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Difco trypticase soy agar slant
Trypticase Soy Agar Slant, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trypticase soy agar slant/product/Difco
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
trypticase soy agar slant - by Bioz Stars, 2020-04
86/100 stars

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Concentration Assay:

Article Title: Efficacies of Sodium Hypochlorite and Quaternary Ammonium Sanitizers for Reduction of Norovirus and Selected Bacteria during Ware-Washing Operations
Article Snippet: The stock culture of each organism was prepared by transferring a loopful of the E. coli and L. innocua into 50 ml of Trypticase soy broth (Difco, Becton Dickinson, Sparks, MD) containing 0.3% (wt/wt) yeast extract (Fisher Scientific) (TSBYE) and then incubation of the cultures at 37°C for 24 h. A loopful of this broth was then inoculated into a Trypticase soy agar slant (Difco, Becton Dickinson) supplemented with 0.3% (wt/wt) yeast extract (TSAYE) and incubated at 37°C for 24 h. This TSAYE containing the cell cultures was stored in a refrigerator at 4°C and used as a stock culture. .. The supernatant was decanted and the cell suspension was resuspended in 100 ml of 0.1 M potassium phosphate buffer (pH 7.2) until an initial concentration of approximately 1.0×109 CFU/ml for both E. coli K12 and L. innocua was achieved.

Incubation:

Article Title: Efficacies of Sodium Hypochlorite and Quaternary Ammonium Sanitizers for Reduction of Norovirus and Selected Bacteria during Ware-Washing Operations
Article Snippet: .. The stock culture of each organism was prepared by transferring a loopful of the E. coli and L. innocua into 50 ml of Trypticase soy broth (Difco, Becton Dickinson, Sparks, MD) containing 0.3% (wt/wt) yeast extract (Fisher Scientific) (TSBYE) and then incubation of the cultures at 37°C for 24 h. A loopful of this broth was then inoculated into a Trypticase soy agar slant (Difco, Becton Dickinson) supplemented with 0.3% (wt/wt) yeast extract (TSAYE) and incubated at 37°C for 24 h. This TSAYE containing the cell cultures was stored in a refrigerator at 4°C and used as a stock culture. .. Prior to each experiment, a loopful of either E. coli K12 or L. innocua stock culture was propagated aerobically in 100 ml TSBYE at 37°C for 24 h. Each cell broth was centrifuged (Kendro Laboratory Products, Sorvall RC 5C Plus, Newtown, CT) at 6,000 rpm for 10 min at 4°C.

Transferring:

Article Title: Efficacies of Sodium Hypochlorite and Quaternary Ammonium Sanitizers for Reduction of Norovirus and Selected Bacteria during Ware-Washing Operations
Article Snippet: .. The stock culture of each organism was prepared by transferring a loopful of the E. coli and L. innocua into 50 ml of Trypticase soy broth (Difco, Becton Dickinson, Sparks, MD) containing 0.3% (wt/wt) yeast extract (Fisher Scientific) (TSBYE) and then incubation of the cultures at 37°C for 24 h. A loopful of this broth was then inoculated into a Trypticase soy agar slant (Difco, Becton Dickinson) supplemented with 0.3% (wt/wt) yeast extract (TSAYE) and incubated at 37°C for 24 h. This TSAYE containing the cell cultures was stored in a refrigerator at 4°C and used as a stock culture. .. Prior to each experiment, a loopful of either E. coli K12 or L. innocua stock culture was propagated aerobically in 100 ml TSBYE at 37°C for 24 h. Each cell broth was centrifuged (Kendro Laboratory Products, Sorvall RC 5C Plus, Newtown, CT) at 6,000 rpm for 10 min at 4°C.

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  • 86
    Difco trypticase soy agar slant
    Trypticase Soy Agar Slant, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar slant/product/Difco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar slant - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    84
    Difco trypticase soy agar slants
    Trypticase Soy Agar Slants, supplied by Difco, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar slants/product/Difco
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar slants - by Bioz Stars, 2020-04
    84/100 stars
      Buy from Supplier

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