type iii collagenase dnase  (Millipore)


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    Name:
    Collagenase D
    Description:
    Collagenase D is prepared from Clostridium histolyticum cultures by filtration ammonium sulfate precipitation dialysis and lyophilization
    Catalog Number:
    colld-ro
    Price:
    None
    Applications:
    Collagenase from C. histolyticum is widely used for the disaggregation of many types of tissues (e.g., lung, heart, muscle, bone, adipose tissue, liver, kidney, cartilage, mammary gland, placentae, blood vessels, brain, tumors) and for the preparation of single cell suspensions for the establishment of primary cell culture systems. Collagenase D is recommended when functionality and integrity of cell-surface proteins are important.Clostridium collagenase from Roche has been used to prepare cells from many types of tissue, such as hepatocytes, adipocytes, pancreatic islets, epithelial cells, muscle cells, endothelial cells, etc. However, suitability of each lot of the enzyme for disruption of a particular tissue should be determined empirically.
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    Structured Review

    Millipore type iii collagenase dnase
    Collagenase D
    Collagenase D is prepared from Clostridium histolyticum cultures by filtration ammonium sulfate precipitation dialysis and lyophilization
    https://www.bioz.com/result/type iii collagenase dnase/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    type iii collagenase dnase - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Dysregulated Cytokine Expression by CD4+ T cells from Post-Septic Mice Modulates both Th1 and Th2-Mediated Granulomatous Lung Inflammation
    Article Snippet: .. Flow cytometry Collagenase-digested (Sigma-Aldrich, St. Louis, MO) lung lobes and undigested lymph nodes from animals were processed into single cell suspensions by processing tissues through sterile 40-mm filters, and ammonium chloride lysis buffer was used to eliminate erythrocytes. .. Cells were stained with the following fluorescent antibodies in flow cytometry buffer (phosphate buffered saline, 1% w/v bovine serum albumin, 0.05% w/v sodium azide): Purified αCD16/32 (Fc Block) (2.4G2, BD Biosciences, San Jose, CA), FITC-αCD8a (53–6.7, BD Biosciences), FITC-αCD3ε (145-2C11, BD Biosciences), PeCy7-αCD45 (Ly5, eBioscience, San Diego, CA), PeCy7-αCD3ε (17A2, BioLegend, San Diego, CA), Pacific Blue- (RM4-5, BioLegend) or Pacific Orange (RM4-5, Invitrogen, Carlsbad, CA) -αCD4, and Pacific Blue-αCD45 (30-F11, BioLegend).

    Isolation:

    Article Title: Lipopolysaccharide (LPS) Inhibits Steroid Production in Theca Cells of Bovine Follicles In Vitro: Distinct Effect of LPS on Theca Cell Function in Pre- and Post-selection Follicles
    Article Snippet: .. Culture of theca cells from PRFs and POFs Theca cells isolated from PRFs and POFs using the methods described above were placed in PBS containing 2 mg collagenase (452 U/mg, type 1, Sigma Chemical), 1 mg hyaluronidase (391 U/mg, type VIII, Sigma Chemical), 1 mg protease (4.5 U/g, Sigma Chemical) and 0.4% (v/v) bovine serum albumin, and a dissociation reaction was performed for 50 to 60 min at 37 C. Centrifugal separation was carried out at 350 × g . ..

    Article Title: Cooperative function of Pdx1 and Oc1 in multipotent pancreatic progenitors impacts postnatal islet maturation and adaptability
    Article Snippet: .. Isolation of islets from P14 pancreas required collagenase digestion of whole dissected pancreas at 37°C and hand-picking of islets away from exocrine tissue following a Histopaque-1077 (Sigma-Aldrich) gradient. ..

    Cytometry:

    Article Title: Dysregulated Cytokine Expression by CD4+ T cells from Post-Septic Mice Modulates both Th1 and Th2-Mediated Granulomatous Lung Inflammation
    Article Snippet: .. Flow cytometry Collagenase-digested (Sigma-Aldrich, St. Louis, MO) lung lobes and undigested lymph nodes from animals were processed into single cell suspensions by processing tissues through sterile 40-mm filters, and ammonium chloride lysis buffer was used to eliminate erythrocytes. .. Cells were stained with the following fluorescent antibodies in flow cytometry buffer (phosphate buffered saline, 1% w/v bovine serum albumin, 0.05% w/v sodium azide): Purified αCD16/32 (Fc Block) (2.4G2, BD Biosciences, San Jose, CA), FITC-αCD8a (53–6.7, BD Biosciences), FITC-αCD3ε (145-2C11, BD Biosciences), PeCy7-αCD45 (Ly5, eBioscience, San Diego, CA), PeCy7-αCD3ε (17A2, BioLegend, San Diego, CA), Pacific Blue- (RM4-5, BioLegend) or Pacific Orange (RM4-5, Invitrogen, Carlsbad, CA) -αCD4, and Pacific Blue-αCD45 (30-F11, BioLegend).

    Infection:

    Article Title: Resolution of a chronic viral infection after interleukin-10 receptor blockade
    Article Snippet: .. Splenocytes from mice infected 7 d earlier with LCMV Armstrong or LCMV clone 13 with or without anti–IL-10R antibody treatment were incubated with HBSS medium containing 0.5 mg/ml collagenase D (Sigma-Aldrich) at 37°C, 5% CO2 for 30 min. 0.01 M EDTA was added to disrupt T cell–DC complexes. .. Next, cells were depleted of CD3-expressing cells (Dynal CD3-beads; Dynal), incubated with CD11c microbeads (Miltenyi Biotec), and positively selected using MACS columns.

    Mouse Assay:

    Article Title: The MacBlue Binary Transgene (csf1r-gal4VP16/UAS-ECFP) Provides a Novel Marker for Visualisation of Subsets of Monocytes, Macrophages and Dendritic Cells and Responsiveness to CSF1 Administration
    Article Snippet: .. Liver FACS analysis Livers from Schedule 1 euthanized mice were digested in 2 mg/ml collagenase D (Sigma Aldrich) at 37°C for 30 min then passed through a 100 µm filter. .. Further purification of nonparenchyaml cells was performed using a 30% percoll (Sigma Aldrich) gradient.

    Article Title: Resolution of a chronic viral infection after interleukin-10 receptor blockade
    Article Snippet: .. Splenocytes from mice infected 7 d earlier with LCMV Armstrong or LCMV clone 13 with or without anti–IL-10R antibody treatment were incubated with HBSS medium containing 0.5 mg/ml collagenase D (Sigma-Aldrich) at 37°C, 5% CO2 for 30 min. 0.01 M EDTA was added to disrupt T cell–DC complexes. .. Next, cells were depleted of CD3-expressing cells (Dynal CD3-beads; Dynal), incubated with CD11c microbeads (Miltenyi Biotec), and positively selected using MACS columns.

    Incubation:

    Article Title: Resolution of a chronic viral infection after interleukin-10 receptor blockade
    Article Snippet: .. Splenocytes from mice infected 7 d earlier with LCMV Armstrong or LCMV clone 13 with or without anti–IL-10R antibody treatment were incubated with HBSS medium containing 0.5 mg/ml collagenase D (Sigma-Aldrich) at 37°C, 5% CO2 for 30 min. 0.01 M EDTA was added to disrupt T cell–DC complexes. .. Next, cells were depleted of CD3-expressing cells (Dynal CD3-beads; Dynal), incubated with CD11c microbeads (Miltenyi Biotec), and positively selected using MACS columns.

    Article Title: Polarization of macrophages in the tumor microenvironment is influenced by EGFR signaling within colon cancer cells
    Article Snippet: .. Briefly, colon tissues were cut into small pieces (1-2 mm) and incubated in 10 mL PRMI 1640 medium with 10 mM HEPES and 5% fetal bovine serum containing 10 mg (1 mg/mL) collagenase D (Sigma-Aldrich), 10 mg (1 mg/mL) dispase II (Roche, Germany), and 100 μL 10 mg/ml DNase I (100 μg/mL) (Sigma-Aldrich) for 30-45 min in a shaking incubator at 37°C. .. For Ana-1 and BMDM cells, single-cell suspensions from mouse colon tissues were incubated with APC-anti-mouse CD11b and FITC-anti-mouse F4/80 (Affymetrix eBioscience, USA), or APC-anti-mouse F4/80 and FITC-anti-mouse CD206 (MMR) (Biolegend, San Diego, CA) antibodies.

    FACS:

    Article Title: The MacBlue Binary Transgene (csf1r-gal4VP16/UAS-ECFP) Provides a Novel Marker for Visualisation of Subsets of Monocytes, Macrophages and Dendritic Cells and Responsiveness to CSF1 Administration
    Article Snippet: .. Liver FACS analysis Livers from Schedule 1 euthanized mice were digested in 2 mg/ml collagenase D (Sigma Aldrich) at 37°C for 30 min then passed through a 100 µm filter. .. Further purification of nonparenchyaml cells was performed using a 30% percoll (Sigma Aldrich) gradient.

    Lysis:

    Article Title: Dysregulated Cytokine Expression by CD4+ T cells from Post-Septic Mice Modulates both Th1 and Th2-Mediated Granulomatous Lung Inflammation
    Article Snippet: .. Flow cytometry Collagenase-digested (Sigma-Aldrich, St. Louis, MO) lung lobes and undigested lymph nodes from animals were processed into single cell suspensions by processing tissues through sterile 40-mm filters, and ammonium chloride lysis buffer was used to eliminate erythrocytes. .. Cells were stained with the following fluorescent antibodies in flow cytometry buffer (phosphate buffered saline, 1% w/v bovine serum albumin, 0.05% w/v sodium azide): Purified αCD16/32 (Fc Block) (2.4G2, BD Biosciences, San Jose, CA), FITC-αCD8a (53–6.7, BD Biosciences), FITC-αCD3ε (145-2C11, BD Biosciences), PeCy7-αCD45 (Ly5, eBioscience, San Diego, CA), PeCy7-αCD3ε (17A2, BioLegend, San Diego, CA), Pacific Blue- (RM4-5, BioLegend) or Pacific Orange (RM4-5, Invitrogen, Carlsbad, CA) -αCD4, and Pacific Blue-αCD45 (30-F11, BioLegend).

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  • 99
    Millipore collagenase dnase milliporesigma solution
    Collagenase Dnase Milliporesigma Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase dnase milliporesigma solution/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase dnase milliporesigma solution - by Bioz Stars, 2020-09
    99/100 stars
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