Structured Review

Nissui Pharmaceutical trypticase soy broth
Trypticase Soy Broth, supplied by Nissui Pharmaceutical, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trypticase soy broth/product/Nissui Pharmaceutical
Average 93 stars, based on 2 article reviews
Price from $9.99 to $1999.99
trypticase soy broth - by Bioz Stars, 2020-04
93/100 stars

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Centrifugation:

Article Title: Molecular characterization of cytolethal distending toxin gene-positive Escherichia coli from healthy cattle and swine in Nara, Japan
Article Snippet: Detection of cdtB gene by PCR Aliquot of stool specimens and rectal swabs were inoculated into 3 mL of tryptic soy broth (Nissui Pharmaceutical Co., Tokyo, Japan) for enrichment and incubated overnight at 37°C with shaking. .. Fifty microliter of the culture was added into 450 μL of TE buffer (10 mM Tris–HCl, 1 mM EDTA [pH 8.0]) and boiled for 10 min. After centrifugation at 12,000 × g for 3 min, the supernatant was used as a template for PCR in a thermal cycler (GeneAmp PCR System 9700; Life Technologies, Carlsbad, CA, USA).

Article Title: Porphyromonas gingivalis Outer Membrane Vesicles Enter Human Epithelial Cells via an Endocytic Pathway and Are Sorted to Lysosomal Compartments
Article Snippet: The organisms were anaerobically maintained in blood agar plates, and grown in trypticase soy broth (Nissui, Tokyo, Japan) supplemented with hemin (5 μg/ml; Wako Pure Chemical Industries) and menadione (1 μg/ml; Sigma-Aldrich), as described previously ( ). .. Briefly, P. gingivalis cells were removed from the culture by centrifugation at 10,000 × g for 30 min at 4°C.

Amplification:

Article Title: Seasonal Change in Bacterial Flora and Biomass in Mountain Snow from the Tateyama Mountains, Japan, Analyzed by 16S rRNA Gene Sequencing and Real-Time PCR
Article Snippet: To obtain the densities of specific bacteria in snow samples, we established standard amplification curves with known numbers of specific bacteria and their threshold cycles. .. C. psychrophilum was cultured in Trypticase soy broth (Nissui, Tokyo, Japan) for 24 h at 4°C with shaking (80 rpm), and J. lividum and V. paradoxus were cultured in nutrient broth A medium (5.0 g of peptone/liter, 3.0 g of beef extract/liter, and 3.0 g of NaCl/liter) for 12 h at 25°C with shaking (80 rpm).

Filtration:

Article Title: Porphyromonas gingivalis Outer Membrane Vesicles Enter Human Epithelial Cells via an Endocytic Pathway and Are Sorted to Lysosomal Compartments
Article Snippet: The organisms were anaerobically maintained in blood agar plates, and grown in trypticase soy broth (Nissui, Tokyo, Japan) supplemented with hemin (5 μg/ml; Wako Pure Chemical Industries) and menadione (1 μg/ml; Sigma-Aldrich), as described previously ( ). .. Following filtration of the supernatant with Millipore filters (pore size, 0.22 μm), MVs were collected as pellet by ultracentrifugation at 100,000 × g for 3 h at 4°C.

Mass Spectrometry:

Article Title: Extended-spectrum β-lactamase-producing Escherichia coliisolated from healthy Thoroughbred racehorses in Japan
Article Snippet: Samples were subcultured in trypticase soy broth (Nissui Pharmaceutical Co., Ltd., Tokyo, Japan) and incubated for 24 hr at 37°C. .. Selected isolates were confirmed to the species level using MALDI-TOF MS (Bruker, Billerica, MA, U.S.A.).

TA Cloning:

Article Title: Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces
Article Snippet: TA cloning of polymerase chain reaction (PCR)‐generated DNA fragments was done with the help of pMD19 (Takara Shuzo). pUWLFLP carrying an Flp recombinase gene ( ) was used for the construction of a marker‐less mutant. .. Streptomyces strains were cultured at 28°C in Bennett's/sugar medium [composition: 1 g l−1 yeast extract (Difco Laboratories, Detroit, Michigan), 1 g l−1 meat extract (Kyokuto, Tokyo, Japan), 2 g l−1 NZ amine (Wako Pure Chemical Industries, Osaka, Japan) and 10 g l−1 of the appropriate sugar (pH 7.2)], R2YE medium , YMP/sugar medium , tryptic soy broth (TSB; Nissui, Tokyo, Japan) and minimal medium [composition: 0.5 g l−1 K2 HPO4 , 0.2 g l−1 MgSO4 7H2 O, 0.01 g l−1 FeSO4 7H2 O, 1.0 g l−1 (NH4 )2 SO4 and 10 g l−1 of the appropriate sugar (pH 7.2)].

Real-time Polymerase Chain Reaction:

Article Title: Seasonal Change in Bacterial Flora and Biomass in Mountain Snow from the Tateyama Mountains, Japan, Analyzed by 16S rRNA Gene Sequencing and Real-Time PCR
Article Snippet: Paragraph title: Real-time PCR for quantification of specific bacteria. ... C. psychrophilum was cultured in Trypticase soy broth (Nissui, Tokyo, Japan) for 24 h at 4°C with shaking (80 rpm), and J. lividum and V. paradoxus were cultured in nutrient broth A medium (5.0 g of peptone/liter, 3.0 g of beef extract/liter, and 3.0 g of NaCl/liter) for 12 h at 25°C with shaking (80 rpm).

Incubation:

Article Title: Extended-spectrum β-lactamase-producing Escherichia coliisolated from healthy Thoroughbred racehorses in Japan
Article Snippet: .. Samples were subcultured in trypticase soy broth (Nissui Pharmaceutical Co., Ltd., Tokyo, Japan) and incubated for 24 hr at 37°C. .. Each sample was cultivated onto MacConkey agar (Nissui Pharmaceutical Co., Ltd.) supplemented with 1µ g/ml cefotaxime (CTX) (Duchefa Biochemie B.V. Haarlem, North Holland, the Netherlands) and was incubated for 24 hr at 37°C for colony isolation.

Article Title: Molecular characterization of cytolethal distending toxin gene-positive Escherichia coli from healthy cattle and swine in Nara, Japan
Article Snippet: .. Detection of cdtB gene by PCR Aliquot of stool specimens and rectal swabs were inoculated into 3 mL of tryptic soy broth (Nissui Pharmaceutical Co., Tokyo, Japan) for enrichment and incubated overnight at 37°C with shaking. .. Fifty microliter of the culture was added into 450 μL of TE buffer (10 mM Tris–HCl, 1 mM EDTA [pH 8.0]) and boiled for 10 min. After centrifugation at 12,000 × g for 3 min, the supernatant was used as a template for PCR in a thermal cycler (GeneAmp PCR System 9700; Life Technologies, Carlsbad, CA, USA).

Article Title: In Vitro and In Vivo Activities of Levofloxacin against Biofilm-Producing Pseudomonas aeruginosa
Article Snippet: .. Overnight cultures in tryptic soy broth were diluted with fresh heart infusion broth (Nissui Seiyaku, Tokyo, Japan) to about 105 CFU/ml and were then incubated for a further 2 to 3 h with shaking at 37°C. ..

Article Title: Escherichia coli Producing CTX-M-2 ?-Lactamase in Cattle, Japan
Article Snippet: .. The remaining Trypticase soy broth with bacteria was further incubated overnight. ..

Article Title: Association of Veterinary Third-Generation Cephalosporin Use with the Risk of Emergence of Extended-Spectrum-Cephalosporin Resistance in Escherichia coli from Dairy Cattle in Japan
Article Snippet: Donors and recipients were grown in tryptic soy broth (TSB, Nissui) to the logarithmic phase; they were then mixed in a total volume of 2 mL at a 1∶9 (v/v) ratio, and 2 mL fresh TSB was added. .. The mating cultures were incubated overnight at 37°C.

Activity Assay:

Article Title: In Vitro and In Vivo Activities of Levofloxacin against Biofilm-Producing Pseudomonas aeruginosa
Article Snippet: Paragraph title: Bactericidal activity of antibacterials against nongrowing bacteria. ... Overnight cultures in tryptic soy broth were diluted with fresh heart infusion broth (Nissui Seiyaku, Tokyo, Japan) to about 105 CFU/ml and were then incubated for a further 2 to 3 h with shaking at 37°C.

Cell Culture:

Article Title: Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces
Article Snippet: .. Streptomyces strains were cultured at 28°C in Bennett's/sugar medium [composition: 1 g l−1 yeast extract (Difco Laboratories, Detroit, Michigan), 1 g l−1 meat extract (Kyokuto, Tokyo, Japan), 2 g l−1 NZ amine (Wako Pure Chemical Industries, Osaka, Japan) and 10 g l−1 of the appropriate sugar (pH 7.2)], R2YE medium , YMP/sugar medium , tryptic soy broth (TSB; Nissui, Tokyo, Japan) and minimal medium [composition: 0.5 g l−1 K2 HPO4 , 0.2 g l−1 MgSO4 7H2 O, 0.01 g l−1 FeSO4 7H2 O, 1.0 g l−1 (NH4 )2 SO4 and 10 g l−1 of the appropriate sugar (pH 7.2)]. ..

Article Title: Seasonal Change in Bacterial Flora and Biomass in Mountain Snow from the Tateyama Mountains, Japan, Analyzed by 16S rRNA Gene Sequencing and Real-Time PCR
Article Snippet: .. C. psychrophilum was cultured in Trypticase soy broth (Nissui, Tokyo, Japan) for 24 h at 4°C with shaking (80 rpm), and J. lividum and V. paradoxus were cultured in nutrient broth A medium (5.0 g of peptone/liter, 3.0 g of beef extract/liter, and 3.0 g of NaCl/liter) for 12 h at 25°C with shaking (80 rpm). ..

Expressing:

Article Title: Immunostimulating Properties of Intragastrically Administered Acetobacter-Derived Soluble Branched (1,4)-?-d-Glucans Decrease Murine Susceptibility to Listeria monocytogenes
Article Snippet: In some experiments, recombinant L. monocytogenes expressing OVA (r LM -OVA), kindly provided by Subash Sad (Institute for Biological Sciences, Ottawa, Ontario, Canada) was used ( , ). .. Fresh isolates were obtained from infected spleens, grown in tryptic soy broth (Nissui Pharmaceutical Co., Ltd., Tokyo, Japan), washed repeatedly, resuspended in phosphate-buffered saline (PBS), and stored at −70°C in small aliquots.

Derivative Assay:

Article Title: Porphyromonas gingivalis Outer Membrane Vesicles Enter Human Epithelial Cells via an Endocytic Pathway and Are Sorted to Lysosomal Compartments
Article Snippet: P. gingivalis strain ATCC 33277 and its derived fimbria-null mutant strain KDP150 (Δ fimA ) , which was kindly provided by K. Nakayama (Nagasaki University, Japan), were used in this study. .. The organisms were anaerobically maintained in blood agar plates, and grown in trypticase soy broth (Nissui, Tokyo, Japan) supplemented with hemin (5 μg/ml; Wako Pure Chemical Industries) and menadione (1 μg/ml; Sigma-Aldrich), as described previously ( ).

Infection:

Article Title: Immunostimulating Properties of Intragastrically Administered Acetobacter-Derived Soluble Branched (1,4)-?-d-Glucans Decrease Murine Susceptibility to Listeria monocytogenes
Article Snippet: .. Fresh isolates were obtained from infected spleens, grown in tryptic soy broth (Nissui Pharmaceutical Co., Ltd., Tokyo, Japan), washed repeatedly, resuspended in phosphate-buffered saline (PBS), and stored at −70°C in small aliquots. ..

Polymerase Chain Reaction:

Article Title: Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces
Article Snippet: TA cloning of polymerase chain reaction (PCR)‐generated DNA fragments was done with the help of pMD19 (Takara Shuzo). pUWLFLP carrying an Flp recombinase gene ( ) was used for the construction of a marker‐less mutant. .. Streptomyces strains were cultured at 28°C in Bennett's/sugar medium [composition: 1 g l−1 yeast extract (Difco Laboratories, Detroit, Michigan), 1 g l−1 meat extract (Kyokuto, Tokyo, Japan), 2 g l−1 NZ amine (Wako Pure Chemical Industries, Osaka, Japan) and 10 g l−1 of the appropriate sugar (pH 7.2)], R2YE medium , YMP/sugar medium , tryptic soy broth (TSB; Nissui, Tokyo, Japan) and minimal medium [composition: 0.5 g l−1 K2 HPO4 , 0.2 g l−1 MgSO4 7H2 O, 0.01 g l−1 FeSO4 7H2 O, 1.0 g l−1 (NH4 )2 SO4 and 10 g l−1 of the appropriate sugar (pH 7.2)].

Article Title: Molecular characterization of cytolethal distending toxin gene-positive Escherichia coli from healthy cattle and swine in Nara, Japan
Article Snippet: .. Detection of cdtB gene by PCR Aliquot of stool specimens and rectal swabs were inoculated into 3 mL of tryptic soy broth (Nissui Pharmaceutical Co., Tokyo, Japan) for enrichment and incubated overnight at 37°C with shaking. .. Fifty microliter of the culture was added into 450 μL of TE buffer (10 mM Tris–HCl, 1 mM EDTA [pH 8.0]) and boiled for 10 min. After centrifugation at 12,000 × g for 3 min, the supernatant was used as a template for PCR in a thermal cycler (GeneAmp PCR System 9700; Life Technologies, Carlsbad, CA, USA).

Recombinant:

Article Title: Immunostimulating Properties of Intragastrically Administered Acetobacter-Derived Soluble Branched (1,4)-?-d-Glucans Decrease Murine Susceptibility to Listeria monocytogenes
Article Snippet: In some experiments, recombinant L. monocytogenes expressing OVA (r LM -OVA), kindly provided by Subash Sad (Institute for Biological Sciences, Ottawa, Ontario, Canada) was used ( , ). .. Fresh isolates were obtained from infected spleens, grown in tryptic soy broth (Nissui Pharmaceutical Co., Ltd., Tokyo, Japan), washed repeatedly, resuspended in phosphate-buffered saline (PBS), and stored at −70°C in small aliquots.

DNA Extraction:

Article Title: Identification and Characterization of Two Novel Staphylococcal Enterotoxins, Types S and T ▿
Article Snippet: .. S. aureus strains were grown in two media: Trypticase soy broth (Nissui, Tokyo, Japan), kept at 37°C and maintained with aeration for total DNA isolation, and brain heart infusion broth (Difco Laboratories, Detroit, MI) supplemented with 1% yeast extract (Difco) at 37°C with aeration for RNA isolation. .. SE proteins were produced by S. aureus strains grown in brain heart infusion broth supplemented with 1% yeast extract at 37°C for 48 h with aeration ( ).

Article Title: Identification and Characterization of a Novel Staphylococcal Emetic Toxin
Article Snippet: .. S. aureus was grown in Trypticase soy broth (Nissui Pharmaceutical Co., Tokyo, Japan) at 37°C with aeration for total DNA isolation. .. Escherichia coli strains were grown in LB broth (Sigma-Aldrich, St. Louis, MO) containing 100 μg/ml of ampicillin (Wako Pure Chemicals, Osaka, Japan) for plasmid isolation.

Methylation:

Article Title: Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces
Article Snippet: The Escherichia coli DH5α strain (Takara Shuzo; Kyoto, Japan) was used as a host for conventional DNA manipulation, and the GM2163 strain, a methylation‐deficient strain, was used for generating disruption cosmids. pUC19 (Takara Shuzo) was used for general DNA manipulation. .. Streptomyces strains were cultured at 28°C in Bennett's/sugar medium [composition: 1 g l−1 yeast extract (Difco Laboratories, Detroit, Michigan), 1 g l−1 meat extract (Kyokuto, Tokyo, Japan), 2 g l−1 NZ amine (Wako Pure Chemical Industries, Osaka, Japan) and 10 g l−1 of the appropriate sugar (pH 7.2)], R2YE medium , YMP/sugar medium , tryptic soy broth (TSB; Nissui, Tokyo, Japan) and minimal medium [composition: 0.5 g l−1 K2 HPO4 , 0.2 g l−1 MgSO4 7H2 O, 0.01 g l−1 FeSO4 7H2 O, 1.0 g l−1 (NH4 )2 SO4 and 10 g l−1 of the appropriate sugar (pH 7.2)].

Mutagenesis:

Article Title: Entry of Porphyromonas gingivalis Outer Membrane Vesicles into Epithelial Cells Causes Cellular Functional Impairment ▿
Article Snippet: P. gingivalis strain ATCC 33277, as well as the ATCC 33277-derived mutant strains KDP129 (Δ kgp ), KDP133 (Δ rgpA Δ rgpB ), KDP136 (Δ kgp Δ rgpA Δ rgpB ) , and KDP150 (Δ fimA ) , kindly provided by K. Nakayama (Nagasaki University, Japan), were used in this study. .. The organisms were anaerobically maintained in blood agar plates and grown in Trypticase soy broth (Nissui, Tokyo, Japan) supplemented with hemin (5 μg/ml; Wako Pure Chemical Industries, Osaka, Japan) and menadione (1 μg/ml; Sigma-Aldrich, St. Louis, MO), as described previously ( ).

Article Title: Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces
Article Snippet: TA cloning of polymerase chain reaction (PCR)‐generated DNA fragments was done with the help of pMD19 (Takara Shuzo). pUWLFLP carrying an Flp recombinase gene ( ) was used for the construction of a marker‐less mutant. .. Streptomyces strains were cultured at 28°C in Bennett's/sugar medium [composition: 1 g l−1 yeast extract (Difco Laboratories, Detroit, Michigan), 1 g l−1 meat extract (Kyokuto, Tokyo, Japan), 2 g l−1 NZ amine (Wako Pure Chemical Industries, Osaka, Japan) and 10 g l−1 of the appropriate sugar (pH 7.2)], R2YE medium , YMP/sugar medium , tryptic soy broth (TSB; Nissui, Tokyo, Japan) and minimal medium [composition: 0.5 g l−1 K2 HPO4 , 0.2 g l−1 MgSO4 7H2 O, 0.01 g l−1 FeSO4 7H2 O, 1.0 g l−1 (NH4 )2 SO4 and 10 g l−1 of the appropriate sugar (pH 7.2)].

Article Title: Porphyromonas gingivalis Outer Membrane Vesicles Enter Human Epithelial Cells via an Endocytic Pathway and Are Sorted to Lysosomal Compartments
Article Snippet: P. gingivalis strain ATCC 33277 and its derived fimbria-null mutant strain KDP150 (Δ fimA ) , which was kindly provided by K. Nakayama (Nagasaki University, Japan), were used in this study. .. The organisms were anaerobically maintained in blood agar plates, and grown in trypticase soy broth (Nissui, Tokyo, Japan) supplemented with hemin (5 μg/ml; Wako Pure Chemical Industries) and menadione (1 μg/ml; Sigma-Aldrich), as described previously ( ).

Isolation:

Article Title: Extended-spectrum β-lactamase-producing Escherichia coliisolated from healthy Thoroughbred racehorses in Japan
Article Snippet: Paragraph title: Isolation of ESBL-producing E. coli ... Samples were subcultured in trypticase soy broth (Nissui Pharmaceutical Co., Ltd., Tokyo, Japan) and incubated for 24 hr at 37°C.

Article Title: Identification and Characterization of Two Novel Staphylococcal Enterotoxins, Types S and T ▿
Article Snippet: .. S. aureus strains were grown in two media: Trypticase soy broth (Nissui, Tokyo, Japan), kept at 37°C and maintained with aeration for total DNA isolation, and brain heart infusion broth (Difco Laboratories, Detroit, MI) supplemented with 1% yeast extract (Difco) at 37°C with aeration for RNA isolation. .. SE proteins were produced by S. aureus strains grown in brain heart infusion broth supplemented with 1% yeast extract at 37°C for 48 h with aeration ( ).

Article Title: Identification and Characterization of a Novel Staphylococcal Emetic Toxin
Article Snippet: S. aureus was grown in Trypticase soy broth (Nissui Pharmaceutical Co., Tokyo, Japan) at 37°C with aeration for total DNA isolation. .. Escherichia coli strains were grown in LB broth (Sigma-Aldrich, St. Louis, MO) containing 100 μg/ml of ampicillin (Wako Pure Chemicals, Osaka, Japan) for plasmid isolation.

Article Title: Seasonal Change in Bacterial Flora and Biomass in Mountain Snow from the Tateyama Mountains, Japan, Analyzed by 16S rRNA Gene Sequencing and Real-Time PCR
Article Snippet: C. psychrophilum was cultured in Trypticase soy broth (Nissui, Tokyo, Japan) for 24 h at 4°C with shaking (80 rpm), and J. lividum and V. paradoxus were cultured in nutrient broth A medium (5.0 g of peptone/liter, 3.0 g of beef extract/liter, and 3.0 g of NaCl/liter) for 12 h at 25°C with shaking (80 rpm). .. The bacterial DNA was isolated from each culture by use of a FastDNA spin kit for soil in conjunction with a FastPrep FP120 instrument.

Mouse Assay:

Article Title: Immunostimulating Properties of Intragastrically Administered Acetobacter-Derived Soluble Branched (1,4)-?-d-Glucans Decrease Murine Susceptibility to Listeria monocytogenes
Article Snippet: Bacterial virulence was maintained by serial passages in C57BL/6 mice. .. Fresh isolates were obtained from infected spleens, grown in tryptic soy broth (Nissui Pharmaceutical Co., Ltd., Tokyo, Japan), washed repeatedly, resuspended in phosphate-buffered saline (PBS), and stored at −70°C in small aliquots.

Purification:

Article Title: Extended-spectrum β-lactamase-producing Escherichia coliisolated from healthy Thoroughbred racehorses in Japan
Article Snippet: Samples were subcultured in trypticase soy broth (Nissui Pharmaceutical Co., Ltd., Tokyo, Japan) and incubated for 24 hr at 37°C. .. In general, for each sample at least three colonies with typical E.coli morphology were picked from MacConkey agar, purified onto trypticase soy agar (Nissui Pharmaceutical Co., Ltd.) supplemented with 1µ g/ml CTX, and incubated for 24 hr at 37°C.

Article Title: Identification and Characterization of a Novel Staphylococcal Emetic Toxin
Article Snippet: Total DNA of S. aureus was purified using a QIAamp DNA minikit (Qiagen, Tokyo, Japan). .. S. aureus was grown in Trypticase soy broth (Nissui Pharmaceutical Co., Tokyo, Japan) at 37°C with aeration for total DNA isolation.

Plasmid Preparation:

Article Title: Identification and Characterization of Two Novel Staphylococcal Enterotoxins, Types S and T ▿
Article Snippet: Fukuoka 5, Fukuoka 6, and Fukuoka 7, which harbored the plasmid pF5, were isolated from foods sampled during the outbreak of food poisoning that occurred in Fukuoka City, Japan. .. S. aureus strains were grown in two media: Trypticase soy broth (Nissui, Tokyo, Japan), kept at 37°C and maintained with aeration for total DNA isolation, and brain heart infusion broth (Difco Laboratories, Detroit, MI) supplemented with 1% yeast extract (Difco) at 37°C with aeration for RNA isolation.

Article Title: Identification and Characterization of a Novel Staphylococcal Emetic Toxin
Article Snippet: S. aureus was grown in Trypticase soy broth (Nissui Pharmaceutical Co., Tokyo, Japan) at 37°C with aeration for total DNA isolation. .. Escherichia coli strains were grown in LB broth (Sigma-Aldrich, St. Louis, MO) containing 100 μg/ml of ampicillin (Wako Pure Chemicals, Osaka, Japan) for plasmid isolation.

Produced:

Article Title: Identification and Characterization of Two Novel Staphylococcal Enterotoxins, Types S and T ▿
Article Snippet: S. aureus strains were grown in two media: Trypticase soy broth (Nissui, Tokyo, Japan), kept at 37°C and maintained with aeration for total DNA isolation, and brain heart infusion broth (Difco Laboratories, Detroit, MI) supplemented with 1% yeast extract (Difco) at 37°C with aeration for RNA isolation. .. SE proteins were produced by S. aureus strains grown in brain heart infusion broth supplemented with 1% yeast extract at 37°C for 48 h with aeration ( ).

Concentration Assay:

Article Title: Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces
Article Snippet: Streptomyces strains were cultured at 28°C in Bennett's/sugar medium [composition: 1 g l−1 yeast extract (Difco Laboratories, Detroit, Michigan), 1 g l−1 meat extract (Kyokuto, Tokyo, Japan), 2 g l−1 NZ amine (Wako Pure Chemical Industries, Osaka, Japan) and 10 g l−1 of the appropriate sugar (pH 7.2)], R2YE medium , YMP/sugar medium , tryptic soy broth (TSB; Nissui, Tokyo, Japan) and minimal medium [composition: 0.5 g l−1 K2 HPO4 , 0.2 g l−1 MgSO4 7H2 O, 0.01 g l−1 FeSO4 7H2 O, 1.0 g l−1 (NH4 )2 SO4 and 10 g l−1 of the appropriate sugar (pH 7.2)]. .. Escherichia coli transformants were selected in media containing a final concentration of 50 µg ml−1 ampicillin (Wako), neomycin (Wako) or apramycin (Sigma‐Aldrich Japan, Tokyo, Japan).

Article Title: Association of Veterinary Third-Generation Cephalosporin Use with the Risk of Emergence of Extended-Spectrum-Cephalosporin Resistance in Escherichia coli from Dairy Cattle in Japan
Article Snippet: Donors and recipients were grown in tryptic soy broth (TSB, Nissui) to the logarithmic phase; they were then mixed in a total volume of 2 mL at a 1∶9 (v/v) ratio, and 2 mL fresh TSB was added. .. Transconjugants were selected on CTF (final concentration, 4 µg/mL)- and rifampicin (final concentration, 64 µg/mL)-containing MH agar.

Marker:

Article Title: Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces
Article Snippet: TA cloning of polymerase chain reaction (PCR)‐generated DNA fragments was done with the help of pMD19 (Takara Shuzo). pUWLFLP carrying an Flp recombinase gene ( ) was used for the construction of a marker‐less mutant. .. Streptomyces strains were cultured at 28°C in Bennett's/sugar medium [composition: 1 g l−1 yeast extract (Difco Laboratories, Detroit, Michigan), 1 g l−1 meat extract (Kyokuto, Tokyo, Japan), 2 g l−1 NZ amine (Wako Pure Chemical Industries, Osaka, Japan) and 10 g l−1 of the appropriate sugar (pH 7.2)], R2YE medium , YMP/sugar medium , tryptic soy broth (TSB; Nissui, Tokyo, Japan) and minimal medium [composition: 0.5 g l−1 K2 HPO4 , 0.2 g l−1 MgSO4 7H2 O, 0.01 g l−1 FeSO4 7H2 O, 1.0 g l−1 (NH4 )2 SO4 and 10 g l−1 of the appropriate sugar (pH 7.2)].

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    Nissui Pharmaceutical trypticase soy agar
    Trypticase Soy Agar, supplied by Nissui Pharmaceutical, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar/product/Nissui Pharmaceutical
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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