trypsinedta  (Thermo Fisher)


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  • 99
    Name:
    Trypsin EDTA Solution TE
    Description:
    Gibco Trypsin solutions are made from trypsin powder a mixture of proteases derived from bovine pancreas Due to its digestive strength it is widely used for cell dissociation during routine cell culture passaging and primary tissue dissociation This Gibco Trypsin EDTA solution is formulation as 0 025 trypsin and 0 01 EDTA in Phosphate Buffered Saline PBS We offer a variety of Gibco Trypsin and animal origin free TrypLE cell dissociation products to meet your needs This trypsin is manufactured as follows WithEDTAPhenol RedThe complete formulation is available Product UseFor Research Use Only Not for use in diagnostic procedures cGMP Manufacturing and Quality SystemGibco Trypsin is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards
    Catalog Number:
    r001100
    Price:
    None
    Applications:
    Cell Culture|Corneal Epithelial Cell Culture|Fibroblast Culture|Keratinocyte Culture|Large Vessel Endothelial Cell Culture|Mammalian Cell Culture|Mammary Epithelial Cell Culture|Melanocyte Culture|Microvascular Endothelial Cell Culture|Smooth Muscle Cell Culture|Primary Cell Culture
    Category:
    Cell Culture Transfection Reagents
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    Structured Review

    Thermo Fisher trypsinedta
    Gibco Trypsin solutions are made from trypsin powder a mixture of proteases derived from bovine pancreas Due to its digestive strength it is widely used for cell dissociation during routine cell culture passaging and primary tissue dissociation This Gibco Trypsin EDTA solution is formulation as 0 025 trypsin and 0 01 EDTA in Phosphate Buffered Saline PBS We offer a variety of Gibco Trypsin and animal origin free TrypLE cell dissociation products to meet your needs This trypsin is manufactured as follows WithEDTAPhenol RedThe complete formulation is available Product UseFor Research Use Only Not for use in diagnostic procedures cGMP Manufacturing and Quality SystemGibco Trypsin is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards
    https://www.bioz.com/result/trypsinedta/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypsinedta - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Modulation of liver regeneration via myeloid PTEN deficiency
    Article Snippet: .. After another 48 h, AML-12 cells were harvested with Trypsin-EDTA (Thermo Fisher Scientific) and counted with a FACSVerse flow cytometer (BD Biosciences). .. Western blot analysis Frozen liver samples were homogenized with RIPA buffer (Beyotime Biotechnology, Beijing, China) supplemented with PMSF (Beyotime Biotechnology) and PhosSTOP (Roche, Basel, Switzerland).

    Article Title: Sevoflurane protects rat brain endothelial barrier structure and function after hypoxia-reoxygenation injury
    Article Snippet: .. Flow cytometry and cell size measurement For flow and imaging cytometry cells were washed twice with 2.5mmol EDTA in PBS, detached with trypsin-EDTA solution (0.05%) (Thermo, Reinach, Switzerland) and put through a 35μm cell strainer (Falcon, Corning, USA) after resuspension. .. The fixable viability dye eFluor® 660 (e Bioscience, Hatfield, UK) was used for viability analysis in accordance with the manufacturer’s instructions.

    Article Title: Morphological and Functional Alterations of Alveolar Macrophages in a Murine Model of Chronic Inflammatory Lung Disease
    Article Snippet: .. To verify effective purification, adherent cells were detached by treatment with 0.05 % Trypsin/EDTA (Gibco), stained with antibodies against F4-80 and CD11c and analyzed by flow cytometry. .. For RNA analysis, adherent cells were directly lysed on the plastic surface by treatment with RLT buffer (Qiagen, supplemented with 1 % mercaptoethanol), and RNA extraction protocol was applied.

    Cytometry:

    Article Title: Modulation of liver regeneration via myeloid PTEN deficiency
    Article Snippet: .. After another 48 h, AML-12 cells were harvested with Trypsin-EDTA (Thermo Fisher Scientific) and counted with a FACSVerse flow cytometer (BD Biosciences). .. Western blot analysis Frozen liver samples were homogenized with RIPA buffer (Beyotime Biotechnology, Beijing, China) supplemented with PMSF (Beyotime Biotechnology) and PhosSTOP (Roche, Basel, Switzerland).

    Article Title: Sevoflurane protects rat brain endothelial barrier structure and function after hypoxia-reoxygenation injury
    Article Snippet: .. Flow cytometry and cell size measurement For flow and imaging cytometry cells were washed twice with 2.5mmol EDTA in PBS, detached with trypsin-EDTA solution (0.05%) (Thermo, Reinach, Switzerland) and put through a 35μm cell strainer (Falcon, Corning, USA) after resuspension. .. The fixable viability dye eFluor® 660 (e Bioscience, Hatfield, UK) was used for viability analysis in accordance with the manufacturer’s instructions.

    Article Title: Morphological and Functional Alterations of Alveolar Macrophages in a Murine Model of Chronic Inflammatory Lung Disease
    Article Snippet: .. To verify effective purification, adherent cells were detached by treatment with 0.05 % Trypsin/EDTA (Gibco), stained with antibodies against F4-80 and CD11c and analyzed by flow cytometry. .. For RNA analysis, adherent cells were directly lysed on the plastic surface by treatment with RLT buffer (Qiagen, supplemented with 1 % mercaptoethanol), and RNA extraction protocol was applied.

    Cell Culture:

    Article Title: Plasminogen activator inhibitor-1 detaches cells from extracellular matrices by inactivating integrins
    Article Snippet: .. FBS was from HyClone, RPMI and DME cell culture media and trypsin/EDTA cell dissociation solution were from Invitrogen, and nonenzymatic cell dissociation solution and CHAPS were from Sigma-Aldrich. ..

    Purification:

    Article Title: Morphological and Functional Alterations of Alveolar Macrophages in a Murine Model of Chronic Inflammatory Lung Disease
    Article Snippet: .. To verify effective purification, adherent cells were detached by treatment with 0.05 % Trypsin/EDTA (Gibco), stained with antibodies against F4-80 and CD11c and analyzed by flow cytometry. .. For RNA analysis, adherent cells were directly lysed on the plastic surface by treatment with RLT buffer (Qiagen, supplemented with 1 % mercaptoethanol), and RNA extraction protocol was applied.

    Incubation:

    Article Title: Novel mechanism regulating endothelial permeability via T-cadherin-dependent VE-cadherin phosphorylation and clathrin-mediated endocytosis
    Article Snippet: .. Trypsinization experiments To distinguish between intracellular and cell surface pools of VE-cadherin, HUVEC were rinsed in HBSS and incubated in 0.25 % trypsin/EDTA (GIBCO) at 37 °C to proteolytically remove cell surface VE-cadherin [ ]. ..

    other:

    Article Title: Preservation of epithelial progenitor cells from collagenase-digested oral mucosa during ex vivo cultivation
    Article Snippet: Materials DMEM:F12 (1:1), Opti-MEM, EpiLife medium, , trypsin-EDTA, phosphate-buffered saline (PBS), gentamicin, amphotericin B, Lipofectamine 2000 and Alexa-Fluor-conjugated secondary IgG were purchased from Invitrogen (Carlsbad, CA).

    Article Title: Quercetin-induced miR-200b-3p regulates the mode of self-renewing divisions in pancreatic cancer
    Article Snippet: Next, we evaluated the influence on proliferation by dissociating the tumorspheres from each group to single cells using trypsin-EDTA.

    Imaging:

    Article Title: Sevoflurane protects rat brain endothelial barrier structure and function after hypoxia-reoxygenation injury
    Article Snippet: .. Flow cytometry and cell size measurement For flow and imaging cytometry cells were washed twice with 2.5mmol EDTA in PBS, detached with trypsin-EDTA solution (0.05%) (Thermo, Reinach, Switzerland) and put through a 35μm cell strainer (Falcon, Corning, USA) after resuspension. .. The fixable viability dye eFluor® 660 (e Bioscience, Hatfield, UK) was used for viability analysis in accordance with the manufacturer’s instructions.

    Staining:

    Article Title: Human Osteoclasts are Inducible Immunosuppressive Cells in Response to T cell-Derived IFN-γ and CD40 Ligand in vitro
    Article Snippet: .. To detect the viability of OCs during the coculture with α-CD3/CD28 DynaBeads-stimulated T cells in Transwells, coculutred OCs were digested from the culture plate with Trypsin/EDTA, and stained by using LIVE/DEAD® Fixable Dead Cell Stain Kit (Molecular Probes, Life Technologies). .. Cells were measured by flow cytometry at UV450.

    Article Title: Morphological and Functional Alterations of Alveolar Macrophages in a Murine Model of Chronic Inflammatory Lung Disease
    Article Snippet: .. To verify effective purification, adherent cells were detached by treatment with 0.05 % Trypsin/EDTA (Gibco), stained with antibodies against F4-80 and CD11c and analyzed by flow cytometry. .. For RNA analysis, adherent cells were directly lysed on the plastic surface by treatment with RLT buffer (Qiagen, supplemented with 1 % mercaptoethanol), and RNA extraction protocol was applied.

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  • 99
    Thermo Fisher trypsinedta solution
    Trypsinedta Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsinedta solution/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypsinedta solution - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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