trypsin versene mixture  (Lonza)


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    Name:
    Versene
    Description:
    Versene EDTA 0 02 0 2 g L Ethylenediaminetetraacetic acid 0 53mM in DBPS without calcium or magnesium 100 mL
    Catalog Number:
    BE17-711E
    Price:
    None
    Category:
    Culture Media and Reagents
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    Structured Review

    Lonza trypsin versene mixture
    Versene EDTA 0 02 0 2 g L Ethylenediaminetetraacetic acid 0 53mM in DBPS without calcium or magnesium 100 mL
    https://www.bioz.com/result/trypsin versene mixture/product/Lonza
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypsin versene mixture - by Bioz Stars, 2021-05
    86/100 stars

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    Related Articles

    Electroporation:

    Article Title: Membrane flow drives an adhesion-independent amoeboid cell migration mode
    Article Snippet: .. For experiments performed in Ficoll, cells were plated in a T25 flask immediately after electroporation and media was exchanged for fresh RAW media after 1 h. Prior to imaging, cells were incubated with EDTA (17-711E; Lonza) for 10 min to detach them from the flask, spun for 90 s at 1173 rpm, and resuspended in 100 μL of media containing 23% Ficoll400 (F8016; Sigma Aldrich). .. A 4×5 mm glass cylinder (7030304; Bioptechs) was fixed to a glass bottom dish using silicon grease and 25 μL of cell suspension in 23% Ficoll was pipetted into the cylinder.

    Imaging:

    Article Title: Membrane flow drives an adhesion-independent amoeboid cell migration mode
    Article Snippet: .. For experiments performed in Ficoll, cells were plated in a T25 flask immediately after electroporation and media was exchanged for fresh RAW media after 1 h. Prior to imaging, cells were incubated with EDTA (17-711E; Lonza) for 10 min to detach them from the flask, spun for 90 s at 1173 rpm, and resuspended in 100 μL of media containing 23% Ficoll400 (F8016; Sigma Aldrich). .. A 4×5 mm glass cylinder (7030304; Bioptechs) was fixed to a glass bottom dish using silicon grease and 25 μL of cell suspension in 23% Ficoll was pipetted into the cylinder.

    Incubation:

    Article Title: Membrane flow drives an adhesion-independent amoeboid cell migration mode
    Article Snippet: .. For experiments performed in Ficoll, cells were plated in a T25 flask immediately after electroporation and media was exchanged for fresh RAW media after 1 h. Prior to imaging, cells were incubated with EDTA (17-711E; Lonza) for 10 min to detach them from the flask, spun for 90 s at 1173 rpm, and resuspended in 100 μL of media containing 23% Ficoll400 (F8016; Sigma Aldrich). .. A 4×5 mm glass cylinder (7030304; Bioptechs) was fixed to a glass bottom dish using silicon grease and 25 μL of cell suspension in 23% Ficoll was pipetted into the cylinder.

    Article Title: Hypoxic marker CA IX and adhesion mediator β-catenin are downregulated by lymphocytic choriomeningitis virus persistent infection
    Article Snippet: .. For flow cytometry the cells were washed 2 times for 10 min with PBS pH 7.2 and to all cells 1 ml of versene solution (Lonza, Verviers, Belgium) per well was added and incubated for 5 min at 4°C. ..

    other:

    Article Title: Critical role of the JNK-p53-GADD45? apoptotic cascade in mediating oxidative cytotoxicity in hippocampal neurons
    Article Snippet: The antibiotics solution containing 10 000 U·mL−1 penicillin and 10 mg·mL−1 streptomycin was obtained from Invitrogen (Carlsbad, CA), and trypsin-versene mixture (containing 0.25% trypsin and 0.02% EDTA) from Lonza Walkersville (Walkersville, MD, USA).

    Article Title: Effect of cAMP derivates on assembly and maintenance of tight junctions in human umbilical vein endothelial cells
    Article Snippet: To generate a calcium-free environment the cells were cultivated for 2 h in EGM medium in the presence of EDTA (3 μM) to chelate the free calcium or in PBS without any calcium but supplemented with all growth factors contained in the Lonza single quots.

    Flow Cytometry:

    Article Title: Hypoxic marker CA IX and adhesion mediator β-catenin are downregulated by lymphocytic choriomeningitis virus persistent infection
    Article Snippet: .. For flow cytometry the cells were washed 2 times for 10 min with PBS pH 7.2 and to all cells 1 ml of versene solution (Lonza, Verviers, Belgium) per well was added and incubated for 5 min at 4°C. ..

    Cytometry:

    Article Title: Hypoxic marker CA IX and adhesion mediator β-catenin are downregulated by lymphocytic choriomeningitis virus persistent infection
    Article Snippet: .. For flow cytometry the cells were washed 2 times for 10 min with PBS pH 7.2 and to all cells 1 ml of versene solution (Lonza, Verviers, Belgium) per well was added and incubated for 5 min at 4°C. ..

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  • 95
    Lonza trypsin versene mixture
    Trypsin Versene Mixture, supplied by Lonza, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsin versene mixture/product/Lonza
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypsin versene mixture - by Bioz Stars, 2021-05
    95/100 stars
      Buy from Supplier

    96
    Lonza trypsin versene
    Trypsin Versene, supplied by Lonza, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsin versene/product/Lonza
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypsin versene - by Bioz Stars, 2021-05
    96/100 stars
      Buy from Supplier

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