tsa plus fluorescence system  (PerkinElmer)

 
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    Name:
    TSA Plus Fluorescein System
    Description:
    TSA Plus Fluorescence Systems Signal amplification for IHC ISH and related applications Direct fluorescence detection Multi fluor combination kits for convenient multi target detection 10 to 20 times higher sensitivity than regular TSA Conserve precious antibodies or probes High resolution results Adds 2 steps to existing protocol for rapid results
    Catalog Number:
    nel741001kt
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    None
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    1 each
    Category:
    Reagents
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    Structured Review

    PerkinElmer tsa plus fluorescence system
    TSA Plus Fluorescein System
    TSA Plus Fluorescence Systems Signal amplification for IHC ISH and related applications Direct fluorescence detection Multi fluor combination kits for convenient multi target detection 10 to 20 times higher sensitivity than regular TSA Conserve precious antibodies or probes High resolution results Adds 2 steps to existing protocol for rapid results
    https://www.bioz.com/result/tsa plus fluorescence system/product/PerkinElmer
    Average 99 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    tsa plus fluorescence system - by Bioz Stars, 2020-04
    99/100 stars

    Images

    1) Product Images from "A Pheromone Receptor Mediates 11-cis-Vaccenyl Acetate-Induced Responses in Drosophila"

    Article Title: A Pheromone Receptor Mediates 11-cis-Vaccenyl Acetate-Induced Responses in Drosophila

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.0876-06.2006

    Expression of Or67d is defective in tod 1 and tot 1 mutants. A , Schematic diagrams of Or83b and Or67d gene fragments and the relative location of the PCR primers. Primers were chosen to span an intron to discriminate genomic contamination from cDNA products. The thick line denotes the exon sequences, and the thin line denotes the intron region of each gene fragment. The expected cDNA size of the RT-PCR products was 296 bp for Or67d and 269 bp for Or83b. The expected genomic DNA size of the RT-PCR products was 368 bp for Or67d and 630 bp for Or83b. The arrows indicate priming sites for each specific primer sets. B , Or67d in tod 1 and tot 1 mutants flies is absent or dramatically reduced (long arrow). Or83b was used as a positive control for RNA integrity and RT quality (short arrow). C , RNA in situ hybridization with antisense probes to Or83b (left panels; red colors) and Or67d (right panels; green colors) in w 1118 , tod 1 , and tot 1 mutants. The bottom panel shows antisense labeling in antennas misexpressing Or67d under control of the ELAV promoter. Or83 and Or67d signals were amplified with TSA-Plus Cy5 (red) or TSA-Plus Fluorescence systems (green).
    Figure Legend Snippet: Expression of Or67d is defective in tod 1 and tot 1 mutants. A , Schematic diagrams of Or83b and Or67d gene fragments and the relative location of the PCR primers. Primers were chosen to span an intron to discriminate genomic contamination from cDNA products. The thick line denotes the exon sequences, and the thin line denotes the intron region of each gene fragment. The expected cDNA size of the RT-PCR products was 296 bp for Or67d and 269 bp for Or83b. The expected genomic DNA size of the RT-PCR products was 368 bp for Or67d and 630 bp for Or83b. The arrows indicate priming sites for each specific primer sets. B , Or67d in tod 1 and tot 1 mutants flies is absent or dramatically reduced (long arrow). Or83b was used as a positive control for RNA integrity and RT quality (short arrow). C , RNA in situ hybridization with antisense probes to Or83b (left panels; red colors) and Or67d (right panels; green colors) in w 1118 , tod 1 , and tot 1 mutants. The bottom panel shows antisense labeling in antennas misexpressing Or67d under control of the ELAV promoter. Or83 and Or67d signals were amplified with TSA-Plus Cy5 (red) or TSA-Plus Fluorescence systems (green).

    Techniques Used: Expressing, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Positive Control, RNA In Situ Hybridization, Labeling, Amplification, Fluorescence

    2) Product Images from "HCV Induces Oxidative and ER Stress, and Sensitizes Infected Cells to Apoptosis in SCID/Alb-uPA Mice"

    Article Title: HCV Induces Oxidative and ER Stress, and Sensitizes Infected Cells to Apoptosis in SCID/Alb-uPA Mice

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1000291

    Confocal microscopic analysis of HCV antigen expression and either FAS expression or TUNEL reactivity. Staining for FAS (A) or TUNEL reactivity (B) was performed as described in Materials and Methods , using primary mouse anti-HCV and either rabbit anti-human FAS (A), or for TUNEL, FITC labeled dUTP and terminal deoxynucleotidyl transferase (B). The secondary antibodies were goat anti mouse poly-HRP, and for FAS staining, goat anti rabbit Alexa-488 (green). HRP was developed using the TSA plus fluorescence system with tyramide-TMR (red). Nuclei were stained using DAPI (blue). Arrows indicate TUNEL reactive nuclei.
    Figure Legend Snippet: Confocal microscopic analysis of HCV antigen expression and either FAS expression or TUNEL reactivity. Staining for FAS (A) or TUNEL reactivity (B) was performed as described in Materials and Methods , using primary mouse anti-HCV and either rabbit anti-human FAS (A), or for TUNEL, FITC labeled dUTP and terminal deoxynucleotidyl transferase (B). The secondary antibodies were goat anti mouse poly-HRP, and for FAS staining, goat anti rabbit Alexa-488 (green). HRP was developed using the TSA plus fluorescence system with tyramide-TMR (red). Nuclei were stained using DAPI (blue). Arrows indicate TUNEL reactive nuclei.

    Techniques Used: Expressing, TUNEL Assay, Staining, Labeling, Fluorescence

    Related Articles

    Amplification:

    Article Title: Inhibition of ErbB kinase signalling promotes resolution of neutrophilic inflammation
    Article Snippet: .. TSA signal amplification of GFP-labelled neutrophils (driven by endogenous peroxidase activity) was carried out using TSA Plus Fluorescein System (Perkin Elmer). ..

    Article Title: The Nutrient-Responsive Hormone CCHamide-2 Controls Growth by Regulating Insulin-like Peptides in the Brain of Drosophila melanogaster
    Article Snippet: Digoxigenin-labeled RNA probes were detected using the TSA Plus Fluorescein Kit (PerkinElmer). .. For the detection of CCHa2-R mRNA, the signals were further amplified using mouse anti-FITC (1:1,000; Jackson ImmunoResearch) and anti-mouse-Alexa 488 antibodies.

    Article Title: A Pheromone Receptor Mediates 11-cis-Vaccenyl Acetate-Induced Responses in Drosophila
    Article Snippet: .. Signals were amplified with the TSA-Plus Cyanine 5 system (PerkinElmer, Wellesley, MA) for Or83b or the TSA-Plus Fluorescence system (PerkinElmer) for Or67d ( ). .. A cDNA encoding Or67d was obtained by reverse transcription, PCR amplification of RNA isolated from antenna of Drosophila w1118 , a strain known to be responsive to VA pheromone (our unpublished observation) (see Figs. 1, 4).

    Neutralization:

    Article Title: The long noncoding RNA Vax2os1 controls the cell cycle progression of photoreceptor progenitors in the mouse retina
    Article Snippet: The cryopreserved sections were permeabilized with 0.5% Triton X-100 in PBS and blocked with 10% goat serum in PBS/0.2% Triton X-100 for 30 min. For the BrdU and Ki67 staining, sections were boiled, after fixation, in 10 mM sodium citrate buffer (pH 6.0) for 3 min. An additional denaturation step with 2 N chloridric acid for 30 min, and neutralization in PBS was performed for the BrdU detection. .. The ABC kit (Vector Laboratories) and TSA-plus fluorescein (Perkin Elmer) were used according to the manufacturer's instructions to amplify the signal in Ki67 staining on eye sections.

    Blocking Assay:

    Article Title: HCV Induces Oxidative and ER Stress, and Sensitizes Infected Cells to Apoptosis in SCID/Alb-uPA Mice
    Article Snippet: Additional blocking using mouse IgG (0.1 mg/ml) for 1 hour, followed by incubation with goat anti mouse-IgG (1 mg/ml) overnight at 4°C was done prior to incubation with mouse anti HCV NS3/4 diluted 1∶50 (TORDJI-22, Abcam), or its isotype control, and one of rabbit anti-FAS, BAX, GADD (Santa Cruz), NF-κB p65 (C-20 Santa Cruz), BCL-xL (Cell Signaling Technologies), or rabbit IgG all diluted 1∶50, or rabbit anti human Albumin diluted 1∶1000 (Dako Cytomation). .. The peroxidase was developed using the TSA Plus fluorescence system with tyramide-tetramethyl red (Perkin Elmer).

    Article Title: Defining BMP functions in the hair follicle by conditional ablation of BMP receptor IA
    Article Snippet: In other cases, the following block/diluent was used 2.5% NDS, 2.5% NGS, 1% BSA, 2% gelatin, and 1% Triton X-100 in PBS. .. For phospho-Smad1, we used the TSA™ Plus Fluorescing System from Perkin Elmer Life Sciences.

    Article Title: Rhythmic Trafficking of TRPV2 in the Suprachiasmatic Nucleus is Regulated by Prokineticin 2 Signaling
    Article Snippet: Sections were incubated with an anti-DIG antibody conjugated with horseradish peroxidase (Perkin Elmer) in blocking solution overnight at 4°C and washed with PBST. .. DIG labeled cells were revealed with the TSA Plus Fluorescence Kit from Perkin Elmer as described in the instruction manual.

    Incubation:

    Article Title: HCV Induces Oxidative and ER Stress, and Sensitizes Infected Cells to Apoptosis in SCID/Alb-uPA Mice
    Article Snippet: Slides were blocked with 3% peroxide prior to incubation with goat anti mouse poly HRP and goat anti rabbit Alexa 488 (diluted 1∶100, Molecular Probes, Eugene, OR, USA). .. The peroxidase was developed using the TSA Plus fluorescence system with tyramide-tetramethyl red (Perkin Elmer).

    Article Title: The long noncoding RNA Vax2os1 controls the cell cycle progression of photoreceptor progenitors in the mouse retina
    Article Snippet: For the immunofluorescence analysis on the eye sections, mouse eyes were fixed overnight in 4% paraformaldehyde in PBS at 4°C, incubated for 1 h in 20% sucrose/PBS, and incubated overnight in 30% sucrose/PBS at 4°C. .. The ABC kit (Vector Laboratories) and TSA-plus fluorescein (Perkin Elmer) were used according to the manufacturer's instructions to amplify the signal in Ki67 staining on eye sections.

    Article Title: Rhythmic Trafficking of TRPV2 in the Suprachiasmatic Nucleus is Regulated by Prokineticin 2 Signaling
    Article Snippet: Sections were incubated with an anti-DIG antibody conjugated with horseradish peroxidase (Perkin Elmer) in blocking solution overnight at 4°C and washed with PBST. .. DIG labeled cells were revealed with the TSA Plus Fluorescence Kit from Perkin Elmer as described in the instruction manual.

    Activity Assay:

    Article Title: Inhibition of ErbB kinase signalling promotes resolution of neutrophilic inflammation
    Article Snippet: .. TSA signal amplification of GFP-labelled neutrophils (driven by endogenous peroxidase activity) was carried out using TSA Plus Fluorescein System (Perkin Elmer). ..

    Expressing:

    Article Title: Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments
    Article Snippet: The total Sox4 expression score corresponds with the sum of the SN and SE scores. .. Triple immunofluorescence staining was performed with CXCL13 antibody (BCA1, rabbit polyclonal, Thermo Fisher, 1:100), Sox4 (HPA029901, rabbit polyclonal, Sigma Aldrich, 1:30), CD3 (Clone PS1, mouse monoclonal, Leica Microsystems, 1:200), CD4 (Clone 1F6, mouse monoclonal, Leica Microsystems, 1:100), CXCR5 (Clone 51505, mouse monoclonal, R & D Systems, 1:1000), and PD-1 (Clone NAT105, mouse monoclonal, Abcam, 1:50) and detected with TSA Plus Fluorescence Kit (PerkinElmer, Inc.).

    Article Title: A Pheromone Receptor Mediates 11-cis-Vaccenyl Acetate-Induced Responses in Drosophila
    Article Snippet: Expression of Or67d in control, tot1 , and tod1 mutants were preformed as described previously , with modification for fluorescent probes as described by . .. Signals were amplified with the TSA-Plus Cyanine 5 system (PerkinElmer, Wellesley, MA) for Or83b or the TSA-Plus Fluorescence system (PerkinElmer) for Or67d ( ).

    Modification:

    Article Title: The Nutrient-Responsive Hormone CCHamide-2 Controls Growth by Regulating Insulin-like Peptides in the Brain of Drosophila melanogaster
    Article Snippet: FISH was performed as described in Lehmann and Tautz [ ] with a modification designed to amplify signals. .. Digoxigenin-labeled RNA probes were detected using the TSA Plus Fluorescein Kit (PerkinElmer).

    Article Title: A Pheromone Receptor Mediates 11-cis-Vaccenyl Acetate-Induced Responses in Drosophila
    Article Snippet: Expression of Or67d in control, tot1 , and tod1 mutants were preformed as described previously , with modification for fluorescent probes as described by . .. Signals were amplified with the TSA-Plus Cyanine 5 system (PerkinElmer, Wellesley, MA) for Or83b or the TSA-Plus Fluorescence system (PerkinElmer) for Or67d ( ).

    Hybridization:

    Article Title: Organ-specific requirements for Hdac1 in liver and pancreas formation
    Article Snippet: Paragraph title: In situ hybridisation ... Fluorescent in situ hybridisations were carried out using the TSA Plus Fluorescence palette system (Perkin-Elmer).

    Article Title: Rhythmic Trafficking of TRPV2 in the Suprachiasmatic Nucleus is Regulated by Prokineticin 2 Signaling
    Article Snippet: Sections were pretreated with proteinase K (10 µg/ml) and pre-hybridized with the hybridization solution without probe for 1 hr at room temperature. .. DIG labeled cells were revealed with the TSA Plus Fluorescence Kit from Perkin Elmer as described in the instruction manual.

    TUNEL Assay:

    Article Title: Inhibition of ErbB kinase signalling promotes resolution of neutrophilic inflammation
    Article Snippet: TSA signal amplification of GFP-labelled neutrophils (driven by endogenous peroxidase activity) was carried out using TSA Plus Fluorescein System (Perkin Elmer). .. Larvae were post-fixed in 4% paraformaldehyde, before subsequent TUNEL staining for apoptosis using ApopTag Red In Situ Apoptosis Detection Kit (Millipore).

    Article Title: HCV Induces Oxidative and ER Stress, and Sensitizes Infected Cells to Apoptosis in SCID/Alb-uPA Mice
    Article Snippet: The peroxidase was developed using the TSA Plus fluorescence system with tyramide-tetramethyl red (Perkin Elmer). .. For co-localization of HCV antigens and TUNEL reactivity, the In Situ Cell Death Detection kit (fluorescein) (Roche) was used, according to the manufacturer's specifications.

    Immunohistochemistry:

    Article Title: Increased B Cell ADAM10 in Allergic Patients and Th2 Prone Mice
    Article Snippet: Paragraph title: Immunohistochemistry and confocal microscopy ... TNF labeling was enhanced using Fluorescein-TSA (TSA Plus Fluorescein System, Perkin Elmer, NEL741001KT) [ ].

    Article Title: Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments
    Article Snippet: Paragraph title: Immunohistochemistry and immunofluorescence ... Triple immunofluorescence staining was performed with CXCL13 antibody (BCA1, rabbit polyclonal, Thermo Fisher, 1:100), Sox4 (HPA029901, rabbit polyclonal, Sigma Aldrich, 1:30), CD3 (Clone PS1, mouse monoclonal, Leica Microsystems, 1:200), CD4 (Clone 1F6, mouse monoclonal, Leica Microsystems, 1:100), CXCR5 (Clone 51505, mouse monoclonal, R & D Systems, 1:1000), and PD-1 (Clone NAT105, mouse monoclonal, Abcam, 1:50) and detected with TSA Plus Fluorescence Kit (PerkinElmer, Inc.).

    Article Title: HCV Induces Oxidative and ER Stress, and Sensitizes Infected Cells to Apoptosis in SCID/Alb-uPA Mice
    Article Snippet: Paragraph title: Immunohistochemistry, immunofluorescence and confocal microscopy ... The peroxidase was developed using the TSA Plus fluorescence system with tyramide-tetramethyl red (Perkin Elmer).

    Article Title: Nfat/calcineurin signaling promotes oligodendrocyte differentiation and myelination by transcription factor network tuning
    Article Snippet: Paragraph title: Immunohistochemistry and in situ hybridization ... For anti-Nkx2.2 antibodies, signal intensity was enhanced by using the TSA-Plus Fluorescence system (PerkinElmer).

    RNA In Situ Hybridization:

    Article Title: A Pheromone Receptor Mediates 11-cis-Vaccenyl Acetate-Induced Responses in Drosophila
    Article Snippet: Paragraph title: RNA in situ hybridization. ... Signals were amplified with the TSA-Plus Cyanine 5 system (PerkinElmer, Wellesley, MA) for Or83b or the TSA-Plus Fluorescence system (PerkinElmer) for Or67d ( ).

    Cell Culture:

    Article Title: Dendritically targeted Bdnf mRNA is essential for energy balance and response to leptin
    Article Snippet: .. In situ hybridization Fluorescent in situ hybridization of cultured neurons was performed using digoxigenin-labeled riboprobes and the TSA Plus Fluorescein System (PerkinElmer, Waltham, MA) as previously described . .. Radioactive in situ hybridization of brain sections was performed using 35 S-labeled riboprobes as previously described .

    Article Title: Nfat/calcineurin signaling promotes oligodendrocyte differentiation and myelination by transcription factor network tuning
    Article Snippet: Immunohistochemistry and in situ hybridization were performed on cultured slices and 10 µm cryotome sections of electroporated chicken neural tube or mouse spinal cord (forelimb level) after fixation in 4% paraformaldehyde, transfer to 30% sucrose and freezing in Tissue Freezing Medium (Leica) as described . .. For anti-Nkx2.2 antibodies, signal intensity was enhanced by using the TSA-Plus Fluorescence system (PerkinElmer).

    Imaging:

    Article Title: Inhibition of ErbB kinase signalling promotes resolution of neutrophilic inflammation
    Article Snippet: TSA signal amplification of GFP-labelled neutrophils (driven by endogenous peroxidase activity) was carried out using TSA Plus Fluorescein System (Perkin Elmer). .. Larvae were then mounted in low-melting point agarose and images acquired and analysed using UltraVIEWVoX spinning disc confocal laser imaging system with Volocity 6.3 software (Perkin Elmer).

    Article Title: Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments
    Article Snippet: Triple immunofluorescence staining was performed with CXCL13 antibody (BCA1, rabbit polyclonal, Thermo Fisher, 1:100), Sox4 (HPA029901, rabbit polyclonal, Sigma Aldrich, 1:30), CD3 (Clone PS1, mouse monoclonal, Leica Microsystems, 1:200), CD4 (Clone 1F6, mouse monoclonal, Leica Microsystems, 1:100), CXCR5 (Clone 51505, mouse monoclonal, R & D Systems, 1:1000), and PD-1 (Clone NAT105, mouse monoclonal, Abcam, 1:50) and detected with TSA Plus Fluorescence Kit (PerkinElmer, Inc.). .. Fluorescence imaging analysis was performed using the FSX100 Fluorescence Microscope (Olympus).

    Inverted Microscopy:

    Article Title: Nfat/calcineurin signaling promotes oligodendrocyte differentiation and myelination by transcription factor network tuning
    Article Snippet: For anti-Nkx2.2 antibodies, signal intensity was enhanced by using the TSA-Plus Fluorescence system (PerkinElmer). .. Immunohistochemical stainings were documented with a Leica DMI6000 B inverted microscope (Leica) equipped with a DFC 360FX camera (Leica).

    Serial Time-encoded Amplified Microscopy:

    Article Title: The long noncoding RNA Vax2os1 controls the cell cycle progression of photoreceptor progenitors in the mouse retina
    Article Snippet: The eyes were orientated and flash-frozen in O.C.T (Kaltek) using liquid nitrogen steam. .. The ABC kit (Vector Laboratories) and TSA-plus fluorescein (Perkin Elmer) were used according to the manufacturer's instructions to amplify the signal in Ki67 staining on eye sections.

    Immunofluorescence:

    Article Title: Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments
    Article Snippet: .. Triple immunofluorescence staining was performed with CXCL13 antibody (BCA1, rabbit polyclonal, Thermo Fisher, 1:100), Sox4 (HPA029901, rabbit polyclonal, Sigma Aldrich, 1:30), CD3 (Clone PS1, mouse monoclonal, Leica Microsystems, 1:200), CD4 (Clone 1F6, mouse monoclonal, Leica Microsystems, 1:100), CXCR5 (Clone 51505, mouse monoclonal, R & D Systems, 1:1000), and PD-1 (Clone NAT105, mouse monoclonal, Abcam, 1:50) and detected with TSA Plus Fluorescence Kit (PerkinElmer, Inc.). .. Fluorescence imaging analysis was performed using the FSX100 Fluorescence Microscope (Olympus).

    Article Title: HCV Induces Oxidative and ER Stress, and Sensitizes Infected Cells to Apoptosis in SCID/Alb-uPA Mice
    Article Snippet: Paragraph title: Immunohistochemistry, immunofluorescence and confocal microscopy ... The peroxidase was developed using the TSA Plus fluorescence system with tyramide-tetramethyl red (Perkin Elmer).

    Article Title: Defining BMP functions in the hair follicle by conditional ablation of BMP receptor IA
    Article Snippet: Paragraph title: X-gal staining and immunofluorescence ... For phospho-Smad1, we used the TSA™ Plus Fluorescing System from Perkin Elmer Life Sciences.

    Article Title: The long noncoding RNA Vax2os1 controls the cell cycle progression of photoreceptor progenitors in the mouse retina
    Article Snippet: Paragraph title: Immunofluorescence and FACS ... The ABC kit (Vector Laboratories) and TSA-plus fluorescein (Perkin Elmer) were used according to the manufacturer's instructions to amplify the signal in Ki67 staining on eye sections.

    In Vivo:

    Article Title: The long noncoding RNA Vax2os1 controls the cell cycle progression of photoreceptor progenitors in the mouse retina
    Article Snippet: The primary antibodies used are a monoclonal anti-BrdU (mouse, clone BU-33; Sigma-Aldrich) 1:300 and anti-phospho-Histone-H3 (Ser10, rabbit; Millipore) 1:200 for both the in vitro and in vivo studies; anti-Ki67 (mouse, clone B56; BD Pharmingen) 1:100 for the in vitro studies and monoclonal anti-Ki67 (rat, clone TEC-3; Dako) for the in vivo studies; monoclonal anti-Neuronal class III β-tubulin (mouse, clone Tuj-1; Covance); anti-Rhodopsin (mouse, clone RET-P1; Sigma-Aldrich) 1:1000; monoclonal anti-acetylated-α-tubulin (mouse, clone 6-11B-1; Sigma-Aldrich) 1:500, anti-Opsin, Blue (sw) and Green/Red (mw) (rabbit; Millipore) 1:200; anti-Chx10 (goat, clone C-17; Santa Cruz Biotechnology) 1:100. .. The ABC kit (Vector Laboratories) and TSA-plus fluorescein (Perkin Elmer) were used according to the manufacturer's instructions to amplify the signal in Ki67 staining on eye sections.

    Fluorescence:

    Article Title: Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments
    Article Snippet: .. Triple immunofluorescence staining was performed with CXCL13 antibody (BCA1, rabbit polyclonal, Thermo Fisher, 1:100), Sox4 (HPA029901, rabbit polyclonal, Sigma Aldrich, 1:30), CD3 (Clone PS1, mouse monoclonal, Leica Microsystems, 1:200), CD4 (Clone 1F6, mouse monoclonal, Leica Microsystems, 1:100), CXCR5 (Clone 51505, mouse monoclonal, R & D Systems, 1:1000), and PD-1 (Clone NAT105, mouse monoclonal, Abcam, 1:50) and detected with TSA Plus Fluorescence Kit (PerkinElmer, Inc.). .. Fluorescence imaging analysis was performed using the FSX100 Fluorescence Microscope (Olympus).

    Article Title: HCV Induces Oxidative and ER Stress, and Sensitizes Infected Cells to Apoptosis in SCID/Alb-uPA Mice
    Article Snippet: .. The peroxidase was developed using the TSA Plus fluorescence system with tyramide-tetramethyl red (Perkin Elmer). .. Mounting media (Vectastain-Vector laboratories) contained 1 µg/ml 4,6-diamidino-2-phenylindole (DAPI).

    Article Title: The Nutrient-Responsive Hormone CCHamide-2 Controls Growth by Regulating Insulin-like Peptides in the Brain of Drosophila melanogaster
    Article Snippet: Digoxigenin-labeled RNA probes were detected using the TSA Plus Fluorescein Kit (PerkinElmer). .. Images were analyzed by LSM700 confocal microscopy (Zeiss) or DM5000B fluorescence microscopy with a DFC500 CCD camera (Leica).

    Article Title: Organ-specific requirements for Hdac1 in liver and pancreas formation
    Article Snippet: .. Fluorescent in situ hybridisations were carried out using the TSA Plus Fluorescence palette system (Perkin-Elmer). .. Anti-digoxygenin-HRP (Roche) and anti-fluorescein-HRP (Perkin-Elmer) were used at 1:1000; tyramide-labelled substrate was used at 1:50.

    Article Title: A Pheromone Receptor Mediates 11-cis-Vaccenyl Acetate-Induced Responses in Drosophila
    Article Snippet: .. Signals were amplified with the TSA-Plus Cyanine 5 system (PerkinElmer, Wellesley, MA) for Or83b or the TSA-Plus Fluorescence system (PerkinElmer) for Or67d ( ). .. A cDNA encoding Or67d was obtained by reverse transcription, PCR amplification of RNA isolated from antenna of Drosophila w1118 , a strain known to be responsive to VA pheromone (our unpublished observation) (see Figs. 1, 4).

    Article Title: Nfat/calcineurin signaling promotes oligodendrocyte differentiation and myelination by transcription factor network tuning
    Article Snippet: .. For anti-Nkx2.2 antibodies, signal intensity was enhanced by using the TSA-Plus Fluorescence system (PerkinElmer). .. Secondary antibodies were coupled to Cy3 (Dianova, 1:200 dilution) or Alexa488 (Molecular Probes, 1:500 dilution) fluorescent dyes.

    Article Title: Rhythmic Trafficking of TRPV2 in the Suprachiasmatic Nucleus is Regulated by Prokineticin 2 Signaling
    Article Snippet: .. DIG labeled cells were revealed with the TSA Plus Fluorescence Kit from Perkin Elmer as described in the instruction manual. ..

    Avidin-Biotin Assay:

    Article Title: HCV Induces Oxidative and ER Stress, and Sensitizes Infected Cells to Apoptosis in SCID/Alb-uPA Mice
    Article Snippet: The peroxidase was developed using the TSA Plus fluorescence system with tyramide-tetramethyl red (Perkin Elmer). .. For BiP/GRP78, the staining procedure was essentially the same, except slides were blocked with normal donkey serum and avidin/biotin block (Vector laboratories), the primary antibodies were goat anti-GRP78/Bip with the mouse anti-HCV, and the secondary antibodies were donkey anti-goat alexa 488 (Molecular probes), and donkey anti-mouse biotin, followed by avidin-HRP (Vector laboratories).

    Labeling:

    Article Title: Increased B Cell ADAM10 in Allergic Patients and Th2 Prone Mice
    Article Snippet: .. TNF labeling was enhanced using Fluorescein-TSA (TSA Plus Fluorescein System, Perkin Elmer, NEL741001KT) [ ]. ..

    Article Title: Rhythmic Trafficking of TRPV2 in the Suprachiasmatic Nucleus is Regulated by Prokineticin 2 Signaling
    Article Snippet: .. DIG labeled cells were revealed with the TSA Plus Fluorescence Kit from Perkin Elmer as described in the instruction manual. ..

    Immunostaining:

    Article Title: The Nutrient-Responsive Hormone CCHamide-2 Controls Growth by Regulating Insulin-like Peptides in the Brain of Drosophila melanogaster
    Article Snippet: Paragraph title: Immunostaining and FISH ... Digoxigenin-labeled RNA probes were detected using the TSA Plus Fluorescein Kit (PerkinElmer).

    Confocal Microscopy:

    Article Title: Increased B Cell ADAM10 in Allergic Patients and Th2 Prone Mice
    Article Snippet: Paragraph title: Immunohistochemistry and confocal microscopy ... TNF labeling was enhanced using Fluorescein-TSA (TSA Plus Fluorescein System, Perkin Elmer, NEL741001KT) [ ].

    Article Title: HCV Induces Oxidative and ER Stress, and Sensitizes Infected Cells to Apoptosis in SCID/Alb-uPA Mice
    Article Snippet: Paragraph title: Immunohistochemistry, immunofluorescence and confocal microscopy ... The peroxidase was developed using the TSA Plus fluorescence system with tyramide-tetramethyl red (Perkin Elmer).

    Article Title: The Nutrient-Responsive Hormone CCHamide-2 Controls Growth by Regulating Insulin-like Peptides in the Brain of Drosophila melanogaster
    Article Snippet: Digoxigenin-labeled RNA probes were detected using the TSA Plus Fluorescein Kit (PerkinElmer). .. Images were analyzed by LSM700 confocal microscopy (Zeiss) or DM5000B fluorescence microscopy with a DFC500 CCD camera (Leica).

    Staining:

    Article Title: Inhibition of ErbB kinase signalling promotes resolution of neutrophilic inflammation
    Article Snippet: TSA signal amplification of GFP-labelled neutrophils (driven by endogenous peroxidase activity) was carried out using TSA Plus Fluorescein System (Perkin Elmer). .. Larvae were post-fixed in 4% paraformaldehyde, before subsequent TUNEL staining for apoptosis using ApopTag Red In Situ Apoptosis Detection Kit (Millipore).

    Article Title: Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments
    Article Snippet: .. Triple immunofluorescence staining was performed with CXCL13 antibody (BCA1, rabbit polyclonal, Thermo Fisher, 1:100), Sox4 (HPA029901, rabbit polyclonal, Sigma Aldrich, 1:30), CD3 (Clone PS1, mouse monoclonal, Leica Microsystems, 1:200), CD4 (Clone 1F6, mouse monoclonal, Leica Microsystems, 1:100), CXCR5 (Clone 51505, mouse monoclonal, R & D Systems, 1:1000), and PD-1 (Clone NAT105, mouse monoclonal, Abcam, 1:50) and detected with TSA Plus Fluorescence Kit (PerkinElmer, Inc.). .. Fluorescence imaging analysis was performed using the FSX100 Fluorescence Microscope (Olympus).

    Article Title: HCV Induces Oxidative and ER Stress, and Sensitizes Infected Cells to Apoptosis in SCID/Alb-uPA Mice
    Article Snippet: Inactive Bax was scored in the same manner as BiP, and the scale for activated Bax was 0 is no staining, 1(1–5%), 2 (5–10%), and 3 (10–15%). .. The peroxidase was developed using the TSA Plus fluorescence system with tyramide-tetramethyl red (Perkin Elmer).

    Article Title: The Nutrient-Responsive Hormone CCHamide-2 Controls Growth by Regulating Insulin-like Peptides in the Brain of Drosophila melanogaster
    Article Snippet: Immunostaining and FISH Antibody staining was conducted as described previously [ ], except that the gut samples were fixed in 4% formaldehyde in PBS for 4 hours. .. Digoxigenin-labeled RNA probes were detected using the TSA Plus Fluorescein Kit (PerkinElmer).

    Article Title: Defining BMP functions in the hair follicle by conditional ablation of BMP receptor IA
    Article Snippet: Paragraph title: X-gal staining and immunofluorescence ... For phospho-Smad1, we used the TSA™ Plus Fluorescing System from Perkin Elmer Life Sciences.

    Article Title: The long noncoding RNA Vax2os1 controls the cell cycle progression of photoreceptor progenitors in the mouse retina
    Article Snippet: .. The ABC kit (Vector Laboratories) and TSA-plus fluorescein (Perkin Elmer) were used according to the manufacturer's instructions to amplify the signal in Ki67 staining on eye sections. .. Slides were incubated with the Alexa Fluor secondary antibodies (Invitrogen) 1:1000.

    In Situ Hybridization:

    Article Title: Dendritically targeted Bdnf mRNA is essential for energy balance and response to leptin
    Article Snippet: .. In situ hybridization Fluorescent in situ hybridization of cultured neurons was performed using digoxigenin-labeled riboprobes and the TSA Plus Fluorescein System (PerkinElmer, Waltham, MA) as previously described . .. Radioactive in situ hybridization of brain sections was performed using 35 S-labeled riboprobes as previously described .

    Article Title: Nfat/calcineurin signaling promotes oligodendrocyte differentiation and myelination by transcription factor network tuning
    Article Snippet: Paragraph title: Immunohistochemistry and in situ hybridization ... For anti-Nkx2.2 antibodies, signal intensity was enhanced by using the TSA-Plus Fluorescence system (PerkinElmer).

    Article Title: Rhythmic Trafficking of TRPV2 in the Suprachiasmatic Nucleus is Regulated by Prokineticin 2 Signaling
    Article Snippet: Paragraph title: In situ hybridization and colocalization ... DIG labeled cells were revealed with the TSA Plus Fluorescence Kit from Perkin Elmer as described in the instruction manual.

    Plasmid Preparation:

    Article Title: HCV Induces Oxidative and ER Stress, and Sensitizes Infected Cells to Apoptosis in SCID/Alb-uPA Mice
    Article Snippet: The peroxidase was developed using the TSA Plus fluorescence system with tyramide-tetramethyl red (Perkin Elmer). .. For BiP/GRP78, the staining procedure was essentially the same, except slides were blocked with normal donkey serum and avidin/biotin block (Vector laboratories), the primary antibodies were goat anti-GRP78/Bip with the mouse anti-HCV, and the secondary antibodies were donkey anti-goat alexa 488 (Molecular probes), and donkey anti-mouse biotin, followed by avidin-HRP (Vector laboratories).

    Article Title: The long noncoding RNA Vax2os1 controls the cell cycle progression of photoreceptor progenitors in the mouse retina
    Article Snippet: The ABC kit (Vector Laboratories) and TSA-plus fluorescein (Perkin Elmer) were used according to the manufacturer's instructions to amplify the signal in Ki67 staining on eye sections. .. The ABC kit (Vector Laboratories) and TSA-plus fluorescein (Perkin Elmer) were used according to the manufacturer's instructions to amplify the signal in Ki67 staining on eye sections.

    Article Title: A Pheromone Receptor Mediates 11-cis-Vaccenyl Acetate-Induced Responses in Drosophila
    Article Snippet: Antisense and sense riboprobes were transcribed from Or67d and Or83b cDNAs subcloned into PCR2.1-cloning vector (Invitrogen) using T7 RNA polymerase (Ambion). .. Signals were amplified with the TSA-Plus Cyanine 5 system (PerkinElmer, Wellesley, MA) for Or83b or the TSA-Plus Fluorescence system (PerkinElmer) for Or67d ( ).

    Software:

    Article Title: Inhibition of ErbB kinase signalling promotes resolution of neutrophilic inflammation
    Article Snippet: TSA signal amplification of GFP-labelled neutrophils (driven by endogenous peroxidase activity) was carried out using TSA Plus Fluorescein System (Perkin Elmer). .. Larvae were then mounted in low-melting point agarose and images acquired and analysed using UltraVIEWVoX spinning disc confocal laser imaging system with Volocity 6.3 software (Perkin Elmer).

    Microscopy:

    Article Title: Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments
    Article Snippet: Triple immunofluorescence staining was performed with CXCL13 antibody (BCA1, rabbit polyclonal, Thermo Fisher, 1:100), Sox4 (HPA029901, rabbit polyclonal, Sigma Aldrich, 1:30), CD3 (Clone PS1, mouse monoclonal, Leica Microsystems, 1:200), CD4 (Clone 1F6, mouse monoclonal, Leica Microsystems, 1:100), CXCR5 (Clone 51505, mouse monoclonal, R & D Systems, 1:1000), and PD-1 (Clone NAT105, mouse monoclonal, Abcam, 1:50) and detected with TSA Plus Fluorescence Kit (PerkinElmer, Inc.). .. Fluorescence imaging analysis was performed using the FSX100 Fluorescence Microscope (Olympus).

    Article Title: The Nutrient-Responsive Hormone CCHamide-2 Controls Growth by Regulating Insulin-like Peptides in the Brain of Drosophila melanogaster
    Article Snippet: Digoxigenin-labeled RNA probes were detected using the TSA Plus Fluorescein Kit (PerkinElmer). .. Images were analyzed by LSM700 confocal microscopy (Zeiss) or DM5000B fluorescence microscopy with a DFC500 CCD camera (Leica).

    In Vitro:

    Article Title: The long noncoding RNA Vax2os1 controls the cell cycle progression of photoreceptor progenitors in the mouse retina
    Article Snippet: The primary antibodies used are a monoclonal anti-BrdU (mouse, clone BU-33; Sigma-Aldrich) 1:300 and anti-phospho-Histone-H3 (Ser10, rabbit; Millipore) 1:200 for both the in vitro and in vivo studies; anti-Ki67 (mouse, clone B56; BD Pharmingen) 1:100 for the in vitro studies and monoclonal anti-Ki67 (rat, clone TEC-3; Dako) for the in vivo studies; monoclonal anti-Neuronal class III β-tubulin (mouse, clone Tuj-1; Covance); anti-Rhodopsin (mouse, clone RET-P1; Sigma-Aldrich) 1:1000; monoclonal anti-acetylated-α-tubulin (mouse, clone 6-11B-1; Sigma-Aldrich) 1:500, anti-Opsin, Blue (sw) and Green/Red (mw) (rabbit; Millipore) 1:200; anti-Chx10 (goat, clone C-17; Santa Cruz Biotechnology) 1:100. .. The ABC kit (Vector Laboratories) and TSA-plus fluorescein (Perkin Elmer) were used according to the manufacturer's instructions to amplify the signal in Ki67 staining on eye sections.

    Next-Generation Sequencing:

    Article Title: Defining BMP functions in the hair follicle by conditional ablation of BMP receptor IA
    Article Snippet: In other cases, the following block/diluent was used 2.5% NDS, 2.5% NGS, 1% BSA, 2% gelatin, and 1% Triton X-100 in PBS. .. For phospho-Smad1, we used the TSA™ Plus Fluorescing System from Perkin Elmer Life Sciences.

    Knock-Out:

    Article Title: Nfat/calcineurin signaling promotes oligodendrocyte differentiation and myelination by transcription factor network tuning
    Article Snippet: For immunohistochemistry, the following primary antibodies were applied: rat anti-MBP monoclonal (Abcam, #ab7349, Lot# GR188102-12, 1:500 dilution and Bio-Rad, #MCA409S, Lot #210610, 1:500 dilution), mouse anti-Neurofilament L monoclonal (Dako, clone 2F11, Lot# 20014701, 1:500 dilution), mouse anti-Nkx2.2 monoclonal (Developmental Studies Hybridoma Bank, University of Iowa, clone 74.5A5, 1:5000 dilution), guinea pig anti-Sox10 antiserum (home made, validated on wildtpye and knockout mouse tissue, 1:1000 dilution) , rabbit anti-Myrf antiserum (home made, validated on wildtpye and knockout mouse tissue, 1:1000 dilution) , rabbit anti-Olig2 antiserum (Millipore, #AB9610, Lot# 2060464, 1:1000 dilution), rabbit anti-Pdgfra antiserum (Santa Cruz, #sc-338, Lot# E-1210, 1:300 dilution), rabbit anti-cleaved caspase 3 antiserum (Cell Signaling Technology, #9661, Lot# 0043, 1:200 dilution), rabbit anti-Ki67 antiserum (Thermo Fisher Scientific, #RM-9106, Lot# 9106S906D, 1:500 dilution), rabbit anti-GFP antiserum (Molecular Probes, # , Lot# 1293114, 1:2000 dilution), goat anti-tdTomato antiserum (Sicgen, #AB8181-200, Lot# 8181240714, 1:1000 dilution). .. For anti-Nkx2.2 antibodies, signal intensity was enhanced by using the TSA-Plus Fluorescence system (PerkinElmer).

    In Situ:

    Article Title: Inhibition of ErbB kinase signalling promotes resolution of neutrophilic inflammation
    Article Snippet: TSA signal amplification of GFP-labelled neutrophils (driven by endogenous peroxidase activity) was carried out using TSA Plus Fluorescein System (Perkin Elmer). .. Larvae were post-fixed in 4% paraformaldehyde, before subsequent TUNEL staining for apoptosis using ApopTag Red In Situ Apoptosis Detection Kit (Millipore).

    Article Title: HCV Induces Oxidative and ER Stress, and Sensitizes Infected Cells to Apoptosis in SCID/Alb-uPA Mice
    Article Snippet: The peroxidase was developed using the TSA Plus fluorescence system with tyramide-tetramethyl red (Perkin Elmer). .. For co-localization of HCV antigens and TUNEL reactivity, the In Situ Cell Death Detection kit (fluorescein) (Roche) was used, according to the manufacturer's specifications.

    Article Title: Organ-specific requirements for Hdac1 in liver and pancreas formation
    Article Snippet: .. Fluorescent in situ hybridisations were carried out using the TSA Plus Fluorescence palette system (Perkin-Elmer). .. Anti-digoxygenin-HRP (Roche) and anti-fluorescein-HRP (Perkin-Elmer) were used at 1:1000; tyramide-labelled substrate was used at 1:50.

    FACS:

    Article Title: The long noncoding RNA Vax2os1 controls the cell cycle progression of photoreceptor progenitors in the mouse retina
    Article Snippet: Paragraph title: Immunofluorescence and FACS ... The ABC kit (Vector Laboratories) and TSA-plus fluorescein (Perkin Elmer) were used according to the manufacturer's instructions to amplify the signal in Ki67 staining on eye sections.

    Fluorescence In Situ Hybridization:

    Article Title: The Nutrient-Responsive Hormone CCHamide-2 Controls Growth by Regulating Insulin-like Peptides in the Brain of Drosophila melanogaster
    Article Snippet: Paragraph title: Immunostaining and FISH ... Digoxigenin-labeled RNA probes were detected using the TSA Plus Fluorescein Kit (PerkinElmer).

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    PerkinElmer tsa plus fluorscence palette system
    Tsa Plus Fluorscence Palette System, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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