trypsin edta solution  (Millipore)


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  • 99
    Name:
    Trypsin EDTA Solution 1X
    Description:

    Catalog Number:
    59417c
    Price:
    None
    Applications:
    The typical use for this product is in removing adherent cells from a culture surface.
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    Structured Review

    Millipore trypsin edta solution
    Both surface binding and intracellular entry by fusion of HIV-1 virions are enhanced by endosome inhibitors. SupT1 cells were pretreated for 30 min at 37°C with medium (columns 1 and 2), bafilomycin A1 (100 nM; columns 3 and 4), or NH 4 Cl (10 mM; columns 5 and 6). Cells were then incubated with GFP-Vpr-labeled HIV-1 virions (200 ng of p24 Gag) for 3 h at 37°C. Cells were either washed in <t>PBS</t> (columns 1, 3, and 5) or treated with a <t>trypsin-EDTA</t> solution to remove surface-bound virions (columns 2, 4, and 6) followed by flow cytometric analysis of living cells. The percentage of SupT1 cells displaying GFP epifluorescence is indicated on the ordinate. The data presented are from a representative experiment repeated three times with comparable results.

    https://www.bioz.com/result/trypsin edta solution/product/Millipore
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    trypsin edta solution - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Compensatory Link between Fusion and Endocytosis of Human Immunodeficiency Virus Type 1 in Human CD4 T Lymphocytes"

    Article Title: Compensatory Link between Fusion and Endocytosis of Human Immunodeficiency Virus Type 1 in Human CD4 T Lymphocytes

    Journal: Journal of Virology

    doi: 10.1128/JVI.78.3.1375-1383.2004

    Both surface binding and intracellular entry by fusion of HIV-1 virions are enhanced by endosome inhibitors. SupT1 cells were pretreated for 30 min at 37°C with medium (columns 1 and 2), bafilomycin A1 (100 nM; columns 3 and 4), or NH 4 Cl (10 mM; columns 5 and 6). Cells were then incubated with GFP-Vpr-labeled HIV-1 virions (200 ng of p24 Gag) for 3 h at 37°C. Cells were either washed in PBS (columns 1, 3, and 5) or treated with a trypsin-EDTA solution to remove surface-bound virions (columns 2, 4, and 6) followed by flow cytometric analysis of living cells. The percentage of SupT1 cells displaying GFP epifluorescence is indicated on the ordinate. The data presented are from a representative experiment repeated three times with comparable results.
    Figure Legend Snippet: Both surface binding and intracellular entry by fusion of HIV-1 virions are enhanced by endosome inhibitors. SupT1 cells were pretreated for 30 min at 37°C with medium (columns 1 and 2), bafilomycin A1 (100 nM; columns 3 and 4), or NH 4 Cl (10 mM; columns 5 and 6). Cells were then incubated with GFP-Vpr-labeled HIV-1 virions (200 ng of p24 Gag) for 3 h at 37°C. Cells were either washed in PBS (columns 1, 3, and 5) or treated with a trypsin-EDTA solution to remove surface-bound virions (columns 2, 4, and 6) followed by flow cytometric analysis of living cells. The percentage of SupT1 cells displaying GFP epifluorescence is indicated on the ordinate. The data presented are from a representative experiment repeated three times with comparable results.

    Techniques Used: Binding Assay, Incubation, Labeling, Flow Cytometry

    Flow cytometric analysis of PHA-activated human lymphoblasts infected with GFP-Vpr-labeled HIV-1 virions. (A) PBMCs activated with PHA and cultured in interleukin-2 for 4 days were inoculated with GFP-Vpr-labeled X4-tropic HIV-1 (300 ng of p24 Gag) at 37 or 4°C as indicated. After 3 h, the cells were washed at room temperature with PBS (−T) or a trypsin-EDTA solution (+T) to remove surface-bound virions. Live cells were then analyzed by flow cytometry to detect GFP epifluorescence. Error bars indicate standard deviations of the mean derived from two independent experiments. (B and C) PHA-activated human lymphoblasts were pretreated for 30 min at 37°C with either medium (panels 2 and 4) or AMD3100 (panels 3 and 5). Cells were then incubated at 37°C for 3 or 24 h with GFP-Vpr-labeled X4-tropic HIV-1 or HIV virions pseudotyped with the VSV-G envelope (200 ng of p24 Gag) at 37°C. Cells were subsequently stained with APC-conjugated CD4 antibodies (B) or PE-conjugated CD4 antibodies (C). Cells were analyzed for GFP epifluorescence and APC or PE immunofluorescence. The percentage of cells in each quadrant is indicated. Data shown are from a representative experiment performed three times with comparable results. Note preferential entry of HIV into CD4 cells and the absence of inhibitory effects of AMD3100 measured either at 3 or 24 h.
    Figure Legend Snippet: Flow cytometric analysis of PHA-activated human lymphoblasts infected with GFP-Vpr-labeled HIV-1 virions. (A) PBMCs activated with PHA and cultured in interleukin-2 for 4 days were inoculated with GFP-Vpr-labeled X4-tropic HIV-1 (300 ng of p24 Gag) at 37 or 4°C as indicated. After 3 h, the cells were washed at room temperature with PBS (−T) or a trypsin-EDTA solution (+T) to remove surface-bound virions. Live cells were then analyzed by flow cytometry to detect GFP epifluorescence. Error bars indicate standard deviations of the mean derived from two independent experiments. (B and C) PHA-activated human lymphoblasts were pretreated for 30 min at 37°C with either medium (panels 2 and 4) or AMD3100 (panels 3 and 5). Cells were then incubated at 37°C for 3 or 24 h with GFP-Vpr-labeled X4-tropic HIV-1 or HIV virions pseudotyped with the VSV-G envelope (200 ng of p24 Gag) at 37°C. Cells were subsequently stained with APC-conjugated CD4 antibodies (B) or PE-conjugated CD4 antibodies (C). Cells were analyzed for GFP epifluorescence and APC or PE immunofluorescence. The percentage of cells in each quadrant is indicated. Data shown are from a representative experiment performed three times with comparable results. Note preferential entry of HIV into CD4 cells and the absence of inhibitory effects of AMD3100 measured either at 3 or 24 h.

    Techniques Used: Flow Cytometry, Infection, Labeling, Cell Culture, Cytometry, Derivative Assay, Incubation, Staining, Immunofluorescence

    Flow cytometric analysis of human SupT1 cells incubated with GFP-Vpr-labeled X4-tropic (A) or R5-tropic (B) HIV-1 virions in the presence of medium (panels 2 and 3), neutralizing anti-CD4 antibodies (panels 4 and 5), or AMD3100 (panels 6 and 7). SupT1 T cells were preincubated in the presence or absence of anti-CD4 antibodies or AMD3100 for 30 min at 37°C. Cells were then inoculated with GFP-Vpr-labeled X4-tropic HIV-1 or CCR5-tropic HIV-1 R5 (200 ng of p24 Gag) for 3 h at 37°C. The cells were subsequently washed with PBS (panels 1, 2, 4, and 6) or treated with a trypsin-EDTA solution (panels 3, 5, and 7) to remove surface-bound virions. Live cells were then analyzed by flow cytometry for GFP epifluorescence. The vertical bar indicates a gate established by analysis of uninfected cells (panels 1). The percentage of GFP-positive cells is indicated in the upper right hand corner of each panel. The anti-CD4 antibodies significantly inhibited the entry of both X4-tropic and R5-tropic virions, whereas AMD3100 unexpectedly failed to reduce the overall entry of X4-tropic virions.
    Figure Legend Snippet: Flow cytometric analysis of human SupT1 cells incubated with GFP-Vpr-labeled X4-tropic (A) or R5-tropic (B) HIV-1 virions in the presence of medium (panels 2 and 3), neutralizing anti-CD4 antibodies (panels 4 and 5), or AMD3100 (panels 6 and 7). SupT1 T cells were preincubated in the presence or absence of anti-CD4 antibodies or AMD3100 for 30 min at 37°C. Cells were then inoculated with GFP-Vpr-labeled X4-tropic HIV-1 or CCR5-tropic HIV-1 R5 (200 ng of p24 Gag) for 3 h at 37°C. The cells were subsequently washed with PBS (panels 1, 2, 4, and 6) or treated with a trypsin-EDTA solution (panels 3, 5, and 7) to remove surface-bound virions. Live cells were then analyzed by flow cytometry for GFP epifluorescence. The vertical bar indicates a gate established by analysis of uninfected cells (panels 1). The percentage of GFP-positive cells is indicated in the upper right hand corner of each panel. The anti-CD4 antibodies significantly inhibited the entry of both X4-tropic and R5-tropic virions, whereas AMD3100 unexpectedly failed to reduce the overall entry of X4-tropic virions.

    Techniques Used: Flow Cytometry, Incubation, Labeling, Cytometry

    Related Articles

    MTT Assay:

    Article Title: Galangin and Pinocembrin from Propolis Ameliorate Insulin Resistance in HepG2 Cells via Regulating Akt/mTOR Signaling
    Article Snippet: .. Dimethyl sulfoxide (DMSO), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), trypsin-EDTA solution 1× (0.25% trypsin, 0.02% EDTA), insulin solution (human), bicinchoninic acid (BAC) protein assay kit, pinobanksin, galangin, chrysin, and pinocembrin were obtained from Sigma-Aldrich (St. Louis, MO, USA). .. Fetal bovine serum (FBS) was obtained from Gibco (Grand Island, NY, USA).

    In Vitro:

    Article Title: Stimulatory Effect of Autologous Adipose Tissue-Derived Stromal Cells in an Atelocollagen Matrix on Wound Healing in Diabetic db/db Mice
    Article Snippet: .. For the in vitro experiments, the cells were released from the culture dish by treatment with a trypsin-EDTA solution (1×; 0.2 g EDTA and 0.5 g porcine trypsin per liter of HBSS; Sigma Aldrich Japan, Tokyo) at 37°C for 5 min, and the suspension was centrifuged to pellet the cells. ..

    Dissection:

    Article Title: Differential expression of sirtuin family members in the developing, adult, and aged rat brain
    Article Snippet: .. Cortices were dissected from whole brains using micro dissection forceps, and the collected tissue was incubated in 2 ml of 0.05% trypsin (Sigma–Aldrich, St. Louis, MO, cat # 59417C) at 37°C for 15 min. .. The tissue was then triturated by glass pipette 10–15 times to disperse the cells in seeding medium (Dulbecco's Modified Eagle Medium (DMEM) /F-12) (Invitrogen, cat # 21041025) containing 10% HS and then centrifuged for 5 min at 21,000 × g to pellet the cells.

    Time-lapse Microscopy:

    Article Title: A Novel Cell Force Sensor for Quantification of Traction during Cell Spreading and Contact Guidance
    Article Snippet: .. For time-lapse microscopy, cells were detached with 1× trypsin-EDTA solution (Sigma, St. Louis, MO) and plated, as per routine passage. ..

    Incubation:

    Article Title: Vibrio anguillarum Is Genetically and Phenotypically Unaffected by Long-Term Continuous Exposure to the Antibacterial Compound Tropodithietic Acid
    Article Snippet: .. HBSS was removed, and the cells were trypsinized with 1 ml trypsin-EDTA (catalogue no. 59428C; Sigma) and incubated for 10 min. .. Two hundred microliters of CHSE-214 cell suspension (containing 1,000 to 10,000 cells ml−1 ) was transferred into each well of a six-well test plate with 3 ml fresh medium.

    Article Title: Differential expression of sirtuin family members in the developing, adult, and aged rat brain
    Article Snippet: .. Cortices were dissected from whole brains using micro dissection forceps, and the collected tissue was incubated in 2 ml of 0.05% trypsin (Sigma–Aldrich, St. Louis, MO, cat # 59417C) at 37°C for 15 min. .. The tissue was then triturated by glass pipette 10–15 times to disperse the cells in seeding medium (Dulbecco's Modified Eagle Medium (DMEM) /F-12) (Invitrogen, cat # 21041025) containing 10% HS and then centrifuged for 5 min at 21,000 × g to pellet the cells.

    BAC Assay:

    Article Title: Galangin and Pinocembrin from Propolis Ameliorate Insulin Resistance in HepG2 Cells via Regulating Akt/mTOR Signaling
    Article Snippet: .. Dimethyl sulfoxide (DMSO), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), trypsin-EDTA solution 1× (0.25% trypsin, 0.02% EDTA), insulin solution (human), bicinchoninic acid (BAC) protein assay kit, pinobanksin, galangin, chrysin, and pinocembrin were obtained from Sigma-Aldrich (St. Louis, MO, USA). .. Fetal bovine serum (FBS) was obtained from Gibco (Grand Island, NY, USA).

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  • 99
    Millipore trypsin edta solution
    Trypsin Edta Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsin edta solution/product/Millipore
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    trypsin edta solution - by Bioz Stars, 2020-09
    99/100 stars
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    Millipore in solution trypsin
    In Solution Trypsin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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