ultimate 3000 standard high pressure liquid chromatographic instrument  (Thermo Fisher)


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    Name:
    OP PQ Standards for UltiMate 3000 Systems
    Description:
    Choose Thermo Scientific Dionex UltiMate HPLC Qualification Kits for system validation of UltiMate 3000 LC and UHPLC systems
    Catalog Number:
    3323.0010
    Price:
    None
    Applications:
    Industrial & Applied Science|Industrial Chromatography
    Category:
    Instruments and Equipment
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    Thermo Fisher ultimate 3000 standard high pressure liquid chromatographic instrument
    Choose Thermo Scientific Dionex UltiMate HPLC Qualification Kits for system validation of UltiMate 3000 LC and UHPLC systems
    https://www.bioz.com/result/ultimate 3000 standard high pressure liquid chromatographic instrument/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ultimate 3000 standard high pressure liquid chromatographic instrument - by Bioz Stars, 2020-09
    94/100 stars

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    High Performance Liquid Chromatography:

    Article Title: Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection
    Article Snippet: .. HPLC Instrumentation and Chromatographic Conditions An UltiMate® 3000 standard high pressure liquid chromatographic instrument (Dionex, Germany) composed of an UltiMate 3000 quaternary gradient pump, an ASI-100 autosampler, a TCC-100 thermostat column oven, and an ultraviolet detector (DAD) was employed in the study. .. Data acquisition was carried out using Chromeleon® software.

    Flow Cytometry:

    Article Title: Gastropods and Insects Prefer Different Solanum dulcamara Chemotypes
    Article Snippet: .. Two sets of diluted crude extracts (1:5 and 1:50) were analysed with an UltiMate™ 3000 Standard Ultra-High-Pressure Liquid Chromatography system (UHPLC, Thermo Scientific) equipped with an Acclaim® Rapid Separation Liquid Chromatography (RSLC) 120 column (150 × 2.1 mm, particle size 2.2 μm, ThermoFisher Scientific) using the following gradient at a flow rate of 0.4 ml/min: 0–2 min isocratic 95% A (water/ formic acid 99.95/0.05 (v/v %)), 5% B (acetonitrile/formic acid 99.95/0.05 (v/v %)); 2–15 min, linear from 5 to 40% B; 15–20 min, linear from 40 to 95% B; 20–22 min, isocratic 95% B; 22–25 min, linear from 95 to 5% B; 25–30 min, isocratic 5% B. .. Compounds were detected with a maXis impact –quadrupole time-of-flight mass spectrometer (qToF-MS, Bruker Daltonics) applying the following conditions in positive mode: scan range 50–1400 m /z ; acquisition rate 3 Hz; end plate offset 500 V; capillary voltage 3500 V; nebulizer pressure 2 bar, dry gas 10 L min−1 , dry temperature 220 °C.

    Liquid Chromatography:

    Article Title: Gastropods and Insects Prefer Different Solanum dulcamara Chemotypes
    Article Snippet: .. Two sets of diluted crude extracts (1:5 and 1:50) were analysed with an UltiMate™ 3000 Standard Ultra-High-Pressure Liquid Chromatography system (UHPLC, Thermo Scientific) equipped with an Acclaim® Rapid Separation Liquid Chromatography (RSLC) 120 column (150 × 2.1 mm, particle size 2.2 μm, ThermoFisher Scientific) using the following gradient at a flow rate of 0.4 ml/min: 0–2 min isocratic 95% A (water/ formic acid 99.95/0.05 (v/v %)), 5% B (acetonitrile/formic acid 99.95/0.05 (v/v %)); 2–15 min, linear from 5 to 40% B; 15–20 min, linear from 40 to 95% B; 20–22 min, isocratic 95% B; 22–25 min, linear from 95 to 5% B; 25–30 min, isocratic 5% B. .. Compounds were detected with a maXis impact –quadrupole time-of-flight mass spectrometer (qToF-MS, Bruker Daltonics) applying the following conditions in positive mode: scan range 50–1400 m /z ; acquisition rate 3 Hz; end plate offset 500 V; capillary voltage 3500 V; nebulizer pressure 2 bar, dry gas 10 L min−1 , dry temperature 220 °C.

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  • 99
    Thermo Fisher ultimate 3000 nano hplc system
    Ser 139 , Thr 206 , and Ser 213 are PKCβ-regulatory sites. A , for mass spectrometry, an in vitro kinase assay was performed with recombinant kinases and p66 as substrate and subjected to SDS-PAGE. The gel was stained with Coomassie or immunoblotted for p66 Ser 36 . B , the protein bands were excised from Coomassie-stained gel and digested with trypsin or Lys C. Both Tryptic ( C ) and Lys C ( D ) digests were analyzed by <t>nano-HPLC</t> coupled via an electrospray ionization interface to a Q Exactive HF mass spectrometer. Data analysis and peak area calculation were performed using Proteome Discoverer 1.4.1.14.
    Ultimate 3000 Nano Hplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 216 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultimate 3000 nano hplc system/product/Thermo Fisher
    Average 99 stars, based on 216 article reviews
    Price from $9.99 to $1999.99
    ultimate 3000 nano hplc system - by Bioz Stars, 2020-09
    99/100 stars
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    Ser 139 , Thr 206 , and Ser 213 are PKCβ-regulatory sites. A , for mass spectrometry, an in vitro kinase assay was performed with recombinant kinases and p66 as substrate and subjected to SDS-PAGE. The gel was stained with Coomassie or immunoblotted for p66 Ser 36 . B , the protein bands were excised from Coomassie-stained gel and digested with trypsin or Lys C. Both Tryptic ( C ) and Lys C ( D ) digests were analyzed by nano-HPLC coupled via an electrospray ionization interface to a Q Exactive HF mass spectrometer. Data analysis and peak area calculation were performed using Proteome Discoverer 1.4.1.14.

    Journal: The Journal of Biological Chemistry

    Article Title: Novel Insights into the PKCβ-dependent Regulation of the Oxidoreductase p66Shc *

    doi: 10.1074/jbc.M116.752766

    Figure Lengend Snippet: Ser 139 , Thr 206 , and Ser 213 are PKCβ-regulatory sites. A , for mass spectrometry, an in vitro kinase assay was performed with recombinant kinases and p66 as substrate and subjected to SDS-PAGE. The gel was stained with Coomassie or immunoblotted for p66 Ser 36 . B , the protein bands were excised from Coomassie-stained gel and digested with trypsin or Lys C. Both Tryptic ( C ) and Lys C ( D ) digests were analyzed by nano-HPLC coupled via an electrospray ionization interface to a Q Exactive HF mass spectrometer. Data analysis and peak area calculation were performed using Proteome Discoverer 1.4.1.14.

    Article Snippet: Immunoprecipitated proteins were digested on beads with trypsin and analyzed using an UltiMate 3000 nano-HPLC system coupled via an electrospray ionization interface to an LTQ Velos mass spectrometer (Thermo Scientific).

    Techniques: Mass Spectrometry, In Vitro, Kinase Assay, Recombinant, SDS Page, Staining, High Performance Liquid Chromatography