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Illumina Inc truseq rna sample preparation kit
Truseq Rna Sample Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 327 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/truseq rna sample preparation kit/product/Illumina Inc
Average 99 stars, based on 327 article reviews
Price from $9.99 to $1999.99
truseq rna sample preparation kit - by Bioz Stars, 2020-02
99/100 stars

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Amplification:

Article Title: RNA-seq analysis reveals alternative splicing under salt stress in cotton, Gossypium davidsonii
Article Snippet: Library preparation for RNA-seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. NRS–122–2002) with 500 ng of total RNA. .. The cDNA libraries were amplified and sequenced using the cBot and HiSeq2000 systems from Illumina.

Article Title: BRD4 promotes p63 and GRHL3 expression downstream of FOXO in mammary epithelial cells
Article Snippet: Briefly, libraries were generated from 500 ng of total RNA using the TruSeq RNA Sample Preparation Kit (Illumina, Cat.No. .. The size range of final cDNA libraries was determined using a DNA 1000 chip on the Bioanalyzer 2100 from Agilent (280 bp). cDNA libraries were amplified and sequenced by using the cBot and HiSeq2000 from Illumina (SR; 1 × 50 bp; 51 cycles with single indexing; 6GB ca.

Article Title: Genetic regulation of salt stress tolerance revealed by RNA-Seq in cotton diploid wild species, Gossypium davidsonii
Article Snippet: Library preparation for RNA-Seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat.NRS–122–2002) with 500 ng of total RNA. .. The cDNA libraries were amplified and sequenced using the cBot and HiSeq2000 systems from Illumina.

Construct:

Article Title: Reduced H3K27me3 leads to abnormal Hox gene expression in neural tube defects
Article Snippet: .. The cDNA library was constructed using a TruSeq RNA Sample Preparation Kit (RS-122-2101, Illumina, USA) according to the manufacturer’s protocols. .. And then, the library could be sequenced using PE91 + 8+91 of Illumina HiSeq™ 2000.

Article Title: Rescue of premature aging defects in Cockayne syndrome stem cells by CRISPR/Cas9-mediated gene correction
Article Snippet: .. After quantification of the RNA by a fragment analyzer (Advanced Analytical), RNA sequencing libraries were constructed using the TruSeq RNA Sample Preparation Kit (Illumina) according to the manufacturer’s protocols. .. Paired-end sequencing was performed using Illumina Hiseq X Ten platform.

Microarray:

Article Title: S100a4 upregulation in Pik3caH1047R;Trp53R270H;MMTV-Cre-driven mammary tumors promotes metastasis
Article Snippet: Data normalization and differential gene expression analysis of the Agilent microarray data was performed using limma [ ]. .. Cell lines developed from the adenocarcinoma were not metastatic in the tail vein injection model. RNA-seq libraries were prepared using the TruSeq RNA Sample Preparation kit (Illumina, CA).

Quantitation Assay:

Article Title: RNA-seq analysis reveals alternative splicing under salt stress in cotton, Gossypium davidsonii
Article Snippet: Library preparation for RNA-seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. NRS–122–2002) with 500 ng of total RNA. .. Accurate quantitation of cDNA libraries was performed using the QuantiFluor dsDNA System (Promega).

Article Title: BRD4 promotes p63 and GRHL3 expression downstream of FOXO in mammary epithelial cells
Article Snippet: Briefly, libraries were generated from 500 ng of total RNA using the TruSeq RNA Sample Preparation Kit (Illumina, Cat.No. .. Accurate quantitation of cDNA libraries was performed using the QuantiFluor™ dsDNA System (Promega, USA).

Article Title: Genetic regulation of salt stress tolerance revealed by RNA-Seq in cotton diploid wild species, Gossypium davidsonii
Article Snippet: Library preparation for RNA-Seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat.NRS–122–2002) with 500 ng of total RNA. .. Accurate quantitation of cDNA libraries was performed using the QuantiFluor dsDNA System (Promega).

Expressing:

Article Title: Epigenetic reprogramming at estrogen-receptor binding sites alters 3D chromatin landscape in endocrine-resistant breast cancer
Article Snippet: The poly(A)-selected RNA libraries were prepared using Illumina TruSeq RNA Sample Preparation kit, spiked-in with ERCC controls and paired-end sequencing was performed using HiSeq2500 instrument. .. Differential gene expression (DEG) analysis was performed using edgeR 3.18.1 , and TMM normalisation was applied to normalise for RNA composition .

Article Title: S100a4 upregulation in Pik3caH1047R;Trp53R270H;MMTV-Cre-driven mammary tumors promotes metastasis
Article Snippet: Paragraph title: Gene expression analysis ... Cell lines developed from the adenocarcinoma were not metastatic in the tail vein injection model. RNA-seq libraries were prepared using the TruSeq RNA Sample Preparation kit (Illumina, CA).

Derivative Assay:

Article Title: 5-hydroxymethylcytosine and gene activity in mouse intestinal differentiation
Article Snippet: Total RNA from four Cd24a_Mid and four Cd24a_Neg samples were submitted for library preparation by the CI-genomics core facility using the TruSeq RNA Sample Preparation Kit (Illumina). .. Gene read counts were then derived using the MMSeq workflow.

Article Title: S100a4 upregulation in Pik3caH1047R;Trp53R270H;MMTV-Cre-driven mammary tumors promotes metastasis
Article Snippet: Metastatic sarcomatoid carcinoma group used in the expression analysis consisted of a Pik3ca H1047R;Trp53 R270H;MMTV-Cre primary tumor (tumor 1), a lung metastatic tumor (tumor 2) derived by tail vein injection involving a cell line derived from tumor 1, a Pik3ca H1047R;MMTV-Cre primary tumor (tumor 3) with a spontaneous Trp53 R245H mutation previously described [ ], a lung metastatic tumor (tumor 4) derived by tail vein injection involving a cell line derived from tumor 3, and a Trp53 R270H;MMTV-Cre primary tumors (tumor 5). .. Cell lines developed from the adenocarcinoma were not metastatic in the tail vein injection model. RNA-seq libraries were prepared using the TruSeq RNA Sample Preparation kit (Illumina, CA).

Flow Cytometry:

Article Title: Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis
Article Snippet: RNA-seq Flow cytometrically sorted naïve CD4 T cells were cultured in Treg cell-inducing conditions as described above. .. At the indicated times post culture, RNA purification was performed using the RNeasy Plus Mini kit (QIAGEN) according to the manufacturer’s instructions. cDNA libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina) following the manufacturer’s protocol.

Ligation:

Article Title: Transcriptome Analysis of the Cecal Tonsil of Jingxing Yellow Chickens Revealed the Mechanism of Differential Resistance to Salmonella
Article Snippet: For the mRNA library construction and deep sequencing, 3 µg total of RNA was prepared using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) to capture the coding transcriptome. .. After cDNA fragments purification and adaptor ligation, sequencing was performed on the HiSeq X Ten platform (Illumina, San Diego, CA, USA).

Cell Culture:

Article Title: Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis
Article Snippet: RNA-seq Flow cytometrically sorted naïve CD4 T cells were cultured in Treg cell-inducing conditions as described above. .. At the indicated times post culture, RNA purification was performed using the RNeasy Plus Mini kit (QIAGEN) according to the manufacturer’s instructions. cDNA libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina) following the manufacturer’s protocol.

Generated:

Article Title: BRD4 promotes p63 and GRHL3 expression downstream of FOXO in mammary epithelial cells
Article Snippet: .. Briefly, libraries were generated from 500 ng of total RNA using the TruSeq RNA Sample Preparation Kit (Illumina, Cat.No. ..

Article Title: Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis
Article Snippet: .. At the indicated times post culture, RNA purification was performed using the RNeasy Plus Mini kit (QIAGEN) according to the manufacturer’s instructions. cDNA libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina) following the manufacturer’s protocol. .. Libraries were sequenced using the Illumina NextSeq 500, producing at least 25 million paired-end 81 bp reads.

Sequencing:

Article Title: Transcriptome Analysis of the Cecal Tonsil of Jingxing Yellow Chickens Revealed the Mechanism of Differential Resistance to Salmonella
Article Snippet: .. For the mRNA library construction and deep sequencing, 3 µg total of RNA was prepared using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) to capture the coding transcriptome. .. After purification, the RNA was fragmented using divalent cations at 95 °C.

Article Title: Reduced H3K27me3 leads to abnormal Hox gene expression in neural tube defects
Article Snippet: Paragraph title: RNA extraction, cDNA library construction and Illumina sequencing ... The cDNA library was constructed using a TruSeq RNA Sample Preparation Kit (RS-122-2101, Illumina, USA) according to the manufacturer’s protocols.

Article Title: Epigenetic reprogramming at estrogen-receptor binding sites alters 3D chromatin landscape in endocrine-resistant breast cancer
Article Snippet: .. The poly(A)-selected RNA libraries were prepared using Illumina TruSeq RNA Sample Preparation kit, spiked-in with ERCC controls and paired-end sequencing was performed using HiSeq2500 instrument. ..

Article Title: Dataset of de novo assembly and functional annotation of the transcriptome of certain developmental stages of coconut rhinoceros beetle, Oryctes rhinoceros L.
Article Snippet: Paragraph title: RNA extraction and sequencing ... As per Hull et al. [ ], total RNA extraction and library generation (TruSeq RNA Sample Preparation Kit v2; Illumina Inc., San Diego, USA) were performed.

Article Title: Rescue of premature aging defects in Cockayne syndrome stem cells by CRISPR/Cas9-mediated gene correction
Article Snippet: After quantification of the RNA by a fragment analyzer (Advanced Analytical), RNA sequencing libraries were constructed using the TruSeq RNA Sample Preparation Kit (Illumina) according to the manufacturer’s protocols. .. Paired-end sequencing was performed using Illumina Hiseq X Ten platform.

Article Title: Transcriptome analysis of the fungal pathogen Rosellinia necatrix during infection of a susceptible avocado rootstock identifies potential mechanisms of pathogenesis
Article Snippet: Paragraph title: RNA isolation and sequencing ... Two μg RNA from each sample were used for RNA library preparation using the TruSeq RNA Sample Preparation Kit (Illumina Inc) according to the protocols recommended by the manufacturer.

Article Title: S100a4 upregulation in Pik3caH1047R;Trp53R270H;MMTV-Cre-driven mammary tumors promotes metastasis
Article Snippet: Cell lines developed from the adenocarcinoma were not metastatic in the tail vein injection model. RNA-seq libraries were prepared using the TruSeq RNA Sample Preparation kit (Illumina, CA). .. All sequencing reads were evaluated for quality using the Bioconductor ShortRead package.

Injection:

Article Title: S100a4 upregulation in Pik3caH1047R;Trp53R270H;MMTV-Cre-driven mammary tumors promotes metastasis
Article Snippet: .. Cell lines developed from the adenocarcinoma were not metastatic in the tail vein injection model. RNA-seq libraries were prepared using the TruSeq RNA Sample Preparation kit (Illumina, CA). ..

Nucleic Acid Electrophoresis:

Article Title: RNA-seq analysis reveals alternative splicing under salt stress in cotton, Gossypium davidsonii
Article Snippet: The RNA was digested with RNase-Free DNase (Qiagen) and checked for integrity by capillary gel electrophoresis. .. Library preparation for RNA-seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. NRS–122–2002) with 500 ng of total RNA.

Article Title: Genetic regulation of salt stress tolerance revealed by RNA-Seq in cotton diploid wild species, Gossypium davidsonii
Article Snippet: The RNA was digested with RNase-Free DNase (Qiagen) and checked for integrity by capillary gel electrophoresis. .. Library preparation for RNA-Seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat.NRS–122–2002) with 500 ng of total RNA.

RNA Sequencing Assay:

Article Title: RNA-seq analysis reveals alternative splicing under salt stress in cotton, Gossypium davidsonii
Article Snippet: .. Library preparation for RNA-seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. NRS–122–2002) with 500 ng of total RNA. .. Accurate quantitation of cDNA libraries was performed using the QuantiFluor dsDNA System (Promega).

Article Title: 5-hydroxymethylcytosine and gene activity in mouse intestinal differentiation
Article Snippet: Paragraph title: RNA-seq ... Total RNA from four Cd24a_Mid and four Cd24a_Neg samples were submitted for library preparation by the CI-genomics core facility using the TruSeq RNA Sample Preparation Kit (Illumina).

Article Title: Epigenetic reprogramming at estrogen-receptor binding sites alters 3D chromatin landscape in endocrine-resistant breast cancer
Article Snippet: Paragraph title: RNA-seq experiments and analysis ... The poly(A)-selected RNA libraries were prepared using Illumina TruSeq RNA Sample Preparation kit, spiked-in with ERCC controls and paired-end sequencing was performed using HiSeq2500 instrument.

Article Title: BRD4 promotes p63 and GRHL3 expression downstream of FOXO in mammary epithelial cells
Article Snippet: Paragraph title: RNA-sequencing (RNA-seq) and data analysis ... Briefly, libraries were generated from 500 ng of total RNA using the TruSeq RNA Sample Preparation Kit (Illumina, Cat.No.

Article Title: Dataset of de novo assembly and functional annotation of the transcriptome of certain developmental stages of coconut rhinoceros beetle, Oryctes rhinoceros L.
Article Snippet: From the various life stages, approximately 5–10 μg of total RNA was used to prepare the RNA-Seq library using TruSeq RNA Sample Prep Kits (Illumina). .. As per Hull et al. [ ], total RNA extraction and library generation (TruSeq RNA Sample Preparation Kit v2; Illumina Inc., San Diego, USA) were performed.

Article Title: Genetic regulation of salt stress tolerance revealed by RNA-Seq in cotton diploid wild species, Gossypium davidsonii
Article Snippet: .. Library preparation for RNA-Seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat.NRS–122–2002) with 500 ng of total RNA. .. Accurate quantitation of cDNA libraries was performed using the QuantiFluor dsDNA System (Promega).

Article Title: Rescue of premature aging defects in Cockayne syndrome stem cells by CRISPR/Cas9-mediated gene correction
Article Snippet: .. After quantification of the RNA by a fragment analyzer (Advanced Analytical), RNA sequencing libraries were constructed using the TruSeq RNA Sample Preparation Kit (Illumina) according to the manufacturer’s protocols. .. Paired-end sequencing was performed using Illumina Hiseq X Ten platform.

Article Title: Transcriptome analysis of the fungal pathogen Rosellinia necatrix during infection of a susceptible avocado rootstock identifies potential mechanisms of pathogenesis
Article Snippet: Two μg RNA from each sample were used for RNA library preparation using the TruSeq RNA Sample Preparation Kit (Illumina Inc) according to the protocols recommended by the manufacturer. .. The RNA-Seq reads from six libraries (three biological replicates per condition) were processed to remove adaptor sequences, empty reads, low-quality sequences with a Phred score lower than 20 and short reads ( < 25 bp).

Article Title: S100a4 upregulation in Pik3caH1047R;Trp53R270H;MMTV-Cre-driven mammary tumors promotes metastasis
Article Snippet: .. Cell lines developed from the adenocarcinoma were not metastatic in the tail vein injection model. RNA-seq libraries were prepared using the TruSeq RNA Sample Preparation kit (Illumina, CA). ..

Article Title: Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis
Article Snippet: Paragraph title: RNA-seq ... At the indicated times post culture, RNA purification was performed using the RNeasy Plus Mini kit (QIAGEN) according to the manufacturer’s instructions. cDNA libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina) following the manufacturer’s protocol.

Mutagenesis:

Article Title: S100a4 upregulation in Pik3caH1047R;Trp53R270H;MMTV-Cre-driven mammary tumors promotes metastasis
Article Snippet: Metastatic sarcomatoid carcinoma group used in the expression analysis consisted of a Pik3ca H1047R;Trp53 R270H;MMTV-Cre primary tumor (tumor 1), a lung metastatic tumor (tumor 2) derived by tail vein injection involving a cell line derived from tumor 1, a Pik3ca H1047R;MMTV-Cre primary tumor (tumor 3) with a spontaneous Trp53 R245H mutation previously described [ ], a lung metastatic tumor (tumor 4) derived by tail vein injection involving a cell line derived from tumor 3, and a Trp53 R270H;MMTV-Cre primary tumors (tumor 5). .. Cell lines developed from the adenocarcinoma were not metastatic in the tail vein injection model. RNA-seq libraries were prepared using the TruSeq RNA Sample Preparation kit (Illumina, CA).

Isolation:

Article Title: Transcriptome Analysis of the Cecal Tonsil of Jingxing Yellow Chickens Revealed the Mechanism of Differential Resistance to Salmonella
Article Snippet: Paragraph title: 2.3. Total RNA Isolation, cDNA Library Construction, and Sequencing ... For the mRNA library construction and deep sequencing, 3 µg total of RNA was prepared using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) to capture the coding transcriptome.

Article Title: Epigenetic reprogramming at estrogen-receptor binding sites alters 3D chromatin landscape in endocrine-resistant breast cancer
Article Snippet: RNA-seq experiments and analysis RNA was isolated from MCF7, TAMR and FASR cells at ~80% confluence using Trizol. .. The poly(A)-selected RNA libraries were prepared using Illumina TruSeq RNA Sample Preparation kit, spiked-in with ERCC controls and paired-end sequencing was performed using HiSeq2500 instrument.

Article Title: Dataset of de novo assembly and functional annotation of the transcriptome of certain developmental stages of coconut rhinoceros beetle, Oryctes rhinoceros L.
Article Snippet: 2.2 RNA extraction and sequencing Total RNA isolated from the whole body tissue of each individual stage was pooled (four samples/stage) and snap-frozen immediately in liquid nitrogen and ground to a fine powder using mortar and pestle. .. As per Hull et al. [ ], total RNA extraction and library generation (TruSeq RNA Sample Preparation Kit v2; Illumina Inc., San Diego, USA) were performed.

Article Title: Transcriptome analysis of the fungal pathogen Rosellinia necatrix during infection of a susceptible avocado rootstock identifies potential mechanisms of pathogenesis
Article Snippet: Paragraph title: RNA isolation and sequencing ... Two μg RNA from each sample were used for RNA library preparation using the TruSeq RNA Sample Preparation Kit (Illumina Inc) according to the protocols recommended by the manufacturer.

Purification:

Article Title: Transcriptome Analysis of the Cecal Tonsil of Jingxing Yellow Chickens Revealed the Mechanism of Differential Resistance to Salmonella
Article Snippet: For the mRNA library construction and deep sequencing, 3 µg total of RNA was prepared using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) to capture the coding transcriptome. .. After purification, the RNA was fragmented using divalent cations at 95 °C.

Article Title: Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis
Article Snippet: .. At the indicated times post culture, RNA purification was performed using the RNeasy Plus Mini kit (QIAGEN) according to the manufacturer’s instructions. cDNA libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina) following the manufacturer’s protocol. .. Libraries were sequenced using the Illumina NextSeq 500, producing at least 25 million paired-end 81 bp reads.

cDNA Library Assay:

Article Title: RNA-seq analysis reveals alternative splicing under salt stress in cotton, Gossypium davidsonii
Article Snippet: Paragraph title: RNA extraction, cDNA library preparation, and RNA-seq ... Library preparation for RNA-seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. NRS–122–2002) with 500 ng of total RNA.

Article Title: Transcriptome Analysis of the Cecal Tonsil of Jingxing Yellow Chickens Revealed the Mechanism of Differential Resistance to Salmonella
Article Snippet: Paragraph title: 2.3. Total RNA Isolation, cDNA Library Construction, and Sequencing ... For the mRNA library construction and deep sequencing, 3 µg total of RNA was prepared using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) to capture the coding transcriptome.

Article Title: Reduced H3K27me3 leads to abnormal Hox gene expression in neural tube defects
Article Snippet: .. The cDNA library was constructed using a TruSeq RNA Sample Preparation Kit (RS-122-2101, Illumina, USA) according to the manufacturer’s protocols. .. And then, the library could be sequenced using PE91 + 8+91 of Illumina HiSeq™ 2000.

Article Title: Genetic regulation of salt stress tolerance revealed by RNA-Seq in cotton diploid wild species, Gossypium davidsonii
Article Snippet: Paragraph title: RNA extraction, cDNA library preparation, and RNA-Seq ... Library preparation for RNA-Seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat.NRS–122–2002) with 500 ng of total RNA.

Sample Prep:

Article Title: RNA-seq analysis reveals alternative splicing under salt stress in cotton, Gossypium davidsonii
Article Snippet: .. Library preparation for RNA-seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. NRS–122–2002) with 500 ng of total RNA. .. Accurate quantitation of cDNA libraries was performed using the QuantiFluor dsDNA System (Promega).

Article Title: 5-hydroxymethylcytosine and gene activity in mouse intestinal differentiation
Article Snippet: .. Total RNA from four Cd24a_Mid and four Cd24a_Neg samples were submitted for library preparation by the CI-genomics core facility using the TruSeq RNA Sample Preparation Kit (Illumina). .. Barcoded samples were sequenced on a single lane of Illumina HiSeq to a depth of more than 200 million paired end (PE) 100-based pair (BP) reads.

Article Title: Transcriptome Analysis of the Cecal Tonsil of Jingxing Yellow Chickens Revealed the Mechanism of Differential Resistance to Salmonella
Article Snippet: .. For the mRNA library construction and deep sequencing, 3 µg total of RNA was prepared using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) to capture the coding transcriptome. .. After purification, the RNA was fragmented using divalent cations at 95 °C.

Article Title: Reduced H3K27me3 leads to abnormal Hox gene expression in neural tube defects
Article Snippet: .. The cDNA library was constructed using a TruSeq RNA Sample Preparation Kit (RS-122-2101, Illumina, USA) according to the manufacturer’s protocols. .. And then, the library could be sequenced using PE91 + 8+91 of Illumina HiSeq™ 2000.

Article Title: Epigenetic reprogramming at estrogen-receptor binding sites alters 3D chromatin landscape in endocrine-resistant breast cancer
Article Snippet: .. The poly(A)-selected RNA libraries were prepared using Illumina TruSeq RNA Sample Preparation kit, spiked-in with ERCC controls and paired-end sequencing was performed using HiSeq2500 instrument. ..

Article Title: BRD4 promotes p63 and GRHL3 expression downstream of FOXO in mammary epithelial cells
Article Snippet: .. Briefly, libraries were generated from 500 ng of total RNA using the TruSeq RNA Sample Preparation Kit (Illumina, Cat.No. ..

Article Title: Dataset of de novo assembly and functional annotation of the transcriptome of certain developmental stages of coconut rhinoceros beetle, Oryctes rhinoceros L.
Article Snippet: .. As per Hull et al. [ ], total RNA extraction and library generation (TruSeq RNA Sample Preparation Kit v2; Illumina Inc., San Diego, USA) were performed. .. All the four samples were sequenced using an Illumina HiSeq2500 in rapid run mode (paired-end 100-bp reads).

Article Title: Genetic regulation of salt stress tolerance revealed by RNA-Seq in cotton diploid wild species, Gossypium davidsonii
Article Snippet: .. Library preparation for RNA-Seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat.NRS–122–2002) with 500 ng of total RNA. .. Accurate quantitation of cDNA libraries was performed using the QuantiFluor dsDNA System (Promega).

Article Title: Rescue of premature aging defects in Cockayne syndrome stem cells by CRISPR/Cas9-mediated gene correction
Article Snippet: .. After quantification of the RNA by a fragment analyzer (Advanced Analytical), RNA sequencing libraries were constructed using the TruSeq RNA Sample Preparation Kit (Illumina) according to the manufacturer’s protocols. .. Paired-end sequencing was performed using Illumina Hiseq X Ten platform.

Article Title: Transcriptome analysis of the fungal pathogen Rosellinia necatrix during infection of a susceptible avocado rootstock identifies potential mechanisms of pathogenesis
Article Snippet: .. Two μg RNA from each sample were used for RNA library preparation using the TruSeq RNA Sample Preparation Kit (Illumina Inc) according to the protocols recommended by the manufacturer. ..

Article Title: S100a4 upregulation in Pik3caH1047R;Trp53R270H;MMTV-Cre-driven mammary tumors promotes metastasis
Article Snippet: .. Cell lines developed from the adenocarcinoma were not metastatic in the tail vein injection model. RNA-seq libraries were prepared using the TruSeq RNA Sample Preparation kit (Illumina, CA). ..

Article Title: Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis
Article Snippet: .. At the indicated times post culture, RNA purification was performed using the RNeasy Plus Mini kit (QIAGEN) according to the manufacturer’s instructions. cDNA libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina) following the manufacturer’s protocol. .. Libraries were sequenced using the Illumina NextSeq 500, producing at least 25 million paired-end 81 bp reads.

Chloramphenicol Acetyltransferase Assay:

Article Title: BRD4 promotes p63 and GRHL3 expression downstream of FOXO in mammary epithelial cells
Article Snippet: .. Briefly, libraries were generated from 500 ng of total RNA using the TruSeq RNA Sample Preparation Kit (Illumina, Cat.No. ..

Article Title: Genetic regulation of salt stress tolerance revealed by RNA-Seq in cotton diploid wild species, Gossypium davidsonii
Article Snippet: .. Library preparation for RNA-Seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat.NRS–122–2002) with 500 ng of total RNA. .. Accurate quantitation of cDNA libraries was performed using the QuantiFluor dsDNA System (Promega).

Mouse Assay:

Article Title: Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis
Article Snippet: At the indicated times post culture, RNA purification was performed using the RNeasy Plus Mini kit (QIAGEN) according to the manufacturer’s instructions. cDNA libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina) following the manufacturer’s protocol. .. In another experiment, total Treg cells were flow cytometrically sorted from the spleens of Bach2 fl/fl Foxp3 Cre and Foxp3 Cre control mice and subjected to RNA-seq.

Chromatin Immunoprecipitation:

Article Title: RNA-seq analysis reveals alternative splicing under salt stress in cotton, Gossypium davidsonii
Article Snippet: Library preparation for RNA-seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. NRS–122–2002) with 500 ng of total RNA. .. The size range of the final cDNA libraries was determined by applying the DNA 1000 chip on the Bioanalyzer 2100 (Agilent; 280 bp).

Article Title: BRD4 promotes p63 and GRHL3 expression downstream of FOXO in mammary epithelial cells
Article Snippet: Briefly, libraries were generated from 500 ng of total RNA using the TruSeq RNA Sample Preparation Kit (Illumina, Cat.No. .. The size range of final cDNA libraries was determined using a DNA 1000 chip on the Bioanalyzer 2100 from Agilent (280 bp). cDNA libraries were amplified and sequenced by using the cBot and HiSeq2000 from Illumina (SR; 1 × 50 bp; 51 cycles with single indexing; 6GB ca.

Article Title: Dataset of de novo assembly and functional annotation of the transcriptome of certain developmental stages of coconut rhinoceros beetle, Oryctes rhinoceros L.
Article Snippet: The quality and the purity of the extracted RNA were assessed by OD 260/280 ratio and RNA integrity number was analyzed using an Agilent Technologies 2100 Bioanalyzer with the Agilent RNA chip with RIN (RNA Integrity Number) > 8.0. .. As per Hull et al. [ ], total RNA extraction and library generation (TruSeq RNA Sample Preparation Kit v2; Illumina Inc., San Diego, USA) were performed.

Article Title: Genetic regulation of salt stress tolerance revealed by RNA-Seq in cotton diploid wild species, Gossypium davidsonii
Article Snippet: Library preparation for RNA-Seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat.NRS–122–2002) with 500 ng of total RNA. .. The size range of the final cDNA libraries was determined by applying the DNA 1000 chip on the Bioanalyzer 2100 (Agilent; 280 bp).

RNA Extraction:

Article Title: RNA-seq analysis reveals alternative splicing under salt stress in cotton, Gossypium davidsonii
Article Snippet: Paragraph title: RNA extraction, cDNA library preparation, and RNA-seq ... Library preparation for RNA-seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. NRS–122–2002) with 500 ng of total RNA.

Article Title: Reduced H3K27me3 leads to abnormal Hox gene expression in neural tube defects
Article Snippet: Paragraph title: RNA extraction, cDNA library construction and Illumina sequencing ... The cDNA library was constructed using a TruSeq RNA Sample Preparation Kit (RS-122-2101, Illumina, USA) according to the manufacturer’s protocols.

Article Title: Dataset of de novo assembly and functional annotation of the transcriptome of certain developmental stages of coconut rhinoceros beetle, Oryctes rhinoceros L.
Article Snippet: .. As per Hull et al. [ ], total RNA extraction and library generation (TruSeq RNA Sample Preparation Kit v2; Illumina Inc., San Diego, USA) were performed. .. All the four samples were sequenced using an Illumina HiSeq2500 in rapid run mode (paired-end 100-bp reads).

Article Title: Genetic regulation of salt stress tolerance revealed by RNA-Seq in cotton diploid wild species, Gossypium davidsonii
Article Snippet: Paragraph title: RNA extraction, cDNA library preparation, and RNA-Seq ... Library preparation for RNA-Seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat.NRS–122–2002) with 500 ng of total RNA.

Agarose Gel Electrophoresis:

Article Title: Transcriptome analysis of the fungal pathogen Rosellinia necatrix during infection of a susceptible avocado rootstock identifies potential mechanisms of pathogenesis
Article Snippet: RNA integrity was confirmed by the appearance of ribosomal RNA bands and lack of degradation products after separation on a 2% agarose gel and Red Safe staining. .. Two μg RNA from each sample were used for RNA library preparation using the TruSeq RNA Sample Preparation Kit (Illumina Inc) according to the protocols recommended by the manufacturer.

Spectrophotometry:

Article Title: Transcriptome analysis of the fungal pathogen Rosellinia necatrix during infection of a susceptible avocado rootstock identifies potential mechanisms of pathogenesis
Article Snippet: RNA quantity and quality were determined based on absorbance ratios at 260 nm/280 nm and 260 nm/230 nm using a NanoDrop® ND-1000 (Nanodrop Technologies, Inc., Montchanin, USA) spectrophotometer. .. Two μg RNA from each sample were used for RNA library preparation using the TruSeq RNA Sample Preparation Kit (Illumina Inc) according to the protocols recommended by the manufacturer.

Concentration Assay:

Article Title: Reduced H3K27me3 leads to abnormal Hox gene expression in neural tube defects
Article Snippet: RNA concentration and quality were assessed by Agilent 2100 Bioanalyzer. .. The cDNA library was constructed using a TruSeq RNA Sample Preparation Kit (RS-122-2101, Illumina, USA) according to the manufacturer’s protocols.

High Throughput Screening Assay:

Article Title: BRD4 promotes p63 and GRHL3 expression downstream of FOXO in mammary epithelial cells
Article Snippet: RNA-sequencing (RNA-seq) and data analysis The high-throughput RNA-seq library preparation and data analysis were performed as described ( ). .. Briefly, libraries were generated from 500 ng of total RNA using the TruSeq RNA Sample Preparation Kit (Illumina, Cat.No.

Staining:

Article Title: Transcriptome analysis of the fungal pathogen Rosellinia necatrix during infection of a susceptible avocado rootstock identifies potential mechanisms of pathogenesis
Article Snippet: RNA integrity was confirmed by the appearance of ribosomal RNA bands and lack of degradation products after separation on a 2% agarose gel and Red Safe staining. .. Two μg RNA from each sample were used for RNA library preparation using the TruSeq RNA Sample Preparation Kit (Illumina Inc) according to the protocols recommended by the manufacturer.

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    Illumina Inc truseq rna sample prepartaion kit
    Truseq Rna Sample Prepartaion Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq rna sample prepartaion kit/product/Illumina Inc
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    truseq rna sample prepartaion kit - by Bioz Stars, 2020-02
    84/100 stars
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