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Becton Dickinson ttbs
Ttbs, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ttbs/product/Becton Dickinson
Average 92 stars, based on 17 article reviews
Price from $9.99 to $1999.99
ttbs - by Bioz Stars, 2020-07
92/100 stars

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Incubation:

Article Title: Listeriolysin O Causes ENaC Dysfunction in Human Airway Epithelial Cells
Article Snippet: .. After being blocked for 1 h at room temperature in 1% BSA buffer dissolved in TTBS (TBS with 0.1% Tween 20), the membranes were incubated overnight with purified mouse anti-human PKC-α antibodies (all from BD Transduction, Lexington, KY, USA). .. Subsequently, nitrocellulose membranes were washed three times for 10 min each with TTBS and incubated with the appropriate secondary antibody (Amersham, Arlington Heights, IL, USA).

Article Title: Methylalpinumisoflavone Inhibits Hypoxia-inducible Factor-1 (HIF-1) Activation by Simultaneously Targeting Multiple Pathways
Article Snippet: .. The membrane was blocked in 5% nonfat milk (Bio-Rad) in TTBS (20 m m Tris, pH 6.8, 150 m m NaCl, 0.05% Tween 20) at 4 °C overnight and incubated with specified primary antibodies in TTBS with 1% bovine serum albumin at room temperature for 1 h. Both monoclonal anti-HIF-1α antibody (1:500) and monoclonal anti-HIF-1β antibody (1:1000) were from BD Biosciences. ..

Article Title: Comparison of mechanisms involved in impaired vascular reactivity between high sucrose and high fat diets in rats
Article Snippet: .. Following washes in TTBS, PVDF membranes were incubated overnight at 4˚C with mouse monoclonal antibody specific for eNOS (1:2500; Cat. 610296; BD Transduction Laboratories, San Jose, CA) and rabbit polyclonal antibody to β-actin as a loading control (1:10,000; Cat. Ab8227; AbCam, Cambridge, MA). .. Membranes were then washed in TTBS and incubated with anti-mouse and anti-rabbit horseradish peroxidase-conjugated secondary antibodies (1:5000, Cat. PI-2000 and PI-1000; Vector Laboratories, Burlingame, CA) for 1 hr at room temperature followed by washes in Tris-buffered saline (TBS) and a 1 min exposure to Pierce enhanced chemiluminescence western blotting substrate (Thermo Scientific, Rockford, IL).

Article Title: Proteomics Analysis of Epithelial Cells Reprogrammed in Cell-free Extract *Proteomics Analysis of Epithelial Cells Reprogrammed in Cell-free Extract * S⃞
Article Snippet: .. After four washes with TTBS, membranes were incubated in a goat anti-mouse horseradish peroxidase-conjugated secondary antibody (catalogue number 554002, BD Biosciences) for 1.5 h (1:1000 dilution). .. After four more washes with TTBS, immunoreactive proteins on the membranes were detected using SuperSignal West Dura chemiluminescent reagents (Perbio) and exposure to Hyperfilm ECL high performance chemiluminescent film (GE Healthcare).

Article Title: The c-Abl inhibitor, Radotinib HCl, is neuroprotective in a preclinical Parkinson’s disease mouse model
Article Snippet: .. Membrane was blocked with TTBS (150 mM NaCl, 10 mM Tris–HCl (pH 7.4), 0.05% Tween 20) containing 5% skim milk for 30–60 min and incubated at 4°C overnight with primary antibodies; mouse anti-α-synuclein (610787; BD Transduction Laboratories), rabbit anti-pY245 c-Abl (2861; Cell Signaling Technology), mouse anti-c-Abl (554148; BD Transduction Laboratories), rabbit monoclonal anti-pS129 α-synuclein (ab168381; Abcam), rabbit polyclonal anti-phospho-p38 MAPK (Thr180/Tyr182) (9211; Cell Signaling Technology), rabbit polyclonal anti-p38 MAPK ( ; Abclonal Technology) followed by HRP-conjugated rabbit or mouse secondary antibodies (1: 5000, GE Healthcare) for 1 h at room temperature. .. Rabbit polyclonal anti-pY39-α-synuclein antibody was generated as described previously ( ).

Article Title: The lectin-like domain of TNF protects from listeriolysin-induced hyperpermeability in human pulmonary microvascular endothelial cells -- A crucial role for protein kinase C-? inhibition
Article Snippet: .. After being blocked for 1 h at room temperature in 1% BSA buffer dissolved in TTBS (TBS with 0.1% Tween 20), the membranes were incubated overnight with purified mouse anti-human PKCα antibodies (all from BD Transduction, Lexington, KY) and β-actin antibody (Santa Cruz Biotechnology, Santa Cruz, CA). .. Subsequently, nitrocellulose membranes were washed three times for 10 min each with TTBS and incubated with appropriate secondary antibodies (Amersham, Arlington Heights, IL).

SDS Page:

Article Title: Maternal Microchimerism Leads to the Presence of Interleukin-2 in Interleukin-2 Knock Out Mice: Implications for the Role of Interleukin-2 in Thymic Function
Article Snippet: .. The thymic extracts were separated by SDS-PAGE on 4–20% gradient gels (Bio-Rad), transferred to 0.2 μm nitrocellulose, blocked with TTBS (Tris-buffered saline with 0.1% Tween-20) then probed for 16 hours with a rat anti-mouse IL-2 antibody (clone 18161D, BD Pharmingen). ..

Purification:

Article Title: Listeriolysin O Causes ENaC Dysfunction in Human Airway Epithelial Cells
Article Snippet: .. After being blocked for 1 h at room temperature in 1% BSA buffer dissolved in TTBS (TBS with 0.1% Tween 20), the membranes were incubated overnight with purified mouse anti-human PKC-α antibodies (all from BD Transduction, Lexington, KY, USA). .. Subsequently, nitrocellulose membranes were washed three times for 10 min each with TTBS and incubated with the appropriate secondary antibody (Amersham, Arlington Heights, IL, USA).

Article Title: The lectin-like domain of TNF protects from listeriolysin-induced hyperpermeability in human pulmonary microvascular endothelial cells -- A crucial role for protein kinase C-? inhibition
Article Snippet: .. After being blocked for 1 h at room temperature in 1% BSA buffer dissolved in TTBS (TBS with 0.1% Tween 20), the membranes were incubated overnight with purified mouse anti-human PKCα antibodies (all from BD Transduction, Lexington, KY) and β-actin antibody (Santa Cruz Biotechnology, Santa Cruz, CA). .. Subsequently, nitrocellulose membranes were washed three times for 10 min each with TTBS and incubated with appropriate secondary antibodies (Amersham, Arlington Heights, IL).

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