Structured Review

Cell Signaling Technology Inc ttbs
Ttbs, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ttbs/product/Cell Signaling Technology Inc
Average 93 stars, based on 15 article reviews
Price from $9.99 to $1999.99
ttbs - by Bioz Stars, 2020-07
93/100 stars

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Western Blot:

Article Title: Upregulation of calcium-sensing receptor and mitogen-activated protein kinase signalling in the regulation of growth and differentiation in colon carcinoma
Article Snippet: .. Thereafter, the membranes were washed with TTBS and bound antibody detected using the Phototope-HRP Western blot detection kit (Cell Signaling Technologies Inc.). .. Images were scanned, digitised and quantified using NIH image analysis software.

Article Title: IL-17 induces production of IL-6 and IL-8 in rheumatoid arthritis synovial fibroblasts via NF-?B- and PI3-kinase/Akt-dependent pathways
Article Snippet: .. For western hybridization, the membrane was pre-incubated with 0.1% skimmed milk in TTBS (0.1% Tween 20 in Tris-buffered saline) at room temperature for 2 hours; then primary antibodies to either Akt or phosphorylated Akt (Cell Signaling Technology Inc, Beverly, MA, USA), diluted 1 : 200 in PBS, were added and incubated for 1 hour at room temperature. .. After the preparations had been washed 4 times with TTBS, horseradish-peroxidase-conjugated secondary antibodies (Amersham Pharmacia) were added and allowed to incubate for 30 min at room temperature.

Incubation:

Article Title: IL-22 Negatively Regulates Helicobacter pylori-Induced CCL20 Expression in Gastric Epithelial Cells
Article Snippet: .. The membranes were blocked with 5% milk in TTBS (150 mM NaCl, 100 mM Tris pH 7.5, 0.1% Tween 20) for 1 h before incubated with anti-phosho-STAT3 (Cell signal Technology, Beverly, MA) at 4°C overnight, followed by three washes. .. The membrane was then incubated with peroxidase conjugated goat anti-rabbit antibody for 1 h at room temperature followed by three washes, and finally subjected to chemiluminescence using the ECL reagent (Perkin Elmer, Waltham, MA).

Article Title: A novel curcumin analog inhibits canonical and non-canonical functions of telomerase through STAT3 and NF-κB inactivation in colorectal cancer cells
Article Snippet: .. The membrane was blocked for 1 hour in 5% skim milk at 4° C. The membrane was then washed 3 times with 1x TTBS and incubated overnight with the primary antibody at 4° C. Primary antibodies of CD44, Oct-4, ALDHA1, STAT3, pSTAT3, pNF-κB, NF-κB and β-actin were purchased from Cell Signaling Technology (Danvers, MA). .. After incubation with the secondary antibodies conjugated with horseradish peroxidase (HRP), the protein bands were developed with the chemiluminescent reagents.

Article Title: Fluoxetine normalizes disrupted light-induced entrainment, fragmented ultradian rhythms and altered hippocampal clock gene expression in an animal model of high trait anxiety- and depression-related behavior
Article Snippet: .. Membranes were then incubated with a diluted primary antibody (Per2 and Per3 (Alpha Diagnostic International Inc, TX) [1:1000], Cry2 (Abcam, Plc, Cambridge, UK) [1:500]) overnight at 4 °C, rinsed three times with TTBS, and incubated for 1h at room temperature with a horseradish peroxidase-conjugated secondary antibody (Goat Anti-rabbit HRP-linked IgG, Cell Signaling Technology, Inc., Danvers, MA [1:3000]). .. Immunoreactivity was visualized by enhanced chemiluminescence Pierce ECL substrate (Thermo Scientific, Vienna, Austria).

Article Title: IL-1ra Secreted by ATP-Induced P2Y2 Negatively Regulates MUC5AC Overproduction via PLCβ3 during Airway Inflammation
Article Snippet: .. After washing with TTBS, blots were further incubated for 45 min at room temperature with anti-rabbit or anti-mouse antibody (Cell Signaling; Danvers, MA) in TTBS and visualized using the ECL system (GE Healthcare; Uppsala, Sweden). .. Measurement of Intracellular Ca2+ Concentration ([Ca2+ ]i ) NCI-H292 cells were seeded on cover glasses in 35-mm dishes (5 × 104 cells).

Article Title: Chronic alcohol ingestion delays skeletal muscle regeneration following injury
Article Snippet: .. Membranes were blocked in 5% BSA diluted in TTBS (0.01% (w/v) Tween-20) for 1 h and then incubated in primary antibodies against collagen type VI (Cell Signaling Technology, Beverly, MA; 1:1000 in blocking buffer), fibronectin and actin (Santa Cruz Biotechnology Inc., Santa Cruz, CA; 1:250 in blocking buffer) overnight at 4°C. .. Blots were washed in TTBS, incubated in anti-rabbit-HRP IgG (1;2500 in blocking buffer) for 1 h, washed again and then developed with enhanced chemiluminescent plus western blotting detection system (GE Healthcare, Piscataway, NJ).

Article Title: IL-17 induces production of IL-6 and IL-8 in rheumatoid arthritis synovial fibroblasts via NF-?B- and PI3-kinase/Akt-dependent pathways
Article Snippet: .. For western hybridization, the membrane was pre-incubated with 0.1% skimmed milk in TTBS (0.1% Tween 20 in Tris-buffered saline) at room temperature for 2 hours; then primary antibodies to either Akt or phosphorylated Akt (Cell Signaling Technology Inc, Beverly, MA, USA), diluted 1 : 200 in PBS, were added and incubated for 1 hour at room temperature. .. After the preparations had been washed 4 times with TTBS, horseradish-peroxidase-conjugated secondary antibodies (Amersham Pharmacia) were added and allowed to incubate for 30 min at room temperature.

Blocking Assay:

Article Title: Chronic alcohol ingestion delays skeletal muscle regeneration following injury
Article Snippet: .. Membranes were blocked in 5% BSA diluted in TTBS (0.01% (w/v) Tween-20) for 1 h and then incubated in primary antibodies against collagen type VI (Cell Signaling Technology, Beverly, MA; 1:1000 in blocking buffer), fibronectin and actin (Santa Cruz Biotechnology Inc., Santa Cruz, CA; 1:250 in blocking buffer) overnight at 4°C. .. Blots were washed in TTBS, incubated in anti-rabbit-HRP IgG (1;2500 in blocking buffer) for 1 h, washed again and then developed with enhanced chemiluminescent plus western blotting detection system (GE Healthcare, Piscataway, NJ).

Hybridization:

Article Title: IL-17 induces production of IL-6 and IL-8 in rheumatoid arthritis synovial fibroblasts via NF-?B- and PI3-kinase/Akt-dependent pathways
Article Snippet: .. For western hybridization, the membrane was pre-incubated with 0.1% skimmed milk in TTBS (0.1% Tween 20 in Tris-buffered saline) at room temperature for 2 hours; then primary antibodies to either Akt or phosphorylated Akt (Cell Signaling Technology Inc, Beverly, MA, USA), diluted 1 : 200 in PBS, were added and incubated for 1 hour at room temperature. .. After the preparations had been washed 4 times with TTBS, horseradish-peroxidase-conjugated secondary antibodies (Amersham Pharmacia) were added and allowed to incubate for 30 min at room temperature.

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    Cell Signaling Technology Inc ttbs
    Ttbs, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ttbs/product/Cell Signaling Technology Inc
    Average 93 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    ttbs - by Bioz Stars, 2020-07
    93/100 stars
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