truseq rna sample preparation kit  (Illumina Inc)

 
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    Name:
    TruSeq RNA Library Preparation Kit v2
    Description:
    Generate mRNA focused sequencing libraries from total RNA with enhanced multiplex capability and a simple workflow with master mixed reagents Multiplex Capabilities Kits feature 24 unique indexes delivering enhanced multiplex performance for processing large numbers of samples The kits include adapters containing unique index sequences that are ligated to sample fragments at the beginning of the library construction process This allows the samples to be pooled and then individually identified during downstream analysis Minimal Hands On Steps Master mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean up as compared to previous methods minimizing hands on time This results in economical high throughput RNA sequencing studies achieved with a user friendly workflow Automation Options Find an up to date list of automation vendors with robotic systems compatible with this kit
    Catalog Number:
    rs-122-2001
    Price:
    None
    Category:
    Library Preparation Kits
    Buy from Supplier


    Structured Review

    Illumina Inc truseq rna sample preparation kit
    TruSeq RNA Library Preparation Kit v2
    Generate mRNA focused sequencing libraries from total RNA with enhanced multiplex capability and a simple workflow with master mixed reagents Multiplex Capabilities Kits feature 24 unique indexes delivering enhanced multiplex performance for processing large numbers of samples The kits include adapters containing unique index sequences that are ligated to sample fragments at the beginning of the library construction process This allows the samples to be pooled and then individually identified during downstream analysis Minimal Hands On Steps Master mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean up as compared to previous methods minimizing hands on time This results in economical high throughput RNA sequencing studies achieved with a user friendly workflow Automation Options Find an up to date list of automation vendors with robotic systems compatible with this kit
    https://www.bioz.com/result/truseq rna sample preparation kit/product/Illumina Inc
    Average 99 stars, based on 1566 article reviews
    Price from $9.99 to $1999.99
    truseq rna sample preparation kit - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Normalization of human RNA-seq experiments using chimpanzee RNA as a spike-in standard"

    Article Title: Normalization of human RNA-seq experiments using chimpanzee RNA as a spike-in standard

    Journal: Scientific Reports

    doi: 10.1038/srep31923

    Improved comparability across degradation effects and library preparation methods. ( a ) Correlation of unnormalized reads from normally processed iPSC RNA (HICL) and in-process degraded iPSC RNA (HICL_D). Reads for 51,552 loci over the read cutoff of 40 were plotted. ( b ) Correlation of SNV-normalized reads from the HICL and HICL_D samples. ( c ) Agreement of estimates for gene expression in HICL and HICL_D samples estimated by unnormalized reads and SNV-normalized reads. ( d ) Correlation of unnormalized reads generated by TruSeq method (HICL) and NexTera method (HICL_N). Reads for 53,585 loci were plotted. ( e ) Correlation of SNV-normalized reads from the HICL and HICL_N samples. ( f ) Agreement of estimates for gene expression in HICL and HICL_N samples estimated by unnormalized reads and SNV-normalized reads.
    Figure Legend Snippet: Improved comparability across degradation effects and library preparation methods. ( a ) Correlation of unnormalized reads from normally processed iPSC RNA (HICL) and in-process degraded iPSC RNA (HICL_D). Reads for 51,552 loci over the read cutoff of 40 were plotted. ( b ) Correlation of SNV-normalized reads from the HICL and HICL_D samples. ( c ) Agreement of estimates for gene expression in HICL and HICL_D samples estimated by unnormalized reads and SNV-normalized reads. ( d ) Correlation of unnormalized reads generated by TruSeq method (HICL) and NexTera method (HICL_N). Reads for 53,585 loci were plotted. ( e ) Correlation of SNV-normalized reads from the HICL and HICL_N samples. ( f ) Agreement of estimates for gene expression in HICL and HICL_N samples estimated by unnormalized reads and SNV-normalized reads.

    Techniques Used: Expressing, Generated

    2) Product Images from "IVT-seq reveals extreme bias in RNA sequencing"

    Article Title: IVT-seq reveals extreme bias in RNA sequencing

    Journal: Genome Biology

    doi: 10.1186/gb-2014-15-6-r86

    Construction of IVT-seq libraries. (A) Preparation of a pool of 1,062 human cDNA plasmids. Contents of three 384-well plates containing MGC plasmids were pooled together. Pool was amplified via transformation in Escherichia coli , and resulting clones were purified and re-pooled. (B) Generation of IVT transcripts. Pool of MGC plasmids was linearized and used as a template for an in vitro transcription reaction. Enzymes and unincorporated nucleotides were purified, leaving pool of polyA transcripts. (C) Creation of IVT-seq libraries. Listed quantities of IVT RNA were mixed with mouse liver total RNA to create six pools with final RNA quantities of 1 μg. Ribosomal RNA was depleted from these pools using the Ribo-Zero Gold kit. IVT RNA and mouse RNA are now present in pools at the listed ratios, following depletion of rRNA from mouse total RNA. These pools were used to generate RNA-seq libraries using Illumina’s TruSeq kit/protocol. This entire process was performed in duplicate. Replicate libraries were pooled separately and sequenced in separate HiSeq 2000 lanes (two lanes total). IVT, in vitro transcribed; MGC, Mammalian Gene Collection.
    Figure Legend Snippet: Construction of IVT-seq libraries. (A) Preparation of a pool of 1,062 human cDNA plasmids. Contents of three 384-well plates containing MGC plasmids were pooled together. Pool was amplified via transformation in Escherichia coli , and resulting clones were purified and re-pooled. (B) Generation of IVT transcripts. Pool of MGC plasmids was linearized and used as a template for an in vitro transcription reaction. Enzymes and unincorporated nucleotides were purified, leaving pool of polyA transcripts. (C) Creation of IVT-seq libraries. Listed quantities of IVT RNA were mixed with mouse liver total RNA to create six pools with final RNA quantities of 1 μg. Ribosomal RNA was depleted from these pools using the Ribo-Zero Gold kit. IVT RNA and mouse RNA are now present in pools at the listed ratios, following depletion of rRNA from mouse total RNA. These pools were used to generate RNA-seq libraries using Illumina’s TruSeq kit/protocol. This entire process was performed in duplicate. Replicate libraries were pooled separately and sequenced in separate HiSeq 2000 lanes (two lanes total). IVT, in vitro transcribed; MGC, Mammalian Gene Collection.

    Techniques Used: Amplification, Transformation Assay, Clone Assay, Purification, In Vitro, RNA Sequencing Assay

    3) Product Images from "IVT-seq reveals extreme bias in RNA sequencing"

    Article Title: IVT-seq reveals extreme bias in RNA sequencing

    Journal: Genome Biology

    doi: 10.1186/gb-2014-15-6-r86

    Construction of IVT-seq libraries. (A) Preparation of a pool of 1,062 human cDNA plasmids. Contents of three 384-well plates containing MGC plasmids were pooled together. Pool was amplified via transformation in Escherichia coli , and resulting clones were purified and re-pooled. (B) Generation of IVT transcripts. Pool of MGC plasmids was linearized and used as a template for an in vitro transcription reaction. Enzymes and unincorporated nucleotides were purified, leaving pool of polyA transcripts. (C) Creation of IVT-seq libraries. Listed quantities of IVT RNA were mixed with mouse liver total RNA to create six pools with final RNA quantities of 1 μg. Ribosomal RNA was depleted from these pools using the Ribo-Zero Gold kit. IVT RNA and mouse RNA are now present in pools at the listed ratios, following depletion of rRNA from mouse total RNA. These pools were used to generate RNA-seq libraries using Illumina’s TruSeq kit/protocol. This entire process was performed in duplicate. Replicate libraries were pooled separately and sequenced in separate HiSeq 2000 lanes (two lanes total). IVT, in vitro transcribed; MGC, Mammalian Gene Collection.
    Figure Legend Snippet: Construction of IVT-seq libraries. (A) Preparation of a pool of 1,062 human cDNA plasmids. Contents of three 384-well plates containing MGC plasmids were pooled together. Pool was amplified via transformation in Escherichia coli , and resulting clones were purified and re-pooled. (B) Generation of IVT transcripts. Pool of MGC plasmids was linearized and used as a template for an in vitro transcription reaction. Enzymes and unincorporated nucleotides were purified, leaving pool of polyA transcripts. (C) Creation of IVT-seq libraries. Listed quantities of IVT RNA were mixed with mouse liver total RNA to create six pools with final RNA quantities of 1 μg. Ribosomal RNA was depleted from these pools using the Ribo-Zero Gold kit. IVT RNA and mouse RNA are now present in pools at the listed ratios, following depletion of rRNA from mouse total RNA. These pools were used to generate RNA-seq libraries using Illumina’s TruSeq kit/protocol. This entire process was performed in duplicate. Replicate libraries were pooled separately and sequenced in separate HiSeq 2000 lanes (two lanes total). IVT, in vitro transcribed; MGC, Mammalian Gene Collection.

    Techniques Used: Amplification, Transformation Assay, Clone Assay, Purification, In Vitro, RNA Sequencing Assay

    4) Product Images from "IVT-seq reveals extreme bias in RNA sequencing"

    Article Title: IVT-seq reveals extreme bias in RNA sequencing

    Journal: Genome Biology

    doi: 10.1186/gb-2014-15-6-r86

    Construction of IVT-seq libraries. (A) Preparation of a pool of 1,062 human cDNA plasmids. Contents of three 384-well plates containing MGC plasmids were pooled together. Pool was amplified via transformation in Escherichia coli , and resulting clones were purified and re-pooled. (B) Generation of IVT transcripts. Pool of MGC plasmids was linearized and used as a template for an in vitro transcription reaction. Enzymes and unincorporated nucleotides were purified, leaving pool of polyA transcripts. (C) Creation of IVT-seq libraries. Listed quantities of IVT RNA were mixed with mouse liver total RNA to create six pools with final RNA quantities of 1 μg. Ribosomal RNA was depleted from these pools using the Ribo-Zero Gold kit. IVT RNA and mouse RNA are now present in pools at the listed ratios, following depletion of rRNA from mouse total RNA. These pools were used to generate RNA-seq libraries using Illumina’s TruSeq kit/protocol. This entire process was performed in duplicate. Replicate libraries were pooled separately and sequenced in separate HiSeq 2000 lanes (two lanes total). IVT, in vitro transcribed; MGC, Mammalian Gene Collection.
    Figure Legend Snippet: Construction of IVT-seq libraries. (A) Preparation of a pool of 1,062 human cDNA plasmids. Contents of three 384-well plates containing MGC plasmids were pooled together. Pool was amplified via transformation in Escherichia coli , and resulting clones were purified and re-pooled. (B) Generation of IVT transcripts. Pool of MGC plasmids was linearized and used as a template for an in vitro transcription reaction. Enzymes and unincorporated nucleotides were purified, leaving pool of polyA transcripts. (C) Creation of IVT-seq libraries. Listed quantities of IVT RNA were mixed with mouse liver total RNA to create six pools with final RNA quantities of 1 μg. Ribosomal RNA was depleted from these pools using the Ribo-Zero Gold kit. IVT RNA and mouse RNA are now present in pools at the listed ratios, following depletion of rRNA from mouse total RNA. These pools were used to generate RNA-seq libraries using Illumina’s TruSeq kit/protocol. This entire process was performed in duplicate. Replicate libraries were pooled separately and sequenced in separate HiSeq 2000 lanes (two lanes total). IVT, in vitro transcribed; MGC, Mammalian Gene Collection.

    Techniques Used: Amplification, Transformation Assay, Clone Assay, Purification, In Vitro, RNA Sequencing Assay

    Related Articles

    Multiplex Assay:

    Article Title: Depletion of tRNA-halves enables effective small RNA sequencing of low-input murine serum samples
    Article Snippet: .. Small RNA sequencing For Small RNA library preparation we used the TailorMix miRNA sample preparation kit v2 (Seqmatic), the NEBNext Multiplex Small RNA library prep kit (New England Biolabs) or the TruSeq small RNA library preparation kit v2 (Illumina) following manufacturer’s instructions with small modifications ( ). ..

    Sample Prep:

    Article Title: Normalization of human RNA-seq experiments using chimpanzee RNA as a spike-in standard
    Article Snippet: .. After second strand synthesis using the second strand master mix in the TruSeq RNA Sample Preparation Kit, cDNA was subjected to the library preparation procedure using NexTera DNA Sample Preparation Kit (Illumina). ..

    Article Title: Normalization of human RNA-seq experiments using chimpanzee RNA as a spike-in standard
    Article Snippet: .. Preparation of NGS library Normal NGS library was constructed using TruSeq RNA Sample Preparation Kit v2 (Illumina) following the manufacturer’s instruction starting from 2 μg of total RNA. .. NexTera library was constructed following the protocol in TotalScript RNA-Seq Kit (Epicentre) with a few modifications.

    Article Title: Low-cost, low-input RNA-seq protocols perform nearly as well as high-input protocols
    Article Snippet: .. TruSeq libraries were prepared with the “TruSeq RNA Sample Preparation Kit v2” (Illumina Cat.#RS-122-2001) according to manufacturer instructions, except for the following modifications. .. We find that this increases the effective concentration of RNA and cDNA.

    Next-Generation Sequencing:

    Article Title: Normalization of human RNA-seq experiments using chimpanzee RNA as a spike-in standard
    Article Snippet: .. Preparation of NGS library Normal NGS library was constructed using TruSeq RNA Sample Preparation Kit v2 (Illumina) following the manufacturer’s instruction starting from 2 μg of total RNA. .. NexTera library was constructed following the protocol in TotalScript RNA-Seq Kit (Epicentre) with a few modifications.

    Article Title: RNA-seq mixology: designing realistic control experiments to compare protocols and analysis methods
    Article Snippet: .. A total of 10 μl (∼1 μg) from each replicated mixture (both good and degraded) were used for Next Generation Sequencing library preparation using two different protocols: Illumina's TruSeq Total Stranded RNA kit with Ribozero depletion and Illumina's TruSeq RNA v2 kit. .. Libraries were quantified and normalized by qPCR, as recommended by Illumina, and libraries prepared using the same protocol were pooled together.

    Construct:

    Article Title: A comparative analysis of library prep approaches for sequencing low input translatome samples
    Article Snippet: .. Ovation® RNA-Seq system V2 combined with TruSeq RNA library prep kit v2 We performed a hybrid library preparation by using Ovation® RNA-Seq System V2 (NuGEN, San Carlos, CA, USA) to synthesize cDNA and the TruSeq RNA Library Preparation Kit v2 to construct the sequencing library (Illumina, San Diego, CA, USA), consistent with the NuGEN manufacturer protocol (See Additional file : Table S1). .. Briefly, 4 ng of total RNA or RiboTag IP RNA were used to synthesize cDNA following the NuGEN’s instructions.

    Article Title: Normalization of human RNA-seq experiments using chimpanzee RNA as a spike-in standard
    Article Snippet: .. Preparation of NGS library Normal NGS library was constructed using TruSeq RNA Sample Preparation Kit v2 (Illumina) following the manufacturer’s instruction starting from 2 μg of total RNA. .. NexTera library was constructed following the protocol in TotalScript RNA-Seq Kit (Epicentre) with a few modifications.

    Article Title: A comparative analysis of library prep approaches for sequencing low input translatome samples
    Article Snippet: .. We performed a hybrid library preparation by using Ovation® RNA-Seq System V2 (NuGEN, San Carlos, CA, USA) to synthesize cDNA and the TruSeq RNA Library Preparation Kit v2 to construct the sequencing library (Illumina, San Diego, CA, USA), consistent with the NuGEN manufacturer protocol (See Additional file : Table S1). .. Briefly, 4 ng of total RNA or RiboTag IP RNA were used to synthesize cDNA following the NuGEN’s instructions.

    Sequencing:

    Article Title: A comparative analysis of library prep approaches for sequencing low input translatome samples
    Article Snippet: .. Ovation® RNA-Seq system V2 combined with TruSeq RNA library prep kit v2 We performed a hybrid library preparation by using Ovation® RNA-Seq System V2 (NuGEN, San Carlos, CA, USA) to synthesize cDNA and the TruSeq RNA Library Preparation Kit v2 to construct the sequencing library (Illumina, San Diego, CA, USA), consistent with the NuGEN manufacturer protocol (See Additional file : Table S1). .. Briefly, 4 ng of total RNA or RiboTag IP RNA were used to synthesize cDNA following the NuGEN’s instructions.

    Article Title: A comparative analysis of library prep approaches for sequencing low input translatome samples
    Article Snippet: .. We performed a hybrid library preparation by using Ovation® RNA-Seq System V2 (NuGEN, San Carlos, CA, USA) to synthesize cDNA and the TruSeq RNA Library Preparation Kit v2 to construct the sequencing library (Illumina, San Diego, CA, USA), consistent with the NuGEN manufacturer protocol (See Additional file : Table S1). .. Briefly, 4 ng of total RNA or RiboTag IP RNA were used to synthesize cDNA following the NuGEN’s instructions.

    RNA Sequencing Assay:

    Article Title: A comparative analysis of library prep approaches for sequencing low input translatome samples
    Article Snippet: .. Ovation® RNA-Seq system V2 combined with TruSeq RNA library prep kit v2 We performed a hybrid library preparation by using Ovation® RNA-Seq System V2 (NuGEN, San Carlos, CA, USA) to synthesize cDNA and the TruSeq RNA Library Preparation Kit v2 to construct the sequencing library (Illumina, San Diego, CA, USA), consistent with the NuGEN manufacturer protocol (See Additional file : Table S1). .. Briefly, 4 ng of total RNA or RiboTag IP RNA were used to synthesize cDNA following the NuGEN’s instructions.

    Article Title: A comparative analysis of library prep approaches for sequencing low input translatome samples
    Article Snippet: .. We performed a hybrid library preparation by using Ovation® RNA-Seq System V2 (NuGEN, San Carlos, CA, USA) to synthesize cDNA and the TruSeq RNA Library Preparation Kit v2 to construct the sequencing library (Illumina, San Diego, CA, USA), consistent with the NuGEN manufacturer protocol (See Additional file : Table S1). .. Briefly, 4 ng of total RNA or RiboTag IP RNA were used to synthesize cDNA following the NuGEN’s instructions.

    Article Title: Impact of RNA degradation on fusion detection by RNA-seq
    Article Snippet: .. Popular methods for library preparation in RNA-seq such as Illumina TruSeq® RNA v2 kit use a poly-A pulldown strategy. ..

    Article Title: Depletion of tRNA-halves enables effective small RNA sequencing of low-input murine serum samples
    Article Snippet: .. Small RNA sequencing For Small RNA library preparation we used the TailorMix miRNA sample preparation kit v2 (Seqmatic), the NEBNext Multiplex Small RNA library prep kit (New England Biolabs) or the TruSeq small RNA library preparation kit v2 (Illumina) following manufacturer’s instructions with small modifications ( ). ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Low-cost, low-input RNA-seq protocols perform nearly as well as high-input protocols
    Article Snippet: .. TruSeq libraries were prepared with the “TruSeq RNA Sample Preparation Kit v2” (Illumina Cat.#RS-122-2001) according to manufacturer instructions, except for the following modifications. .. We find that this increases the effective concentration of RNA and cDNA.

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  • 92
    Illumina Inc truseq rna sample prepartaion kit
    Truseq Rna Sample Prepartaion Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq rna sample prepartaion kit/product/Illumina Inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    truseq rna sample prepartaion kit - by Bioz Stars, 2020-09
    92/100 stars
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