tryptic soy broth agar tsa  (Millipore)


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  • 99
    Name:
    Tryptic Soy Broth
    Description:

    Catalog Number:
    t8907
    Price:
    None
    Applications:
    General purpose powdered broth medium for the cultivation of fastidious microorganisms.
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    Structured Review

    Millipore tryptic soy broth agar tsa

    https://www.bioz.com/result/tryptic soy broth agar tsa/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tryptic soy broth agar tsa - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Localization and nucleotide specificity of Blastocystis succinyl-CoA synthetase
    Article Snippet: LYSGM is a modification of TYSGM-9 in which the trypticase and yeast extract of the latter are replaced with 0.25% yeast extract (Sigma) and 0.05% neutralized liver digest (Oxoid). .. Escherichia coli strain α select silver efficiency (Bioline) was used for cloning and BL21(DE3) pLysS (Bioline) was used for protein expression.

    Immunohistochemistry:

    Article Title: Interferon Regulatory Factor-1 in Flagellin-Induced Reprogramming: Potential Protective Role of CXCL10 in Cornea Innate Defense Against Pseudomonas aeruginosa Infection
    Article Snippet: To that end, we had to use PAO1 with 10-fold increase in CFU (105 ) to challenge B6 mouse corneas and assess host responses at 6 hours by isolating ECs for PCR analysis or immunohistochemistry. .. For direct bacterial challenge of HCECs, P. aeruginosa was grown in tryptic soy broth (Sigma-Aldrich) at 37°C until absorbance at 600 nm reached optical density value 0.5.

    Selection:

    Article Title: Outer Membrane Lipoprotein Lip40 Modulates Adherence, Colonization, and Virulence of Actinobacillus pleuropneumoniae
    Article Snippet: A. pleuropneumoniae strains were cultured on trypic soy agar (TSA; Dickinson and Company, Franklin Lakes, NJ, United States) or in tryptic soy broth (TSB), supplemented with 10 μg/ml nicotinamide adenine dinucleotide (NAD+ ; Sigma, St. Louis, MO, United States) and 5% fetal calf serum (Gibco BRL, Grand Island, NY, United States). .. During the construction of A. pleuropneumoniae lip40 mutants, 5 μg/ml chloramphenicol was added for the selection of single crossover mutants, 5% sucrose (m/v) was supplemented for double crossover mutant selection, and 2 μg/ml chloramphenicol was used for complementation strain selection.

    In Vitro:

    Article Title: Interferon Regulatory Factor-1 in Flagellin-Induced Reprogramming: Potential Protective Role of CXCL10 in Cornea Innate Defense Against Pseudomonas aeruginosa Infection
    Article Snippet: ATCC 19660 usually causes EC death in vitro and epithelial sheet loss in vivo. .. For direct bacterial challenge of HCECs, P. aeruginosa was grown in tryptic soy broth (Sigma-Aldrich) at 37°C until absorbance at 600 nm reached optical density value 0.5.

    Modification:

    Article Title: Localization and nucleotide specificity of Blastocystis succinyl-CoA synthetase
    Article Snippet: .. LYSGM is a modification of TYSGM-9 in which the trypticase and yeast extract of the latter are replaced with 0.25% yeast extract (Sigma) and 0.05% neutralized liver digest (Oxoid). ..

    Article Title: Targeting microbial biofilms using Ficin, a nonspecific plant protease
    Article Snippet: .. The Müller-Hinton broth (Fluka) or Trypticase soy broth (Sigma) did not provide stable biofilm formation by both Staphylococcus aureus and Staphylococcus epidermidis , as is has been determined in preliminary studies , thus the modified Basal medium (BM) (glucose 5 g, peptone 7 g, MgSO4 × 7H2 O 2.0 g and CaCl2 × 2H2 O 0.05 g in 1.0 liter tap water) was used for the biofilm formation assays . ..

    Article Title: New Derivatives of Pyridoxine Exhibit High Antibacterial Activity against Biofilm-Embedded Staphylococcus Cells
    Article Snippet: .. The Mueller-Hinton broth (Fluka), Trypticase soy broth (Sigma), Luria-Bertani broth (LB) (Trytone 10.0 g, yeast extract 5.0 g, and NaCl 5.0 g in 1.0 liter tap water), and modified Basal medium (BM) (glucose 5.0 g, peptone 7.0 g, MgSO4 × 7H2 O 2.0 g, and CaCl2 0.05 g in 1.0 liter tap water) were used as indicated. ..

    Mutagenesis:

    Article Title: Outer Membrane Lipoprotein Lip40 Modulates Adherence, Colonization, and Virulence of Actinobacillus pleuropneumoniae
    Article Snippet: A. pleuropneumoniae strains were cultured on trypic soy agar (TSA; Dickinson and Company, Franklin Lakes, NJ, United States) or in tryptic soy broth (TSB), supplemented with 10 μg/ml nicotinamide adenine dinucleotide (NAD+ ; Sigma, St. Louis, MO, United States) and 5% fetal calf serum (Gibco BRL, Grand Island, NY, United States). .. During the construction of A. pleuropneumoniae lip40 mutants, 5 μg/ml chloramphenicol was added for the selection of single crossover mutants, 5% sucrose (m/v) was supplemented for double crossover mutant selection, and 2 μg/ml chloramphenicol was used for complementation strain selection.

    Isolation:

    Article Title: Control of Relative Air Humidity as a Potential Means to Improve Hygiene on Surfaces: A Preliminary Approach with Listeria monocytogenes
    Article Snippet: Listeria monocytogenes EGDe strain (BUG1600) (serotype 1/2a) isolated from a case of animal listeriosis [ , ], CCL500 strain (serotype 1/2b) isolated from milk, CCL128 strain (serotype 4b) isolated from and persistent in the cheese industry, and LO28 strain (serotype 1/2c) isolated from a case of human listeriosis [ ], were used in this study. .. Strains were stored on tryptic soy broth supplemented with 20% glycerol (TSB; Sigma-Aldrich, St. Quentin Fallavier, France) at –80°C.

    In Vivo:

    Article Title: Interferon Regulatory Factor-1 in Flagellin-Induced Reprogramming: Potential Protective Role of CXCL10 in Cornea Innate Defense Against Pseudomonas aeruginosa Infection
    Article Snippet: ATCC 19660 usually causes EC death in vitro and epithelial sheet loss in vivo. .. For direct bacterial challenge of HCECs, P. aeruginosa was grown in tryptic soy broth (Sigma-Aldrich) at 37°C until absorbance at 600 nm reached optical density value 0.5.

    Cell Culture:

    Article Title: Effective delivery of volatile biocides employing mesoporous silicates for treating biofilms
    Article Snippet: Confocal laser scanning microscopy (CLSM) was used to visualize Pseudomonas aeruginosa biofilms grown in sterile FluoroDish™ tissue and cell culture dishes (35 mm diameter) with cover glass bottom (23 mm diameter; World Precision Instruments, UK). .. Control biofilms were grown for 48 h at 30°C in 2 ml sterile 10% tryptic soy broth (TSB) (Sigma-Aldrich) in PBS with 1/100 (vol/vol) P. aeruginosa inocula (OD600 = 0.132).

    Article Title: A Combined Enrichment and Aptamer Pulldown Assay for Francisella tularensis Detection in Food and Environmental Matrices
    Article Snippet: F. tularensis was subcultured in tryptic soy broth (TSB) supplemented with 0.1% L-cysteine (Sigma-Aldrich, St. Louis, MO) at 37°C with 120 rpm shaking. .. Environmental bacteria (herein referred to as soil bacteria) were obtained from one gram (g) of soil, hay and dust and cultured in TSB and diluted to 10−1 in TSB containing 0.1% MgSO4 .

    Article Title: Surface proteins that promote adherence of Staphylococcus aureus to human desquamated nasal epithelial cells
    Article Snippet: S. aureus strains were grown on tryptic soy agar (TSA; Oxoid), tryptic soy broth (TSB) or RPMI 1640 (Sigma). .. L. lactis strains were cultured in M17 medium (Difco) containing 0.5% (v/v) glucose without shaking at 30°C.

    Article Title: Outer Membrane Lipoprotein Lip40 Modulates Adherence, Colonization, and Virulence of Actinobacillus pleuropneumoniae
    Article Snippet: .. A. pleuropneumoniae strains were cultured on trypic soy agar (TSA; Dickinson and Company, Franklin Lakes, NJ, United States) or in tryptic soy broth (TSB), supplemented with 10 μg/ml nicotinamide adenine dinucleotide (NAD+ ; Sigma, St. Louis, MO, United States) and 5% fetal calf serum (Gibco BRL, Grand Island, NY, United States). .. During the construction of A. pleuropneumoniae lip40 mutants, 5 μg/ml chloramphenicol was added for the selection of single crossover mutants, 5% sucrose (m/v) was supplemented for double crossover mutant selection, and 2 μg/ml chloramphenicol was used for complementation strain selection.

    Concentration Assay:

    Article Title: Commonly Used Disinfectants Fail To Eradicate Salmonella enterica Biofilms from Food Contact Surface Materials
    Article Snippet: One milliliter of the 0.5 McFarland standard suspension was diluted into 9 ml of tryptic soy broth (TSB; Sigma-Aldrich) and vortexed. .. The stock concentrations of sodium hypochlorite (2,000 mg/liter), sodium hydroxide (4 M concentration), and benzalkonium chloride (0.08%) (Sigma-Aldrich) disinfectants were made before each test.

    Article Title: Plant Drought Tolerance Enhancement by Trehalose Production of Desiccation-Tolerant Microorganisms
    Article Snippet: Bacteria were grown in tryptic soy broth (TSB) or M9 Minimal Medium (Sigma M6030) with glucose or fructose (50 mM) as the sole carbon source at 30°C ( ). .. To prepare hypersaline minimal medium (HMM), we added NaCl (6 M) to M9 medium at the concentration specified by .

    Incubation:

    Article Title: Commonly Used Disinfectants Fail To Eradicate Salmonella enterica Biofilms from Food Contact Surface Materials
    Article Snippet: .. To establish biofilm, 1 ml of the suspension was diluted into 9 ml of tryptic soy broth (Sigma-Aldrich) and incubated at 37°C for 24 h in a shaking incubator. ..

    Article Title: Control of Relative Air Humidity as a Potential Means to Improve Hygiene on Surfaces: A Preliminary Approach with Listeria monocytogenes
    Article Snippet: Strains were stored on tryptic soy broth supplemented with 20% glycerol (TSB; Sigma-Aldrich, St. Quentin Fallavier, France) at –80°C. .. For revitalization, bacteria were isolated with a sterile swab on tryptic soy agar (TSA, Sigma-Aldrich) plate, incubated at 25°C for 48 h and stored at 4°C for a maximal period of one month.

    Confocal Laser Scanning Microscopy:

    Article Title: Effective delivery of volatile biocides employing mesoporous silicates for treating biofilms
    Article Snippet: Confocal laser scanning microscopy (CLSM) was used to visualize Pseudomonas aeruginosa biofilms grown in sterile FluoroDish™ tissue and cell culture dishes (35 mm diameter) with cover glass bottom (23 mm diameter; World Precision Instruments, UK). .. Control biofilms were grown for 48 h at 30°C in 2 ml sterile 10% tryptic soy broth (TSB) (Sigma-Aldrich) in PBS with 1/100 (vol/vol) P. aeruginosa inocula (OD600 = 0.132).

    Activity Assay:

    Article Title: Commonly Used Disinfectants Fail To Eradicate Salmonella enterica Biofilms from Food Contact Surface Materials
    Article Snippet: Paragraph title: Activity of disinfectants against planktonic cells. ... One milliliter of the 0.5 McFarland standard suspension was diluted into 9 ml of tryptic soy broth (TSB; Sigma-Aldrich) and vortexed.

    Imaging:

    Article Title: Effective delivery of volatile biocides employing mesoporous silicates for treating biofilms
    Article Snippet: Paragraph title: 2.6. Confocal imaging of bacterial biofilms ... Control biofilms were grown for 48 h at 30°C in 2 ml sterile 10% tryptic soy broth (TSB) (Sigma-Aldrich) in PBS with 1/100 (vol/vol) P. aeruginosa inocula (OD600 = 0.132).

    Expressing:

    Article Title: Localization and nucleotide specificity of Blastocystis succinyl-CoA synthetase
    Article Snippet: LYSGM is a modification of TYSGM-9 in which the trypticase and yeast extract of the latter are replaced with 0.25% yeast extract (Sigma) and 0.05% neutralized liver digest (Oxoid). .. Escherichia coli strain α select silver efficiency (Bioline) was used for cloning and BL21(DE3) pLysS (Bioline) was used for protein expression.

    Polymerase Chain Reaction:

    Article Title: Interferon Regulatory Factor-1 in Flagellin-Induced Reprogramming: Potential Protective Role of CXCL10 in Cornea Innate Defense Against Pseudomonas aeruginosa Infection
    Article Snippet: To that end, we had to use PAO1 with 10-fold increase in CFU (105 ) to challenge B6 mouse corneas and assess host responses at 6 hours by isolating ECs for PCR analysis or immunohistochemistry. .. For direct bacterial challenge of HCECs, P. aeruginosa was grown in tryptic soy broth (Sigma-Aldrich) at 37°C until absorbance at 600 nm reached optical density value 0.5.

    Transformation Assay:

    Article Title: Outer Membrane Lipoprotein Lip40 Modulates Adherence, Colonization, and Virulence of Actinobacillus pleuropneumoniae
    Article Snippet: A. pleuropneumoniae strains were cultured on trypic soy agar (TSA; Dickinson and Company, Franklin Lakes, NJ, United States) or in tryptic soy broth (TSB), supplemented with 10 μg/ml nicotinamide adenine dinucleotide (NAD+ ; Sigma, St. Louis, MO, United States) and 5% fetal calf serum (Gibco BRL, Grand Island, NY, United States). .. Escherichia coli β2155 transformed with transconjugation plasmid was grown on Luria–Bertani (LB) agar (Oxoid, Basingstoke, Hants, United Kingdom) or in LB broth with 50 μg/ml diaminopimelic acid (Sigma) and ampicillin (100 μg/ml).

    Plasmid Preparation:

    Article Title: Outer Membrane Lipoprotein Lip40 Modulates Adherence, Colonization, and Virulence of Actinobacillus pleuropneumoniae
    Article Snippet: A. pleuropneumoniae strains were cultured on trypic soy agar (TSA; Dickinson and Company, Franklin Lakes, NJ, United States) or in tryptic soy broth (TSB), supplemented with 10 μg/ml nicotinamide adenine dinucleotide (NAD+ ; Sigma, St. Louis, MO, United States) and 5% fetal calf serum (Gibco BRL, Grand Island, NY, United States). .. Escherichia coli β2155 transformed with transconjugation plasmid was grown on Luria–Bertani (LB) agar (Oxoid, Basingstoke, Hants, United Kingdom) or in LB broth with 50 μg/ml diaminopimelic acid (Sigma) and ampicillin (100 μg/ml).

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  • 93
    Millipore trypticase soy agar tsa
    Trypticase Soy Agar Tsa, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar tsa/product/Millipore
    Average 93 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar tsa - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

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