trypticase soy agar plates  (Thermo Fisher)


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    Structured Review

    Thermo Fisher trypticase soy agar plates
    Trypticase Soy Agar Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar plates/product/Thermo Fisher
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar plates - by Bioz Stars, 2020-04
    93/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Characterization of Helicobacter pylori cagA and vacA Genotypes among Alaskans and Their Correlation with Clinical Disease ▿
    Article Snippet: .. At the time of DNA extraction, stored H. pylori organisms were cultured on Trypticase soy agar plates containing 5% sheep blood (Remel, Lenexa, KS) and incubated for 3 to 5 days at 37°C in 12% CO2 and high humidity. ..

    Diagnostic Assay:

    Article Title: Interactions of organic acids with Campylobacter coli from swine
    Article Snippet: .. Several C . coli colonies were selected from the trypticase soy agar plates and diluted in 5 ml of Sensititre™ cation adjusted Mueller-Hinton broth w/TES (Remel Lenexa, KS, USA) to a 0.5 McFarland standard in a Nephelometer (TREK Diagnostic Systems Ltd., East Grinsted, UK). .. Since our experiments have a final total liquid volume of 100 μl in each well, to maintain a consistent bacterial concentration as suggested by the TREK Diagnostic Systems sensititre susceptibility test for Campylobacter , 200 μl of the 0.5 McFarland suspension was placed in tubes containing 11 ml of Sensititre™ cation adjusted Mueller-Hinton broth w/TES w/Lysed horse blood to provide 1 × 106 CFU/ml.

    Article Title: Interactions of organic acids with Campylobacter coli from swine
    Article Snippet: .. Several C . coli colonies were selected from the trypticase soy agar plates and diluted in 5 ml of Sensititre™ cation adjusted Mueller-Hinton broth w/TES (Remel Lenexa, KS, USA) to a 0.5 McFarland standard in a Nephelometer (TREK Diagnostic Systems Ltd., East Grinsted, UK). .. Since our experiments have a final total liquid volume of 100 μl in each well, to maintain a consistent bacterial concentration as suggested by the TREK Diagnostic Systems sensititre susceptibility test for Campylobacter , 200 μl of the 0.5 McFarland suspension was placed in tubes containing 11 ml of Sensititre™ cation adjusted Mueller-Hinton broth w/TES w/Lysed horse blood to provide 1 × 106 CFU/ml.

    Multiplex Assay:

    Article Title: Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus Strains from Louisiana Retail Meats ▿
    Article Snippet: Following 48 h of incubation, three to six presumptive S. aureus and MRSA colonies per meat sample (black colonies surrounded by 2- to 5-mm clear zones) were transferred to Trypticase soy agar plates followed by confirmation by a tube coagulase test (Remel, Lenexa, KS). .. Confirmation of S. aureus and MRSA was conducted using a multiplex PCR for the species-specific 442-bp fragment with unknown coding potential ( ) and mecA encoding a penicillin-binding protein with a low affinity for β-lactams ( ).

    Binding Assay:

    Article Title: Cable Pili and the 22-Kilodalton Adhesin Are Required for Burkholderia cenocepacia Binding to and Transmigration across the Squamous Epithelium
    Article Snippet: E. coli and B. cenocepacia cells were routinely grown on either Luria agar containing 1.5% Bacto agar (Difco, Franklin Lakes, NJ) or trypticase soy agar plates (Remel, Lenexa, KS). .. For the bacterial binding assay, B. cenocepacia cells were grown in trypticase soy broth in the absence or presence of appropriate antibiotics and [35 S]Cys-Met (Amersham Canada Ltd., Oakville, Ontario, Canada).

    Positive Control:

    Article Title: Interactions of organic acids with Campylobacter coli from swine
    Article Snippet: Several C . coli colonies were selected from the trypticase soy agar plates and diluted in 5 ml of Sensititre™ cation adjusted Mueller-Hinton broth w/TES (Remel Lenexa, KS, USA) to a 0.5 McFarland standard in a Nephelometer (TREK Diagnostic Systems Ltd., East Grinsted, UK). .. Briefly, the OA dilutions consisted of 50 μl of each OA solution placed in wells 1 and 2, and the well 2 solution was diluted 1:2 across a 96-well U-bottom Greiner bio-one microplate through column 11, and column 12 was used as the positive control [ ].

    Article Title: Interactions of organic acids with Campylobacter coli from swine
    Article Snippet: Several C . coli colonies were selected from the trypticase soy agar plates and diluted in 5 ml of Sensititre™ cation adjusted Mueller-Hinton broth w/TES (Remel Lenexa, KS, USA) to a 0.5 McFarland standard in a Nephelometer (TREK Diagnostic Systems Ltd., East Grinsted, UK). .. Briefly, the OA dilutions consisted of 50 μl of each OA solution placed in wells 1 and 2, and the well 2 solution was diluted 1:2 across a 96-well U-bottom Greiner bio-one microplate through column 11, and column 12 was used as the positive control [ ].

    Polymerase Chain Reaction:

    Article Title: Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus Strains from Louisiana Retail Meats ▿
    Article Snippet: Following 48 h of incubation, three to six presumptive S. aureus and MRSA colonies per meat sample (black colonies surrounded by 2- to 5-mm clear zones) were transferred to Trypticase soy agar plates followed by confirmation by a tube coagulase test (Remel, Lenexa, KS). .. Confirmation of S. aureus and MRSA was conducted using a multiplex PCR for the species-specific 442-bp fragment with unknown coding potential ( ) and mecA encoding a penicillin-binding protein with a low affinity for β-lactams ( ).

    Mutagenesis:

    Article Title: A Nonoligomerizing Mutant Form of Helicobacter pylori VacA Allows Structural Analysis of the p33 Domain
    Article Snippet: H. pylori strains were routinely grown on Trypticase soy agar plates containing 5% sheep blood or in sulfite-free brucella broth ( ) supplemented with cholesterol lipid concentrate (Gibco) (BB-cholesterol) ( ). .. Mutant strains were cultured initially on brucella agar plates containing 10% fetal bovine serum (FBS) supplemented with chloramphenicol (5 μg/ml) or metronidazole (3.75 μg/ml) and subsequently grown on medium without antibiotics.

    Isolation:

    Article Title: Contribution of Burkholderia cenocepacia Flagella to Infectivity and Inflammation
    Article Snippet: Escherichia coli and B. cenocepacia were routinely grown on either Luria agar (LA) containing 1.5% Bacto Agar (Difco, Franklin Lakes, N.J.) or Trypticase soy agar plates (Remel, Lenexa, Kans.). .. B. cenocepacia was also grown on Pseudomonas Isolation Agar (Difco) made in accordance with the manufacturer's instruction.

    Article Title: Porphyromonas gingivalis short fimbriae are regulated by a FimS/FimR two-component system
    Article Snippet: .. RNA isolation and qPCR Porphyromonas gingivalis strains were grown anaerobically on Trypticase soy agar plates at 37°C for 48 h. Porphyromonas gingivalis cells were collected and mixed in Trizol Reagent (Invitrogen). .. The RNA in the supernatant was then purified using an RNeasy mini spin column (Qiagen, Valencia, CA).

    Article Title: Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus Strains from Louisiana Retail Meats ▿
    Article Snippet: Paragraph title: Isolation and characterization of S. aureus and MRSA. ... Following 48 h of incubation, three to six presumptive S. aureus and MRSA colonies per meat sample (black colonies surrounded by 2- to 5-mm clear zones) were transferred to Trypticase soy agar plates followed by confirmation by a tube coagulase test (Remel, Lenexa, KS).

    Article Title: Cable Pili and the 22-Kilodalton Adhesin Are Required for Burkholderia cenocepacia Binding to and Transmigration across the Squamous Epithelium
    Article Snippet: E. coli and B. cenocepacia cells were routinely grown on either Luria agar containing 1.5% Bacto agar (Difco, Franklin Lakes, NJ) or trypticase soy agar plates (Remel, Lenexa, KS). .. B. cenocepacia was also grown on Pseudomonas isolation agar (Difco, Franklin Lakes, NJ) according to the manufacturer's instructions.

    Article Title: Characterization of Helicobacter pylori cagA and vacA Genotypes among Alaskans and Their Correlation with Clinical Disease ▿
    Article Snippet: At the time of DNA extraction, stored H. pylori organisms were cultured on Trypticase soy agar plates containing 5% sheep blood (Remel, Lenexa, KS) and incubated for 3 to 5 days at 37°C in 12% CO2 and high humidity. .. DNA was extracted with the MagNA Pure Compact using MagNA Pure Compact nucleic acid isolation kit I and the DNA Cultured Cells v3.1 protocol (Roche Applied Science, Indianapolis, IN).

    Article Title: Pneumococcal Serotypes Recovered from Health Children and Their Possible Association with Risk Factor in Istanbul, Turkey
    Article Snippet: Each swab was immediately inoculated on trypticase soy agar plates (Oxoid Ltd., Basingstoke, UK) supplemented with 5% defibrinated sheep’s blood. .. After incubation overnight at 37 °C in 5% CO2 atmosphere, characteristic α-haemolytic colonies were isolated and identified as S. pneumoniae using optochin susceptibility (5 U disk) and bile solubility tests.

    Solubility:

    Article Title: Pneumococcal Serotypes Recovered from Health Children and Their Possible Association with Risk Factor in Istanbul, Turkey
    Article Snippet: Each swab was immediately inoculated on trypticase soy agar plates (Oxoid Ltd., Basingstoke, UK) supplemented with 5% defibrinated sheep’s blood. .. After incubation overnight at 37 °C in 5% CO2 atmosphere, characteristic α-haemolytic colonies were isolated and identified as S. pneumoniae using optochin susceptibility (5 U disk) and bile solubility tests.

    Infection:

    Article Title: Relating the Physical Properties of Pseudomonas aeruginosa Lipopolysaccharides to Virulence by Atomic Force Microscopy ▿ Lipopolysaccharides to Virulence by Atomic Force Microscopy ▿ †
    Article Snippet: Paragraph title: Intranasal mouse infection studies. ... Bacterial strains were grown on Trypticase soy agar plates (Remel, Lenexa, KS) for 12 h at 37°C, resuspended in PBS to an OD650 of 0.5, and then diluted to obtain the desired dose in 20 μl.

    Mouse Assay:

    Article Title: Relating the Physical Properties of Pseudomonas aeruginosa Lipopolysaccharides to Virulence by Atomic Force Microscopy ▿ Lipopolysaccharides to Virulence by Atomic Force Microscopy ▿ †
    Article Snippet: Bacterial strains were grown on Trypticase soy agar plates (Remel, Lenexa, KS) for 12 h at 37°C, resuspended in PBS to an OD650 of 0.5, and then diluted to obtain the desired dose in 20 μl. .. Six- to 8-week-old female BALB/c mice (Harlan Laboratories) were anesthetized by intraperitoneal injection with 0.2 ml of ketamine (6.7 mg/ml) and xylazine (1.3 mg/ml) in 0.9% saline, and then 10 μl of the bacterial inoculum was placed into each nostril.

    Real-time Polymerase Chain Reaction:

    Article Title: Porphyromonas gingivalis short fimbriae are regulated by a FimS/FimR two-component system
    Article Snippet: .. RNA isolation and qPCR Porphyromonas gingivalis strains were grown anaerobically on Trypticase soy agar plates at 37°C for 48 h. Porphyromonas gingivalis cells were collected and mixed in Trizol Reagent (Invitrogen). .. The RNA in the supernatant was then purified using an RNeasy mini spin column (Qiagen, Valencia, CA).

    Concentration Assay:

    Article Title: Interactions of organic acids with Campylobacter coli from swine
    Article Snippet: Several C . coli colonies were selected from the trypticase soy agar plates and diluted in 5 ml of Sensititre™ cation adjusted Mueller-Hinton broth w/TES (Remel Lenexa, KS, USA) to a 0.5 McFarland standard in a Nephelometer (TREK Diagnostic Systems Ltd., East Grinsted, UK). .. Since our experiments have a final total liquid volume of 100 μl in each well, to maintain a consistent bacterial concentration as suggested by the TREK Diagnostic Systems sensititre susceptibility test for Campylobacter , 200 μl of the 0.5 McFarland suspension was placed in tubes containing 11 ml of Sensititre™ cation adjusted Mueller-Hinton broth w/TES w/Lysed horse blood to provide 1 × 106 CFU/ml.

    Article Title: Interactions of organic acids with Campylobacter coli from swine
    Article Snippet: Several C . coli colonies were selected from the trypticase soy agar plates and diluted in 5 ml of Sensititre™ cation adjusted Mueller-Hinton broth w/TES (Remel Lenexa, KS, USA) to a 0.5 McFarland standard in a Nephelometer (TREK Diagnostic Systems Ltd., East Grinsted, UK). .. Since our experiments have a final total liquid volume of 100 μl in each well, to maintain a consistent bacterial concentration as suggested by the TREK Diagnostic Systems sensititre susceptibility test for Campylobacter , 200 μl of the 0.5 McFarland suspension was placed in tubes containing 11 ml of Sensititre™ cation adjusted Mueller-Hinton broth w/TES w/Lysed horse blood to provide 1 × 106 CFU/ml.

    Incubation:

    Article Title: Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus Strains from Louisiana Retail Meats ▿
    Article Snippet: .. Following 48 h of incubation, three to six presumptive S. aureus and MRSA colonies per meat sample (black colonies surrounded by 2- to 5-mm clear zones) were transferred to Trypticase soy agar plates followed by confirmation by a tube coagulase test (Remel, Lenexa, KS). .. Confirmation of S. aureus and MRSA was conducted using a multiplex PCR for the species-specific 442-bp fragment with unknown coding potential ( ) and mecA encoding a penicillin-binding protein with a low affinity for β-lactams ( ).

    Article Title: Characterization of Helicobacter pylori cagA and vacA Genotypes among Alaskans and Their Correlation with Clinical Disease ▿
    Article Snippet: .. At the time of DNA extraction, stored H. pylori organisms were cultured on Trypticase soy agar plates containing 5% sheep blood (Remel, Lenexa, KS) and incubated for 3 to 5 days at 37°C in 12% CO2 and high humidity. ..

    Article Title: Interactions of organic acids with Campylobacter coli from swine
    Article Snippet: All Campylobacter susceptibility studies required incubation for 48 hours at 42°C either on trypticase soy agar w/5% sheep blood or in 96-well plates (U-bottom microplate, Greiner bio-one North America Inc., Monroe, North Carolina, USA) for broth microdilution testing because there were some strains that did not grow a sufficient amount in 24 hours to run the test. .. Several C . coli colonies were selected from the trypticase soy agar plates and diluted in 5 ml of Sensititre™ cation adjusted Mueller-Hinton broth w/TES (Remel Lenexa, KS, USA) to a 0.5 McFarland standard in a Nephelometer (TREK Diagnostic Systems Ltd., East Grinsted, UK).

    Article Title: Interactions of organic acids with Campylobacter coli from swine
    Article Snippet: All Campylobacter susceptibility studies required incubation for 48 hours at 42°C either on trypticase soy agar w/5% sheep blood or in 96-well plates (U-bottom microplate, Greiner bio-one North America Inc., Monroe, North Carolina, USA) for broth microdilution testing because there were some strains that did not grow a sufficient amount in 24 hours to run the test. .. Several C . coli colonies were selected from the trypticase soy agar plates and diluted in 5 ml of Sensititre™ cation adjusted Mueller-Hinton broth w/TES (Remel Lenexa, KS, USA) to a 0.5 McFarland standard in a Nephelometer (TREK Diagnostic Systems Ltd., East Grinsted, UK).

    Article Title: Pneumococcal Serotypes Recovered from Health Children and Their Possible Association with Risk Factor in Istanbul, Turkey
    Article Snippet: Each swab was immediately inoculated on trypticase soy agar plates (Oxoid Ltd., Basingstoke, UK) supplemented with 5% defibrinated sheep’s blood. .. After incubation overnight at 37 °C in 5% CO2 atmosphere, characteristic α-haemolytic colonies were isolated and identified as S. pneumoniae using optochin susceptibility (5 U disk) and bile solubility tests.

    other:

    Article Title: Adherent-invasive Escherichia coli (AIEC) in pediatric Crohn’s disease patients: phenotypic and genetic pathogenic features
    Article Snippet: Strains were usually grown in Brain Heart Infusion (BHI, Oxoid, Rome, Italy) or on Trypticase Soy Agar plates (TSA, Oxoid) overnight at 37°C.

    Article Title: Phagocyte NADPH Oxidase, but Not Inducible Nitric Oxide Synthase, Is Essential for Early Control of Burkholderia cepacia and Chromobacterium violaceum Infection in Mice
    Article Snippet: Trypticase soy agar plates were purchased from Remel, Inc. (Lenexa, Kans.).

    Cell Culture:

    Article Title: A Nonoligomerizing Mutant Form of Helicobacter pylori VacA Allows Structural Analysis of the p33 Domain
    Article Snippet: H. pylori strains were routinely grown on Trypticase soy agar plates containing 5% sheep blood or in sulfite-free brucella broth ( ) supplemented with cholesterol lipid concentrate (Gibco) (BB-cholesterol) ( ). .. Mutant strains were cultured initially on brucella agar plates containing 10% fetal bovine serum (FBS) supplemented with chloramphenicol (5 μg/ml) or metronidazole (3.75 μg/ml) and subsequently grown on medium without antibiotics.

    Article Title: Characterization of Helicobacter pylori cagA and vacA Genotypes among Alaskans and Their Correlation with Clinical Disease ▿
    Article Snippet: .. At the time of DNA extraction, stored H. pylori organisms were cultured on Trypticase soy agar plates containing 5% sheep blood (Remel, Lenexa, KS) and incubated for 3 to 5 days at 37°C in 12% CO2 and high humidity. ..

    Sequencing:

    Article Title: Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus Strains from Louisiana Retail Meats ▿
    Article Snippet: Following 48 h of incubation, three to six presumptive S. aureus and MRSA colonies per meat sample (black colonies surrounded by 2- to 5-mm clear zones) were transferred to Trypticase soy agar plates followed by confirmation by a tube coagulase test (Remel, Lenexa, KS). .. Multilocus sequence typing (MLST) ( ) and single-locus DNA sequencing of the repeat region of the Staphylococcus protein A gene ( spa ) ( ) were performed for three representative MRSA isolates from each MRSA-positive store.

    Injection:

    Article Title: Relating the Physical Properties of Pseudomonas aeruginosa Lipopolysaccharides to Virulence by Atomic Force Microscopy ▿ Lipopolysaccharides to Virulence by Atomic Force Microscopy ▿ †
    Article Snippet: Bacterial strains were grown on Trypticase soy agar plates (Remel, Lenexa, KS) for 12 h at 37°C, resuspended in PBS to an OD650 of 0.5, and then diluted to obtain the desired dose in 20 μl. .. Six- to 8-week-old female BALB/c mice (Harlan Laboratories) were anesthetized by intraperitoneal injection with 0.2 ml of ketamine (6.7 mg/ml) and xylazine (1.3 mg/ml) in 0.9% saline, and then 10 μl of the bacterial inoculum was placed into each nostril.

    Purification:

    Article Title: Porphyromonas gingivalis short fimbriae are regulated by a FimS/FimR two-component system
    Article Snippet: RNA isolation and qPCR Porphyromonas gingivalis strains were grown anaerobically on Trypticase soy agar plates at 37°C for 48 h. Porphyromonas gingivalis cells were collected and mixed in Trizol Reagent (Invitrogen). .. The RNA in the supernatant was then purified using an RNeasy mini spin column (Qiagen, Valencia, CA).

    DNA Sequencing:

    Article Title: Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus Strains from Louisiana Retail Meats ▿
    Article Snippet: Following 48 h of incubation, three to six presumptive S. aureus and MRSA colonies per meat sample (black colonies surrounded by 2- to 5-mm clear zones) were transferred to Trypticase soy agar plates followed by confirmation by a tube coagulase test (Remel, Lenexa, KS). .. Multilocus sequence typing (MLST) ( ) and single-locus DNA sequencing of the repeat region of the Staphylococcus protein A gene ( spa ) ( ) were performed for three representative MRSA isolates from each MRSA-positive store.

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