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GE Healthcare typhoon 9400 laser scanner
Typhoon 9400 Laser Scanner, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/typhoon 9400 laser scanner/product/GE Healthcare
Average 93 stars, based on 18 article reviews
Price from $9.99 to $1999.99
typhoon 9400 laser scanner - by Bioz Stars, 2020-04
93/100 stars

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Amplification:

Article Title: Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs
Article Snippet: DNA labeling by end filling using Cy3-dNTP DNA sequences containing two BtsCI recognition sites were amplified from pUC19 using LongAmp Taq 2X master mix and digested by the indicated endonuclease(s). .. Labeled DNA was visualized using Typhoon 9400 laser scanner (GE Life Sciences; excitation—532 nm; emission—580 nm).

Spectroscopy:

Article Title: Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression of α-Tubulin Isoforms
Article Snippet: .. The SDS-PAGE gels were imaged by sequential fluorescence emission spectroscopy using a Typhoon 9400 laser scanner (GE Healthcare, Piscataway, NJ) for each of the spectrally resolvable Cy3/5 dyes (Cy3, 532 nm/580 [bp ± 15] nm; Cy5, 633 nm/670 [bp ± 15]) and digital images recorded using ImageQuant (GE Healthcare) software. .. Individual protein spots observed within each of the 686Ln (Cy3) or 686Tu (Cy5) images were then quantified and compared after volumetric rendering using the differential in-gel analysis (DIA) algorithm within DeCyder (v5.5; GE Healthcare) software.

Electrophoresis:

Article Title: Identification of Clinically Relevant Protein Targets in Prostate Cancer with 2D-DIGE Coupled Mass Spectrometry and Systems Biology Network Platform
Article Snippet: Following electrophoresis, analytical gel cassettes were washed with ddH2 O and wiped with dust free tissue paper. .. The Cy2 (internal standard), Cy3 and Cy5 labelled proteins in each gel were visualized by using a Typhoon 9400™ laser scanner (GE Healthcare) at 100 microns density by using different excitation and emission wavelengths directly from gels between glass plates.

Article Title: Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression of α-Tubulin Isoforms
Article Snippet: Paragraph title: 2.3. Ettan DIGE Electrophoresis and Peptide Sequencing ... The SDS-PAGE gels were imaged by sequential fluorescence emission spectroscopy using a Typhoon 9400 laser scanner (GE Healthcare, Piscataway, NJ) for each of the spectrally resolvable Cy3/5 dyes (Cy3, 532 nm/580 [bp ± 15] nm; Cy5, 633 nm/670 [bp ± 15]) and digital images recorded using ImageQuant (GE Healthcare) software.

Article Title: The role of protein modifications in senescence of freeze-dried Acetobacter senegalensis during storage
Article Snippet: Each strip was then treated for reduction/alkylation and the second dimension of electrophoresis was run as mentioned above. .. Then, the membranes were washed three times in TTBS buffer and incubated with the second antibody (1/2500) at room temperature for 1 h. Finally, after three washings with TBS buffer, the membranes were scanned with a Typhoon 9400 Laser Scanner (GE Healthcare) at three different wavelengths corresponding to the different CyDyes and Anti-DNP antibody.

Article Title: Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells
Article Snippet: Afterward, 150 µg of protein was applied to a 24-cm gel strip and separated by second-dimension electrophoresis using the Ettan DALTsix Electrophoresis system (GE Healthcare). .. The fluorescence intensities of Cy-labelled proteins were quantified by scanning the gels at each excitation wavelength (488 nm/520 nm for Cy2, 532 nm/580 nm for Cy3, and 633 nm/670 nm for Cy5) using a Typhoon 9400 laser scanner (GE Healthcare).

Incubation:

Article Title: The role of protein modifications in senescence of freeze-dried Acetobacter senegalensis during storage
Article Snippet: .. Then, the membranes were washed three times in TTBS buffer and incubated with the second antibody (1/2500) at room temperature for 1 h. Finally, after three washings with TBS buffer, the membranes were scanned with a Typhoon 9400 Laser Scanner (GE Healthcare) at three different wavelengths corresponding to the different CyDyes and Anti-DNP antibody. .. 2-D gel analysis software (DeCyder version 7.0; GE Healthcare) was used for spot detection, gel matching and spot quantification relative to the corresponding spot in the internal standard.

Article Title: Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation
Article Snippet: The membrane was washed with TTBS three times (10 minutes each), incubated in either ECL Plex goat-anti-mouse IgG Cy3 or goat-anti-rabbit Cy5-conjugated secondary antibody (dilution 1/2500, GE Healthcare) for 1 hour at room temperature and washed again three times in TTBS (15 minutes each). .. After drying at +37°C, membranes were scanned at the corresponding wavelengths with a Typhoon 9400 Laser Scanner (GE Healthcare).

Article Title: Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells
Article Snippet: For sample equilibration, the IPG gel strips were incubated with 10 mL of a solution containing 50 mM Tris-HCl (pH 6.8), 6 M urea, 2% (w/v) SDS, 0.002% bromophenol blue, 30% glycerol, and 100 mg DTT for 30 min and then with 10 mL of a solution containing 50 mM Tris-HCl (pH 6.8), 6 M urea, 2% (w/v) SDS, 0.002% bromophenol blue, 30% glycerol and 250 mg iodoacetamide for 15 min. .. The fluorescence intensities of Cy-labelled proteins were quantified by scanning the gels at each excitation wavelength (488 nm/520 nm for Cy2, 532 nm/580 nm for Cy3, and 633 nm/670 nm for Cy5) using a Typhoon 9400 laser scanner (GE Healthcare).

Stripping Membranes:

Article Title: The role of protein modifications in senescence of freeze-dried Acetobacter senegalensis during storage
Article Snippet: Each strip was then treated for reduction/alkylation and the second dimension of electrophoresis was run as mentioned above. .. Then, the membranes were washed three times in TTBS buffer and incubated with the second antibody (1/2500) at room temperature for 1 h. Finally, after three washings with TBS buffer, the membranes were scanned with a Typhoon 9400 Laser Scanner (GE Healthcare) at three different wavelengths corresponding to the different CyDyes and Anti-DNP antibody.

Article Title: Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells
Article Snippet: Afterward, 150 µg of protein was applied to a 24-cm gel strip and separated by second-dimension electrophoresis using the Ettan DALTsix Electrophoresis system (GE Healthcare). .. The fluorescence intensities of Cy-labelled proteins were quantified by scanning the gels at each excitation wavelength (488 nm/520 nm for Cy2, 532 nm/580 nm for Cy3, and 633 nm/670 nm for Cy5) using a Typhoon 9400 laser scanner (GE Healthcare).

Mass Spectrometry:

Article Title: Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression of α-Tubulin Isoforms
Article Snippet: The SDS-PAGE gels were imaged by sequential fluorescence emission spectroscopy using a Typhoon 9400 laser scanner (GE Healthcare, Piscataway, NJ) for each of the spectrally resolvable Cy3/5 dyes (Cy3, 532 nm/580 [bp ± 15] nm; Cy5, 633 nm/670 [bp ± 15]) and digital images recorded using ImageQuant (GE Healthcare) software. .. In a conventional 2D electrophoresis under the same conditions, the interested spots were picked after Coomassie blue staining for further mass spectrometry.

BIA-KA:

Article Title: Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression of α-Tubulin Isoforms
Article Snippet: Ettan DIGE Electrophoresis and Peptide Sequencing The proteins were extracted from cell lines and quantitated with BCA protein assay kit (Pierce). .. The SDS-PAGE gels were imaged by sequential fluorescence emission spectroscopy using a Typhoon 9400 laser scanner (GE Healthcare, Piscataway, NJ) for each of the spectrally resolvable Cy3/5 dyes (Cy3, 532 nm/580 [bp ± 15] nm; Cy5, 633 nm/670 [bp ± 15]) and digital images recorded using ImageQuant (GE Healthcare) software.

Western Blot:

Article Title: The role of protein modifications in senescence of freeze-dried Acetobacter senegalensis during storage
Article Snippet: Paragraph title: Determination of carbonylated proteins by western blotting ... Then, the membranes were washed three times in TTBS buffer and incubated with the second antibody (1/2500) at room temperature for 1 h. Finally, after three washings with TBS buffer, the membranes were scanned with a Typhoon 9400 Laser Scanner (GE Healthcare) at three different wavelengths corresponding to the different CyDyes and Anti-DNP antibody.

Article Title: Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation
Article Snippet: Paragraph title: Western blots ... After drying at +37°C, membranes were scanned at the corresponding wavelengths with a Typhoon 9400 Laser Scanner (GE Healthcare).

Kinase Assay:

Article Title: Signal Transduction Protein Array Analysis Links LRRK2 to Ste20 Kinases and PKC Zeta That Modulate Neuronal Plasticity
Article Snippet: Paragraph title: Kinase assay in solution ... Phosphoscreens were imaged on a Typhoon 9400 laser scanner (GE Healthcare, Freiburg, Germany) and quantified using Quantity One software (Biorad, Munich, Germany).

Derivative Assay:

Article Title: Identification of chaperones in a MPP+-induced and ATRA/TPA-differentiated SH-SY5Y cell PD model
Article Snippet: Half of the samples from each group were labeled with Cy3, and another half were labeled with Cy5 to avoid any possible bias derived from dye labeling. .. DIGE gels were scanned with a Typhoon 9400 laser scanner (Amersham Biosciences-GE Healthcare, Uppsala, Sweden) at a resolution of 100 µm in three different wavelengths to obtain individual images of the Cy2, Cy3 and Cy5 components of each gel.

DNA Labeling:

Article Title: Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs
Article Snippet: Paragraph title: DNA labeling by end filling using Cy3-dNTP ... Labeled DNA was visualized using Typhoon 9400 laser scanner (GE Life Sciences; excitation—532 nm; emission—580 nm).

Sequencing:

Article Title: Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression of α-Tubulin Isoforms
Article Snippet: Paragraph title: 2.3. Ettan DIGE Electrophoresis and Peptide Sequencing ... The SDS-PAGE gels were imaged by sequential fluorescence emission spectroscopy using a Typhoon 9400 laser scanner (GE Healthcare, Piscataway, NJ) for each of the spectrally resolvable Cy3/5 dyes (Cy3, 532 nm/580 [bp ± 15] nm; Cy5, 633 nm/670 [bp ± 15]) and digital images recorded using ImageQuant (GE Healthcare) software.

Recombinant:

Article Title: Signal Transduction Protein Array Analysis Links LRRK2 to Ste20 Kinases and PKC Zeta That Modulate Neuronal Plasticity
Article Snippet: Full-length LRRK2, recombinant GST (GenWay, San Diego, USA), and GST-tagged moesin (University of Dundee, Dundee, UK) were assayed as well. .. Phosphoscreens were imaged on a Typhoon 9400 laser scanner (GE Healthcare, Freiburg, Germany) and quantified using Quantity One software (Biorad, Munich, Germany).

Molecular Weight:

Article Title: Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation
Article Snippet: A molecular weight ladder was separated along with the proteins of interest (MagicMark, Invitrogen). .. After drying at +37°C, membranes were scanned at the corresponding wavelengths with a Typhoon 9400 Laser Scanner (GE Healthcare).

Nucleic Acid Electrophoresis:

Article Title: Identification of Clinically Relevant Protein Targets in Prostate Cancer with 2D-DIGE Coupled Mass Spectrometry and Systems Biology Network Platform
Article Snippet: Paragraph title: DIGE-Two-dimensional gel electrophoresis (DIGE-2-DE) ... The Cy2 (internal standard), Cy3 and Cy5 labelled proteins in each gel were visualized by using a Typhoon 9400™ laser scanner (GE Healthcare) at 100 microns density by using different excitation and emission wavelengths directly from gels between glass plates.

Article Title: Differential Response in Downstream Processing of CHO Cells Grown Under Mild Hypothermic Conditions
Article Snippet: Paragraph title: 2D-Polyacrylamide Gel Electrophoresis (2D-PAGE) ... The gels were then stained with Sypro Ruby (Invitrogen), according to the manufacturer's protocol and scanned using a Typhoon 9400 laser scanner (GE Healthcare), with the following settings; 600 V PMT, 532 nm (green laser) for excitation and 610BP30 emission filter and a pixel size of 100 µm.

Fluorescence:

Article Title: Identification of Clinically Relevant Protein Targets in Prostate Cancer with 2D-DIGE Coupled Mass Spectrometry and Systems Biology Network Platform
Article Snippet: Polyacrylamide gels (12.5%) were cast in low fluorescence glass plates. .. The Cy2 (internal standard), Cy3 and Cy5 labelled proteins in each gel were visualized by using a Typhoon 9400™ laser scanner (GE Healthcare) at 100 microns density by using different excitation and emission wavelengths directly from gels between glass plates.

Article Title: Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression of α-Tubulin Isoforms
Article Snippet: .. The SDS-PAGE gels were imaged by sequential fluorescence emission spectroscopy using a Typhoon 9400 laser scanner (GE Healthcare, Piscataway, NJ) for each of the spectrally resolvable Cy3/5 dyes (Cy3, 532 nm/580 [bp ± 15] nm; Cy5, 633 nm/670 [bp ± 15]) and digital images recorded using ImageQuant (GE Healthcare) software. .. Individual protein spots observed within each of the 686Ln (Cy3) or 686Tu (Cy5) images were then quantified and compared after volumetric rendering using the differential in-gel analysis (DIA) algorithm within DeCyder (v5.5; GE Healthcare) software.

Article Title: Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells
Article Snippet: .. The fluorescence intensities of Cy-labelled proteins were quantified by scanning the gels at each excitation wavelength (488 nm/520 nm for Cy2, 532 nm/580 nm for Cy3, and 633 nm/670 nm for Cy5) using a Typhoon 9400 laser scanner (GE Healthcare). ..

Labeling:

Article Title: Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs
Article Snippet: .. Labeled DNA was visualized using Typhoon 9400 laser scanner (GE Life Sciences; excitation—532 nm; emission—580 nm). .. Total DNA was visualized by UV illumination after staining with ethidium bromide (2 µg/ml).

Article Title: Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression of α-Tubulin Isoforms
Article Snippet: The two labeled samples were combined and further mixed with 2x loading buffer for isoelectric focusing (IEF, 7 m urea, 2 M thiourea, 2% CHAPS, 50 mM DTT and 1% Phamalyte (pH3–10NL). .. The SDS-PAGE gels were imaged by sequential fluorescence emission spectroscopy using a Typhoon 9400 laser scanner (GE Healthcare, Piscataway, NJ) for each of the spectrally resolvable Cy3/5 dyes (Cy3, 532 nm/580 [bp ± 15] nm; Cy5, 633 nm/670 [bp ± 15]) and digital images recorded using ImageQuant (GE Healthcare) software.

Article Title: Identification of chaperones in a MPP+-induced and ATRA/TPA-differentiated SH-SY5Y cell PD model
Article Snippet: The internal standard, which is a pool of equal amounts of all experimental samples, were labeled with Cy2. .. DIGE gels were scanned with a Typhoon 9400 laser scanner (Amersham Biosciences-GE Healthcare, Uppsala, Sweden) at a resolution of 100 µm in three different wavelengths to obtain individual images of the Cy2, Cy3 and Cy5 components of each gel.

Polymerase Chain Reaction:

Article Title: Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs
Article Snippet: PCR product (0.1 µg) was labeled with Cy3-dNTP (PerkinElmer) by E. coli Pol I Klenow fragment at room temperature for 15 min and then analyzed by 6% PAGE in 1 × TBE. .. Labeled DNA was visualized using Typhoon 9400 laser scanner (GE Life Sciences; excitation—532 nm; emission—580 nm).

Polyacrylamide Gel Electrophoresis:

Article Title: Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs
Article Snippet: PCR product (0.1 µg) was labeled with Cy3-dNTP (PerkinElmer) by E. coli Pol I Klenow fragment at room temperature for 15 min and then analyzed by 6% PAGE in 1 × TBE. .. Labeled DNA was visualized using Typhoon 9400 laser scanner (GE Life Sciences; excitation—532 nm; emission—580 nm).

Staining:

Article Title: Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs
Article Snippet: Labeled DNA was visualized using Typhoon 9400 laser scanner (GE Life Sciences; excitation—532 nm; emission—580 nm). .. Total DNA was visualized by UV illumination after staining with ethidium bromide (2 µg/ml).

Article Title: Identification of Clinically Relevant Protein Targets in Prostate Cancer with 2D-DIGE Coupled Mass Spectrometry and Systems Biology Network Platform
Article Snippet: The Cy2 (internal standard), Cy3 and Cy5 labelled proteins in each gel were visualized by using a Typhoon 9400™ laser scanner (GE Healthcare) at 100 microns density by using different excitation and emission wavelengths directly from gels between glass plates. .. Preparative gels were stained with Roti®-Blue, a colloidal coomassie brilliant blue G250 stain.

Article Title: Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression of α-Tubulin Isoforms
Article Snippet: The SDS-PAGE gels were imaged by sequential fluorescence emission spectroscopy using a Typhoon 9400 laser scanner (GE Healthcare, Piscataway, NJ) for each of the spectrally resolvable Cy3/5 dyes (Cy3, 532 nm/580 [bp ± 15] nm; Cy5, 633 nm/670 [bp ± 15]) and digital images recorded using ImageQuant (GE Healthcare) software. .. In a conventional 2D electrophoresis under the same conditions, the interested spots were picked after Coomassie blue staining for further mass spectrometry.

Article Title: Differential Response in Downstream Processing of CHO Cells Grown Under Mild Hypothermic Conditions
Article Snippet: .. The gels were then stained with Sypro Ruby (Invitrogen), according to the manufacturer's protocol and scanned using a Typhoon 9400 laser scanner (GE Healthcare), with the following settings; 600 V PMT, 532 nm (green laser) for excitation and 610BP30 emission filter and a pixel size of 100 µm. .. Progenesis Samespots software was used for spot comparison (version 4.0, Nonlinear Dynamics, Newcastle upon Tyne, UK).

Article Title: Probiotic Gut Microbiota Isolate Interacts with Dendritic Cells via Glycosylated Heterotrimeric Pili
Article Snippet: .. Sypro® and PAS stained gels were scanned with a Typhoon 9400 laser scanner. .. All assays were performed at least in triplicate for all purified pili fractions to verify the data.

SDS Page:

Article Title: Identification of Clinically Relevant Protein Targets in Prostate Cancer with 2D-DIGE Coupled Mass Spectrometry and Systems Biology Network Platform
Article Snippet: The equilibrated strips were applied to the top of 12.5% SDS-PAGE gels and sealed with 1% agarose prepared in SDS-Tris-glycine buffer with traces of bromophenol blue as a tracking dye to monitor electrophoresis. .. The Cy2 (internal standard), Cy3 and Cy5 labelled proteins in each gel were visualized by using a Typhoon 9400™ laser scanner (GE Healthcare) at 100 microns density by using different excitation and emission wavelengths directly from gels between glass plates.

Article Title: Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression of α-Tubulin Isoforms
Article Snippet: .. The SDS-PAGE gels were imaged by sequential fluorescence emission spectroscopy using a Typhoon 9400 laser scanner (GE Healthcare, Piscataway, NJ) for each of the spectrally resolvable Cy3/5 dyes (Cy3, 532 nm/580 [bp ± 15] nm; Cy5, 633 nm/670 [bp ± 15]) and digital images recorded using ImageQuant (GE Healthcare) software. .. Individual protein spots observed within each of the 686Ln (Cy3) or 686Tu (Cy5) images were then quantified and compared after volumetric rendering using the differential in-gel analysis (DIA) algorithm within DeCyder (v5.5; GE Healthcare) software.

Article Title: Differential Response in Downstream Processing of CHO Cells Grown Under Mild Hypothermic Conditions
Article Snippet: 2D-Polyacrylamide Gel Electrophoresis (2D-PAGE) Samples from the relevant timepoints of each culture were prepared and Isoelectric focussing and SDS-PAGE performed as previously described. .. The gels were then stained with Sypro Ruby (Invitrogen), according to the manufacturer's protocol and scanned using a Typhoon 9400 laser scanner (GE Healthcare), with the following settings; 600 V PMT, 532 nm (green laser) for excitation and 610BP30 emission filter and a pixel size of 100 µm.

Software:

Article Title: Proteomic Analysis of the Reproductive Organs of the Hermaphroditic Gastropod Lymnaea stagnalis Exposed to Different Endocrine Disrupting Chemicals
Article Snippet: Image Acquisition and Analysis Gels were scanned using a Typhoon 9400 Laser scanner (GE Healthcare, Piscataway, NJ, United States) with excitation and emission wavelengths specific to each CyDye (Cy2: 488/520; Cy3: 523/580 and Cy5: 633/670 nm). .. 2D-Gel images were analysed using Decyder 2D Differential analysis software (v.7.0, GE Healthcare, Piscataway, NJ, United States).

Article Title: Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression of α-Tubulin Isoforms
Article Snippet: .. The SDS-PAGE gels were imaged by sequential fluorescence emission spectroscopy using a Typhoon 9400 laser scanner (GE Healthcare, Piscataway, NJ) for each of the spectrally resolvable Cy3/5 dyes (Cy3, 532 nm/580 [bp ± 15] nm; Cy5, 633 nm/670 [bp ± 15]) and digital images recorded using ImageQuant (GE Healthcare) software. .. Individual protein spots observed within each of the 686Ln (Cy3) or 686Tu (Cy5) images were then quantified and compared after volumetric rendering using the differential in-gel analysis (DIA) algorithm within DeCyder (v5.5; GE Healthcare) software.

Article Title: Signal Transduction Protein Array Analysis Links LRRK2 to Ste20 Kinases and PKC Zeta That Modulate Neuronal Plasticity
Article Snippet: .. Phosphoscreens were imaged on a Typhoon 9400 laser scanner (GE Healthcare, Freiburg, Germany) and quantified using Quantity One software (Biorad, Munich, Germany). .. Immunoprecipitation Animal work was approved by the Regierungspraesidium Tuebingen (ID 10-004).

Article Title: Signal Transduction Protein Array Analysis Links LRRK2 to Ste20 Kinases and PKC Zeta That Modulate Neuronal Plasticity
Article Snippet: .. The arrays were imaged directly on a Typhoon 9400 laser scanner and analysed by Genepix Pro followed by ProtoArray Prospector software (see ). .. As can be seen from , a significantly higher green fluorescence signal of the quadruplicate PKC zeta spots was detected when compared to the corresponding spots on the control arrays.

Article Title: Identification of chaperones in a MPP+-induced and ATRA/TPA-differentiated SH-SY5Y cell PD model
Article Snippet: DIGE gels were scanned with a Typhoon 9400 laser scanner (Amersham Biosciences-GE Healthcare, Uppsala, Sweden) at a resolution of 100 µm in three different wavelengths to obtain individual images of the Cy2, Cy3 and Cy5 components of each gel. .. Gel analysis was carried out using the DeCyder Differential In-gel Analysis software (Amersham Biosciences-GE Healthcare, Uppsala, Sweden) to co-detect and quantify protein spots in images according to the protocols provided by the manufacturer.

Article Title: Differential Response in Downstream Processing of CHO Cells Grown Under Mild Hypothermic Conditions
Article Snippet: The gels were then stained with Sypro Ruby (Invitrogen), according to the manufacturer's protocol and scanned using a Typhoon 9400 laser scanner (GE Healthcare), with the following settings; 600 V PMT, 532 nm (green laser) for excitation and 610BP30 emission filter and a pixel size of 100 µm. .. Progenesis Samespots software was used for spot comparison (version 4.0, Nonlinear Dynamics, Newcastle upon Tyne, UK).

Article Title: The role of protein modifications in senescence of freeze-dried Acetobacter senegalensis during storage
Article Snippet: Then, the membranes were washed three times in TTBS buffer and incubated with the second antibody (1/2500) at room temperature for 1 h. Finally, after three washings with TBS buffer, the membranes were scanned with a Typhoon 9400 Laser Scanner (GE Healthcare) at three different wavelengths corresponding to the different CyDyes and Anti-DNP antibody. .. 2-D gel analysis software (DeCyder version 7.0; GE Healthcare) was used for spot detection, gel matching and spot quantification relative to the corresponding spot in the internal standard.

Article Title: Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation
Article Snippet: After drying at +37°C, membranes were scanned at the corresponding wavelengths with a Typhoon 9400 Laser Scanner (GE Healthcare). .. Membrane images were analyzed with the ImageMaster 1D software (GE Healthcare).

Article Title: Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells
Article Snippet: The fluorescence intensities of Cy-labelled proteins were quantified by scanning the gels at each excitation wavelength (488 nm/520 nm for Cy2, 532 nm/580 nm for Cy3, and 633 nm/670 nm for Cy5) using a Typhoon 9400 laser scanner (GE Healthcare). .. Quantitative analysis of the protein patterns was performed using the image analysis software DeCyder (GE Healthcare).

Concentration Assay:

Article Title: Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs
Article Snippet: Digestion was terminated by adding EDTA to a final concentration of 20 mM. .. Labeled DNA was visualized using Typhoon 9400 laser scanner (GE Life Sciences; excitation—532 nm; emission—580 nm).

Two-Dimensional Gel Electrophoresis:

Article Title: Proteomic Analysis of the Reproductive Organs of the Hermaphroditic Gastropod Lymnaea stagnalis Exposed to Different Endocrine Disrupting Chemicals
Article Snippet: Image Acquisition and Analysis Gels were scanned using a Typhoon 9400 Laser scanner (GE Healthcare, Piscataway, NJ, United States) with excitation and emission wavelengths specific to each CyDye (Cy2: 488/520; Cy3: 523/580 and Cy5: 633/670 nm). .. 2D-Gel images were analysed using Decyder 2D Differential analysis software (v.7.0, GE Healthcare, Piscataway, NJ, United States).

Article Title: Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression of α-Tubulin Isoforms
Article Snippet: The SDS-PAGE gels were imaged by sequential fluorescence emission spectroscopy using a Typhoon 9400 laser scanner (GE Healthcare, Piscataway, NJ) for each of the spectrally resolvable Cy3/5 dyes (Cy3, 532 nm/580 [bp ± 15] nm; Cy5, 633 nm/670 [bp ± 15]) and digital images recorded using ImageQuant (GE Healthcare) software. .. In a conventional 2D electrophoresis under the same conditions, the interested spots were picked after Coomassie blue staining for further mass spectrometry.

Lysis:

Article Title: Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells
Article Snippet: A total of 150 µg Cy-labeled protein in a final volume of 450 µL lysis buffer containing 8 M urea (GE Healthcare), 4% CHAPS (GE Healthcare), 1.2% DeStreak solution (GE Healthcare), and 0.5% IPG buffer 3–11 NL (GE Healthcare) was loaded onto the IPG gel strips, and was rehydrated in the dark at room temperature overnight. .. The fluorescence intensities of Cy-labelled proteins were quantified by scanning the gels at each excitation wavelength (488 nm/520 nm for Cy2, 532 nm/580 nm for Cy3, and 633 nm/670 nm for Cy5) using a Typhoon 9400 laser scanner (GE Healthcare).

Electrofocusing:

Article Title: Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression of α-Tubulin Isoforms
Article Snippet: The first-dimension IEF on a rod gel (12 cm × F1.0 mm, pH3–10) was performed under a programmed gradient 200 V to 1,500 V over 18 hrs (total 25 kVh). .. The SDS-PAGE gels were imaged by sequential fluorescence emission spectroscopy using a Typhoon 9400 laser scanner (GE Healthcare, Piscataway, NJ) for each of the spectrally resolvable Cy3/5 dyes (Cy3, 532 nm/580 [bp ± 15] nm; Cy5, 633 nm/670 [bp ± 15]) and digital images recorded using ImageQuant (GE Healthcare) software.

Article Title: Identification of chaperones in a MPP+-induced and ATRA/TPA-differentiated SH-SY5Y cell PD model
Article Snippet: Isoelectric focusing (IEF) was carried out on an IPGphor system (Amersham Biosciences-GE Healthcare, Uppsala, Sweden) using 24-cm IPG gel strips (pH 4-7) at 20°C under the following conditions: six hours at 0 V for passive rehydration, six hours at 30 V for active rehydration, three hours at 500 V, one hour each at gradients of 1,000 V and 8,000 V, and finally, seven hours at 8,000 V. Focused IPG strips were reduced for 15 minutes in equilibration buffer I (50 mM of Tris pH 8.8, 6 M of urea, 30% glycerol, 2% SDS, 0.2% bromophenol blue, and 1% DTT) and alkylated for 15 minutes in equilibration buffer II (buffer II was identical to buffer I, but with 2.5% iodoacetamide instead of DTT). .. DIGE gels were scanned with a Typhoon 9400 laser scanner (Amersham Biosciences-GE Healthcare, Uppsala, Sweden) at a resolution of 100 µm in three different wavelengths to obtain individual images of the Cy2, Cy3 and Cy5 components of each gel.

Article Title: Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells
Article Snippet: IEF was performed using an Ettan IPGPhor system (GE Healthcare) under the following condition: held at 100 V for 2 h, held at 500 V for 1 h, ramped to 1000 V in 1 h, ramped to 8000 V in 3 h, and held at 8000 V for 8 h for pH 3–11 NL gel strips. .. The fluorescence intensities of Cy-labelled proteins were quantified by scanning the gels at each excitation wavelength (488 nm/520 nm for Cy2, 532 nm/580 nm for Cy3, and 633 nm/670 nm for Cy5) using a Typhoon 9400 laser scanner (GE Healthcare).

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