truseq custom amplicon low input kit  (Illumina Inc)

 
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    Name:
    TruSeq Custom Amplicon Index Kit
    Description:
    The TruSight Myeloid Sequencing Panel uses expert defined content and proven next generation sequencing NGS technology to identify somatic variants in myeloid malignancies The panel content was designed by a consortium of recognized experts in blood cancer disorders and targets genes frequently mutated in Acute myeloid leukemia AML Myelodysplastic syndrome MDS Myeloproliferative neoplasms MPN Chronic myelogenous leukemia CML Chronic myelomonocytic leukemia CMML Juvenile myelomonocytic leukemia JMML The TruSight Myeloid Sequencing Panel covers 15 full genes exons only and 39 additional genes where oncogenic hotspots are covered providing a comprehensive assessment of the key genes known to be involved in myeloid malignancies in a single test The result is an accurate cost effective solution that enables researchers to profile liquid tumors View the gene list TruSight Myeloid libraries are ideally suited to run on a desktop sequencer and perform alignment and variant calling with the MiSeq Reporter or Local Run Manager Amplicon workflow with Somatic Variant Caller Filtering and annotation can then be performed using BaseSpace Variant Interpreter See All Illumina Cancer Research Panels
    Catalog Number:
    fc-130-1003
    Price:
    None
    Category:
    Clinical Research Products
    Buy from Supplier


    Structured Review

    Illumina Inc truseq custom amplicon low input kit
    TruSeq Custom Amplicon Index Kit
    The TruSight Myeloid Sequencing Panel uses expert defined content and proven next generation sequencing NGS technology to identify somatic variants in myeloid malignancies The panel content was designed by a consortium of recognized experts in blood cancer disorders and targets genes frequently mutated in Acute myeloid leukemia AML Myelodysplastic syndrome MDS Myeloproliferative neoplasms MPN Chronic myelogenous leukemia CML Chronic myelomonocytic leukemia CMML Juvenile myelomonocytic leukemia JMML The TruSight Myeloid Sequencing Panel covers 15 full genes exons only and 39 additional genes where oncogenic hotspots are covered providing a comprehensive assessment of the key genes known to be involved in myeloid malignancies in a single test The result is an accurate cost effective solution that enables researchers to profile liquid tumors View the gene list TruSight Myeloid libraries are ideally suited to run on a desktop sequencer and perform alignment and variant calling with the MiSeq Reporter or Local Run Manager Amplicon workflow with Somatic Variant Caller Filtering and annotation can then be performed using BaseSpace Variant Interpreter See All Illumina Cancer Research Panels
    https://www.bioz.com/result/truseq custom amplicon low input kit/product/Illumina Inc
    Average 96 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    truseq custom amplicon low input kit - by Bioz Stars, 2020-07
    96/100 stars

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    Related Articles

    Amplification:

    Article Title: NPM1 mutated AML can relapse with wild-type NPM1: persistent clonal hematopoiesis can drive relapse
    Article Snippet: .. The library of 63 genes was generated with a TruSeq Custom Amplicon Low Input Kit (Illumina, San Diego, CA) following the manufacturer’s protocol. ..

    Article Title: Development and analytical validation of a next-generation sequencing based microsatellite instability (MSI) assay
    Article Snippet: .. Library preparation and NGS MSI NGS libraries were prepared from 20 ng DNA using the TruSeq Custom Amplicon Low Input Kit (Illumina, San Diego, CA, USA). ..

    Article Title: The most 5′ truncating homozygous mutation of WNT1 in siblings with osteogenesis imperfecta with a variable degree of brain anomalies: a case report
    Article Snippet: .. Sixteen known OI genes, BMP1 , COL1A1 , COL1A2 , CREB3L1 , CRTAP , FKBP10 , IFITM5 , LEPRE1 , PLOD2 , PPIB , SERPINF1 , SERPINH1 , SP7 , TMEM38B , WNT1 , and MBTPS2 , were amplified from 200 ng of genomic DNA using the Truseq Custom Amplicon Sequencing kit (Illumina, San Diego, CA). .. 286 amplicons which covered all the 226 exons (28 kb) of the target genes were sequenced by Miseq (Illumina, San Diego, CA) using 2 × 250 paired-end reads.

    Article Title: Targeted next generation sequencing of RB1 gene for the molecular diagnosis of Retinoblastoma
    Article Snippet: .. A Primer library was custom-designed to amplify 27 exons, exon/intron boundaries and promoter region of RB1 gene using the Illumina Truseq custom Amplicon and Agilent SureSelect in-solution hybridization capture kits by the service provider (Scigenom, Kochi, India). ..

    Article Title: Maternal Sensitivity Buffers the Association between SLC6A4 Methylation and Socio-Emotional Stress Response in 3-Month-Old Full Term, but not very Preterm Infants
    Article Snippet: .. Secondary PCR was conducted on each sample using a TruSeq Custom Amplicon Index Kit (Illumina) containing eight forward (i5) and 12 reverse (i7) index primers. .. Optimal annealing temperature (68°C) and number of PCR cycles were experimentally determined.

    Article Title: Molecular characterization of AML with RUNX1-RUNX1T1 at diagnosis and relapse reveals net loss of co-mutations
    Article Snippet: .. The library of 63 genes was generated with a TruSeq Custom Amplicon Low Input Kit (Illumina, San Diego, CA), following the manufacturers’ protocol. ..

    Article Title: Paradoxical psoriasis induced by TNF‐α blockade shows immunological features typical of the early phase of psoriasis development
    Article Snippet: .. The SNP panel was analyzed by targeted sequencing, using NGS TruSeq Custom Amplicon kit and the MiSeq platform (Illumina, San Diego, CA, USA). ..

    Article Title: Detection and characterization of homozygosity of mutated CALR by copy neutral loss of heterozygosity in myeloproliferative neoplasms among cases with high CALR mutation loads or with progressive disease
    Article Snippet: .. The template library was generated with the TruSeq Custom Amplicon Low Input Kit and sequenced with the NextSeq (Illumina, San Diego, CA, USA; sensitivity: 3%). .. Next-generation sequencing (NGS) data were analyzed using the Sequence Pilot (v.4.1.1 Build 510 for the Illumina platform, JSI Medicalsystems, Kippenheim, Germany).

    Next-Generation Sequencing:

    Article Title: Development and analytical validation of a next-generation sequencing based microsatellite instability (MSI) assay
    Article Snippet: .. Library preparation and NGS MSI NGS libraries were prepared from 20 ng DNA using the TruSeq Custom Amplicon Low Input Kit (Illumina, San Diego, CA, USA). ..

    Article Title: Paradoxical psoriasis induced by TNF‐α blockade shows immunological features typical of the early phase of psoriasis development
    Article Snippet: .. The SNP panel was analyzed by targeted sequencing, using NGS TruSeq Custom Amplicon kit and the MiSeq platform (Illumina, San Diego, CA, USA). ..

    Sequencing:

    Article Title: The most 5′ truncating homozygous mutation of WNT1 in siblings with osteogenesis imperfecta with a variable degree of brain anomalies: a case report
    Article Snippet: .. Sixteen known OI genes, BMP1 , COL1A1 , COL1A2 , CREB3L1 , CRTAP , FKBP10 , IFITM5 , LEPRE1 , PLOD2 , PPIB , SERPINF1 , SERPINH1 , SP7 , TMEM38B , WNT1 , and MBTPS2 , were amplified from 200 ng of genomic DNA using the Truseq Custom Amplicon Sequencing kit (Illumina, San Diego, CA). .. 286 amplicons which covered all the 226 exons (28 kb) of the target genes were sequenced by Miseq (Illumina, San Diego, CA) using 2 × 250 paired-end reads.

    Article Title: Paradoxical psoriasis induced by TNF‐α blockade shows immunological features typical of the early phase of psoriasis development
    Article Snippet: .. The SNP panel was analyzed by targeted sequencing, using NGS TruSeq Custom Amplicon kit and the MiSeq platform (Illumina, San Diego, CA, USA). ..

    Generated:

    Article Title: NPM1 mutated AML can relapse with wild-type NPM1: persistent clonal hematopoiesis can drive relapse
    Article Snippet: .. The library of 63 genes was generated with a TruSeq Custom Amplicon Low Input Kit (Illumina, San Diego, CA) following the manufacturer’s protocol. ..

    Article Title: Molecular characterization of AML with RUNX1-RUNX1T1 at diagnosis and relapse reveals net loss of co-mutations
    Article Snippet: .. The library of 63 genes was generated with a TruSeq Custom Amplicon Low Input Kit (Illumina, San Diego, CA), following the manufacturers’ protocol. ..

    Article Title: Detection and characterization of homozygosity of mutated CALR by copy neutral loss of heterozygosity in myeloproliferative neoplasms among cases with high CALR mutation loads or with progressive disease
    Article Snippet: .. The template library was generated with the TruSeq Custom Amplicon Low Input Kit and sequenced with the NextSeq (Illumina, San Diego, CA, USA; sensitivity: 3%). .. Next-generation sequencing (NGS) data were analyzed using the Sequence Pilot (v.4.1.1 Build 510 for the Illumina platform, JSI Medicalsystems, Kippenheim, Germany).

    Polymerase Chain Reaction:

    Article Title: Maternal Sensitivity Buffers the Association between SLC6A4 Methylation and Socio-Emotional Stress Response in 3-Month-Old Full Term, but not very Preterm Infants
    Article Snippet: .. Secondary PCR was conducted on each sample using a TruSeq Custom Amplicon Index Kit (Illumina) containing eight forward (i5) and 12 reverse (i7) index primers. .. Optimal annealing temperature (68°C) and number of PCR cycles were experimentally determined.

    Hybridization:

    Article Title: Targeted next generation sequencing of RB1 gene for the molecular diagnosis of Retinoblastoma
    Article Snippet: .. A Primer library was custom-designed to amplify 27 exons, exon/intron boundaries and promoter region of RB1 gene using the Illumina Truseq custom Amplicon and Agilent SureSelect in-solution hybridization capture kits by the service provider (Scigenom, Kochi, India). ..

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  • About
  • News
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  • Team
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  • Contact
  • Bioz Stars
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  • 96
    Illumina Inc truseq custom amplicon low input kit
    Truseq Custom Amplicon Low Input Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq custom amplicon low input kit/product/Illumina Inc
    Average 96 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    truseq custom amplicon low input kit - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

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