trypticase soy agar tsa  (Thermo Fisher)


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    Structured Review

    Thermo Fisher trypticase soy agar tsa
    Trypticase Soy Agar Tsa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar tsa/product/Thermo Fisher
    Average 95 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar tsa - by Bioz Stars, 2020-04
    95/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Characterization of surface proteins of Cronobacter muytjensii using monoclonal antibodies and MALDI-TOF Mass spectrometry
    Article Snippet: Bacteria and growth conditions Stock cultures were maintained through out this study on Trypticase Soy Agar (TSA) (Oxoid, UK) or nutrient agar plates (HiMedia, India) at 4°C until use. .. Cells were harvested by centrifugation (5,000 × g, 10 min), washed twice with 0.1 M PBS (pH 7.2) and adjusted to 108 CFU ml-1 using McFarland standard.

    Amplification:

    Article Title: Phosphorylation dependent and independent regulation of Helicobacter pylori acid acclimation by the ArsRS two-component system
    Article Snippet: A non-polar ATCC 43504 ΔarsS deletion mutant was constructed by allelic exchange using a kanamycin resistance gene as described previously, and downstream gene expression in the knockout was confirmed by RT-PCR amplification of the downstream HP0163 gene ( ). .. Bacteria were grown under microaerobic conditions (5% O2 , 10% CO2 , 85% N2 ) either on Trypticase Soy Agar (TSA) plates supplemented with 5% sheep blood (Gibco BRL-Life Technologies) or in brain heart infusion (BHI) medium (Difco Laboratories) supplemented with 7% horse serum (Gibco BRL-Life Technologies) and 0.25% yeast extract (Difco Laboratories).

    Mass Spectrometry:

    Article Title: Accurate and Rapid Differentiation of Acinetobacter baumannii Strains by Raman Spectroscopy: a Comparative Study
    Article Snippet: Paragraph title: Mass spectrometry. ... The isolates were grown on Trypticase soy agar (TSA) with sheep blood agar (catalog number R01201; Remel) for 18 to 24 h at 37°C in a 5% CO2 incubator.

    Construct:

    Article Title: Role of the Helicobacter pylori Sensor Kinase ArsS in Protein Trafficking and Acid Acclimation
    Article Snippet: A nonpolar ATCC 43504 Δ arsS deletion mutant was constructed by allelic exchange, as previously described ( ) and as summarized below. .. Bacteria were grown under microaerobic conditions (5% O2 , 10% CO2 , and 85% N2 ) either on Trypticase soy agar (TSA) plates supplemented with 5% sheep blood (Gibco) or in brain heart infusion (BHI) medium (Difco) supplemented with 7% horse serum (Gibco) and 0.25% yeast extract (Difco).

    Article Title: Phosphorylation dependent and independent regulation of Helicobacter pylori acid acclimation by the ArsRS two-component system
    Article Snippet: An arsR(D52N) mutant was constructed as described below. .. Bacteria were grown under microaerobic conditions (5% O2 , 10% CO2 , 85% N2 ) either on Trypticase Soy Agar (TSA) plates supplemented with 5% sheep blood (Gibco BRL-Life Technologies) or in brain heart infusion (BHI) medium (Difco Laboratories) supplemented with 7% horse serum (Gibco BRL-Life Technologies) and 0.25% yeast extract (Difco Laboratories).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Characterization of surface proteins of Cronobacter muytjensii using monoclonal antibodies and MALDI-TOF Mass spectrometry
    Article Snippet: Bacteria and growth conditions Stock cultures were maintained through out this study on Trypticase Soy Agar (TSA) (Oxoid, UK) or nutrient agar plates (HiMedia, India) at 4°C until use. .. Bacterial cells were heated at 80°C for 20 min in a water bath and were subsequently used for immunization of mice and screening of hybridoma cells for MAbs production using ELISA.

    Incubation:

    Article Title: Postmenopause as a key factor in the composition of the Endometrial Cancer Microbiome (ECbiome)
    Article Snippet: They were subsequently plated on Trypticase soy agar (TSA) with 5% sheep blood (ThermoFisher Scientific, Waltham, MA) (following ATCC guidelines). .. The microbes were incubated at 37 °C in an anaerobic environment for 48 hours for P. somerae and 72 hours for A. vaginae .

    Article Title: Supporting evidence for a human reservoir of invasive non-Typhoidal Salmonella from household samples in Burkina Faso
    Article Snippet: All agar plates were incubated for 12–18 hours at 35–37°C. .. Subcultures of all isolates were shipped to the Institute of Tropical Medicine of Antwerp (ITM) on Trypticase Soy Agar (TSA) (Oxoid) and the Belgian reference center for Salmonella (Sciensano, Brussels) for serotype confirmation, MLVA typing and WGS analysis.

    Activity Assay:

    Article Title: Structure-function analysis of Avian β-defensin-6 and β-defensin-12: role of charge and disulfide bridges
    Article Snippet: Paragraph title: Antimicrobial activity assay ... All bacterial strains were grown and maintained on Trypticase Soy Agar (TSA) with 5 % Sheep Blood (Thermo Fisher Scientific) at 37 °C.

    Expressing:

    Article Title: Phosphorylation dependent and independent regulation of Helicobacter pylori acid acclimation by the ArsRS two-component system
    Article Snippet: A non-polar ATCC 43504 ΔarsS deletion mutant was constructed by allelic exchange using a kanamycin resistance gene as described previously, and downstream gene expression in the knockout was confirmed by RT-PCR amplification of the downstream HP0163 gene ( ). .. Bacteria were grown under microaerobic conditions (5% O2 , 10% CO2 , 85% N2 ) either on Trypticase Soy Agar (TSA) plates supplemented with 5% sheep blood (Gibco BRL-Life Technologies) or in brain heart infusion (BHI) medium (Difco Laboratories) supplemented with 7% horse serum (Gibco BRL-Life Technologies) and 0.25% yeast extract (Difco Laboratories).

    BIA-KA:

    Article Title: Development and Validation of an In Vitro Pharmacokinetic/Pharmacodynamic Model To Test the Antibacterial Efficacy of Antibiotic Polymer Conjugates
    Article Snippet: Sodium hydrogen orthophosphate, potassium dihydrogen orthophosphate, potassium chloride, sodium hydroxide (NaOH), copper (II) sulfate pentahydrate, bicinchoninic acid (BCA) assay kit, and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) were purchased from Thermo Fisher Scientific (Loughborough, United Kingdom). .. Trypticase soy agar (TSA), Trypticase soy broth (TSB), and cation-adjusted Mueller-Hinton broth (CAMHB, lot 43145) were purchased from Oxoid (Basingstoke, United Kingdom).

    Transferring:

    Article Title: Accurate and Rapid Differentiation of Acinetobacter baumannii Strains by Raman Spectroscopy: a Comparative Study
    Article Snippet: The isolates were grown on Trypticase soy agar (TSA) with sheep blood agar (catalog number R01201; Remel) for 18 to 24 h at 37°C in a 5% CO2 incubator. .. A suspension of α-cyano-4-hydroxycinnamic acid (Vitek MS CHCA, bioMérieux SA) was overlaid onto each sample (approximately 1 μl dispensed by pipette).

    Cell Culture:

    Article Title: Genotypic and phenotypic characterization of biofilm production by Staphylococcus aureus strains isolated from bovine intramammary infections in Colombian dairy farms
    Article Snippet: .. 2.2 DNA extraction S. aureus strains stocks were thawed and cultured on Trypticase soy agar (TSA) (Oxoid, United Kingdom). .. DNA was extracted using DNeasy Blood & Tissue kit (Qiagen, Germany) according to protocol for Gram-positive bacteria.

    Article Title: Production of Acylated Homoserine Lactones by Psychrotrophic Members of the Enterobacteriaceae Isolated from Foods
    Article Snippet: Strains of Enterobacteriaceae present in vacuum-packed and CO2 -packed cold-smoked salmon stored at 5°C were enumerated on Trypticase soy agar (TSA) (catalog no. CM131; Oxoid, Basingstoke, England) overlaid with double-strength violet red bile glucose agar (VRBG) (catalog no. CM485; Oxoid). .. The strains were isolated from VRBG plates and cultured on TSA plates ( ) and in Luria-Bertani (LB) broth, Miller (catalog no. 0446-17-3; Difco Laboratories, Detroit, Mich.).

    other:

    Article Title: Staphylococcus aureus Exploits a Non-ribosomal Cyclic Dipeptide to Modulate Survival within Epithelial Cells and Phagocytes
    Article Snippet: Staphylococcus aureus strains were routinely grown on Trypticase soy agar (TSA) or in Trypticase soy broth (TSB) supplemented with glucose (Oxoid).

    Polymerase Chain Reaction:

    Article Title: Postmenopause as a key factor in the composition of the Endometrial Cancer Microbiome (ECbiome)
    Article Snippet: They were subsequently plated on Trypticase soy agar (TSA) with 5% sheep blood (ThermoFisher Scientific, Waltham, MA) (following ATCC guidelines). .. We also produced cultures of Escherichia coli K12 Strain MSG 123 (ATCC PTA-7555), Staphylococcus aureus (ATCC 12600), and Lactobacillus iners (ATCC 55195) to test PCR probe specificity.

    Molecular Weight:

    Article Title: Development and Validation of an In Vitro Pharmacokinetic/Pharmacodynamic Model To Test the Antibacterial Efficacy of Antibiotic Polymer Conjugates
    Article Snippet: Colistin sulfate, ethanol, human salivary α-amylase, 4-dimethylaminopyridine (DMAP), anhydrous N , N -dimethylformamide (DMF), succinic anhydride, diethyl ether, type 1 dextrin from cornstarch (molecular weight, ∼8,100 g/mol), and carbonic anhydrase (molecular weight, 29,000 g/mol) were purchased from Sigma-Aldrich (Poole, United Kingdom). .. Trypticase soy agar (TSA), Trypticase soy broth (TSB), and cation-adjusted Mueller-Hinton broth (CAMHB, lot 43145) were purchased from Oxoid (Basingstoke, United Kingdom).

    DNA Extraction:

    Article Title: Genotypic and phenotypic characterization of biofilm production by Staphylococcus aureus strains isolated from bovine intramammary infections in Colombian dairy farms
    Article Snippet: .. 2.2 DNA extraction S. aureus strains stocks were thawed and cultured on Trypticase soy agar (TSA) (Oxoid, United Kingdom). .. DNA was extracted using DNeasy Blood & Tissue kit (Qiagen, Germany) according to protocol for Gram-positive bacteria.

    Mutagenesis:

    Article Title: Role of the Helicobacter pylori Sensor Kinase ArsS in Protein Trafficking and Acid Acclimation
    Article Snippet: A nonpolar ATCC 43504 Δ arsS deletion mutant was constructed by allelic exchange, as previously described ( ) and as summarized below. .. Bacteria were grown under microaerobic conditions (5% O2 , 10% CO2 , and 85% N2 ) either on Trypticase soy agar (TSA) plates supplemented with 5% sheep blood (Gibco) or in brain heart infusion (BHI) medium (Difco) supplemented with 7% horse serum (Gibco) and 0.25% yeast extract (Difco).

    Article Title: Phosphorylation dependent and independent regulation of Helicobacter pylori acid acclimation by the ArsRS two-component system
    Article Snippet: An arsR(D52N) mutant was constructed as described below. .. Bacteria were grown under microaerobic conditions (5% O2 , 10% CO2 , 85% N2 ) either on Trypticase Soy Agar (TSA) plates supplemented with 5% sheep blood (Gibco BRL-Life Technologies) or in brain heart infusion (BHI) medium (Difco Laboratories) supplemented with 7% horse serum (Gibco BRL-Life Technologies) and 0.25% yeast extract (Difco Laboratories).

    Isolation:

    Article Title: Production of Acylated Homoserine Lactones by Psychrotrophic Members of the Enterobacteriaceae Isolated from Foods
    Article Snippet: Strains of Enterobacteriaceae present in vacuum-packed and CO2 -packed cold-smoked salmon stored at 5°C were enumerated on Trypticase soy agar (TSA) (catalog no. CM131; Oxoid, Basingstoke, England) overlaid with double-strength violet red bile glucose agar (VRBG) (catalog no. CM485; Oxoid). .. The strains were isolated from VRBG plates and cultured on TSA plates ( ) and in Luria-Bertani (LB) broth, Miller (catalog no. 0446-17-3; Difco Laboratories, Detroit, Mich.).

    Mouse Assay:

    Article Title: Characterization of surface proteins of Cronobacter muytjensii using monoclonal antibodies and MALDI-TOF Mass spectrometry
    Article Snippet: Bacteria and growth conditions Stock cultures were maintained through out this study on Trypticase Soy Agar (TSA) (Oxoid, UK) or nutrient agar plates (HiMedia, India) at 4°C until use. .. Bacterial cells were heated at 80°C for 20 min in a water bath and were subsequently used for immunization of mice and screening of hybridoma cells for MAbs production using ELISA.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Phosphorylation dependent and independent regulation of Helicobacter pylori acid acclimation by the ArsRS two-component system
    Article Snippet: A non-polar ATCC 43504 ΔarsS deletion mutant was constructed by allelic exchange using a kanamycin resistance gene as described previously, and downstream gene expression in the knockout was confirmed by RT-PCR amplification of the downstream HP0163 gene ( ). .. Bacteria were grown under microaerobic conditions (5% O2 , 10% CO2 , 85% N2 ) either on Trypticase Soy Agar (TSA) plates supplemented with 5% sheep blood (Gibco BRL-Life Technologies) or in brain heart infusion (BHI) medium (Difco Laboratories) supplemented with 7% horse serum (Gibco BRL-Life Technologies) and 0.25% yeast extract (Difco Laboratories).

    Purification:

    Article Title: Novel Tellurite-Amended Media and Specific Chromosomal and Ti Plasmid Probes for Direct Analysis of Soil Populations of Agrobacterium Biovars 1 and 2
    Article Snippet: Total viable heterotrophic bacteria were counted on Trypticase soy agar (TSA) (Gibco BRL, Rockville, Md.) diluted 1/10. .. Agrobacterium -like colonies were purified by suspending individual colonies for at least 30 min in sterile distilled water and then streaking them on LPG agar.

    Knock-Out:

    Article Title: Phosphorylation dependent and independent regulation of Helicobacter pylori acid acclimation by the ArsRS two-component system
    Article Snippet: A non-polar ATCC 43504 ΔarsS deletion mutant was constructed by allelic exchange using a kanamycin resistance gene as described previously, and downstream gene expression in the knockout was confirmed by RT-PCR amplification of the downstream HP0163 gene ( ). .. Bacteria were grown under microaerobic conditions (5% O2 , 10% CO2 , 85% N2 ) either on Trypticase Soy Agar (TSA) plates supplemented with 5% sheep blood (Gibco BRL-Life Technologies) or in brain heart infusion (BHI) medium (Difco Laboratories) supplemented with 7% horse serum (Gibco BRL-Life Technologies) and 0.25% yeast extract (Difco Laboratories).

    Colony Assay:

    Article Title: Structure-function analysis of Avian β-defensin-6 and β-defensin-12: role of charge and disulfide bridges
    Article Snippet: All bacterial strains were grown and maintained on Trypticase Soy Agar (TSA) with 5 % Sheep Blood (Thermo Fisher Scientific) at 37 °C. .. The bactericidal activity of AvBD-6 and AvBD-12 was determined by colony counting assay [ ].

    Produced:

    Article Title: Postmenopause as a key factor in the composition of the Endometrial Cancer Microbiome (ECbiome)
    Article Snippet: They were subsequently plated on Trypticase soy agar (TSA) with 5% sheep blood (ThermoFisher Scientific, Waltham, MA) (following ATCC guidelines). .. We also produced cultures of Escherichia coli K12 Strain MSG 123 (ATCC PTA-7555), Staphylococcus aureus (ATCC 12600), and Lactobacillus iners (ATCC 55195) to test PCR probe specificity.

    Concentration Assay:

    Article Title: Structure-function analysis of Avian β-defensin-6 and β-defensin-12: role of charge and disulfide bridges
    Article Snippet: All bacterial strains were grown and maintained on Trypticase Soy Agar (TSA) with 5 % Sheep Blood (Thermo Fisher Scientific) at 37 °C. .. Ten microliters of bacterial suspension was inoculated into 5 ml 100× diluted Mueller-Hinton broth (MHB) to obtain a final concentration of approximately 2 × 105 CFU/ml.

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  • 99
    Thermo Fisher tsa blood agar
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    https://www.bioz.com/result/tsa blood agar/product/Thermo Fisher
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    Thermo Fisher trypticase soy agar tsa
    Trypticase Soy Agar Tsa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar tsa/product/Thermo Fisher
    Average 95 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar tsa - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    94
    Thermo Fisher trypticase soy agar
    Trypticase Soy Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar/product/Thermo Fisher
    Average 94 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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