truseq custom amplicon low input tsca li library prep kit  (Illumina Inc)

 
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    Name:
    TruSeq Custom Amplicon Kit Dx
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    Catalog Number:
    20005718
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    Category:
    In Vitro Diagnostic IVD Products
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    Illumina Inc truseq custom amplicon low input tsca li library prep kit
    TruSeq Custom Amplicon Kit Dx

    https://www.bioz.com/result/truseq custom amplicon low input tsca li library prep kit/product/Illumina Inc
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    truseq custom amplicon low input tsca li library prep kit - by Bioz Stars, 2021-01
    98/100 stars

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    Related Articles

    Sequencing:

    Article Title: Biased estimates of clonal evolution and subclonal heterogeneity can arise from PCR duplicates in deep sequencing experiments
    Article Snippet: .. Adapting Illumina TruSeq to use single molecule tagging The Illumina TruSeq Custom Amplicon Kit is a multiplex system for targeted sequencing that allows for approximately 1,500 amplicons to be sequenced at the same time. .. Custom probes with sequence flanking the target region are generated and, during sample preparation, the region is extended from one probe and then ligated to the second probe (Figure A).

    Article Title: Biased estimates of clonal evolution and subclonal heterogeneity can arise from PCR duplicates in deep sequencing experiments
    Article Snippet: .. Targeted resequencing of somatic variantsWe performed deep-targeted sequencing using the Illumina TruSeq Custom Amplicon kit. .. Using DesignStudio software, probes were successfully designed to cover approximately 1,000 somatic variants identified in an exome sequencing project of 18 CLL patients to study clonal evolution (in preparation) and about 200 common SNPs, resulting in 1,225 targets of around 150 bp in length.

    Article Title: Validation and Implementation of a Custom Next-Generation Sequencing Clinical Assay for Hematologic Malignancies
    Article Snippet: .. The assay relies on an Illumina Truseq Custom Amplicon (TSCA) (San Diego, CA) kit and identifies single nucleotide variants (SNVs) and insertions/deletions (indels) in genes that are recurrently mutated in myeloid disorders and sequence variants in certain genes that are commonly mutated in lymphoid leukemias, such as NOTCH1 , NOTCH2, STAT3 , and MYD88 ., , , Genes covered by the assay encode a diverse collection of transcription factors, epigenetic regulators, cohesin family members, splicing factors, cell surface receptors, and downstream signaling components. .. In this report, we describe the design, analysis pipeline, validation, strategy for annotation, and reporting of this NGS panel [referred to as rapid heme panel (RHP)] and present the comparison of results from this amplicon-based panel with results obtained with a capture-based NGS panel.

    Polymerase Chain Reaction:

    Article Title: Biased estimates of clonal evolution and subclonal heterogeneity can arise from PCR duplicates in deep sequencing experiments
    Article Snippet: .. This indicates that our method effectively incorporates diverse SMT oligomers into the library preparation process and suggests that the Illumina TruSeq Custom Amplicon Kit generates a moderate number of PCR duplicate reads that are evenly distributed across targeted sequences. .. Single molecule tag-identified duplicates are PCR duplicates While the expected complexity of a 12 bp random oligomer is high (412 combinations), the same oligomer could be incorporated multiple times by chance if there are biases in how the oligomer was generated or incorporated.

    Article Title: Biased estimates of clonal evolution and subclonal heterogeneity can arise from PCR duplicates in deep sequencing experiments
    Article Snippet: .. Here we adapted the Illumina TruSeq Custom Amplicon kit to include single molecule tagging (SMT) and show that SMT-identified duplicates arise from PCR. .. We demonstrate that retention of PCR duplicate reads can imply clonal evolution when none exists, while their removal effectively controls the false positive rate.

    Multiplex Assay:

    Article Title: Biased estimates of clonal evolution and subclonal heterogeneity can arise from PCR duplicates in deep sequencing experiments
    Article Snippet: .. Adapting Illumina TruSeq to use single molecule tagging The Illumina TruSeq Custom Amplicon Kit is a multiplex system for targeted sequencing that allows for approximately 1,500 amplicons to be sequenced at the same time. .. Custom probes with sequence flanking the target region are generated and, during sample preparation, the region is extended from one probe and then ligated to the second probe (Figure A).

    Amplification:

    Article Title: Biased estimates of clonal evolution and subclonal heterogeneity can arise from PCR duplicates in deep sequencing experiments
    Article Snippet: .. Adapting Illumina TruSeq to use single molecule tagging The Illumina TruSeq Custom Amplicon Kit is a multiplex system for targeted sequencing that allows for approximately 1,500 amplicons to be sequenced at the same time. .. Custom probes with sequence flanking the target region are generated and, during sample preparation, the region is extended from one probe and then ligated to the second probe (Figure A).

    Article Title: Evaluation of Mannose Binding Lectin Gene Variants in Pediatric Influenza Virus-Related Critical Illness
    Article Snippet: .. To identify rare deleterious variants in MBL2 , we performed targeted re-sequencing of the exons and nearby regions using Illumina TruSeq Custom Amplicon kit (TSCA, Illumina, San Diego, CA) as described previously , and this was compared with Taqman genotyping. .. In samples where genotyping remained inconclusive, Sanger sequencing for the 5 SNPs in MBL2 was performed.

    Article Title: Biased estimates of clonal evolution and subclonal heterogeneity can arise from PCR duplicates in deep sequencing experiments
    Article Snippet: .. This indicates that our method effectively incorporates diverse SMT oligomers into the library preparation process and suggests that the Illumina TruSeq Custom Amplicon Kit generates a moderate number of PCR duplicate reads that are evenly distributed across targeted sequences. .. Single molecule tag-identified duplicates are PCR duplicates While the expected complexity of a 12 bp random oligomer is high (412 combinations), the same oligomer could be incorporated multiple times by chance if there are biases in how the oligomer was generated or incorporated.

    Article Title: Biased estimates of clonal evolution and subclonal heterogeneity can arise from PCR duplicates in deep sequencing experiments
    Article Snippet: .. Targeted resequencing of somatic variantsWe performed deep-targeted sequencing using the Illumina TruSeq Custom Amplicon kit. .. Using DesignStudio software, probes were successfully designed to cover approximately 1,000 somatic variants identified in an exome sequencing project of 18 CLL patients to study clonal evolution (in preparation) and about 200 common SNPs, resulting in 1,225 targets of around 150 bp in length.

    Article Title: Somatic Mutations in Cerebral Cortical Malformations
    Article Snippet: .. We used the Illumina TruSeq Custom Amplicon Kit to capture all exons, intron–exon boundaries, and 10-bp flanking sequences of target genes (RefSeq database, hg19 assembly). ..

    Article Title: Biased estimates of clonal evolution and subclonal heterogeneity can arise from PCR duplicates in deep sequencing experiments
    Article Snippet: .. Here we adapted the Illumina TruSeq Custom Amplicon kit to include single molecule tagging (SMT) and show that SMT-identified duplicates arise from PCR. .. We demonstrate that retention of PCR duplicate reads can imply clonal evolution when none exists, while their removal effectively controls the false positive rate.

    Article Title: High NPM1-mutant allele burden at diagnosis predicts unfavorable outcomes in de novo AML
    Article Snippet: .. - Target regions of 87 genes (hybrid capture system; Agilent Technologies, Santa Clara, CA) were evaluated in 46 patients, 95 genes (rapid heme panel [RHP]; Illumina TruSeq custom amplicon kit; San Diego, CA) in 56 patients, and 54 genes (Massachusetts General Hospital; SNaPshot; Illumina TruSeq) in 7 patients; genes were selected based on pathogenic involvement in myeloid malignancies. ..

    Article Title: Validation and Implementation of a Custom Next-Generation Sequencing Clinical Assay for Hematologic Malignancies
    Article Snippet: .. The assay relies on an Illumina Truseq Custom Amplicon (TSCA) (San Diego, CA) kit and identifies single nucleotide variants (SNVs) and insertions/deletions (indels) in genes that are recurrently mutated in myeloid disorders and sequence variants in certain genes that are commonly mutated in lymphoid leukemias, such as NOTCH1 , NOTCH2, STAT3 , and MYD88 ., , , Genes covered by the assay encode a diverse collection of transcription factors, epigenetic regulators, cohesin family members, splicing factors, cell surface receptors, and downstream signaling components. .. In this report, we describe the design, analysis pipeline, validation, strategy for annotation, and reporting of this NGS panel [referred to as rapid heme panel (RHP)] and present the comparison of results from this amplicon-based panel with results obtained with a capture-based NGS panel.

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    Illumina Inc truseq custom amplicon low input tsca li library prep kit
    Truseq Custom Amplicon Low Input Tsca Li Library Prep Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq custom amplicon low input tsca li library prep kit/product/Illumina Inc
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    truseq custom amplicon low input tsca li library prep kit - by Bioz Stars, 2021-01
    98/100 stars
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