ultimate 3000 uhplc system  (Thermo Fisher)


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    Name:
    UltiMate 3000 Standard Binary System
    Description:
    Get stable retention times and maximum information capture from high throughput routine applications with the Thermo Scientific UltiMate 3000 Standard Binary System The fast gradient response excellent flow stability and a broad choice of high data rate detection technologies ensure reliable results from every sample making the UltiMate 3000 Standard Binary System an ideal choice for demanding routine environments or high throughput research and development laboratories
    Catalog Number:
    iqlaaagabhfapbmbex
    Price:
    None
    Applications:
    Industrial & Applied Science|Industrial Chromatography
    Category:
    Instruments and Equipment
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    Structured Review

    Thermo Fisher ultimate 3000 uhplc system
    ( a ) Photograph of an Indian saw scaled viper ( Echis carinatus ). Photograph courtesy of Romulus Whitaker, Nov, 2010. ( b ) Fractionation of ECV (2.0 mg dry weight) on a Shodex KW- 803 gel filtration column coupled to Dionex <t>Ultimate</t> 3000 <t>UHPLC</t> system (Thermo Fisher Scientific, USA). The flow rate was maintained at 10 ml/h at room temperature (~23 °C) and fractions of 0.25 ml were collected. ( c ) SDS-PAGE analysis of crude ECV. Lane 1, 2 and 3 represents crude ECV (non-reduced), protein molecular markers and crude ECV (reduced) respectively.
    Get stable retention times and maximum information capture from high throughput routine applications with the Thermo Scientific UltiMate 3000 Standard Binary System The fast gradient response excellent flow stability and a broad choice of high data rate detection technologies ensure reliable results from every sample making the UltiMate 3000 Standard Binary System an ideal choice for demanding routine environments or high throughput research and development laboratories
    https://www.bioz.com/result/ultimate 3000 uhplc system/product/Thermo Fisher
    Average 99 stars, based on 73 article reviews
    Price from $9.99 to $1999.99
    ultimate 3000 uhplc system - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Proteomics and antivenomics of Echis carinatus carinatus venom: Correlation with pharmacological properties and pathophysiology of envenomation"

    Article Title: Proteomics and antivenomics of Echis carinatus carinatus venom: Correlation with pharmacological properties and pathophysiology of envenomation

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-17227-y

    ( a ) Photograph of an Indian saw scaled viper ( Echis carinatus ). Photograph courtesy of Romulus Whitaker, Nov, 2010. ( b ) Fractionation of ECV (2.0 mg dry weight) on a Shodex KW- 803 gel filtration column coupled to Dionex Ultimate 3000 UHPLC system (Thermo Fisher Scientific, USA). The flow rate was maintained at 10 ml/h at room temperature (~23 °C) and fractions of 0.25 ml were collected. ( c ) SDS-PAGE analysis of crude ECV. Lane 1, 2 and 3 represents crude ECV (non-reduced), protein molecular markers and crude ECV (reduced) respectively.
    Figure Legend Snippet: ( a ) Photograph of an Indian saw scaled viper ( Echis carinatus ). Photograph courtesy of Romulus Whitaker, Nov, 2010. ( b ) Fractionation of ECV (2.0 mg dry weight) on a Shodex KW- 803 gel filtration column coupled to Dionex Ultimate 3000 UHPLC system (Thermo Fisher Scientific, USA). The flow rate was maintained at 10 ml/h at room temperature (~23 °C) and fractions of 0.25 ml were collected. ( c ) SDS-PAGE analysis of crude ECV. Lane 1, 2 and 3 represents crude ECV (non-reduced), protein molecular markers and crude ECV (reduced) respectively.

    Techniques Used: Fractionation, Filtration, Flow Cytometry, SDS Page

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Non-viral adeno-associated virus-based platform for stable expression of antibody combination therapeutics
    Article Snippet: .. CIEX chromatography was performed on an Ultimate3000 HPLC system, and Chromeleon software was used for HPLC control and data analysis (both system and software from Thermo Scientific). .. The cation exchange column (TSKgel-CM STAT, 4.6 × 150 mm, TOSOH Bioscience, Japan) was equilibrated with 20 mM MES, 50 mM NaCl and antibodies were eluted by a linear gradient of NaCl from 50 to 500 mM.

    Article Title: Do Plant Cyclotides Have Potential As Immunosuppressant Peptides?
    Article Snippet: .. Crude, ZipTip prepared, or digested plant extracts (C18 prepurified O. affinis extract, see above) were analyzed by nano LC-MS or LC-MS/MS on an Ultimate 3000 nano HPLC system controlled by Chromeleon 6.8 software (Dionex, Amsterdam, The Netherlands). .. For LC analysis, 1–5 μ L samples of O. affinis extract were injected, preconcentrated using Dionex PepMap C18 cartridges (300 μ m × 5 mm, 5 μ m, 100 Å), and separated by nano-RP-HPLC prior to online MS analysis using a Dionex Acclaim PepMap C18 column (150 mm × 75 μ m, 3 μ m, 100 Å; 300 nL/min).

    Article Title: Preparation, Characterization, and Stability Evaluation of Taste-Masking Lacosamide Microparticles
    Article Snippet: .. Analysis was performed using an Ultimate 3000® series HPLC system (Thermo scientific, Waltham, MA, USA). .. The determination was performed on Inertsil 4.6 mm × 26 cm packing L7 column (GL sciences Tokyo, Japan).

    Article Title: Supply of Methionine During Late-Pregnancy Alters Fecal Microbiota and Metabolome in Neonatal Dairy Calves Without Changes in Daily Feed Intake
    Article Snippet: .. The Dionex Ultimate 3000 series HPLC system (Thermo, Germering, Germany) used had a degasser, an autosampler and a binary pump. .. The LC separation was performed on a Phenomenex Kinetex C18 column (4.6 × 100 mm, 2.6 μm) with mobile phase A (H2 O with 0.1% formic acid) and mobile phase B (acetonitrile with 0.1% formic acid).

    Article Title: Metabolic Pathway of Topramezone in Multiple-Resistant Waterhemp (Amaranthus tuberculatus) Differs From Naturally Tolerant Maize
    Article Snippet: .. Samples for high-resolution LC-MS were analyzed using a Dionex Ultimate 3000 series HPLC system (Thermo, Germering, Germany) and Q-Exactive MS system (Thermo, Bremen, Germany) in the Metabolomics Laboratory of the Roy J. .. Carver Biotechnology Center, University of Illinois at Urbana-Champaign.

    Filtration:

    Article Title: Proteomics and antivenomics of Echis carinatus carinatus venom: Correlation with pharmacological properties and pathophysiology of envenomation
    Article Snippet: .. Then 0.1 ml filtrate containing 1.5 mg of protein was fractionated on a Shodex KW-803 gel filtration column (8 × 300 mm, 5 µm) equipped with Dionex Ultimate 3000 UHPLC system (Thermo Fisher Scientific, USA). .. The column was pre-equilibrated with the above buffer and the flow rate was adjusted to 15 ml/h at room temperature (~23 °C).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Do Plant Cyclotides Have Potential As Immunosuppressant Peptides?
    Article Snippet: .. Crude, ZipTip prepared, or digested plant extracts (C18 prepurified O. affinis extract, see above) were analyzed by nano LC-MS or LC-MS/MS on an Ultimate 3000 nano HPLC system controlled by Chromeleon 6.8 software (Dionex, Amsterdam, The Netherlands). .. For LC analysis, 1–5 μ L samples of O. affinis extract were injected, preconcentrated using Dionex PepMap C18 cartridges (300 μ m × 5 mm, 5 μ m, 100 Å), and separated by nano-RP-HPLC prior to online MS analysis using a Dionex Acclaim PepMap C18 column (150 mm × 75 μ m, 3 μ m, 100 Å; 300 nL/min).

    Article Title: Metabolic Pathway of Topramezone in Multiple-Resistant Waterhemp (Amaranthus tuberculatus) Differs From Naturally Tolerant Maize
    Article Snippet: .. Samples for high-resolution LC-MS were analyzed using a Dionex Ultimate 3000 series HPLC system (Thermo, Germering, Germany) and Q-Exactive MS system (Thermo, Bremen, Germany) in the Metabolomics Laboratory of the Roy J. .. Carver Biotechnology Center, University of Illinois at Urbana-Champaign.

    Chromatography:

    Article Title: Non-viral adeno-associated virus-based platform for stable expression of antibody combination therapeutics
    Article Snippet: .. CIEX chromatography was performed on an Ultimate3000 HPLC system, and Chromeleon software was used for HPLC control and data analysis (both system and software from Thermo Scientific). .. The cation exchange column (TSKgel-CM STAT, 4.6 × 150 mm, TOSOH Bioscience, Japan) was equilibrated with 20 mM MES, 50 mM NaCl and antibodies were eluted by a linear gradient of NaCl from 50 to 500 mM.

    Immunoprecipitation:

    Article Title: Novel Insights into the PKCβ-dependent Regulation of the Oxidoreductase p66Shc *
    Article Snippet: .. Immunoprecipitated proteins were digested on beads with trypsin and analyzed using an UltiMate 3000 nano-HPLC system coupled via an electrospray ionization interface to an LTQ Velos mass spectrometer (Thermo Scientific). .. HPLC conditions were the same as with the in vitro kinase assay, except the particle size of the column material, which was 5-μm reverse-phase C18.

    Mass Spectrometry:

    Article Title: Novel Insights into the PKCβ-dependent Regulation of the Oxidoreductase p66Shc *
    Article Snippet: .. Immunoprecipitated proteins were digested on beads with trypsin and analyzed using an UltiMate 3000 nano-HPLC system coupled via an electrospray ionization interface to an LTQ Velos mass spectrometer (Thermo Scientific). .. HPLC conditions were the same as with the in vitro kinase assay, except the particle size of the column material, which was 5-μm reverse-phase C18.

    Article Title: Metabolic Pathway of Topramezone in Multiple-Resistant Waterhemp (Amaranthus tuberculatus) Differs From Naturally Tolerant Maize
    Article Snippet: .. Samples for high-resolution LC-MS were analyzed using a Dionex Ultimate 3000 series HPLC system (Thermo, Germering, Germany) and Q-Exactive MS system (Thermo, Bremen, Germany) in the Metabolomics Laboratory of the Roy J. .. Carver Biotechnology Center, University of Illinois at Urbana-Champaign.

    Article Title: Asymmetric arginine dimethylation of RelA provides a repressive mark to modulate TNFα/NF-κB response
    Article Snippet: .. Samples were analyzed with an UltiMate 3000 nano-HPLC system coupled to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific) equipped with a Nanospray Flex ionization source. ..

    Software:

    Article Title: Non-viral adeno-associated virus-based platform for stable expression of antibody combination therapeutics
    Article Snippet: .. CIEX chromatography was performed on an Ultimate3000 HPLC system, and Chromeleon software was used for HPLC control and data analysis (both system and software from Thermo Scientific). .. The cation exchange column (TSKgel-CM STAT, 4.6 × 150 mm, TOSOH Bioscience, Japan) was equilibrated with 20 mM MES, 50 mM NaCl and antibodies were eluted by a linear gradient of NaCl from 50 to 500 mM.

    Article Title: Do Plant Cyclotides Have Potential As Immunosuppressant Peptides?
    Article Snippet: .. Crude, ZipTip prepared, or digested plant extracts (C18 prepurified O. affinis extract, see above) were analyzed by nano LC-MS or LC-MS/MS on an Ultimate 3000 nano HPLC system controlled by Chromeleon 6.8 software (Dionex, Amsterdam, The Netherlands). .. For LC analysis, 1–5 μ L samples of O. affinis extract were injected, preconcentrated using Dionex PepMap C18 cartridges (300 μ m × 5 mm, 5 μ m, 100 Å), and separated by nano-RP-HPLC prior to online MS analysis using a Dionex Acclaim PepMap C18 column (150 mm × 75 μ m, 3 μ m, 100 Å; 300 nL/min).

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  • 84
    Thermo Fisher ultimateã¢â„⢠3000 uhplc system
    Ultimateã¢â„⢠3000 Uhplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultimateã¢â„⢠3000 uhplc system/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ultimateã¢â„⢠3000 uhplc system - by Bioz Stars, 2020-07
    84/100 stars
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