udp n glycosylase user  (New England Biolabs)


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    Structured Review

    New England Biolabs udp n glycosylase user
    Udp N Glycosylase User, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/udp n glycosylase user/product/New England Biolabs
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    udp n glycosylase user - by Bioz Stars, 2020-04
    89/100 stars

    Related Products / Commonly Used Together

    dutp
    pcr polymerase
    du-containing dna strand

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    Related Articles

    RNA Sequencing Assay:

    Article Title: RNA-seq of human reference RNA samples using a thermostable group II intron reverse transcriptase
    Article Snippet: The preservation of strand information in RNA-seq has become increasingly important in light of the growing appreciation of the extent and significance of antisense transcription ( ). .. Widely used strand-specific methods involve the incorporation of dUTP during second-strand synthesis, yielding a dU-containing DNA strand that is either degraded by UDP-N-glycosylase/USER ( ; NEBNext Ultra Directional RNA Library Prep Kit) or excluded by using a PCR polymerase that stalls at dU sites in DNA (Illumina TruSeq v3).

    Ligation:

    Article Title: RNA-seq of human reference RNA samples using a thermostable group II intron reverse transcriptase
    Article Snippet: This and similar methods using sequential RNA-seq adapter ligation are typically time consuming, have well-documented sequence and secondary structure biases, and can yield undesirable side products ( ; ; ). .. Widely used strand-specific methods involve the incorporation of dUTP during second-strand synthesis, yielding a dU-containing DNA strand that is either degraded by UDP-N-glycosylase/USER ( ; NEBNext Ultra Directional RNA Library Prep Kit) or excluded by using a PCR polymerase that stalls at dU sites in DNA (Illumina TruSeq v3).

    Preserving:

    Article Title: RNA-seq of human reference RNA samples using a thermostable group II intron reverse transcriptase
    Article Snippet: The preservation of strand information in RNA-seq has become increasingly important in light of the growing appreciation of the extent and significance of antisense transcription ( ). .. Widely used strand-specific methods involve the incorporation of dUTP during second-strand synthesis, yielding a dU-containing DNA strand that is either degraded by UDP-N-glycosylase/USER ( ; NEBNext Ultra Directional RNA Library Prep Kit) or excluded by using a PCR polymerase that stalls at dU sites in DNA (Illumina TruSeq v3).

    Nick Translation:

    Article Title: RNA-seq of human reference RNA samples using a thermostable group II intron reverse transcriptase
    Article Snippet: Second-strand synthesis is performed via nick translation by using RNase H and DNA polymerase I, and the resulting double-stranded cDNAs are 3′ A-tailed and ligated to double-stranded DNA adapters at both ends, thus erasing strand information. .. Widely used strand-specific methods involve the incorporation of dUTP during second-strand synthesis, yielding a dU-containing DNA strand that is either degraded by UDP-N-glycosylase/USER ( ; NEBNext Ultra Directional RNA Library Prep Kit) or excluded by using a PCR polymerase that stalls at dU sites in DNA (Illumina TruSeq v3).

    Polymerase Chain Reaction:

    Article Title: RNA-seq of human reference RNA samples using a thermostable group II intron reverse transcriptase
    Article Snippet: .. Widely used strand-specific methods involve the incorporation of dUTP during second-strand synthesis, yielding a dU-containing DNA strand that is either degraded by UDP-N-glycosylase/USER ( ; NEBNext Ultra Directional RNA Library Prep Kit) or excluded by using a PCR polymerase that stalls at dU sites in DNA (Illumina TruSeq v3). .. In both the NEBNext and Illumina protocols, antisense artifacts from re-copying of the cDNA by the retroviral RT are mitigated by adding actinomycin D, which inhibits DNA-dependent DNA synthesis ( ; ).

    Random Hexamer Labeling:

    Article Title: RNA-seq of human reference RNA samples using a thermostable group II intron reverse transcriptase
    Article Snippet: The commonly used non-strand-specific Illumina TruSeq v2 method starts with fragmented RNA (either ribo-depleted or poly(A)-selected) and uses random-hexamer priming for cDNA synthesis by SuperScript II RT, a derivative of the retroviral M-MLV RT. .. Widely used strand-specific methods involve the incorporation of dUTP during second-strand synthesis, yielding a dU-containing DNA strand that is either degraded by UDP-N-glycosylase/USER ( ; NEBNext Ultra Directional RNA Library Prep Kit) or excluded by using a PCR polymerase that stalls at dU sites in DNA (Illumina TruSeq v3).

    Sequencing:

    Article Title: RNA-seq of human reference RNA samples using a thermostable group II intron reverse transcriptase
    Article Snippet: This and similar methods using sequential RNA-seq adapter ligation are typically time consuming, have well-documented sequence and secondary structure biases, and can yield undesirable side products ( ; ; ). .. Widely used strand-specific methods involve the incorporation of dUTP during second-strand synthesis, yielding a dU-containing DNA strand that is either degraded by UDP-N-glycosylase/USER ( ; NEBNext Ultra Directional RNA Library Prep Kit) or excluded by using a PCR polymerase that stalls at dU sites in DNA (Illumina TruSeq v3).

    DNA Synthesis:

    Article Title: RNA-seq of human reference RNA samples using a thermostable group II intron reverse transcriptase
    Article Snippet: Widely used strand-specific methods involve the incorporation of dUTP during second-strand synthesis, yielding a dU-containing DNA strand that is either degraded by UDP-N-glycosylase/USER ( ; NEBNext Ultra Directional RNA Library Prep Kit) or excluded by using a PCR polymerase that stalls at dU sites in DNA (Illumina TruSeq v3). .. In both the NEBNext and Illumina protocols, antisense artifacts from re-copying of the cDNA by the retroviral RT are mitigated by adding actinomycin D, which inhibits DNA-dependent DNA synthesis ( ; ).

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  • 89
    New England Biolabs udp n glycosylase user
    Udp N Glycosylase User, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/udp n glycosylase user/product/New England Biolabs
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    udp n glycosylase user - by Bioz Stars, 2020-04
    89/100 stars
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