ultimate3000 nano hplc system  (Thermo Fisher)


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    Name:
    UltiMate 3000 RSLCnano LC System
    Description:
    Get outstanding performance and workflow versatility using the Thermo Scientific UltiMate 3000 RSLCnano system This low flow liquid chromatography system has a wide flow pressure footprint with nano capillary and micro flow options Our Thermo Scientific ProFlow technology improves nano flow rate control and results in high retention time precision and higher quality data Achieve high sensitivity with our nano flow solution or achieve high sensitivity and high throughput with robust capillary and microflow solutions Pre concentrate your sample on a trapping column set up two dimensional workflows with the additional micro flow ternary pump or tailor it to your needs with our dedicated application kits
    Catalog Number:
    5200.0355
    Price:
    None
    Applications:
    Industrial & Applied Science|Industrial Chromatography
    Category:
    Instruments and Equipment
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    Structured Review

    Thermo Fisher ultimate3000 nano hplc system
    LC-MS/MS retinal proteome analysis in R347 versus wt mice. Whole retina protein (n = 4) and retinal membrane protein (n = 4) extracts were prepared from one month-old R347 and wt mice. LC-MS/MS analysis was performed on an <t>Ultimate3000</t> <t>nano</t> <t>HPLC</t> system coupled to a LTQ OrbitrapXL mass spectrometer. ( a ) Representative LC-MS/MS profile detected in the retinal samples. ( b ) Distribution of the 1895 identified protein IDs, which were mapped to 1446 gene IDs. 1337 gene IDs were present in the GO database (GO); GO: M refers to entries with membrane in their GO search terms. ( c ) Volcano plot representation of the identified protein IDs in R347 versus wt retinas. X-axis indicates difference in expression level on a log2 scale, whereas the y-axis represents corresponding p-values (Student’s t-Test) on a negative log scale. Red lines indicate 0.5-fold and 2-fold differences in protein expression and significance level of p = 0.05, respectively. Scaling was limited to -6 and 6 on the horizontal axis and 4 on the vertical axis. Entries outside the limiting values were set to the corresponding limiting values.
    Get outstanding performance and workflow versatility using the Thermo Scientific UltiMate 3000 RSLCnano system This low flow liquid chromatography system has a wide flow pressure footprint with nano capillary and micro flow options Our Thermo Scientific ProFlow technology improves nano flow rate control and results in high retention time precision and higher quality data Achieve high sensitivity with our nano flow solution or achieve high sensitivity and high throughput with robust capillary and microflow solutions Pre concentrate your sample on a trapping column set up two dimensional workflows with the additional micro flow ternary pump or tailor it to your needs with our dedicated application kits
    https://www.bioz.com/result/ultimate3000 nano hplc system/product/Thermo Fisher
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    ultimate3000 nano hplc system - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "microRNA regulatory circuits in a mouse model of inherited retinal degeneration"

    Article Title: microRNA regulatory circuits in a mouse model of inherited retinal degeneration

    Journal: Scientific Reports

    doi: 10.1038/srep31431

    LC-MS/MS retinal proteome analysis in R347 versus wt mice. Whole retina protein (n = 4) and retinal membrane protein (n = 4) extracts were prepared from one month-old R347 and wt mice. LC-MS/MS analysis was performed on an Ultimate3000 nano HPLC system coupled to a LTQ OrbitrapXL mass spectrometer. ( a ) Representative LC-MS/MS profile detected in the retinal samples. ( b ) Distribution of the 1895 identified protein IDs, which were mapped to 1446 gene IDs. 1337 gene IDs were present in the GO database (GO); GO: M refers to entries with membrane in their GO search terms. ( c ) Volcano plot representation of the identified protein IDs in R347 versus wt retinas. X-axis indicates difference in expression level on a log2 scale, whereas the y-axis represents corresponding p-values (Student’s t-Test) on a negative log scale. Red lines indicate 0.5-fold and 2-fold differences in protein expression and significance level of p = 0.05, respectively. Scaling was limited to -6 and 6 on the horizontal axis and 4 on the vertical axis. Entries outside the limiting values were set to the corresponding limiting values.
    Figure Legend Snippet: LC-MS/MS retinal proteome analysis in R347 versus wt mice. Whole retina protein (n = 4) and retinal membrane protein (n = 4) extracts were prepared from one month-old R347 and wt mice. LC-MS/MS analysis was performed on an Ultimate3000 nano HPLC system coupled to a LTQ OrbitrapXL mass spectrometer. ( a ) Representative LC-MS/MS profile detected in the retinal samples. ( b ) Distribution of the 1895 identified protein IDs, which were mapped to 1446 gene IDs. 1337 gene IDs were present in the GO database (GO); GO: M refers to entries with membrane in their GO search terms. ( c ) Volcano plot representation of the identified protein IDs in R347 versus wt retinas. X-axis indicates difference in expression level on a log2 scale, whereas the y-axis represents corresponding p-values (Student’s t-Test) on a negative log scale. Red lines indicate 0.5-fold and 2-fold differences in protein expression and significance level of p = 0.05, respectively. Scaling was limited to -6 and 6 on the horizontal axis and 4 on the vertical axis. Entries outside the limiting values were set to the corresponding limiting values.

    Techniques Used: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Mouse Assay, High Performance Liquid Chromatography, Expressing

    Related Articles

    High Performance Liquid Chromatography:

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    Article Snippet: .. The chiral nanoHPLC column was installed on a Dionex Ultimate 3000 RSLCnano HPLC system coupled to an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific, San Jose, CA) for the separation and quantitation of the NNN enantiomers. .. The samples were first loaded onto the nanoHPLC column through a 5 μL injection loop with 5% CH3 OH in 5 mM NH4 OAc buffer at a flow rate of 1 μL/min for 5.5 min.

    Article Title: Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions
    Article Snippet: .. The eluted peptides were dried and resuspended in 5% acetonitrile and 0.1% formic acid solution, and were further processed for LC-MS in an online UltiMate 3000 RSLCnano HPLC system (ThermoFisher Scientific) coupled online to the Q-Exactive-HF. ..

    Quantitation Assay:

    Article Title: Analysis of N′-Nitrosonornicotine Enantiomers in Human Urine by Chiral Stationary Phase Liquid Chromatography-Nanoelectrospray Ionization-High Resolution Tandem Mass Spectrometry
    Article Snippet: .. The chiral nanoHPLC column was installed on a Dionex Ultimate 3000 RSLCnano HPLC system coupled to an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific, San Jose, CA) for the separation and quantitation of the NNN enantiomers. .. The samples were first loaded onto the nanoHPLC column through a 5 μL injection loop with 5% CH3 OH in 5 mM NH4 OAc buffer at a flow rate of 1 μL/min for 5.5 min.

    Liquid Chromatography:

    Article Title: Proteomic Characterization of the Neural Ectoderm Fated Cell Clones in the Xenopus laevis Embryo by High-Resolution Mass Spectrometry
    Article Snippet: .. Separation used a nanoflow liquid chromatography system (Dionex Ultimate 3000 RSLCnano, Thermo) providing a 120 min gradient mixture from Buffer A (2% ACN, 0.1% FA) and Buffer B (100% ACN, 0.1% FA). ..

    Article Title: Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 2. Label-free relative quantitative proteomics mastitis: 2. Label-free relative quantitative proteomics †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6mb00290kClick here for additional
    Article Snippet: .. 2.1.4 On-line liquid chromatography and tandem mass spectrometry For on-line reversed-phase liquid chromatography and mass spectrometry (LC-MS), a Dionex UltiMate 3000 RSLCnano (liquid chromatography) system coupled to a Thermo Scientific Orbitrap Elite mass spectrometer was used. .. A stainless steel Nano-Trap column with 300 μm inside diameter, 5 mm length, particle size 5 μm and pore size 10 nm, packed with stationary phase Acclaim PepMap C18 (Thermo Scientific, part no. 160454) and a resolving Nano LC column with 75 μm inside diameter, 15 cm length, particle size 2 μm and pore diameter 10 nm with stationary phase Acclaim PepMap RSLC C18 (Thermo Fisher Scientific, part no. 164534) were used in the HPLC.

    Mass Spectrometry:

    Article Title: The Interactome of EBV LMP1 Evaluated by Proximity-Based BioID Approach
    Article Snippet: .. Samples from the replicates experiments were analyzed with an externally calibrated Thermo Q Exactive HF (high-resolution electrospray tandem mass spectrometer) in conjunction with Dionex UltiMate3000 RSLCnano System at the FSU Translational Science Laboratory. ..

    Article Title: Analysis of N′-Nitrosonornicotine Enantiomers in Human Urine by Chiral Stationary Phase Liquid Chromatography-Nanoelectrospray Ionization-High Resolution Tandem Mass Spectrometry
    Article Snippet: .. The chiral nanoHPLC column was installed on a Dionex Ultimate 3000 RSLCnano HPLC system coupled to an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific, San Jose, CA) for the separation and quantitation of the NNN enantiomers. .. The samples were first loaded onto the nanoHPLC column through a 5 μL injection loop with 5% CH3 OH in 5 mM NH4 OAc buffer at a flow rate of 1 μL/min for 5.5 min.

    Article Title: Separating Golgi Proteins from Cis to Trans Reveals Underlying Properties of Cisternal Localization [OPEN]
    Article Snippet: .. All MS runs were performed on an Orbitrap Fusion Lumos Tribrid instrument coupled to a Dionex Ultimate 3000 RSLCnano system (Thermo Fisher Scientific) with parameters from . .. Raw files were processed with Proteome Discoverer v1.4 (Thermo Fisher Scientific) using the MASCOT server v2.3.02 (Matrix Science), searched against the Arabidopsis proteome (canonical sequences, downloaded on April 2, 2017).

    Article Title: Mitochondrial biogenesis is required for the anchorage-independent survival and propagation of stem-like cancer cells
    Article Snippet: .. All LC-MS/MS analyses were performed on an LTQ Orbitrap XL mass spectrometer (Thermo Scientific, San Jose, CA) coupled to an Ultimate 3000 RSLCnano system (Thermo Scientific, formerly Dionex, The Netherlands). .. Xcalibur raw data files acquired on the LTQ-Orbitrap XL were directly imported into Progenesis LCMS software (Waters Corp., Milford, MA, formerly Non-linear dynamics, Newcastle upon Tyne, UK) for peak detection and alignment.

    Article Title: Results supporting the concept of the oxidant-mediated protein amino acid conversion, a naturally occurring protein engineering process, in human cells
    Article Snippet: .. Nanospray LC/MS/MS analysis The tryptic peptides mixture from each sample was analyzed using a Thermo Scientific Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Electron, Bremen, Germany) equipped with a Thermo Dionex UltiMate 3000 RSLCnano System (Thermo Dionex, Sunnyvale, CA, USA). .. Tryptic peptide samples were loaded onto a peptide trap cartridge at a flow rate of 5 μl/min.

    Article Title: Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 2. Label-free relative quantitative proteomics mastitis: 2. Label-free relative quantitative proteomics †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6mb00290kClick here for additional
    Article Snippet: .. 2.1.4 On-line liquid chromatography and tandem mass spectrometry For on-line reversed-phase liquid chromatography and mass spectrometry (LC-MS), a Dionex UltiMate 3000 RSLCnano (liquid chromatography) system coupled to a Thermo Scientific Orbitrap Elite mass spectrometer was used. .. A stainless steel Nano-Trap column with 300 μm inside diameter, 5 mm length, particle size 5 μm and pore size 10 nm, packed with stationary phase Acclaim PepMap C18 (Thermo Scientific, part no. 160454) and a resolving Nano LC column with 75 μm inside diameter, 15 cm length, particle size 2 μm and pore diameter 10 nm with stationary phase Acclaim PepMap RSLC C18 (Thermo Fisher Scientific, part no. 164534) were used in the HPLC.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions
    Article Snippet: .. The eluted peptides were dried and resuspended in 5% acetonitrile and 0.1% formic acid solution, and were further processed for LC-MS in an online UltiMate 3000 RSLCnano HPLC system (ThermoFisher Scientific) coupled online to the Q-Exactive-HF. ..

    Article Title: Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 2. Label-free relative quantitative proteomics mastitis: 2. Label-free relative quantitative proteomics †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6mb00290kClick here for additional
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