trypticase soy agar  (Thermo Fisher)


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    Structured Review

    Thermo Fisher trypticase soy agar
    Trypticase Soy Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar/product/Thermo Fisher
    Average 94 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Complete genome sequence and phenotype microarray analysis of Cronobacter sakazakii SP291: a persistent isolate cultured from a powdered infant formula production facility
    Article Snippet: .. The isolate was cultured routinely in an Isotherm® Forced Convection Laboratory Incubator (Esco GB Ltd., Downton, UK) at 37°C on Trypticase Soy Agar (Oxoid Limited, Hampshire, UK) and stored at −80°C on cryo-beads (Technical Service Consultants Ltd., Lancashire, UK). .. DNA sequencing, annotation, and comparative genomic analysis Total genomic DNA was purified using a DNeasy Blood and Tissue Kit (QIAGEN, Hilden, Germany) following the manufacturer's instructions.

    Article Title: Lactic acid Bacteria isolated from European badgers (Meles meles) reduce the viability and survival of Bacillus Calmette-Guerin (BCG) vaccine and influence the immune response to BCG in a human macrophage model
    Article Snippet: .. The strains E. coli ATCC25922, E. coli K12 [ ] and S. enterica ATCC14028 were propagated in Trypticase Soy Broth/Agar (TSB/TSA, Oxoid) at 37 °C for 24 h. M. smegmatis mc2 155 was also cultured in TSB at 37 °C but supplemented with 0.05% Tween 80 (Sigma Aldrich) and for 48 h. The two M. bovis strains BCG ΔleuD pASOriM XF [ ] and BCG Pasteur [ ] were cultured in Middlebrook 7H9 (Difco) supplemented with 10% Oleic acid albumin dextrose-catalase (OADC) enrichment (BD BBL™), 0.1% Tween 80 (Sigma Aldrich) and 0.2% glycerol (Sigma Aldrich) or Middlebrook 7H11/7H10 (Difco) medium supplemented with 5% OADC and glycerol (2 mL/L) at 37 °C. .. BCG ΔleuD pASOriM XF was previously generated by transforming BCG Pasteur ΔleuD with the mycobacterial episomal vector pASOriM XF that complements the leucine mutation to correct auxotrophy and enable stable expression of GFP under the control of the constitutively expressed promoter pL5X [ ].

    Bla VIM Assay:

    Article Title: Molecular Epidemiology of Acquired-Metallo-?-Lactamase-Producing Bacteria in Poland
    Article Snippet: Paragraph title: Studies of the location of the bla VIM -containing integrons. ... Transconjugants were selected on Trypticase soy agar (Oxoid, Basingstoke, United Kingdom) plates supplemented with rifampin (300 μg/ml; Polfa Tarchomin, Warsaw, Poland) and imipenem (8 μg/ml; Merck Sharp & Dohme, Rahway, N.J.).

    Expressing:

    Article Title: Lactic acid Bacteria isolated from European badgers (Meles meles) reduce the viability and survival of Bacillus Calmette-Guerin (BCG) vaccine and influence the immune response to BCG in a human macrophage model
    Article Snippet: The strains E. coli ATCC25922, E. coli K12 [ ] and S. enterica ATCC14028 were propagated in Trypticase Soy Broth/Agar (TSB/TSA, Oxoid) at 37 °C for 24 h. M. smegmatis mc2 155 was also cultured in TSB at 37 °C but supplemented with 0.05% Tween 80 (Sigma Aldrich) and for 48 h. The two M. bovis strains BCG ΔleuD pASOriM XF [ ] and BCG Pasteur [ ] were cultured in Middlebrook 7H9 (Difco) supplemented with 10% Oleic acid albumin dextrose-catalase (OADC) enrichment (BD BBL™), 0.1% Tween 80 (Sigma Aldrich) and 0.2% glycerol (Sigma Aldrich) or Middlebrook 7H11/7H10 (Difco) medium supplemented with 5% OADC and glycerol (2 mL/L) at 37 °C. .. BCG ΔleuD pASOriM XF was previously generated by transforming BCG Pasteur ΔleuD with the mycobacterial episomal vector pASOriM XF that complements the leucine mutation to correct auxotrophy and enable stable expression of GFP under the control of the constitutively expressed promoter pL5X [ ].

    Mutagenesis:

    Article Title: Lactic acid Bacteria isolated from European badgers (Meles meles) reduce the viability and survival of Bacillus Calmette-Guerin (BCG) vaccine and influence the immune response to BCG in a human macrophage model
    Article Snippet: The strains E. coli ATCC25922, E. coli K12 [ ] and S. enterica ATCC14028 were propagated in Trypticase Soy Broth/Agar (TSB/TSA, Oxoid) at 37 °C for 24 h. M. smegmatis mc2 155 was also cultured in TSB at 37 °C but supplemented with 0.05% Tween 80 (Sigma Aldrich) and for 48 h. The two M. bovis strains BCG ΔleuD pASOriM XF [ ] and BCG Pasteur [ ] were cultured in Middlebrook 7H9 (Difco) supplemented with 10% Oleic acid albumin dextrose-catalase (OADC) enrichment (BD BBL™), 0.1% Tween 80 (Sigma Aldrich) and 0.2% glycerol (Sigma Aldrich) or Middlebrook 7H11/7H10 (Difco) medium supplemented with 5% OADC and glycerol (2 mL/L) at 37 °C. .. BCG ΔleuD pASOriM XF was previously generated by transforming BCG Pasteur ΔleuD with the mycobacterial episomal vector pASOriM XF that complements the leucine mutation to correct auxotrophy and enable stable expression of GFP under the control of the constitutively expressed promoter pL5X [ ].

    Isolation:

    Article Title: New PHA products using unrelated carbon sources
    Article Snippet: Bacterial strain and growth conditions The soil isolated strains I29B and SA3J ( ) were maintained on solid ISP9 medium ( , ) supplemented with 10 g/l glucose or 10 g/l glucose with 1g/l casein as carbon sources. .. The cultures were incubated at 30 °C during 24–72 h. For cell wall fatty acids composition, strains were cultivated on Trypticase Soy Broth Agar, which consists of 30 g/l Trypticase Soy Broth (Oxoid) and 15 g/l of agar, during 24 h at 28 °C.

    Article Title: Intersection of phosphate transport, oxidative stress and TOR signalling in Candida albicans virulence
    Article Snippet: C . albicans strains were incubated with freshly isolated human neutrophils for the indicated time points at 37°C with 5% CO2 . .. To determine CFU/ml, cells were spread on Trypticase Soy Agar with 5% Sheep Blood (Thermo Fisher Scientific, Cat. # B21239X) or on laboratory-made YPD plates and colonies were counted after incubation at room temperature for 2 days.

    Article Title: Quantitative Microbiological Study of Human Carious Dentine by Culture and Real-Time PCR: Association of Anaerobes with Histopathological Changes in Chronic Pulpitis
    Article Snippet: The carious dentine fragments were dispersed in RTF by first vortexing the fragments for 20 s and then homogenizing by hand in a 2-ml glass homogenizer for 30 s. Serial dilutions (10−3 to 10−6 ) of these suspensions were prepared in RTF; and 100-μl samples were plated in duplicate and incubated either in an anaerobic chamber for up to 2 weeks or, for microaerophilic conditions, in an anaerobic jar with a CO2 gas pack (Oxoid, Basingstoke, United Kingdom) at 37° C for 48 h. The total microbial load per milligram (wet weight) of dentine was determined by measurement of the number of CFU on Trypticase soy agar (Oxoid) containing 1 μg of menadione ml−1 , 0.5 μg of hemin ml−1 , 400 μg of l -cysteine ml−1 , and 5% horse blood (Amyl Media, Kings Langley, New South Wales, Australia) under both anaerobic and microaerophilic conditions (U.S. Department of Health and Human Services, 1982). .. Cadmium fluoride acriflavin tellurite (CFAT) agar containing 13 μg of cadmium sulfate ml−1 , 85 μg of sodium fluoride ml−1 , 1.2 μg of neutral acriflavin ml−1 , 2.5 μg of potassium tellurite ml−1 , and 5% horse blood was used for the selective isolation of Actinomyces spp. and related gram-positive filamentous organisms under anaerobic conditions ( ).

    Article Title: Characterization of Mechanisms Lowering Susceptibility to Flumequine among Bacteria Isolated from Chilean Salmonid Farms
    Article Snippet: Isolates recovered from lake-based salmonid cultures were isolated from samples of mucus and intestinal content of healthy reared fingerlings, surface water samples from salmon cages, and samples of sediments beneath salmonid cages. .. Isolates were stored at −85 °C in CryoBankTM vials (Mast Diagnostica, Reinfeld, Germany) and were grown in Trypticase soy agar (Oxoid, Hants, UK) at 22 °C for 24 h prior to use.

    Article Title: Edwardsiellosis Caused by Edwardsiella ictaluri in Laboratory Populations of Zebrafish Danio rerio
    Article Snippet: .. Standard necropsy procedures included the following: observation of fish externally and internally for gross clinical signs, observation of gill clippings and skin scrapings microscopically in wet mounts for bacteria and parasites, aseptic dissection and collection of tissues (spleen, anterior kidney, and liver), and streaking tissue homogenates on trypticase soy agar with 5% sheep blood (SBA) (Remel, Lenexa, Kansas) for bacterial isolation. .. Primary isolation plates were incubated at 28°C for 48 h. Isolated colonies were subcultured to fresh SBA plates for maintenance of stock cultures prior to inoculation of test media.

    Dissection:

    Article Title: Edwardsiellosis Caused by Edwardsiella ictaluri in Laboratory Populations of Zebrafish Danio rerio
    Article Snippet: .. Standard necropsy procedures included the following: observation of fish externally and internally for gross clinical signs, observation of gill clippings and skin scrapings microscopically in wet mounts for bacteria and parasites, aseptic dissection and collection of tissues (spleen, anterior kidney, and liver), and streaking tissue homogenates on trypticase soy agar with 5% sheep blood (SBA) (Remel, Lenexa, Kansas) for bacterial isolation. .. Primary isolation plates were incubated at 28°C for 48 h. Isolated colonies were subcultured to fresh SBA plates for maintenance of stock cultures prior to inoculation of test media.

    Incubation:

    Article Title: New PHA products using unrelated carbon sources
    Article Snippet: .. The cultures were incubated at 30 °C during 24–72 h. For cell wall fatty acids composition, strains were cultivated on Trypticase Soy Broth Agar, which consists of 30 g/l Trypticase Soy Broth (Oxoid) and 15 g/l of agar, during 24 h at 28 °C. ..

    Article Title: Intersection of phosphate transport, oxidative stress and TOR signalling in Candida albicans virulence
    Article Snippet: .. To determine CFU/ml, cells were spread on Trypticase Soy Agar with 5% Sheep Blood (Thermo Fisher Scientific, Cat. # B21239X) or on laboratory-made YPD plates and colonies were counted after incubation at room temperature for 2 days. .. Isolated human neutrophils were tested in HBSS medium (GIBCO, Cat. # 14025076).

    Article Title: Quantitative Microbiological Study of Human Carious Dentine by Culture and Real-Time PCR: Association of Anaerobes with Histopathological Changes in Chronic Pulpitis
    Article Snippet: .. The carious dentine fragments were dispersed in RTF by first vortexing the fragments for 20 s and then homogenizing by hand in a 2-ml glass homogenizer for 30 s. Serial dilutions (10−3 to 10−6 ) of these suspensions were prepared in RTF; and 100-μl samples were plated in duplicate and incubated either in an anaerobic chamber for up to 2 weeks or, for microaerophilic conditions, in an anaerobic jar with a CO2 gas pack (Oxoid, Basingstoke, United Kingdom) at 37° C for 48 h. The total microbial load per milligram (wet weight) of dentine was determined by measurement of the number of CFU on Trypticase soy agar (Oxoid) containing 1 μg of menadione ml−1 , 0.5 μg of hemin ml−1 , 400 μg of l -cysteine ml−1 , and 5% horse blood (Amyl Media, Kings Langley, New South Wales, Australia) under both anaerobic and microaerophilic conditions (U.S. Department of Health and Human Services, 1982). ..

    Article Title: Characterization of Mechanisms Lowering Susceptibility to Flumequine among Bacteria Isolated from Chilean Salmonid Farms
    Article Snippet: Isolates were previously recovered using plates with Tryptic soy agar (Difco labs) containing oxytetracycline (30 μg/mL) or florfenicol (30 μg/mL) and incubated at 22 °C for 5 days. .. Isolates were stored at −85 °C in CryoBankTM vials (Mast Diagnostica, Reinfeld, Germany) and were grown in Trypticase soy agar (Oxoid, Hants, UK) at 22 °C for 24 h prior to use.

    Article Title: Edwardsiellosis Caused by Edwardsiella ictaluri in Laboratory Populations of Zebrafish Danio rerio
    Article Snippet: Standard necropsy procedures included the following: observation of fish externally and internally for gross clinical signs, observation of gill clippings and skin scrapings microscopically in wet mounts for bacteria and parasites, aseptic dissection and collection of tissues (spleen, anterior kidney, and liver), and streaking tissue homogenates on trypticase soy agar with 5% sheep blood (SBA) (Remel, Lenexa, Kansas) for bacterial isolation. .. Primary isolation plates were incubated at 28°C for 48 h. Isolated colonies were subcultured to fresh SBA plates for maintenance of stock cultures prior to inoculation of test media.

    Article Title: Bacillus cereus strain isolated from Demodexfolliculorum in patients with topical steroid-induced rosaceiform facial dermatitis *
    Article Snippet: .. The sample for bacterial culture was spun two minutes and plated (trypticase soy agar and Columbia agar with 5% sheep's blood (Oxoid – Thermo Fischer, UK), then incubated at 37ºC for 48 hours. .. Identification was performed by microorganisms grown method MALDI-TOF mass spectrometry (Matrix Assisted Laser Desorption Time-of-Flight ionization - Bruker Daltonik GmbH, Bremen, Germany).

    Microscopy:

    Article Title: Bacillus cereus strain isolated from Demodexfolliculorum in patients with topical steroid-induced rosaceiform facial dermatitis *
    Article Snippet: For the microscopic examination, samples were harvested from the affected area using a piece of Scotch transparent tape measuring 2cm, which was subsequently displayed in a microscope slide and examined under optical microscopy (10x-40x Zeiss). .. The sample for bacterial culture was spun two minutes and plated (trypticase soy agar and Columbia agar with 5% sheep's blood (Oxoid – Thermo Fischer, UK), then incubated at 37ºC for 48 hours.

    Sampling:

    Article Title: Among-Population Variation in Microbial Community Structure in the Floral Nectar of the Bee-Pollinated Forest Herb Pulmonaria officinalis L
    Article Snippet: Paragraph title: Study Region and Nectar Sampling ... Subsequently, fifty microliters was plated on both trypticase soy agar (TSA; Oxoid) and yeast extract peptone dextrose agar (YPDA; Difco), representing a general growth medium for bacteria and yeasts, respectively.

    Plasmid Preparation:

    Article Title: Lactic acid Bacteria isolated from European badgers (Meles meles) reduce the viability and survival of Bacillus Calmette-Guerin (BCG) vaccine and influence the immune response to BCG in a human macrophage model
    Article Snippet: The strains E. coli ATCC25922, E. coli K12 [ ] and S. enterica ATCC14028 were propagated in Trypticase Soy Broth/Agar (TSB/TSA, Oxoid) at 37 °C for 24 h. M. smegmatis mc2 155 was also cultured in TSB at 37 °C but supplemented with 0.05% Tween 80 (Sigma Aldrich) and for 48 h. The two M. bovis strains BCG ΔleuD pASOriM XF [ ] and BCG Pasteur [ ] were cultured in Middlebrook 7H9 (Difco) supplemented with 10% Oleic acid albumin dextrose-catalase (OADC) enrichment (BD BBL™), 0.1% Tween 80 (Sigma Aldrich) and 0.2% glycerol (Sigma Aldrich) or Middlebrook 7H11/7H10 (Difco) medium supplemented with 5% OADC and glycerol (2 mL/L) at 37 °C. .. BCG ΔleuD pASOriM XF was previously generated by transforming BCG Pasteur ΔleuD with the mycobacterial episomal vector pASOriM XF that complements the leucine mutation to correct auxotrophy and enable stable expression of GFP under the control of the constitutively expressed promoter pL5X [ ].

    Generated:

    Article Title: Lactic acid Bacteria isolated from European badgers (Meles meles) reduce the viability and survival of Bacillus Calmette-Guerin (BCG) vaccine and influence the immune response to BCG in a human macrophage model
    Article Snippet: The strains E. coli ATCC25922, E. coli K12 [ ] and S. enterica ATCC14028 were propagated in Trypticase Soy Broth/Agar (TSB/TSA, Oxoid) at 37 °C for 24 h. M. smegmatis mc2 155 was also cultured in TSB at 37 °C but supplemented with 0.05% Tween 80 (Sigma Aldrich) and for 48 h. The two M. bovis strains BCG ΔleuD pASOriM XF [ ] and BCG Pasteur [ ] were cultured in Middlebrook 7H9 (Difco) supplemented with 10% Oleic acid albumin dextrose-catalase (OADC) enrichment (BD BBL™), 0.1% Tween 80 (Sigma Aldrich) and 0.2% glycerol (Sigma Aldrich) or Middlebrook 7H11/7H10 (Difco) medium supplemented with 5% OADC and glycerol (2 mL/L) at 37 °C. .. BCG ΔleuD pASOriM XF was previously generated by transforming BCG Pasteur ΔleuD with the mycobacterial episomal vector pASOriM XF that complements the leucine mutation to correct auxotrophy and enable stable expression of GFP under the control of the constitutively expressed promoter pL5X [ ].

    other:

    Article Title: Isolation and Characterization of a Helicobacter sp. from the Gastric Mucosa of Dolphins, Lagenorhynchus acutus and Delphinus delphis
    Article Snippet: Media used for culture were Trypticase soy agar with 5% sheep blood and TVP (trimethoprim, vancomycin, and polymyxin) and CVA (cefoperazone, vancomycin, and amphotericin B) antibiotic-impregnated media (Remel Laboratories, Lenexa, Kans.).

    Article Title: Duration and Clinical Relevance of Postantibiotic Effect in Relation to the Dosing Interval
    Article Snippet: All bacterial samples were plated on Trypticase Soy Agar (TSA; Oxoid, Basingstoke, Hampshire, England).

    Labeling:

    Article Title: Molecular Epidemiology of Acquired-Metallo-?-Lactamase-Producing Bacteria in Poland
    Article Snippet: Probe labeling, hybridization, and signal detection were performed with the ECL Random-Prime Labeling and Detection system (Amersham Pharmacia Biotech). .. Transconjugants were selected on Trypticase soy agar (Oxoid, Basingstoke, United Kingdom) plates supplemented with rifampin (300 μg/ml; Polfa Tarchomin, Warsaw, Poland) and imipenem (8 μg/ml; Merck Sharp & Dohme, Rahway, N.J.).

    Mass Spectrometry:

    Article Title: Bacillus cereus strain isolated from Demodexfolliculorum in patients with topical steroid-induced rosaceiform facial dermatitis *
    Article Snippet: The sample for bacterial culture was spun two minutes and plated (trypticase soy agar and Columbia agar with 5% sheep's blood (Oxoid – Thermo Fischer, UK), then incubated at 37ºC for 48 hours. .. Identification was performed by microorganisms grown method MALDI-TOF mass spectrometry (Matrix Assisted Laser Desorption Time-of-Flight ionization - Bruker Daltonik GmbH, Bremen, Germany).

    Polymerase Chain Reaction:

    Article Title: Molecular Epidemiology of Acquired-Metallo-?-Lactamase-Producing Bacteria in Poland
    Article Snippet: Following exposure, the probe was washed out from the membrane, which was then rehybridized with the 16S-23S rRNA gene probe, obtained in a PCR as previously described ( ). .. Transconjugants were selected on Trypticase soy agar (Oxoid, Basingstoke, United Kingdom) plates supplemented with rifampin (300 μg/ml; Polfa Tarchomin, Warsaw, Poland) and imipenem (8 μg/ml; Merck Sharp & Dohme, Rahway, N.J.).

    Convection:

    Article Title: Complete genome sequence and phenotype microarray analysis of Cronobacter sakazakii SP291: a persistent isolate cultured from a powdered infant formula production facility
    Article Snippet: .. The isolate was cultured routinely in an Isotherm® Forced Convection Laboratory Incubator (Esco GB Ltd., Downton, UK) at 37°C on Trypticase Soy Agar (Oxoid Limited, Hampshire, UK) and stored at −80°C on cryo-beads (Technical Service Consultants Ltd., Lancashire, UK). .. DNA sequencing, annotation, and comparative genomic analysis Total genomic DNA was purified using a DNeasy Blood and Tissue Kit (QIAGEN, Hilden, Germany) following the manufacturer's instructions.

    Hybridization:

    Article Title: Molecular Epidemiology of Acquired-Metallo-?-Lactamase-Producing Bacteria in Poland
    Article Snippet: Probe labeling, hybridization, and signal detection were performed with the ECL Random-Prime Labeling and Detection system (Amersham Pharmacia Biotech). .. Transconjugants were selected on Trypticase soy agar (Oxoid, Basingstoke, United Kingdom) plates supplemented with rifampin (300 μg/ml; Polfa Tarchomin, Warsaw, Poland) and imipenem (8 μg/ml; Merck Sharp & Dohme, Rahway, N.J.).

    Fluorescence In Situ Hybridization:

    Article Title: Characterization of Mechanisms Lowering Susceptibility to Flumequine among Bacteria Isolated from Chilean Salmonid Farms
    Article Snippet: Isolates from land-based culture centers were isolated from various sources including unmedicated fish food pellets, mucus of healthy salmonid fingerlings, and water samples from fish farm influents, effluents, and fish rearing tanks. .. Isolates were stored at −85 °C in CryoBankTM vials (Mast Diagnostica, Reinfeld, Germany) and were grown in Trypticase soy agar (Oxoid, Hants, UK) at 22 °C for 24 h prior to use.

    Article Title: Edwardsiellosis Caused by Edwardsiella ictaluri in Laboratory Populations of Zebrafish Danio rerio
    Article Snippet: .. Standard necropsy procedures included the following: observation of fish externally and internally for gross clinical signs, observation of gill clippings and skin scrapings microscopically in wet mounts for bacteria and parasites, aseptic dissection and collection of tissues (spleen, anterior kidney, and liver), and streaking tissue homogenates on trypticase soy agar with 5% sheep blood (SBA) (Remel, Lenexa, Kansas) for bacterial isolation. .. Primary isolation plates were incubated at 28°C for 48 h. Isolated colonies were subcultured to fresh SBA plates for maintenance of stock cultures prior to inoculation of test media.

    Derivative Assay:

    Article Title: Intersection of phosphate transport, oxidative stress and TOR signalling in Candida albicans virulence
    Article Snippet: HL-60 derived neutrophils were tested at a 20:1 phagocyte:fungus ratio in RPMI plus 10% pooled human serum. .. To determine CFU/ml, cells were spread on Trypticase Soy Agar with 5% Sheep Blood (Thermo Fisher Scientific, Cat. # B21239X) or on laboratory-made YPD plates and colonies were counted after incubation at room temperature for 2 days.

    Software:

    Article Title: Bacillus cereus strain isolated from Demodexfolliculorum in patients with topical steroid-induced rosaceiform facial dermatitis *
    Article Snippet: The sample for bacterial culture was spun two minutes and plated (trypticase soy agar and Columbia agar with 5% sheep's blood (Oxoid – Thermo Fischer, UK), then incubated at 37ºC for 48 hours. .. Measurements were performed using MALDI-TOF MS Microflex LT through the FlexControl software.

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    Thermo Fisher trypticase soy agar
    Trypticase Soy Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar/product/Thermo Fisher
    Average 94 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
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      Buy from Supplier

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    Thermo Fisher trypticase soy agar tsa
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