trypsin ethylenediamminetetraacetate  (Millipore)


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    Structured Review

    Millipore trypsin ethylenediamminetetraacetate
    Trypsin Ethylenediamminetetraacetate, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsin ethylenediamminetetraacetate/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypsin ethylenediamminetetraacetate - by Bioz Stars, 2020-04
    86/100 stars

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    Related Articles

    Microscopy:

    Article Title: The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress
    Article Snippet: For this purpose, briefly after separating the capsule under stereo microscope, seminiferous tubules were digested in two steps. .. Next, the tubes were centrifuged (100×g for 5 min), the supernatant was discarded, and the cells were resuspend in 1 ml trypsin-ethylenediamminetetraacetate (EDTA; Sigma) and 200 μg/ml DNaseI for 5 min in 37°C incubator.

    Incubation:

    Article Title: The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress
    Article Snippet: Next, the tubes were centrifuged (100×g for 5 min), the supernatant was discarded, and the cells were resuspend in 1 ml trypsin-ethylenediamminetetraacetate (EDTA; Sigma) and 200 μg/ml DNaseI for 5 min in 37°C incubator. .. The cells were washed in PBS solution and incubated in fixing buffer (1% paraformaldehyde solution).

    Cell Culture:

    Article Title: The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress
    Article Snippet: Digestion rats testes procedure After 50 days that equals with the duration of one spermatogenesis cycle of rat, testes of rats of all groups were removed and then testicular cells of them were cultured in Dulbecco's Modified Eagle Medium (DMEM) medium with 10% fetal bovine serum (FBS) and penicillin/streptomycin. .. Next, the tubes were centrifuged (100×g for 5 min), the supernatant was discarded, and the cells were resuspend in 1 ml trypsin-ethylenediamminetetraacetate (EDTA; Sigma) and 200 μg/ml DNaseI for 5 min in 37°C incubator.

    DNA Labeling:

    Article Title: The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress
    Article Snippet: Next, the tubes were centrifuged (100×g for 5 min), the supernatant was discarded, and the cells were resuspend in 1 ml trypsin-ethylenediamminetetraacetate (EDTA; Sigma) and 200 μg/ml DNaseI for 5 min in 37°C incubator. .. Next, the cells were centrifuged (300×g for 5 min), washed twice in PBS, resuspended in solution containing DNA-labeling, and then incubated (37°C) for 1 hour.

    Modification:

    Article Title: The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress
    Article Snippet: Digestion rats testes procedure After 50 days that equals with the duration of one spermatogenesis cycle of rat, testes of rats of all groups were removed and then testicular cells of them were cultured in Dulbecco's Modified Eagle Medium (DMEM) medium with 10% fetal bovine serum (FBS) and penicillin/streptomycin. .. Next, the tubes were centrifuged (100×g for 5 min), the supernatant was discarded, and the cells were resuspend in 1 ml trypsin-ethylenediamminetetraacetate (EDTA; Sigma) and 200 μg/ml DNaseI for 5 min in 37°C incubator.