Structured Review

Fisher Scientific trypticase soy agar
Trypticase Soy Agar, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trypticase soy agar/product/Fisher Scientific
Average 93 stars, based on 4 article reviews
Price from $9.99 to $1999.99
trypticase soy agar - by Bioz Stars, 2020-04
93/100 stars

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Isolation:

Article Title: Persistence of Pigment Production by Yeast Isolates Grown on CHROMagar Candida Medium
Article Snippet: A sample of each frozen isolate was grown on plates of Trypticase soy agar with 5% sheep blood (Fisher Scientific, Houston, Tex.) at 30°C. .. Inoculum density was adjusted to a McFarland standard of 2.0 by using a Vitek Colorimeter (HACH Company, Loveland, Colo.), and 1-μl aliquots of various inocula (one species or mixtures of 2 or 3 species) were streaked for isolation onto duplicate sets of 100 CHROMagar Candida plates.

Generated:

Article Title: Contribution of Structural Domains to the Activity of Ribonuclease 7 against Uropathogenic Bacteria
Article Snippet: Uropathogenic S. saprophyticus (ATCC 15305) was grown in LB and plated on Trypticase soy agar with 5% sheep blood (Fisher Scientific, Pittsburgh, PA). .. Anti-RNase 7 antibody was generated in rabbits using recombinant human RNase 7 (Biomatik, Wilmington, DE).

Infection:

Article Title: Capacitive Coupling Reduces Instrumentation-related Infection in Rabbit Spines: A Pilot Study
Article Snippet: The NZW rabbit infection model with multiple surgical sites mimics posterior spinal surgery with instrumentation and is based on a previously described protocol [ , ]. .. The concentration was standardized with a densitometric turbidity meter (LaMotte 2020e; LaMotte Company, Chestertown, MD, USA), which correlated with CFU/100 μL plated on trypticase soy agar plates with 10% sheep blood (Fisher Scientific, Pittsburgh, PA, USA).

Mouse Assay:

Article Title: Evaluation of Extended Sanitation Interval for Cage Top Components in Individually Ventilated Mouse Cages
Article Snippet: A standardized group size of 4 NIH/Swiss male or female mice per cage was used, with a total of 40 test cages and 4 control cages. .. These swabs were applied immediately to 100-mm culture plates containing trypticase soy agar (Fisher Scientific, Waltham MA) in a spread-plate pattern.

Article Title: The innate immune receptor Nod1 protects the intestine from inflammation-induced tumorigenesis
Article Snippet: Due to only modest intestinal depletion as assayed by fecal cultures, mice were subsequently switched over to a different antibiotic cocktail of 2 g/L streptomycin, 0.17 g/L gentamycin, 0.125 mg/L ciprofloxacin and 1g/L bacitracin which was maintained for the duration of the experiment. .. Intestinal depletion was assessed by collecting feces, homogenizing in PBS, serially diluting and plating on trypticase soy agar with 10% sheep blood (Fisher Scientific) for 48 hours at 37° aerobically and in anaerobic chamber (AnaeroPack System).

Article Title: Ferritin Light Chain Confers Protection Against Sepsis-Induced Inflammation and Organ Injury
Article Snippet: Peritoneal Colony Forming Unit (CFU) Measurement Mice were anesthetized with 1.5–2% isoflurane and peritoneal lavage was sterilely collected in 0.9% sterile saline. .. Serial dilutions of the peritoneal lavage fluid were made and plated on Trypticase Soy Agar with Sheep Blood (Fisher Scientific).

Article Title: Keratin 8 reduces colonic permeability and maintains gut microbiota homeostasis, protecting against colitis and colitis-associated tumorigenesis
Article Snippet: Due to only modest intestinal depletion as assayed by fecal cultures, mice were subsequently switched over to a different antibiotic cocktail of 2 g/L streptomycin, 0.17 g/L gentamycin, 0.125 mg/L ciprofloxacin and 1g/L bacitracin which was maintained for the duration of the experiment. .. Intestinal depletion was assessed by collecting feces, homogenizing in PBS, serially diluting and plating on trypticase soy agar with 10% sheep blood (Fisher Scientific) for 48 hours at 37°C aerobically and in anaerobic chamber (AnaeroPack System).

Concentration Assay:

Article Title: Capacitive Coupling Reduces Instrumentation-related Infection in Rabbit Spines: A Pilot Study
Article Snippet: .. The concentration was standardized with a densitometric turbidity meter (LaMotte 2020e; LaMotte Company, Chestertown, MD, USA), which correlated with CFU/100 μL plated on trypticase soy agar plates with 10% sheep blood (Fisher Scientific, Pittsburgh, PA, USA). ..

Article Title: Insights on the Role of Antimicrobial Cuffed Endotracheal Tubes in Preventing Transtracheal Transmission of VAP Pathogens from an In Vitro Model of Microaspiration and Microbial Proliferation
Article Snippet: .. Contaminated Secretions Isolates were freshly grown on trypticase soy agar + 5% sheep blood, inoculated into Mueller-Hinton II broth (Fischer Scientific, Pittsburg, PA) and diluted to a concentration of 5.5 × 105 colony-forming units per milliliter for performing contaminated secretion bacterial challenges. .. This concentration was chosen as a worst case scenario and was greater than the 1 × 104 bronchoalveolar lavage concentration used to define VAP in previous studies [ ].

Article Title: High Throughput Screening for Natural Host Defense Peptide-Inducing Compounds as Novel Alternatives to Antibiotics
Article Snippet: Paragraph title: Minimum inhibitory concentration (MIC) assay ... Briefly, Salmonella enterica subsp. enterica serovar Enteritidis (ATCC 13076) and Escherichia coli (ATCC 25922) were streaked onto trypticase soy agar (Fisher Scientific) plates, followed by subculture of 2–3 individual colonies in trypticase soy broth (Fisher Scientific) with shaking at 37°C for 3 h to reach the mid-log phase.

Incubation:

Article Title: Capacitive Coupling Reduces Instrumentation-related Infection in Rabbit Spines: A Pilot Study
Article Snippet: On the day before surgery, a suspension of S aureus colonies in 5 mL trypticase soy broth was incubated at 37°C overnight for 16 hours. .. The concentration was standardized with a densitometric turbidity meter (LaMotte 2020e; LaMotte Company, Chestertown, MD, USA), which correlated with CFU/100 μL plated on trypticase soy agar plates with 10% sheep blood (Fisher Scientific, Pittsburgh, PA, USA).

Article Title: Evaluation of Extended Sanitation Interval for Cage Top Components in Individually Ventilated Mouse Cages
Article Snippet: These swabs were applied immediately to 100-mm culture plates containing trypticase soy agar (Fisher Scientific, Waltham MA) in a spread-plate pattern. .. After 24 h of incubation at 37 °C, colonies were counted to determine total aerobic microbial growth.

Article Title: High Throughput Screening for Natural Host Defense Peptide-Inducing Compounds as Novel Alternatives to Antibiotics
Article Snippet: Briefly, Salmonella enterica subsp. enterica serovar Enteritidis (ATCC 13076) and Escherichia coli (ATCC 25922) were streaked onto trypticase soy agar (Fisher Scientific) plates, followed by subculture of 2–3 individual colonies in trypticase soy broth (Fisher Scientific) with shaking at 37°C for 3 h to reach the mid-log phase. .. MIC was determined as the lowest concentration of the compound or compound combination that gave no visible bacterial growth after overnight incubation at 37°C.

Article Title: High Throughput Screening for Natural Host Defense Peptide-Inducing Compounds as Novel Alternatives to Antibiotics
Article Snippet: Briefly, Salmonella enterica subsp. enterica serovar Enteritidis (ATCC 13076) and Escherichia coli (ATCC 25922) were streaked onto trypticase soy agar (Fisher Scientific) plates, followed by subculture of 2–3 individual colonies in trypticase soy broth (Fisher Scientific) with shaking at 37°C for 3 h to reach the mid-log phase. .. MIC was determined as the lowest concentration of the compound or compound combination that gave no visible bacterial growth after overnight incubation at 37°C.

Recombinant:

Article Title: Contribution of Structural Domains to the Activity of Ribonuclease 7 against Uropathogenic Bacteria
Article Snippet: Uropathogenic S. saprophyticus (ATCC 15305) was grown in LB and plated on Trypticase soy agar with 5% sheep blood (Fisher Scientific, Pittsburgh, PA). .. Anti-RNase 7 antibody was generated in rabbits using recombinant human RNase 7 (Biomatik, Wilmington, DE).

Microdilution Assay:

Article Title: High Throughput Screening for Natural Host Defense Peptide-Inducing Compounds as Novel Alternatives to Antibiotics
Article Snippet: Minimum inhibitory concentration (MIC) assay The MICs of wortmannin and butyrate were determined using a standard broth microdilution assay as recommended by the Clinical and Laboratory Standards Institute (National Committee for Clinical Laboratory Standards, ) as we previously described (Xiao et al., , ; Bommineni et al., ). .. Briefly, Salmonella enterica subsp. enterica serovar Enteritidis (ATCC 13076) and Escherichia coli (ATCC 25922) were streaked onto trypticase soy agar (Fisher Scientific) plates, followed by subculture of 2–3 individual colonies in trypticase soy broth (Fisher Scientific) with shaking at 37°C for 3 h to reach the mid-log phase.

Article Title: High Throughput Screening for Natural Host Defense Peptide-Inducing Compounds as Novel Alternatives to Antibiotics
Article Snippet: The MICs of wortmannin and butyrate were determined using a standard broth microdilution assay as recommended by the Clinical and Laboratory Standards Institute (National Committee for Clinical Laboratory Standards, ) as we previously described (Xiao et al., , ; Bommineni et al., ). .. Briefly, Salmonella enterica subsp. enterica serovar Enteritidis (ATCC 13076) and Escherichia coli (ATCC 25922) were streaked onto trypticase soy agar (Fisher Scientific) plates, followed by subculture of 2–3 individual colonies in trypticase soy broth (Fisher Scientific) with shaking at 37°C for 3 h to reach the mid-log phase.

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