Structured Review

Merck KGaA trypticase soy agar
Trypticase Soy Agar, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trypticase soy agar/product/Merck KGaA
Average 93 stars, based on 2 article reviews
Price from $9.99 to $1999.99
trypticase soy agar - by Bioz Stars, 2020-04
93/100 stars

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Related Articles

DNA Extraction:

Article Title: Virulence factors, serogroups and antimicrobial resistance properties of Escherichia coli strains in fermented dairy products
Article Snippet: .. DNA extraction Bacterial strains were grown overnight in Trypticase Soy Agar (TSA, Merck, Germany) at 37°C. ..

Article Title: Serogroups, virulence genes and antibiotic resistance in Shiga toxin-producing Escherichia coli isolated from diarrheic and non-diarrheic pediatric patients in Iran
Article Snippet: .. DNA extraction Bacterial strains were grown overnight in Trypticase Soy Agar (TSA, Merck, Germany) at 37°C. ..

Flow Cytometry:

Article Title: MIR spectroscopy as alternative method for further confirmation of foodborne pathogens Salmonella spp. and Listeria monocytogenes
Article Snippet: Bacterial colonies grew in agar medium, for 18 h at 37 °C: trypticase soy agar (TSA, Merck, Germany) for Salmonella Nottingham and L. monocytogenes preliminary analysis, XLD for Salmonella , Shigella, Citrobacter and Pseudomonas and OCLA for Listeria . .. The colonies were collected with a loop and placed directly on the crystal of a 2 mm × 2 mm horizontal single attenuated total reflectance (ATR) accessory and were dried under gentle cold air flow for 10 s. At least three replicate spectra were obtained for each sample, obtained from different plates in different days.

Amplification:

Article Title: Virulence factors, serogroups and antimicrobial resistance properties of Escherichia coli strains in fermented dairy products
Article Snippet: DNA extraction Bacterial strains were grown overnight in Trypticase Soy Agar (TSA, Merck, Germany) at 37°C. .. The supernatant was used as a template for PCR amplification.

Article Title: Serogroups, virulence genes and antibiotic resistance in Shiga toxin-producing Escherichia coli isolated from diarrheic and non-diarrheic pediatric patients in Iran
Article Snippet: DNA extraction Bacterial strains were grown overnight in Trypticase Soy Agar (TSA, Merck, Germany) at 37°C. .. The supernatant was used as a template for PCR amplification.

In Vitro:

Article Title: Biosurfactant Produced by Salmonella Enteritidis SE86 Can Increase Adherence and Resistance to Sanitizers on Lettuce Leaves (Lactuca sativa L., cichoraceae)
Article Snippet: Paragraph title: Resistance to Sanitizers of S. Enteritidis SE86 With and Without Biosurfactant In Vitro ... The negative confirmation of results (tubes without growth) was performed through inoculation on trypticase soy agar (TSA agar, Merck, Darmstadt, Germany) incubated at 36 ± 1°C for 24 h. Each experiment was performed in triplicate on different days.

Tube Formation Assay:

Article Title: Eradication of Pseudomonas aeruginosa Biofilms Using the Combination of n-butanolic Cyclamen coum Extract and Ciprofloxacin
Article Snippet: Paragraph title: 3.2. Biofilm Formation Assay ... An overnight culture of each strain was grown in trypticase soy agar plus 0.2% glucose (Merck, Germany) for 20 hours at 37°C.

Isolation:

Article Title: Laparotomy and laparoscopy diversely affect macrophage-associated antimicrobial activity in a murine model
Article Snippet: Isolated peritoneal macrophages were chased with live E. coli or S. aureus (ATCC, Manassas, VA) at 37°C with a macrophage/bacteria ratio of 1:20 for 10 min, and this time point was marked as time 0. .. Serial 10-fold dilutions of the cell lysates were plated on lysogeny broth agar (Sigma-Aldrich) or trypticase soy agar (Merck, Darmstadt, Germany), and cultured for 24 h at 37°C for determination of bacterial CFU.

Article Title: Toxigenic Vibrio cholerae O1 in vegetables and fish raised in wastewater irrigated fields and stabilization ponds during a non-cholera outbreak period in Morogoro, Tanzania: an environmental health study
Article Snippet: Paragraph title: Isolation of Vibrio cholerae ... Following 24 h incubation at 37 °C, yellow colonies (sucrose fermenting, 2–3 mm diameter) suspected as Vibrio cholerae were purified on trypticase soy agar (Merck, Darmstadt, Germany) for 24 h at 37 °C.

Purification:

Article Title: Toxigenic Vibrio cholerae O1 in vegetables and fish raised in wastewater irrigated fields and stabilization ponds during a non-cholera outbreak period in Morogoro, Tanzania: an environmental health study
Article Snippet: .. Following 24 h incubation at 37 °C, yellow colonies (sucrose fermenting, 2–3 mm diameter) suspected as Vibrio cholerae were purified on trypticase soy agar (Merck, Darmstadt, Germany) for 24 h at 37 °C. .. Purified colonies were characterized by Gram staining (Gram-negative comma shaped rods), positive oxidase reaction (colour change to blue dark within 5 s) and reaction in triple sugar iron agar (yellow slants with no gas formation) [ ].

Spectrophotometry:

Article Title: Eradication of Pseudomonas aeruginosa Biofilms Using the Combination of n-butanolic Cyclamen coum Extract and Ciprofloxacin
Article Snippet: An overnight culture of each strain was grown in trypticase soy agar plus 0.2% glucose (Merck, Germany) for 20 hours at 37°C. .. The bacterial suspensions were prepared in trypticase soy broth plus 0.2% glucose medium according to 0.5 McFarland turbidity standard as measured by absorbance (0.08 - 0.1 at 625 nm) using a spectrophotometer (Schimadzu, model UV-120-01, Japan).

Mouse Assay:

Article Title: Laparotomy and laparoscopy diversely affect macrophage-associated antimicrobial activity in a murine model
Article Snippet: Serial 10-fold dilutions of the cell lysates were plated on lysogeny broth agar (Sigma-Aldrich) or trypticase soy agar (Merck, Darmstadt, Germany), and cultured for 24 h at 37°C for determination of bacterial CFU. .. Peritoneal macrophages isolated from the control mice and mice that underwent either laparotomy or laparoscopy 24 h after the surgical procedures were pretreated with 50 μM 1400 W (N-(3-(Aminomethyl)benzyl)acetamidine) (Sigma-Aldrich), a highly selective inducible nitric oxide synthase (iNOS) inhibitor for 4 h, and then chased with live E. coli or S. aureus at 37°C with a macrophage/bacteria ratio of 1:20 for 10 min.

Modification:

Article Title: Eradication of Pseudomonas aeruginosa Biofilms Using the Combination of n-butanolic Cyclamen coum Extract and Ciprofloxacin
Article Snippet: Biofilm Formation Assay A modified microtiter plate test was used to determine the biofilm formation. .. An overnight culture of each strain was grown in trypticase soy agar plus 0.2% glucose (Merck, Germany) for 20 hours at 37°C.

Incubation:

Article Title: Eradication of Pseudomonas aeruginosa Biofilms Using the Combination of n-butanolic Cyclamen coum Extract and Ciprofloxacin
Article Snippet: An overnight culture of each strain was grown in trypticase soy agar plus 0.2% glucose (Merck, Germany) for 20 hours at 37°C. .. Bacterial suspensions (200 μL) were aliquoted in to a 96-well polystyrene microtiter plate (Sigma Aldrich, St. Louis, Missouri, USA) and incubated at 37°C for 24 hours without agitation.

Article Title: Biosurfactant Produced by Salmonella Enteritidis SE86 Can Increase Adherence and Resistance to Sanitizers on Lettuce Leaves (Lactuca sativa L., cichoraceae)
Article Snippet: .. The negative confirmation of results (tubes without growth) was performed through inoculation on trypticase soy agar (TSA agar, Merck, Darmstadt, Germany) incubated at 36 ± 1°C for 24 h. Each experiment was performed in triplicate on different days. ..

Article Title: Disarming Pseudomonas aeruginosa Virulence by the Inhibitory Action of 1,10-Phenanthroline-5,6-Dione-Based Compounds: Elastase B (LasB) as a Chemotherapeutic Target
Article Snippet: Planktonic- and Biofilm-Growing P. aeruginosa Cells P. aeruginosa cells were grown on trypticase soy agar (TSA; Merck, Darmstadt, Germany) for 18 h at 37°C. .. Subsequently, planktonic bacterial cells were subcultured in tryptone soy broth (TSB) supplemented with 1% glycerol, 50 mM glutamate, 10 mM CaCl2 and 10 mM ZnCl2 and incubated for 24 h at 37°C under constant agitation ( ).

Article Title: Characterization of Airborne Bacteria at an Underground Subway Station
Article Snippet: To enumerate total airborne cultivable bacteria, filter extracts (100 μl) were plated in triplicate using a standard spread plate method onto Trypticase soy agar (TSA; Merck, Darmstadt, Germany) and Reasoner's 2a (R2A) (Oxoid, Cambridge, United Kingdom) plates, supplemented with 100 μg of cycloheximide (Sigma-Aldrich, St. Louis, MO) ml−1 to avoid fungal growth ( ). .. Incubation was performed at 30°C for 48 h before colony counting.

Article Title: Burst Release of Antibiotics Combined with Long-Term Release of Silver Targeting Implant-Associated Infections: Design, Characterization and in vitro Evaluation of Novel Implant Hybrid Surface
Article Snippet: .. The G hybrid K-wire pieces used to assess gentamicin release over time were placed without any further preparation on 1.5% tryptic soy broth agar (Merck, Darmstadt; Germany), which was inoculated with 100 µL S. cohnii (2.25 × 107 CFU/mL), and incubated for 24 h at 37 °C. .. The inhibition zone was measured using ImageJ software (Version 1.52a, Maryland, United States of America) and converted to ideal circle diameter.

Article Title: Toxigenic Vibrio cholerae O1 in vegetables and fish raised in wastewater irrigated fields and stabilization ponds during a non-cholera outbreak period in Morogoro, Tanzania: an environmental health study
Article Snippet: .. Following 24 h incubation at 37 °C, yellow colonies (sucrose fermenting, 2–3 mm diameter) suspected as Vibrio cholerae were purified on trypticase soy agar (Merck, Darmstadt, Germany) for 24 h at 37 °C. .. Purified colonies were characterized by Gram staining (Gram-negative comma shaped rods), positive oxidase reaction (colour change to blue dark within 5 s) and reaction in triple sugar iron agar (yellow slants with no gas formation) [ ].

other:

Article Title: Antibacterial and Antioxidant Activities of Acid and Bile Resistant Strains of Lactobacillus fermentum Isolated from Miang
Article Snippet: They were maintained by routine culture on trypticase soy agar (TSA, Merck, Germany) with 0.5% yeast extract and were stored in trypticase soy broth (TSB, Merck, Darmstadt, Germany) containing 20% glycerol at -20°C.

Article Title: Surface Grafted MSI-78A Antimicrobial Peptide has High Potential for Gastric Infection Management
Article Snippet: Bacteria were grown on Trypticase Soy Agar (TSA; Merck Millipore) plates overnight at 37 °C (spreading) and then transferred to Trypticase Soy Broth (TSB; Merck Millipore).

Cell Culture:

Article Title: Laparotomy and laparoscopy diversely affect macrophage-associated antimicrobial activity in a murine model
Article Snippet: .. Serial 10-fold dilutions of the cell lysates were plated on lysogeny broth agar (Sigma-Aldrich) or trypticase soy agar (Merck, Darmstadt, Germany), and cultured for 24 h at 37°C for determination of bacterial CFU. ..

Spectroscopy:

Article Title: MIR spectroscopy as alternative method for further confirmation of foodborne pathogens Salmonella spp. and Listeria monocytogenes
Article Snippet: Paragraph title: Mid-infrared spectroscopy ... Bacterial colonies grew in agar medium, for 18 h at 37 °C: trypticase soy agar (TSA, Merck, Germany) for Salmonella Nottingham and L. monocytogenes preliminary analysis, XLD for Salmonella , Shigella, Citrobacter and Pseudomonas and OCLA for Listeria .

Lysis:

Article Title: Laparotomy and laparoscopy diversely affect macrophage-associated antimicrobial activity in a murine model
Article Snippet: After removal of the culture supernatants at each time point, macrophages were washed with PBS and lysed in 0.5 ml lysis buffer containing 0.5% Triton X-100 (Sigma-Aldrich) for 10 min. .. Serial 10-fold dilutions of the cell lysates were plated on lysogeny broth agar (Sigma-Aldrich) or trypticase soy agar (Merck, Darmstadt, Germany), and cultured for 24 h at 37°C for determination of bacterial CFU.

Polymerase Chain Reaction:

Article Title: Virulence factors, serogroups and antimicrobial resistance properties of Escherichia coli strains in fermented dairy products
Article Snippet: DNA extraction Bacterial strains were grown overnight in Trypticase Soy Agar (TSA, Merck, Germany) at 37°C. .. The supernatant was used as a template for PCR amplification.

Article Title: Serogroups, virulence genes and antibiotic resistance in Shiga toxin-producing Escherichia coli isolated from diarrheic and non-diarrheic pediatric patients in Iran
Article Snippet: DNA extraction Bacterial strains were grown overnight in Trypticase Soy Agar (TSA, Merck, Germany) at 37°C. .. The supernatant was used as a template for PCR amplification.

Staining:

Article Title: Toxigenic Vibrio cholerae O1 in vegetables and fish raised in wastewater irrigated fields and stabilization ponds during a non-cholera outbreak period in Morogoro, Tanzania: an environmental health study
Article Snippet: Following 24 h incubation at 37 °C, yellow colonies (sucrose fermenting, 2–3 mm diameter) suspected as Vibrio cholerae were purified on trypticase soy agar (Merck, Darmstadt, Germany) for 24 h at 37 °C. .. Purified colonies were characterized by Gram staining (Gram-negative comma shaped rods), positive oxidase reaction (colour change to blue dark within 5 s) and reaction in triple sugar iron agar (yellow slants with no gas formation) [ ].

Sampling:

Article Title: MIR spectroscopy as alternative method for further confirmation of foodborne pathogens Salmonella spp. and Listeria monocytogenes
Article Snippet: Bacterial colonies grew in agar medium, for 18 h at 37 °C: trypticase soy agar (TSA, Merck, Germany) for Salmonella Nottingham and L. monocytogenes preliminary analysis, XLD for Salmonella , Shigella, Citrobacter and Pseudomonas and OCLA for Listeria . .. The sampling accessory was cleaned with ethanol (70%) and distilled water between each measurement.

Fluorescence In Situ Hybridization:

Article Title: Toxigenic Vibrio cholerae O1 in vegetables and fish raised in wastewater irrigated fields and stabilization ponds during a non-cholera outbreak period in Morogoro, Tanzania: an environmental health study
Article Snippet: For fish samples, subsamples were individually taken from gills and intestines (10 g of each) and added to 90 ml of APW for enrichment before culture. .. Following 24 h incubation at 37 °C, yellow colonies (sucrose fermenting, 2–3 mm diameter) suspected as Vibrio cholerae were purified on trypticase soy agar (Merck, Darmstadt, Germany) for 24 h at 37 °C.

Inhibition:

Article Title: Burst Release of Antibiotics Combined with Long-Term Release of Silver Targeting Implant-Associated Infections: Design, Characterization and in vitro Evaluation of Novel Implant Hybrid Surface
Article Snippet: According to the epidemiology and the limitation regarding risk classification, Staphylococcus cohnii (ATCC 29974) (S. cohnii ) was selected for the efficacy study of the hybrid surface measuring zone of inhibition (ZOI). .. The G hybrid K-wire pieces used to assess gentamicin release over time were placed without any further preparation on 1.5% tryptic soy broth agar (Merck, Darmstadt; Germany), which was inoculated with 100 µL S. cohnii (2.25 × 107 CFU/mL), and incubated for 24 h at 37 °C.

Software:

Article Title: Burst Release of Antibiotics Combined with Long-Term Release of Silver Targeting Implant-Associated Infections: Design, Characterization and in vitro Evaluation of Novel Implant Hybrid Surface
Article Snippet: The G hybrid K-wire pieces used to assess gentamicin release over time were placed without any further preparation on 1.5% tryptic soy broth agar (Merck, Darmstadt; Germany), which was inoculated with 100 µL S. cohnii (2.25 × 107 CFU/mL), and incubated for 24 h at 37 °C. .. The inhibition zone was measured using ImageJ software (Version 1.52a, Maryland, United States of America) and converted to ideal circle diameter.

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  • 93
    Merck KGaA trypticase soy agar
    Trypticase Soy Agar, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar/product/Merck KGaA
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    91
    Merck KGaA trypticase soy agar tsa
    Trypticase Soy Agar Tsa, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar tsa/product/Merck KGaA
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar tsa - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

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