truchip chromatin shearing kit  (Covaris)


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    Name:
    truChIP Chromatin Shearing Tissue Kit
    Description:
    The truChIP Chromatin Shearing Tissue Kit with Formaldehyde is designed and optimized for the efficient and reproducible shearing of chromatin from tissues 20 120mg using the Covaris Adaptive Focused Acoustics AFA technology
    Catalog Number:
    520237
    Price:
    None
    Applications:
    Chromatin shearing from tissue
    Category:
    Reagent
    Quantity:
    1 0
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    Structured Review

    Covaris truchip chromatin shearing kit
    truChIP Chromatin Shearing Tissue Kit
    The truChIP Chromatin Shearing Tissue Kit with Formaldehyde is designed and optimized for the efficient and reproducible shearing of chromatin from tissues 20 120mg using the Covaris Adaptive Focused Acoustics AFA technology
    https://www.bioz.com/result/truchip chromatin shearing kit/product/Covaris
    Average 95 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    truchip chromatin shearing kit - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: The Dynamic Epigenetic Landscape of the Retina During Development, Reprogramming, and Tumorigenesis
    Article Snippet: The nuclei were isolated and prepared for shearing using TruChIP chromatin shearing kit (Covaris #520127) according to manufacturer’s protocol. .. After de-crosslinking, DNA was extracted using MinElute PCR-purification kit (QIAGEN #28006), quantified using the Quant-iT PicoGreen ds DNA assay (Life Technologies #Q33120) and subjected to qPCR analysis and library construction for sequencing.

    Article Title: Protein arginine methyltransferase 5 promotes bladder cancer growth through inhibiting NF-kB dependent apoptosis
    Article Snippet: The truChIP Chromatin Shearing Kit (Covaris) was used to prepare chromatin. .. The IgG and NF-kB p65 antibodies (2A12A7, Thermo Fisher) were used for immunoprecipitation by the Quick Chip Kit (Imgenex SYBR Green Master Mix, (Appliedbiosystems) was used for qPCR to quantify precipitated DNA).

    Article Title: Epigenetic mechanisms underlying NMDA receptor hypofunction in the prefrontal cortex of juvenile animals in the MAM model for schizophrenia
    Article Snippet: mPFC tissue was collected from saline and MAM animals at P21 and pooled for the chromatin immunoprecipitation (ChIP) assays performed using the truChIP™ Chromatin Shearing Kit (Covaris, PN 520083). .. The eluted material was purified using a QIAquick PCR kit (Qiagen; 28104) and was directly used for qPCR.

    Article Title: Protein arginine methyltransferase 5 promotes bladder cancer growth through inhibiting NF-kB dependent apoptosis
    Article Snippet: Chromatin immunoprecipitation (ChIP) The truChIP Chromatin Shearing Kit (Covaris) was used to prepare chromatin. .. The IgG and NF-kB p65 antibodies (2A12A7, Thermo Fisher) were used for immunoprecipitation by the Quick Chip Kit (Imgenex SYBR Green Master Mix, (Appliedbiosystems) was used for qPCR to quantify precipitated DNA).

    Centrifugation:

    Article Title: Intestinal, but not hepatic, ChREBP is required for fructose tolerance
    Article Snippet: Chromatin was isolated using a truChIP Chromatin Shearing Tissue Kit (Covaris) according to the manufacturer’s protocol, with modifications. .. Cross-linked tissue was dounce homogenized in Lysis Buffer with protease inhibitors, incubated for 20 minutes at 4°C with periodic vortexing, and crude nuclei were collected by centrifugation at 1,700 g and washed in Wash Buffer twice.

    Article Title: Biglycan- and Sphingosine Kinase-1 Signaling Crosstalk Regulates the Synthesis of Macrophage Chemoattractants
    Article Snippet: Chromatin Immunoprecipitation Preparation of cell extracts, crosslinking and isolation of nuclei was performed with the truCHIP™ Chromatin Shearing Kit (Covaris, Woburn, MA, USA) according to the manufacturers protocol. .. After sonification of the lysates with the Bioruptur Plus (10 cycles, 30 s on, 90 s off, 4 °C; Diagenode, Seraing, Belgium), cell debris was removed by centrifugation and the lysates were diluted 1:3 in dilution buffer (20 mmol/L Tris/HCl pH 7.4, 100 mmol/L NaCl, 2 mmol/L ethylenediaminetetraacetic acid (EDTA), 0.5% Triton X-100 and protease inhibitors).

    Article Title: Distinct Requirements of CHD4 during B Cell Development and Antibody Response
    Article Snippet: Cross-linked cells were washed with PBS-based Cell Wash Buffer (PBS, 0.1% FBS, 2mM EDTA) and lysed according to the protocol of truChIP Chromatin Shearing Kit with Formaldehyde (Covaris, 520154). .. Cell lysates were sonicated in the M220 Focused-ultrasonicator (Covaris, 5002295) to shear the chromatin, and debris was removed by centrifugation.

    Agarose Gel Electrophoresis:

    Article Title: IRF5 regulates unique subset of genes in dendritic cells during West Nile virus infection
    Article Snippet: Chromatin was isolated using a truChIP chromatin shearing kit (Covaris) and sheared using M220 focused-ultrasonicator (Covaris). .. Sheared chromatin was run on an agarose gel and band corresponding to size 200–700bp was extracted.

    Sonication:

    Article Title: Single-Cell RNA-Seq Reveals TOX as a Key Regulator of CD8+ T cell Persistence in Chronic Infection
    Article Snippet: .. DNA-protein crosslinking, nuclei isolation, and chromatin sonication were performed using truChIP Chromatin Shearing Kit (Covaris) according to manufacturer’s instructions. .. After immunoprecipitation by anti-H3K27Ac (ab4729, AbCam), ChIPmentation was performed according to the published protocol .

    Article Title: Distinct Requirements of CHD4 during B Cell Development and Antibody Response
    Article Snippet: Cross-linked cells were washed with PBS-based Cell Wash Buffer (PBS, 0.1% FBS, 2mM EDTA) and lysed according to the protocol of truChIP Chromatin Shearing Kit with Formaldehyde (Covaris, 520154). .. Cell lysates were sonicated in the M220 Focused-ultrasonicator (Covaris, 5002295) to shear the chromatin, and debris was removed by centrifugation.

    Article Title: Zc3h10 Acts as a Transcription Factor and Is Phosphorylated to Activate the Thermogenic Program
    Article Snippet: Crosslinked was performed using 1% methanol-free formaldehyde in crosslinking buffer from the truChIP chromatin shearing kit (Covaris) for 5 min. .. Nuclei were sonicated on an M220 for 8mins at the manufacturer recommended power settings.

    Magnetic Beads:

    Article Title: Biglycan- and Sphingosine Kinase-1 Signaling Crosstalk Regulates the Synthesis of Macrophage Chemoattractants
    Article Snippet: Chromatin Immunoprecipitation Preparation of cell extracts, crosslinking and isolation of nuclei was performed with the truCHIP™ Chromatin Shearing Kit (Covaris, Woburn, MA, USA) according to the manufacturers protocol. .. Pre-clearing was done with 20 µL DiaMag protein A coated magnetic beads slurry (Diagenode) for 45 min at 4 °C.

    Neutralization:

    Article Title: Zc3h10 Acts as a Transcription Factor and Is Phosphorylated to Activate the Thermogenic Program
    Article Snippet: Crosslinked was performed using 1% methanol-free formaldehyde in crosslinking buffer from the truChIP chromatin shearing kit (Covaris) for 5 min. .. The manufacturer recommended protocol was followed with the exception of adding 2 dounce steps following the neutralization of the crosslinking with the loose pestle and after addition of the lysis buffer with the tight pestle.

    Transfection:

    Article Title: T Cell Receptor-induced Nuclear Factor κB (NF-κB) Signaling and Transcriptional Activation Are Regulated by STIM1- and Orai1-mediated Calcium Entry *
    Article Snippet: Jurkat T cells (10 × 106 ) transfected with either EGFP-shPKCα or control EGFP-pCMS2 vector (48 h) were stimulated with PMA (200 n m ) and ionomycin (1 μ m ) for 30 min at 37 °C. .. Chromatin was prepared using a Covaris truChIP chromatin shearing kit (Covaris Inc., Woburn, MA).

    Purification:

    Article Title: Epigenetic mechanisms underlying NMDA receptor hypofunction in the prefrontal cortex of juvenile animals in the MAM model for schizophrenia
    Article Snippet: mPFC tissue was collected from saline and MAM animals at P21 and pooled for the chromatin immunoprecipitation (ChIP) assays performed using the truChIP™ Chromatin Shearing Kit (Covaris, PN 520083). .. The eluted material was purified using a QIAquick PCR kit (Qiagen; 28104) and was directly used for qPCR.

    SYBR Green Assay:

    Article Title: Protein arginine methyltransferase 5 promotes bladder cancer growth through inhibiting NF-kB dependent apoptosis
    Article Snippet: The truChIP Chromatin Shearing Kit (Covaris) was used to prepare chromatin. .. The IgG and NF-kB p65 antibodies (2A12A7, Thermo Fisher) were used for immunoprecipitation by the Quick Chip Kit (Imgenex SYBR Green Master Mix, (Appliedbiosystems) was used for qPCR to quantify precipitated DNA).

    Article Title: Protein arginine methyltransferase 5 promotes bladder cancer growth through inhibiting NF-kB dependent apoptosis
    Article Snippet: Chromatin immunoprecipitation (ChIP) The truChIP Chromatin Shearing Kit (Covaris) was used to prepare chromatin. .. The IgG and NF-kB p65 antibodies (2A12A7, Thermo Fisher) were used for immunoprecipitation by the Quick Chip Kit (Imgenex SYBR Green Master Mix, (Appliedbiosystems) was used for qPCR to quantify precipitated DNA).

    Immunoprecipitation:

    Article Title: IRF5 regulates unique subset of genes in dendritic cells during West Nile virus infection
    Article Snippet: Chromatin was isolated using a truChIP chromatin shearing kit (Covaris) and sheared using M220 focused-ultrasonicator (Covaris). .. Immunoprecipitation was performed with validated IRF5 antibodies (Bethyl A303–385 and A303–386).

    Article Title: Single-Cell RNA-Seq Reveals TOX as a Key Regulator of CD8+ T cell Persistence in Chronic Infection
    Article Snippet: DNA-protein crosslinking, nuclei isolation, and chromatin sonication were performed using truChIP Chromatin Shearing Kit (Covaris) according to manufacturer’s instructions. .. After immunoprecipitation by anti-H3K27Ac (ab4729, AbCam), ChIPmentation was performed according to the published protocol .

    Article Title: Protein arginine methyltransferase 5 promotes bladder cancer growth through inhibiting NF-kB dependent apoptosis
    Article Snippet: The truChIP Chromatin Shearing Kit (Covaris) was used to prepare chromatin. .. The IgG and NF-kB p65 antibodies (2A12A7, Thermo Fisher) were used for immunoprecipitation by the Quick Chip Kit (Imgenex SYBR Green Master Mix, (Appliedbiosystems) was used for qPCR to quantify precipitated DNA).

    Article Title: Epigenetic mechanisms underlying NMDA receptor hypofunction in the prefrontal cortex of juvenile animals in the MAM model for schizophrenia
    Article Snippet: Paragraph title: Chromatin immunoprecipitation-qPCR assay ... mPFC tissue was collected from saline and MAM animals at P21 and pooled for the chromatin immunoprecipitation (ChIP) assays performed using the truChIP™ Chromatin Shearing Kit (Covaris, PN 520083).

    Article Title: Protein arginine methyltransferase 5 promotes bladder cancer growth through inhibiting NF-kB dependent apoptosis
    Article Snippet: Chromatin immunoprecipitation (ChIP) The truChIP Chromatin Shearing Kit (Covaris) was used to prepare chromatin. .. The IgG and NF-kB p65 antibodies (2A12A7, Thermo Fisher) were used for immunoprecipitation by the Quick Chip Kit (Imgenex SYBR Green Master Mix, (Appliedbiosystems) was used for qPCR to quantify precipitated DNA).

    Article Title: Enhancer histone-QTLs are enriched on autoimmune risk haplotypes and influence gene expression within chromatin networks
    Article Snippet: We crosslinked 10 million Epstein-Barr Virus (EBV)-transformed B cells with 1% formaldehyde for 5 min followed by careful cell lysis using the truChIP Chromatin Shearing Kit (Covaris). .. Crosslinked protein/DNA was immunoprecipitated using H3K27ac (rabbit polyclonal, Abcam #ab4729, 20ug/ml) or H3K4me1 (rabbit polyclonal, Abcam #ab8895, 20 µg/ml) antibodies on Protein A + G immunomagnetic beads.

    Incubation:

    Article Title: Intestinal, but not hepatic, ChREBP is required for fructose tolerance
    Article Snippet: Chromatin was isolated using a truChIP Chromatin Shearing Tissue Kit (Covaris) according to the manufacturer’s protocol, with modifications. .. Cross-linked tissue was dounce homogenized in Lysis Buffer with protease inhibitors, incubated for 20 minutes at 4°C with periodic vortexing, and crude nuclei were collected by centrifugation at 1,700 g and washed in Wash Buffer twice.

    Article Title: Biglycan- and Sphingosine Kinase-1 Signaling Crosstalk Regulates the Synthesis of Macrophage Chemoattractants
    Article Snippet: Chromatin Immunoprecipitation Preparation of cell extracts, crosslinking and isolation of nuclei was performed with the truCHIP™ Chromatin Shearing Kit (Covaris, Woburn, MA, USA) according to the manufacturers protocol. .. The samples were incubated over night at 4 °C with the antibodies against NF-κB (3 µg Santa Cruz #sc-109, Hedelberg, Germany) or IgG (3 µg Diagenode # c15410206).

    Article Title: TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases
    Article Snippet: Collected cells were fixed with freshly prepared 1% methanol-free formaldehyde at room temperature for 5 min. Chromatin shearing was performed using truChIP Chromatin Shearing Kit with Formaldehyde (Covaris) on E220 (Covaris) according to the manufacturer’s manual. .. Briefly, fragmented chromatin was incubated with protein A dynabeads (Invitrogen) pre-coupled with rabbit anti-TCF1 sera at 4°C overnight.

    Article Title: Distinct Requirements of CHD4 during B Cell Development and Antibody Response
    Article Snippet: Cross-linked cells were washed with PBS-based Cell Wash Buffer (PBS, 0.1% FBS, 2mM EDTA) and lysed according to the protocol of truChIP Chromatin Shearing Kit with Formaldehyde (Covaris, 520154). .. Pre-cleared lysate was divided into three parts of 500 μL each and incubated with 1 μg of anti-H3 antibody (Abcam), 2 μg of anti-AID antibody ( ) or control IgG (Jackson Immunoresearch Labs) at 4∘ C overnight.

    Polymerase Chain Reaction:

    Article Title: The Dynamic Epigenetic Landscape of the Retina During Development, Reprogramming, and Tumorigenesis
    Article Snippet: The nuclei were isolated and prepared for shearing using TruChIP chromatin shearing kit (Covaris #520127) according to manufacturer’s protocol. .. After de-crosslinking, DNA was extracted using MinElute PCR-purification kit (QIAGEN #28006), quantified using the Quant-iT PicoGreen ds DNA assay (Life Technologies #Q33120) and subjected to qPCR analysis and library construction for sequencing.

    Article Title: Epigenetic mechanisms underlying NMDA receptor hypofunction in the prefrontal cortex of juvenile animals in the MAM model for schizophrenia
    Article Snippet: mPFC tissue was collected from saline and MAM animals at P21 and pooled for the chromatin immunoprecipitation (ChIP) assays performed using the truChIP™ Chromatin Shearing Kit (Covaris, PN 520083). .. The eluted material was purified using a QIAquick PCR kit (Qiagen; 28104) and was directly used for qPCR.

    ChIP-sequencing:

    Article Title: The Dynamic Epigenetic Landscape of the Retina During Development, Reprogramming, and Tumorigenesis
    Article Snippet: Paragraph title: ChIP-Seq ... The nuclei were isolated and prepared for shearing using TruChIP chromatin shearing kit (Covaris #520127) according to manufacturer’s protocol.

    Article Title: Single-Cell RNA-Seq Reveals TOX as a Key Regulator of CD8+ T cell Persistence in Chronic Infection
    Article Snippet: Paragraph title: Chromatin immunoprecipitation sequencing (ChIP-seq) ... DNA-protein crosslinking, nuclei isolation, and chromatin sonication were performed using truChIP Chromatin Shearing Kit (Covaris) according to manufacturer’s instructions.

    Isolation:

    Article Title: Intestinal, but not hepatic, ChREBP is required for fructose tolerance
    Article Snippet: .. Chromatin was isolated using a truChIP Chromatin Shearing Tissue Kit (Covaris) according to the manufacturer’s protocol, with modifications. ..

    Article Title: Biglycan- and Sphingosine Kinase-1 Signaling Crosstalk Regulates the Synthesis of Macrophage Chemoattractants
    Article Snippet: .. Chromatin Immunoprecipitation Preparation of cell extracts, crosslinking and isolation of nuclei was performed with the truCHIP™ Chromatin Shearing Kit (Covaris, Woburn, MA, USA) according to the manufacturers protocol. .. After sonification of the lysates with the Bioruptur Plus (10 cycles, 30 s on, 90 s off, 4 °C; Diagenode, Seraing, Belgium), cell debris was removed by centrifugation and the lysates were diluted 1:3 in dilution buffer (20 mmol/L Tris/HCl pH 7.4, 100 mmol/L NaCl, 2 mmol/L ethylenediaminetetraacetic acid (EDTA), 0.5% Triton X-100 and protease inhibitors).

    Article Title: The Dynamic Epigenetic Landscape of the Retina During Development, Reprogramming, and Tumorigenesis
    Article Snippet: .. The nuclei were isolated and prepared for shearing using TruChIP chromatin shearing kit (Covaris #520127) according to manufacturer’s protocol. .. After shearing, ChIP was performed using the iDeal ChIP-seq kit (Diagenode # C01010051).

    Article Title: IRF5 regulates unique subset of genes in dendritic cells during West Nile virus infection
    Article Snippet: .. Chromatin was isolated using a truChIP chromatin shearing kit (Covaris) and sheared using M220 focused-ultrasonicator (Covaris). .. Sheared chromatin was run on an agarose gel and band corresponding to size 200–700bp was extracted.

    Article Title: Single-Cell RNA-Seq Reveals TOX as a Key Regulator of CD8+ T cell Persistence in Chronic Infection
    Article Snippet: .. DNA-protein crosslinking, nuclei isolation, and chromatin sonication were performed using truChIP Chromatin Shearing Kit (Covaris) according to manufacturer’s instructions. .. After immunoprecipitation by anti-H3K27Ac (ab4729, AbCam), ChIPmentation was performed according to the published protocol .

    Article Title: TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases
    Article Snippet: Chromatin immunoprecipitation Tregs were isolated by magnetic separation from pooled spleens and LNs of Foxp3Thy1.1 mice and CD4+ T cells from CD4Cre ;Tcf7fl/fl mice. .. Collected cells were fixed with freshly prepared 1% methanol-free formaldehyde at room temperature for 5 min. Chromatin shearing was performed using truChIP Chromatin Shearing Kit with Formaldehyde (Covaris) on E220 (Covaris) according to the manufacturer’s manual.

    Article Title: Distinct Requirements of CHD4 during B Cell Development and Antibody Response
    Article Snippet: Chromatin immunoprecipitation Splenic B cells from 8–10 week old mice were isolated and ChIP was performed according to protocol described in the ChIP Assay Kit (Millipore, 17–295). .. Cross-linked cells were washed with PBS-based Cell Wash Buffer (PBS, 0.1% FBS, 2mM EDTA) and lysed according to the protocol of truChIP Chromatin Shearing Kit with Formaldehyde (Covaris, 520154).

    Article Title: Enhancer histone-QTLs are enriched on autoimmune risk haplotypes and influence gene expression within chromatin networks
    Article Snippet: We crosslinked 10 million Epstein-Barr Virus (EBV)-transformed B cells with 1% formaldehyde for 5 min followed by careful cell lysis using the truChIP Chromatin Shearing Kit (Covaris). .. Nuclei were isolated and fragmented using a Covaris E220e sonicator with the following operating conditions: Peak Incident Power: 140 Watts; duty cycle: 5%; cycles per burst: 200; treatment time: 15 min; setpoint temperature: 6 °C).

    Sequencing:

    Article Title: The Dynamic Epigenetic Landscape of the Retina During Development, Reprogramming, and Tumorigenesis
    Article Snippet: The nuclei were isolated and prepared for shearing using TruChIP chromatin shearing kit (Covaris #520127) according to manufacturer’s protocol. .. After de-crosslinking, DNA was extracted using MinElute PCR-purification kit (QIAGEN #28006), quantified using the Quant-iT PicoGreen ds DNA assay (Life Technologies #Q33120) and subjected to qPCR analysis and library construction for sequencing.

    Article Title: Enhancer histone-QTLs are enriched on autoimmune risk haplotypes and influence gene expression within chromatin networks
    Article Snippet: Paragraph title: ChIP sequencing and analysis ... We crosslinked 10 million Epstein-Barr Virus (EBV)-transformed B cells with 1% formaldehyde for 5 min followed by careful cell lysis using the truChIP Chromatin Shearing Kit (Covaris).

    FACS:

    Article Title: Single-Cell RNA-Seq Reveals TOX as a Key Regulator of CD8+ T cell Persistence in Chronic Infection
    Article Snippet: Chromatin immunoprecipitation sequencing (ChIP-seq) 1 × 106 FACS-sorted stem-like (Blimp-1-YFPlo Tim3lo ), terminally exhausted (Blimp-1-YFPhi Tim3hi ), memory precursor (KLRG1lo ), and short-lived effector (KLRG1hi ) P14 cells were used for ChIP analyses. .. DNA-protein crosslinking, nuclei isolation, and chromatin sonication were performed using truChIP Chromatin Shearing Kit (Covaris) according to manufacturer’s instructions.

    Lysis:

    Article Title: Intestinal, but not hepatic, ChREBP is required for fructose tolerance
    Article Snippet: Chromatin was isolated using a truChIP Chromatin Shearing Tissue Kit (Covaris) according to the manufacturer’s protocol, with modifications. .. Cross-linked tissue was dounce homogenized in Lysis Buffer with protease inhibitors, incubated for 20 minutes at 4°C with periodic vortexing, and crude nuclei were collected by centrifugation at 1,700 g and washed in Wash Buffer twice.

    Article Title: Enhancer histone-QTLs are enriched on autoimmune risk haplotypes and influence gene expression within chromatin networks
    Article Snippet: .. We crosslinked 10 million Epstein-Barr Virus (EBV)-transformed B cells with 1% formaldehyde for 5 min followed by careful cell lysis using the truChIP Chromatin Shearing Kit (Covaris). .. Nuclei were isolated and fragmented using a Covaris E220e sonicator with the following operating conditions: Peak Incident Power: 140 Watts; duty cycle: 5%; cycles per burst: 200; treatment time: 15 min; setpoint temperature: 6 °C).

    Article Title: Zc3h10 Acts as a Transcription Factor and Is Phosphorylated to Activate the Thermogenic Program
    Article Snippet: Crosslinked was performed using 1% methanol-free formaldehyde in crosslinking buffer from the truChIP chromatin shearing kit (Covaris) for 5 min. .. The manufacturer recommended protocol was followed with the exception of adding 2 dounce steps following the neutralization of the crosslinking with the loose pestle and after addition of the lysis buffer with the tight pestle.

    Mouse Assay:

    Article Title: TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases
    Article Snippet: Chromatin immunoprecipitation Tregs were isolated by magnetic separation from pooled spleens and LNs of Foxp3Thy1.1 mice and CD4+ T cells from CD4Cre ;Tcf7fl/fl mice. .. Collected cells were fixed with freshly prepared 1% methanol-free formaldehyde at room temperature for 5 min. Chromatin shearing was performed using truChIP Chromatin Shearing Kit with Formaldehyde (Covaris) on E220 (Covaris) according to the manufacturer’s manual.

    Article Title: Distinct Requirements of CHD4 during B Cell Development and Antibody Response
    Article Snippet: Chromatin immunoprecipitation Splenic B cells from 8–10 week old mice were isolated and ChIP was performed according to protocol described in the ChIP Assay Kit (Millipore, 17–295). .. Cross-linked cells were washed with PBS-based Cell Wash Buffer (PBS, 0.1% FBS, 2mM EDTA) and lysed according to the protocol of truChIP Chromatin Shearing Kit with Formaldehyde (Covaris, 520154).

    Chromatin Immunoprecipitation:

    Article Title: Nuclear Innate Immune DNA Sensor IFI16 Is Degraded during Lytic Reactivation of Kaposi's Sarcoma-Associated Herpesvirus (KSHV): Role of IFI16 in Maintenance of KSHV Latency
    Article Snippet: .. B-cell chromatin shearing for ChIP was performed by using the truChIP Chromatin Shearing kit (Covaris, Woburn, MA) according to the manufacturer's instructions. .. Chromatin was sheared in shearing buffer (truChIP Chromatin Shearing kit) by using a Covaris E220 focused ultrasonicator.

    Article Title: Intestinal, but not hepatic, ChREBP is required for fructose tolerance
    Article Snippet: Paragraph title: ChIP-PCR. ... Chromatin was isolated using a truChIP Chromatin Shearing Tissue Kit (Covaris) according to the manufacturer’s protocol, with modifications.

    Article Title: Biglycan- and Sphingosine Kinase-1 Signaling Crosstalk Regulates the Synthesis of Macrophage Chemoattractants
    Article Snippet: .. Chromatin Immunoprecipitation Preparation of cell extracts, crosslinking and isolation of nuclei was performed with the truCHIP™ Chromatin Shearing Kit (Covaris, Woburn, MA, USA) according to the manufacturers protocol. .. After sonification of the lysates with the Bioruptur Plus (10 cycles, 30 s on, 90 s off, 4 °C; Diagenode, Seraing, Belgium), cell debris was removed by centrifugation and the lysates were diluted 1:3 in dilution buffer (20 mmol/L Tris/HCl pH 7.4, 100 mmol/L NaCl, 2 mmol/L ethylenediaminetetraacetic acid (EDTA), 0.5% Triton X-100 and protease inhibitors).

    Article Title: The Dynamic Epigenetic Landscape of the Retina During Development, Reprogramming, and Tumorigenesis
    Article Snippet: Freshly isolated retinae or cells were cross-linked for 10 min in 1% ChIP-seq–grade formaldehyde (Thermo scientific # 28906) in 1X PBS at room temperature. .. The nuclei were isolated and prepared for shearing using TruChIP chromatin shearing kit (Covaris #520127) according to manufacturer’s protocol.

    Article Title: JunB defines functional and structural integrity of the epidermo-pilosebaceous unit in the skin
    Article Snippet: Paragraph title: ChIP assay ... Briefly, 10 × 106 epidermal progenitor cells were subjected to fixation, nuclei preparation and chromatin fragmentation using truChIP Chromatin Shearing Kit (Covaris).

    Article Title: IRF5 regulates unique subset of genes in dendritic cells during West Nile virus infection
    Article Snippet: Paragraph title: ChIP-exo ... Chromatin was isolated using a truChIP chromatin shearing kit (Covaris) and sheared using M220 focused-ultrasonicator (Covaris).

    Article Title: Single-Cell RNA-Seq Reveals TOX as a Key Regulator of CD8+ T cell Persistence in Chronic Infection
    Article Snippet: Chromatin immunoprecipitation sequencing (ChIP-seq) 1 × 106 FACS-sorted stem-like (Blimp-1-YFPlo Tim3lo ), terminally exhausted (Blimp-1-YFPhi Tim3hi ), memory precursor (KLRG1lo ), and short-lived effector (KLRG1hi ) P14 cells were used for ChIP analyses. .. DNA-protein crosslinking, nuclei isolation, and chromatin sonication were performed using truChIP Chromatin Shearing Kit (Covaris) according to manufacturer’s instructions.

    Article Title: Protein arginine methyltransferase 5 promotes bladder cancer growth through inhibiting NF-kB dependent apoptosis
    Article Snippet: Paragraph title: Chromatin immunoprecipitation (ChIP) ... The truChIP Chromatin Shearing Kit (Covaris) was used to prepare chromatin.

    Article Title: T Cell Receptor-induced Nuclear Factor κB (NF-κB) Signaling and Transcriptional Activation Are Regulated by STIM1- and Orai1-mediated Calcium Entry *
    Article Snippet: Paragraph title: Chromatin Immunoprecipitation ... Chromatin was prepared using a Covaris truChIP chromatin shearing kit (Covaris Inc., Woburn, MA).

    Article Title: TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases
    Article Snippet: Paragraph title: Chromatin immunoprecipitation ... Collected cells were fixed with freshly prepared 1% methanol-free formaldehyde at room temperature for 5 min. Chromatin shearing was performed using truChIP Chromatin Shearing Kit with Formaldehyde (Covaris) on E220 (Covaris) according to the manufacturer’s manual.

    Article Title: Distinct Requirements of CHD4 during B Cell Development and Antibody Response
    Article Snippet: Paragraph title: Chromatin immunoprecipitation ... Cross-linked cells were washed with PBS-based Cell Wash Buffer (PBS, 0.1% FBS, 2mM EDTA) and lysed according to the protocol of truChIP Chromatin Shearing Kit with Formaldehyde (Covaris, 520154).

    Article Title: Epigenetic mechanisms underlying NMDA receptor hypofunction in the prefrontal cortex of juvenile animals in the MAM model for schizophrenia
    Article Snippet: .. mPFC tissue was collected from saline and MAM animals at P21 and pooled for the chromatin immunoprecipitation (ChIP) assays performed using the truChIP™ Chromatin Shearing Kit (Covaris, PN 520083). .. Three separate reactions with 50 mg of sheared chromatin were carried out using: (i) 2 μg of REST antibody (RRID:AB_310728; Millipore; 07-579), (ii) 1 μg H3K27me3 antibody (Abcam; ab6002), and (iii) a mock reaction containing all reagents with IgG as a control.

    Article Title: Protein arginine methyltransferase 5 promotes bladder cancer growth through inhibiting NF-kB dependent apoptosis
    Article Snippet: .. Chromatin immunoprecipitation (ChIP) The truChIP Chromatin Shearing Kit (Covaris) was used to prepare chromatin. .. Chromatin was sheared into 200- to 700-bp fragments using a Covaris S2 instrument (duty cycle, 2 %; intensity, 3; 200 cycles per burst; 4 min).

    Article Title: Enhancer histone-QTLs are enriched on autoimmune risk haplotypes and influence gene expression within chromatin networks
    Article Snippet: Paragraph title: ChIP sequencing and analysis ... We crosslinked 10 million Epstein-Barr Virus (EBV)-transformed B cells with 1% formaldehyde for 5 min followed by careful cell lysis using the truChIP Chromatin Shearing Kit (Covaris).

    Article Title: Zc3h10 Acts as a Transcription Factor and Is Phosphorylated to Activate the Thermogenic Program
    Article Snippet: Paragraph title: ChIP and ChIPseq ... Crosslinked was performed using 1% methanol-free formaldehyde in crosslinking buffer from the truChIP chromatin shearing kit (Covaris) for 5 min.

    Plasmid Preparation:

    Article Title: T Cell Receptor-induced Nuclear Factor κB (NF-κB) Signaling and Transcriptional Activation Are Regulated by STIM1- and Orai1-mediated Calcium Entry *
    Article Snippet: Jurkat T cells (10 × 106 ) transfected with either EGFP-shPKCα or control EGFP-pCMS2 vector (48 h) were stimulated with PMA (200 n m ) and ionomycin (1 μ m ) for 30 min at 37 °C. .. Chromatin was prepared using a Covaris truChIP chromatin shearing kit (Covaris Inc., Woburn, MA).

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    Covaris truchip chromatin shearing kit
    Truchip Chromatin Shearing Kit, supplied by Covaris, used in various techniques. Bioz Stars score: 95/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truchip chromatin shearing kit/product/Covaris
    Average 95 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    truchip chromatin shearing kit - by Bioz Stars, 2020-02
    95/100 stars
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    Quantity 10 of Covaris truChIP Chromatin Shearing Kit with Formaldehyde 520154
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