trypsin digestion buffer  (Millipore)


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    Structured Review

    Millipore trypsin digestion buffer
    Trypsin Digestion Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsin digestion buffer/product/Millipore
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    trypsin digestion buffer - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Glycomic Signatures on Serum IgGs for Prediction of Postvaccination Response
    Article Snippet: IgG purification from serum with Protein G Total IgGs were isolated from human serum with Protein G Sepharose™ 4 Fast Flow beads (90 μm; GE Healthcare, Uppsala, Sweden). .. The buffer from the captured IgGs was exchanged with trypsin digestion buffer (50 mM ammonium bicarbonate in 15% acetonitrile, pH 7.4) and concentrated to a final volume of 30 μL with a 30 K centrifugal filter unit (Millipore, Carrigtwchill, County Cork, Ireland).

    Sequencing:

    Article Title: Identification and characterization of lysine-rich proteins and starch biosynthesis genes in the opaque2 mutant by transcriptional and proteomic analysis
    Article Snippet: .. The gel pieces were dried in a Speed-Vac drier for 1 h, and were rehydrated by incubating in trypsin digestion buffer (50 mM ammonium bicarbonate, 5 mM CaCl2 , 15 μg/ml sequencing grade trypsin (Sigma, St. Louis, MO)) for 10 minutes. .. Excess buffer was removed and enough trypsin-free digestion buffer was added to barely cover the gel pieces; the samples were then incubated overnight at 37°C.

    Article Title: Structural basis for host recognition by the Haemophilus influenzae Hia autotransporter
    Article Snippet: .. To assess susceptibility to trypsin of HiaBD1 derivatives, 15 μg of purified protein was incubated with 250 ng of sequencing grade modified trypsin (Sigma #1-521-187) in trypsin digestion buffer (50 mM Tris–HCl pH 7.5, 0.1 M NaCl, 1 mM EDTA). ..

    Isolation:

    Article Title: Glycomic Signatures on Serum IgGs for Prediction of Postvaccination Response
    Article Snippet: IgG purification from serum with Protein G Total IgGs were isolated from human serum with Protein G Sepharose™ 4 Fast Flow beads (90 μm; GE Healthcare, Uppsala, Sweden). .. The buffer from the captured IgGs was exchanged with trypsin digestion buffer (50 mM ammonium bicarbonate in 15% acetonitrile, pH 7.4) and concentrated to a final volume of 30 μL with a 30 K centrifugal filter unit (Millipore, Carrigtwchill, County Cork, Ireland).

    Purification:

    Article Title: Glycomic Signatures on Serum IgGs for Prediction of Postvaccination Response
    Article Snippet: Paragraph title: IgG purification from serum with Protein G ... The buffer from the captured IgGs was exchanged with trypsin digestion buffer (50 mM ammonium bicarbonate in 15% acetonitrile, pH 7.4) and concentrated to a final volume of 30 μL with a 30 K centrifugal filter unit (Millipore, Carrigtwchill, County Cork, Ireland).

    Article Title: Structural basis for host recognition by the Haemophilus influenzae Hia autotransporter
    Article Snippet: .. To assess susceptibility to trypsin of HiaBD1 derivatives, 15 μg of purified protein was incubated with 250 ng of sequencing grade modified trypsin (Sigma #1-521-187) in trypsin digestion buffer (50 mM Tris–HCl pH 7.5, 0.1 M NaCl, 1 mM EDTA). ..

    Acrylamide Gel Assay:

    Article Title: Identification and characterization of lysine-rich proteins and starch biosynthesis genes in the opaque2 mutant by transcriptional and proteomic analysis
    Article Snippet: Protein identification Protein spots of interest were excised from the acrylamide gel and digested with trypsin using an in-gel digestion procedure. .. The gel pieces were dried in a Speed-Vac drier for 1 h, and were rehydrated by incubating in trypsin digestion buffer (50 mM ammonium bicarbonate, 5 mM CaCl2 , 15 μg/ml sequencing grade trypsin (Sigma, St. Louis, MO)) for 10 minutes.

    Incubation:

    Article Title: Identification and characterization of lysine-rich proteins and starch biosynthesis genes in the opaque2 mutant by transcriptional and proteomic analysis
    Article Snippet: The gel pieces were dried in a Speed-Vac drier for 1 h, and were rehydrated by incubating in trypsin digestion buffer (50 mM ammonium bicarbonate, 5 mM CaCl2 , 15 μg/ml sequencing grade trypsin (Sigma, St. Louis, MO)) for 10 minutes. .. Excess buffer was removed and enough trypsin-free digestion buffer was added to barely cover the gel pieces; the samples were then incubated overnight at 37°C.

    Article Title: Glycomic Signatures on Serum IgGs for Prediction of Postvaccination Response
    Article Snippet: The mixture including the beads, 250 μL of binding buffer, and 10 μL of serum was then mixed by inversion and incubated at room temperature for 1 h. After incubation, the beads were washed twice with 250 μL of binding buffer. .. The buffer from the captured IgGs was exchanged with trypsin digestion buffer (50 mM ammonium bicarbonate in 15% acetonitrile, pH 7.4) and concentrated to a final volume of 30 μL with a 30 K centrifugal filter unit (Millipore, Carrigtwchill, County Cork, Ireland).

    Article Title: Structural basis for host recognition by the Haemophilus influenzae Hia autotransporter
    Article Snippet: .. To assess susceptibility to trypsin of HiaBD1 derivatives, 15 μg of purified protein was incubated with 250 ng of sequencing grade modified trypsin (Sigma #1-521-187) in trypsin digestion buffer (50 mM Tris–HCl pH 7.5, 0.1 M NaCl, 1 mM EDTA). ..

    Modification:

    Article Title: Structural basis for host recognition by the Haemophilus influenzae Hia autotransporter
    Article Snippet: .. To assess susceptibility to trypsin of HiaBD1 derivatives, 15 μg of purified protein was incubated with 250 ng of sequencing grade modified trypsin (Sigma #1-521-187) in trypsin digestion buffer (50 mM Tris–HCl pH 7.5, 0.1 M NaCl, 1 mM EDTA). ..

    Binding Assay:

    Article Title: Glycomic Signatures on Serum IgGs for Prediction of Postvaccination Response
    Article Snippet: The mixture including the beads, 250 μL of binding buffer, and 10 μL of serum was then mixed by inversion and incubated at room temperature for 1 h. After incubation, the beads were washed twice with 250 μL of binding buffer. .. The buffer from the captured IgGs was exchanged with trypsin digestion buffer (50 mM ammonium bicarbonate in 15% acetonitrile, pH 7.4) and concentrated to a final volume of 30 μL with a 30 K centrifugal filter unit (Millipore, Carrigtwchill, County Cork, Ireland).

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  • 99
    Millipore trypsin digestion buffer
    Trypsin Digestion Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsin digestion buffer/product/Millipore
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    trypsin digestion buffer - by Bioz Stars, 2020-02
    99/100 stars
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