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Promega ui rnasin
Ui Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ui rnasin/product/Promega
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ui rnasin - by Bioz Stars, 2020-04
86/100 stars

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total rna
200 ui m-mlv-rt

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Related Articles

Nucleic Acid Electrophoresis:

Article Title: Up-regulation of avian uncoupling protein in cold-acclimated and hyperthyroid ducklings prevents reactive oxygen species production by skeletal muscle mitochondria
Article Snippet: RNA concentrations were estimated by measuring the absorbance at 260 nm, and RNA integrity was checked by gel electrophoresis. .. Reverse transcription (RT) of 1 μg total RNA was performed as described previously [ ] using 200 UI M-MLV-RT (Promega, France), 2 μg/mL Poly T primers (Invitrogen, France), 1 mM deoxyribonucleotides and 25 UI RNAsin (Promega, France).

Amplification:

Article Title: Up-regulation of avian uncoupling protein in cold-acclimated and hyperthyroid ducklings prevents reactive oxygen species production by skeletal muscle mitochondria
Article Snippet: Reverse transcription (RT) of 1 μg total RNA was performed as described previously [ ] using 200 UI M-MLV-RT (Promega, France), 2 μg/mL Poly T primers (Invitrogen, France), 1 mM deoxyribonucleotides and 25 UI RNAsin (Promega, France). .. The amplified products were easily separated according to their size on 1.5% agarose gels (Sigma) stained with ethidium bromide (0.5 mg/mL, Sigma).

Article Title: Sphingomyelins and ceramides with VLCPUFAs are excluded from low-density raft-like domains in differentiating spermatogenic cells [S]
Article Snippet: Aliquots containing 2 μg total RNA were used to synthesize cDNA in reactions containing 1 μg Random Primers hexamers (Biodynamics), 1× M-MLV RT reaction buffer, 0.5 mM each of dNTP, 25 UI RNase inhibitor (Promega), and 200 UI M-MLV RT (Promega). .. The cDNA resulting from RT was amplified by real-time quantitative PCR (qPCR).

Article Title: Elovl4 and Fa2h expression during rat spermatogenesis: a link to the very-long-chain PUFAs typical of germ cell sphingolipids [S]
Article Snippet: The reverse transcription reaction vials contained 1 μg Random Primers hexamers (Biodynamics), 1× M-MLV RT reaction buffer, 0.5 mM each of dNTP, 25 UI RNase inhibitor (Promega), and 200 UI M-MLV RT (Promega). .. The gene-specific primer pairs designed for the amplification of rat Elovl s ( Elovl1 to Elovl7) are listed in .

Article Title: Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation Cycles
Article Snippet: Aliquots containing 2 μg total RNA were used to synthesize cDNA in reactions containing 1 μg Random Primer hexamers (Biodynamics), 1X M-MLV Reverse Transcriptase Reaction Buffer, 0.5 mM of each dNTP, 25 UI RNase inhibitor (Promega) and 200 UI M-MLV Reverse Transcriptase (Promega). .. The cDNA resulting from RT was amplified by real-time quantitative PCR (qPCR).

Isolation:

Article Title: Up-regulation of avian uncoupling protein in cold-acclimated and hyperthyroid ducklings prevents reactive oxygen species production by skeletal muscle mitochondria
Article Snippet: Total RNA was isolated from 0.1 g portions of frozen tissue, as described by Rey et al. [ ], using a Trizol solution (Euromedex, France). .. Reverse transcription (RT) of 1 μg total RNA was performed as described previously [ ] using 200 UI M-MLV-RT (Promega, France), 2 μg/mL Poly T primers (Invitrogen, France), 1 mM deoxyribonucleotides and 25 UI RNAsin (Promega, France).

Article Title: Sphingomyelins and ceramides with VLCPUFAs are excluded from low-density raft-like domains in differentiating spermatogenic cells [S]
Article Snippet: The total RNA from preparations containing the populations of spermatogenic cells, PtSs, RSs, and LSs, was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. .. Aliquots containing 2 μg total RNA were used to synthesize cDNA in reactions containing 1 μg Random Primers hexamers (Biodynamics), 1× M-MLV RT reaction buffer, 0.5 mM each of dNTP, 25 UI RNase inhibitor (Promega), and 200 UI M-MLV RT (Promega).

Article Title: Elovl4 and Fa2h expression during rat spermatogenesis: a link to the very-long-chain PUFAs typical of germ cell sphingolipids [S]
Article Snippet: The RNA from testes, STs, and cell preparations was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. .. The reverse transcription reaction vials contained 1 μg Random Primers hexamers (Biodynamics), 1× M-MLV RT reaction buffer, 0.5 mM each of dNTP, 25 UI RNase inhibitor (Promega), and 200 UI M-MLV RT (Promega).

Article Title: Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation Cycles
Article Snippet: Determination of Plin-2 mRNA levels Total RNA was isolated from dopaminergic neurons after the corresponding treatments using TRIZOL Reagent (Invitrogen) according to the manufacturer's protocol. .. Aliquots containing 2 μg total RNA were used to synthesize cDNA in reactions containing 1 μg Random Primer hexamers (Biodynamics), 1X M-MLV Reverse Transcriptase Reaction Buffer, 0.5 mM of each dNTP, 25 UI RNase inhibitor (Promega) and 200 UI M-MLV Reverse Transcriptase (Promega).

Spectrophotometry:

Article Title: Sphingomyelins and ceramides with VLCPUFAs are excluded from low-density raft-like domains in differentiating spermatogenic cells [S]
Article Snippet: The RNA was resuspended in RNase-free water and its concentration assessed from the A260:A280 absorbance ratio using a PicoDrop spectrophotometer. .. Aliquots containing 2 μg total RNA were used to synthesize cDNA in reactions containing 1 μg Random Primers hexamers (Biodynamics), 1× M-MLV RT reaction buffer, 0.5 mM each of dNTP, 25 UI RNase inhibitor (Promega), and 200 UI M-MLV RT (Promega).

Article Title: Elovl4 and Fa2h expression during rat spermatogenesis: a link to the very-long-chain PUFAs typical of germ cell sphingolipids [S]
Article Snippet: Total RNA samples were suspended in RNase-free water, their quality and concentrations were assessed using a PicoDrop spectrophotometer, and they were stored at −80°C until use. .. The reverse transcription reaction vials contained 1 μg Random Primers hexamers (Biodynamics), 1× M-MLV RT reaction buffer, 0.5 mM each of dNTP, 25 UI RNase inhibitor (Promega), and 200 UI M-MLV RT (Promega).

Article Title: Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation Cycles
Article Snippet: RNA was resuspended in nuclease-free water and its concentration was assessed from A260:A280 absorbance ratio in a PicoDrop Spectrophotometer. .. Aliquots containing 2 μg total RNA were used to synthesize cDNA in reactions containing 1 μg Random Primer hexamers (Biodynamics), 1X M-MLV Reverse Transcriptase Reaction Buffer, 0.5 mM of each dNTP, 25 UI RNase inhibitor (Promega) and 200 UI M-MLV Reverse Transcriptase (Promega).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Up-regulation of avian uncoupling protein in cold-acclimated and hyperthyroid ducklings prevents reactive oxygen species production by skeletal muscle mitochondria
Article Snippet: Relative abundance of avUCP transcripts Avian uncoupling protein (avUCP) expression was assessed by reverse transcription-polymerase chain reaction (RT-PCR). .. Reverse transcription (RT) of 1 μg total RNA was performed as described previously [ ] using 200 UI M-MLV-RT (Promega, France), 2 μg/mL Poly T primers (Invitrogen, France), 1 mM deoxyribonucleotides and 25 UI RNAsin (Promega, France).

Article Title: Elovl4 and Fa2h expression during rat spermatogenesis: a link to the very-long-chain PUFAs typical of germ cell sphingolipids [S]
Article Snippet: Paragraph title: RT-PCR of Elovls mRNAs. ... The reverse transcription reaction vials contained 1 μg Random Primers hexamers (Biodynamics), 1× M-MLV RT reaction buffer, 0.5 mM each of dNTP, 25 UI RNase inhibitor (Promega), and 200 UI M-MLV RT (Promega).

Real-time Polymerase Chain Reaction:

Article Title: Sphingomyelins and ceramides with VLCPUFAs are excluded from low-density raft-like domains in differentiating spermatogenic cells [S]
Article Snippet: Aliquots containing 2 μg total RNA were used to synthesize cDNA in reactions containing 1 μg Random Primers hexamers (Biodynamics), 1× M-MLV RT reaction buffer, 0.5 mM each of dNTP, 25 UI RNase inhibitor (Promega), and 200 UI M-MLV RT (Promega). .. The cDNA resulting from RT was amplified by real-time quantitative PCR (qPCR).

Article Title: Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation Cycles
Article Snippet: Aliquots containing 2 μg total RNA were used to synthesize cDNA in reactions containing 1 μg Random Primer hexamers (Biodynamics), 1X M-MLV Reverse Transcriptase Reaction Buffer, 0.5 mM of each dNTP, 25 UI RNase inhibitor (Promega) and 200 UI M-MLV Reverse Transcriptase (Promega). .. The cDNA resulting from RT was amplified by real-time quantitative PCR (qPCR).

Concentration Assay:

Article Title: Sphingomyelins and ceramides with VLCPUFAs are excluded from low-density raft-like domains in differentiating spermatogenic cells [S]
Article Snippet: The RNA was resuspended in RNase-free water and its concentration assessed from the A260:A280 absorbance ratio using a PicoDrop spectrophotometer. .. Aliquots containing 2 μg total RNA were used to synthesize cDNA in reactions containing 1 μg Random Primers hexamers (Biodynamics), 1× M-MLV RT reaction buffer, 0.5 mM each of dNTP, 25 UI RNase inhibitor (Promega), and 200 UI M-MLV RT (Promega).

Article Title: Dopaminergic Neurons Respond to Iron-Induced Oxidative Stress by Modulating Lipid Acylation and Deacylation Cycles
Article Snippet: RNA was resuspended in nuclease-free water and its concentration was assessed from A260:A280 absorbance ratio in a PicoDrop Spectrophotometer. .. Aliquots containing 2 μg total RNA were used to synthesize cDNA in reactions containing 1 μg Random Primer hexamers (Biodynamics), 1X M-MLV Reverse Transcriptase Reaction Buffer, 0.5 mM of each dNTP, 25 UI RNase inhibitor (Promega) and 200 UI M-MLV Reverse Transcriptase (Promega).

Quantitative RT-PCR:

Article Title: Sphingomyelins and ceramides with VLCPUFAs are excluded from low-density raft-like domains in differentiating spermatogenic cells [S]
Article Snippet: Aliquots containing 2 μg total RNA were used to synthesize cDNA in reactions containing 1 μg Random Primers hexamers (Biodynamics), 1× M-MLV RT reaction buffer, 0.5 mM each of dNTP, 25 UI RNase inhibitor (Promega), and 200 UI M-MLV RT (Promega). .. The RT-qPCR was performed in a final volume of 10 μl using KAPA SYBR FAST Master Mix (Kapa Biosystems) and 0.2 μM of each primer.

Expressing:

Article Title: Up-regulation of avian uncoupling protein in cold-acclimated and hyperthyroid ducklings prevents reactive oxygen species production by skeletal muscle mitochondria
Article Snippet: Relative abundance of avUCP transcripts Avian uncoupling protein (avUCP) expression was assessed by reverse transcription-polymerase chain reaction (RT-PCR). .. Reverse transcription (RT) of 1 μg total RNA was performed as described previously [ ] using 200 UI M-MLV-RT (Promega, France), 2 μg/mL Poly T primers (Invitrogen, France), 1 mM deoxyribonucleotides and 25 UI RNAsin (Promega, France).

Polymerase Chain Reaction:

Article Title: Up-regulation of avian uncoupling protein in cold-acclimated and hyperthyroid ducklings prevents reactive oxygen species production by skeletal muscle mitochondria
Article Snippet: Reverse transcription (RT) of 1 μg total RNA was performed as described previously [ ] using 200 UI M-MLV-RT (Promega, France), 2 μg/mL Poly T primers (Invitrogen, France), 1 mM deoxyribonucleotides and 25 UI RNAsin (Promega, France). .. PCR was then performed with one-tenth of the RT reaction in a total volume of 50 μL containing 0.2 mM dNTPs (Eurobio), 1 μM forward (5'-GTGGATGCCTACAGGACCAT-3') and 1 μM reverse (5'-ATGAACATCACCACGTTCCA-3') primers of avUCP, 2.5 U of Taq DNA polymerase (Eurobio), Taq reaction buffer (Eurobio) and 1.5 mM MgCl2 in a Hybaid thermocycler (Ashford).

Article Title: Elovl4 and Fa2h expression during rat spermatogenesis: a link to the very-long-chain PUFAs typical of germ cell sphingolipids [S]
Article Snippet: The reverse transcription reaction vials contained 1 μg Random Primers hexamers (Biodynamics), 1× M-MLV RT reaction buffer, 0.5 mM each of dNTP, 25 UI RNase inhibitor (Promega), and 200 UI M-MLV RT (Promega). .. Of the cDNA solution, 3 μl were used for the PCR reaction with Taq DNA polymerase, carried out in a volume of 20 μl.

Staining:

Article Title: Up-regulation of avian uncoupling protein in cold-acclimated and hyperthyroid ducklings prevents reactive oxygen species production by skeletal muscle mitochondria
Article Snippet: Reverse transcription (RT) of 1 μg total RNA was performed as described previously [ ] using 200 UI M-MLV-RT (Promega, France), 2 μg/mL Poly T primers (Invitrogen, France), 1 mM deoxyribonucleotides and 25 UI RNAsin (Promega, France). .. The amplified products were easily separated according to their size on 1.5% agarose gels (Sigma) stained with ethidium bromide (0.5 mg/mL, Sigma).

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    Promega ui rnase inhibitor
    Ui Rnase Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ui rnase inhibitor/product/Promega
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ui rnase inhibitor - by Bioz Stars, 2020-04
    92/100 stars
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