Structured Review

GE Healthcare ttbs
Ttbs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 506 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ttbs/product/GE Healthcare
Average 94 stars, based on 506 article reviews
Price from $9.99 to $1999.99
ttbs - by Bioz Stars, 2020-07
94/100 stars

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Related Articles

Blocking Assay:

Article Title: Chronic alcohol ingestion exacerbates skeletal muscle myopathy in HIV-1 transgenic rats
Article Snippet: .. Blots were washed in TTBS, incubated in anti-rabbit-HRP IgG (1;2500 in blocking buffer) for 1 h, washed again and then developed with enhanced chemiluminescent plus western blotting detection system (GE Healthcare, Piscataway, NJ). .. Densitometry was performed using a Chemidoc XRS system and analyzed with Quantity One software (Biorad, Hercules, CA).

Incubation:

Article Title: Chronic alcohol ingestion exacerbates skeletal muscle myopathy in HIV-1 transgenic rats
Article Snippet: .. Blots were washed in TTBS, incubated in anti-rabbit-HRP IgG (1;2500 in blocking buffer) for 1 h, washed again and then developed with enhanced chemiluminescent plus western blotting detection system (GE Healthcare, Piscataway, NJ). .. Densitometry was performed using a Chemidoc XRS system and analyzed with Quantity One software (Biorad, Hercules, CA).

Article Title: Lack of collagen VI promotes neurodegeneration by impairing autophagy and inducing apoptosis during aging
Article Snippet: .. Membranes were then washed three times with TTBS, and incubated for 1 hr at room temperature with HRP-conjugated secondary antibodies (Amersham Bioscience) in TTBS supplemented with 2.5% milk. .. Detection was performed by chemiluminescence (Pierce).

Article Title: Anti-arrhythmic Cardiac Phenotype Elicited by Chronic Intermittent Hypoxia Is Associated With Alterations in Connexin-43 Expression, Phosphorylation, and Distribution
Article Snippet: .. Subsequently, membranes were washed in TTBS and incubated with the appropriate secondary antibodies: either anti-rabbit (GE Healthcare Amersham) or anti-mouse immunoglobulins (Thermo Fisher Scientific) conjugated with HRP, visualized by chemiluminescence (DuraSignal) using LAS-4000 imaging system (Genetica, Fujifilm) and quantified using Quantity One Software (Bio-Rad). .. Glyceraldehyde 3-phosphate dehydrogenase and actin expression was used as loading control.

Article Title: Ligand-independent Dimer Formation of Epidermal Growth Factor Receptor (EGFR) Is a Step Separable from Ligand-induced EGFR Signaling
Article Snippet: .. The membrane was blocked with 3% skim milk in TBS (20 mM Tris, 0.1 M NaCl, pH 7.5) at 37°C for 1 h, then incubated with primary antibody in TBS containing 1% skim milk at room temperature for 1 h. Next, the membrane was washed four times with TTBS (TBS containing 0.05% Tween 20), incubated with HRP-conjugated secondary antibody, and finally analyzed with an ECL-Western blotting kit (Amersham International plc, Buckinghamshire, England). .. Cells were seeded into 24-well plates at a density of 5 × 104 cells/well with or without each EGF ligand in fresh RPMI 1640 medium containing 10% serum, then cultured at 37°C for 24 h and incubated with [3 H]thymidine (37 kBq/ml) for 4 h. Cells were harvested, and radioactivity incorporated into DNA was determined as described previously ( ).

Imaging:

Article Title: Anti-arrhythmic Cardiac Phenotype Elicited by Chronic Intermittent Hypoxia Is Associated With Alterations in Connexin-43 Expression, Phosphorylation, and Distribution
Article Snippet: .. Subsequently, membranes were washed in TTBS and incubated with the appropriate secondary antibodies: either anti-rabbit (GE Healthcare Amersham) or anti-mouse immunoglobulins (Thermo Fisher Scientific) conjugated with HRP, visualized by chemiluminescence (DuraSignal) using LAS-4000 imaging system (Genetica, Fujifilm) and quantified using Quantity One Software (Bio-Rad). .. Glyceraldehyde 3-phosphate dehydrogenase and actin expression was used as loading control.

Planar Chromatography:

Article Title: Ligand-independent Dimer Formation of Epidermal Growth Factor Receptor (EGFR) Is a Step Separable from Ligand-induced EGFR Signaling
Article Snippet: .. The membrane was blocked with 3% skim milk in TBS (20 mM Tris, 0.1 M NaCl, pH 7.5) at 37°C for 1 h, then incubated with primary antibody in TBS containing 1% skim milk at room temperature for 1 h. Next, the membrane was washed four times with TTBS (TBS containing 0.05% Tween 20), incubated with HRP-conjugated secondary antibody, and finally analyzed with an ECL-Western blotting kit (Amersham International plc, Buckinghamshire, England). .. Cells were seeded into 24-well plates at a density of 5 × 104 cells/well with or without each EGF ligand in fresh RPMI 1640 medium containing 10% serum, then cultured at 37°C for 24 h and incubated with [3 H]thymidine (37 kBq/ml) for 4 h. Cells were harvested, and radioactivity incorporated into DNA was determined as described previously ( ).

Western Blot:

Article Title: Dopamine and ?-synuclein dysfunction in Smad3 null mice
Article Snippet: .. After rinsing in TTBS, the reaction was visualized using the ECL Advance Western Blotting Detection Kit (Amersham). .. Images were captured using VersaDoc Imaging System (Model 4000, Bio-Rad), and the band densities were quantified with ImageQuant TL software (Amersham).

Article Title: Chronic alcohol ingestion exacerbates skeletal muscle myopathy in HIV-1 transgenic rats
Article Snippet: .. Blots were washed in TTBS, incubated in anti-rabbit-HRP IgG (1;2500 in blocking buffer) for 1 h, washed again and then developed with enhanced chemiluminescent plus western blotting detection system (GE Healthcare, Piscataway, NJ). .. Densitometry was performed using a Chemidoc XRS system and analyzed with Quantity One software (Biorad, Hercules, CA).

Article Title: The Depletion of Nuclear Glutathione Impairs Cell Proliferation in 3t3 Fibroblasts
Article Snippet: .. Finally, blots were washed during 5 minutes three times with TTBS and detection was carried out using ECL Western blotting detection reagent (Amersham, GE HealthcareBio-Sciences AB, Uppsala, Sweden). ..

Software:

Article Title: Anti-arrhythmic Cardiac Phenotype Elicited by Chronic Intermittent Hypoxia Is Associated With Alterations in Connexin-43 Expression, Phosphorylation, and Distribution
Article Snippet: .. Subsequently, membranes were washed in TTBS and incubated with the appropriate secondary antibodies: either anti-rabbit (GE Healthcare Amersham) or anti-mouse immunoglobulins (Thermo Fisher Scientific) conjugated with HRP, visualized by chemiluminescence (DuraSignal) using LAS-4000 imaging system (Genetica, Fujifilm) and quantified using Quantity One Software (Bio-Rad). .. Glyceraldehyde 3-phosphate dehydrogenase and actin expression was used as loading control.

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    GE Healthcare ttbs
    Ttbs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 506 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ttbs/product/GE Healthcare
    Average 94 stars, based on 506 article reviews
    Price from $9.99 to $1999.99
    ttbs - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    91
    GE Healthcare ttbs buffer
    Ttbs Buffer, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ttbs buffer/product/GE Healthcare
    Average 91 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    ttbs buffer - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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