo Fisher Scientific) at a flow rate of 5 μl min−1 . .. The pre-column was then switched into line with the analytical column, which was either a 50 cm × 75 μm analytical EASY-Spray column packed with PepMap RSLC C18, 2 μm material (Thermo Fisher Scientific), heated to 45°C (1D and GeLC), or a 75 cm × 75 μm analytical column with the same packing material (Thermo Fisher Scientific), heated to 60°C (1D-LC only).

    Article Title: Proteomic Analysis of Estrogen-Mediated Signal Transduction in Osteoclasts Formation
    Article Snippet: .. Protein digests were separated by a 120 min gradient elution at a flow rate of 0.3 μ L/min using an UltiMate 3000 RSLCnano System (Thermo Scientific), followed by analysis using a directly interfaced Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Scientific). .. The analytical column was a homemade fused silica capillary column (75 μ m, 150 mm, Upchurch) packed with C18 resin (300 Å, 5 μ m, Varian).

    Mass Spectrometry:

    Article Title: Proteomic Analysis of Estrogen-Mediated Signal Transduction in Osteoclasts Formation
    Article Snippet: .. Protein digests were separated by a 120 min gradient elution at a flow rate of 0.3 μ L/min using an UltiMate 3000 RSLCnano System (Thermo Scientific), followed by analysis using a directly interfaced Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Scientific). .. The analytical column was a homemade fused silica capillary column (75 μ m, 150 mm, Upchurch) packed with C18 resin (300 Å, 5 μ m, Varian).

    Article Title: Altered Levels of Visinin-Like Protein 1 Correspond to Regional Neuronal Loss in Alzheimer Disease and Frontotemporal Lobar Degeneration
    Article Snippet: .. In brief, peptide peaks were detected using a TSQ Quantiva triple stage quadrupole mass spectrometer (Thermo Scientific) with an UltiMate 3000 Nano LC Systems (Thermo Scientific). .. Two μl (∼1 μg protein) of sample was loaded/desalted on a PepMap100 Nano-Trap column (Thermo Scientific) at 8 μl/minute for 2 minutes and separated on a Reprosil-pur 3-µm PicoChip column (New Objective, Woburn, MA) at 400 nl/minute over a 20-minute gradient from 2%–35% mobile phase B (Acetonitrile containing 0.1% formic acid).

    Article Title: Role of Mac-1 integrin in generation of extracellular vesicles with antibacterial capacity from neutrophilic granulocytes
    Article Snippet: .. The resulting peptides were desalted using Pierce™ C18 spin columns (Thermo Fisher Scientific, Waltham, MA) and analysed on a Dionex Ultimate 3000 RSLCnano system (Thermo Fisher Scientific, Waltham, MA) coupled to a Bruker Maxis II Q-TOF mass spectrometer (Bruker, Bremen, Germany) equipped with a CaptiveSpraynanoBooster ionization source. ..

    Article Title: Results supporting the concept of the oxidant-mediated protein amino acid conversion, a naturally occurring protein engineering process, in human cells
    Article Snippet: .. Nanospray LC/MS/MS analysis The tryptic peptides mixture from each sample was analyzed using a Thermo Scientific Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Electron, Bremen, Germany) equipped with a Thermo Dionex UltiMate 3000 RSLCnano System (Thermo Dionex, Sunnyvale, CA, USA). .. Tryptic peptide samples were loaded onto a peptide trap cartridge at a flow rate of 5 μl/min.

    Article Title: In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
    Article Snippet: .. LC-ESI-MS/MS Q-Exactive Online LC-MS was performed using a Dionex UltiMate 3000 RSLCnano System coupled to a Q-Exactive mass spectrometer (Thermo Scientific). .. Each plate well was dissolved in 20 μl solvent A and 10 μl were injected.

    Article Title: Multi-lectin Affinity Chromatography and Quantitative Proteomic Analysis Reveal Differential Glycoform Levels between Prostate Cancer and Benign Prostatic Hyperplasia Sera
    Article Snippet: .. Tryptic peptides were analyzed by LC-MS/MS on an Ultimate 3000 RSLCnano system (Dionex) coupled to an Orbitrap Elite mass spectrometer (Thermo Fischer Scientific) with a nanospray ion source. .. Fifteen µL of sample (approximately 5–10 µg of peptides) was loaded onto a C18 trap column for brief desalting and concentration, then separated on a 25 cm C18 analytical column (Picofrit 75 µm ID, New Objective, packed with MagicC18 AQ resin) over a 140 minute, multi-step gradient of increasing organic phase.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
    Article Snippet: .. LC-ESI-MS/MS Q-Exactive Online LC-MS was performed using a Dionex UltiMate 3000 RSLCnano System coupled to a Q-Exactive mass spectrometer (Thermo Scientific). .. Each plate well was dissolved in 20 μl solvent A and 10 μl were injected.

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  • 99
    Thermo Fisher ultimate3000 rslc nano hplc system
    Ultimate3000 Rslc Nano Hplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultimate3000 rslc nano hplc system/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ultimate3000 rslc nano hplc system - by Bioz Stars, 2020-05
    99/100 stars
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