trypsin edta solution 10x  (Millipore)


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  • 94
    Name:
    Trypsin EDTA Solution 10X
    Description:

    Catalog Number:
    59418c
    Price:
    None
    Applications:
    Trypsin-EDTA Solution 10X has been used to release adherent cells from tissue culture plates for passaging.
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    Structured Review

    Millipore trypsin edta solution 10x

    https://www.bioz.com/result/trypsin edta solution 10x/product/Millipore
    Average 94 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    trypsin edta solution 10x - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    Isolation:

    Article Title: Use of a special bioreactor for the cultivation of a new flexible polyurethane scaffold for aortic valve tissue engineering
    Article Snippet: .. For EC isolation, segments were incubated with trypsin/EDTA-solution (10x; Sigma-Aldrich GmbH, Taufkirchen, Germany) for 25 min at 37°C / 5% CO2. .. For FBs isolation, veins were subsequently flushed with 2 mg/ml collagenase type II (Worthington Biochemical Corporation / CellSystems GmbH, St. Katharinen, Germany) in human serum albumin (200 g/l; Baxter GmbH, Unterschleißheim, Germany) and incubated for 30 min.

    Incubation:

    Article Title: Areca nut extract demonstrated apoptosis-inducing mechanism by increased caspase-3 activities on oral squamous cell carcinoma
    Article Snippet: .. The medium was disposed and rinsed with PBS Ca2+ and Mg2+ two to three times with the volume of 2 mL, then 1 mL Trypsin EDTA (59418C, Sigma-Aldrich) was added, then it was incubated for five to ten minutes. ..

    Article Title: Use of a special bioreactor for the cultivation of a new flexible polyurethane scaffold for aortic valve tissue engineering
    Article Snippet: .. For EC isolation, segments were incubated with trypsin/EDTA-solution (10x; Sigma-Aldrich GmbH, Taufkirchen, Germany) for 25 min at 37°C / 5% CO2. .. For FBs isolation, veins were subsequently flushed with 2 mg/ml collagenase type II (Worthington Biochemical Corporation / CellSystems GmbH, St. Katharinen, Germany) in human serum albumin (200 g/l; Baxter GmbH, Unterschleißheim, Germany) and incubated for 30 min.

    Transfection:

    Article Title: Mob2 Insufficiency Disrupts Neuronal Migration in the Developing Cortex
    Article Snippet: .. Once at 80% confluency, cells were treated with 0.5% trypsin (Sigma-Aldrich, Cat. # 59418C) and reverse transfection protocol was carried out in a 24-well plate with cells re-plated at the appropriate densities. .. Media contained DMEM, 10% FCS and 1% L-glutamine and 30 nM siRNA scrambled or against MOB2 (Sigma-Aldrich, Cat. # SIC001-10NMOL and SASI_Hs01_00177399) pre-mixed in Lipofectamine RNAiMAX (Invitrogen, Cat. # 13778-075) according to the manufacturer’s instructions.

    Cell Culture:

    Article Title: ALCAM (CD166) as a gene expression marker for human mesenchymal stromal cell characterisation
    Article Snippet: .. The hMSCs and hDFs cultured under different oxygen levels (Supplemental Table 1) were harvested with trypsin solution (59418C, Sigma-Aldrich, Dorset, UK) and collected into a pellet. .. Trizol (11596-018, ThermoFisher) was added onto the cell pellet and resuspended.

    Article Title: Solid-Phase Immunoglobulins IgG and IgM Activate Macrophages with Solid-Phase IgM Acting via a Novel Scavenger Receptor A Pathway
    Article Snippet: .. HEK293 cells were grown under standard cell culture conditions in 10% FCS DMEM and detached by digestion with trypsin solution (59418C, Sigma-Aldrich), creating a cell suspension. ..

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  • 94
    Millipore 10x solution
    10x Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x solution/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    10x solution - by Bioz Stars, 2020-09
    94/100 stars
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