trpv6  (Alomone Labs)


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    Alomone Labs trpv6
    E2, BPA, and OP change the expressions of <t>TRPV6</t> , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p
    Trpv6, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse"

    Article Title: Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse

    Journal: International Journal of Environmental Research and Public Health

    doi: 10.3390/ijerph15081614

    E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p
    Figure Legend Snippet: E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p

    Techniques Used: Mouse Assay, Injection, Real-time Polymerase Chain Reaction, Expressing

    2) Product Images from "Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse"

    Article Title: Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse

    Journal: International Journal of Environmental Research and Public Health

    doi: 10.3390/ijerph15081614

    E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p
    Figure Legend Snippet: E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p

    Techniques Used: Mouse Assay, Injection, Real-time Polymerase Chain Reaction, Expressing

    3) Product Images from "Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse"

    Article Title: Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse

    Journal: International Journal of Environmental Research and Public Health

    doi: 10.3390/ijerph15081614

    E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p
    Figure Legend Snippet: E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p

    Techniques Used: Mouse Assay, Injection, Real-time Polymerase Chain Reaction, Expressing

    4) Product Images from "Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse"

    Article Title: Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse

    Journal: International Journal of Environmental Research and Public Health

    doi: 10.3390/ijerph15081614

    E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p
    Figure Legend Snippet: E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p

    Techniques Used: Mouse Assay, Injection, Real-time Polymerase Chain Reaction, Expressing

    5) Product Images from "Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse"

    Article Title: Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse

    Journal: International Journal of Environmental Research and Public Health

    doi: 10.3390/ijerph15081614

    E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p
    Figure Legend Snippet: E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p

    Techniques Used: Mouse Assay, Injection, Real-time Polymerase Chain Reaction, Expressing

    6) Product Images from "Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse"

    Article Title: Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse

    Journal: International Journal of Environmental Research and Public Health

    doi: 10.3390/ijerph15081614

    E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p
    Figure Legend Snippet: E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p

    Techniques Used: Mouse Assay, Injection, Real-time Polymerase Chain Reaction, Expressing

    7) Product Images from "Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse"

    Article Title: Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse

    Journal: International Journal of Environmental Research and Public Health

    doi: 10.3390/ijerph15081614

    E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p
    Figure Legend Snippet: E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p

    Techniques Used: Mouse Assay, Injection, Real-time Polymerase Chain Reaction, Expressing

    8) Product Images from "Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse"

    Article Title: Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse

    Journal: International Journal of Environmental Research and Public Health

    doi: 10.3390/ijerph15081614

    E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p
    Figure Legend Snippet: E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p

    Techniques Used: Mouse Assay, Injection, Real-time Polymerase Chain Reaction, Expressing

    9) Product Images from "Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse"

    Article Title: Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse

    Journal: International Journal of Environmental Research and Public Health

    doi: 10.3390/ijerph15081614

    E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p
    Figure Legend Snippet: E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p

    Techniques Used: Mouse Assay, Injection, Real-time Polymerase Chain Reaction, Expressing

    10) Product Images from "Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse"

    Article Title: Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse

    Journal: International Journal of Environmental Research and Public Health

    doi: 10.3390/ijerph15081614

    E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p
    Figure Legend Snippet: E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p

    Techniques Used: Mouse Assay, Injection, Real-time Polymerase Chain Reaction, Expressing

    11) Product Images from "Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse"

    Article Title: Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse

    Journal: International Journal of Environmental Research and Public Health

    doi: 10.3390/ijerph15081614

    E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p
    Figure Legend Snippet: E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p

    Techniques Used: Mouse Assay, Injection, Real-time Polymerase Chain Reaction, Expressing

    12) Product Images from "Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse"

    Article Title: Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse

    Journal: International Journal of Environmental Research and Public Health

    doi: 10.3390/ijerph15081614

    E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p
    Figure Legend Snippet: E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p

    Techniques Used: Mouse Assay, Injection, Real-time Polymerase Chain Reaction, Expressing

    13) Product Images from "Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse"

    Article Title: Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse

    Journal: International Journal of Environmental Research and Public Health

    doi: 10.3390/ijerph15081614

    E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p
    Figure Legend Snippet: E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p

    Techniques Used: Mouse Assay, Injection, Real-time Polymerase Chain Reaction, Expressing

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    Alomone Labs trpv6
    E2, BPA, and OP change the expressions of <t>TRPV6</t> , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p
    Trpv6, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs rabbit anti trpv5
    Channel characteristics of wild-type and mutant <t>TRPV5.</t> ( A ) Whole-cell currents in TRPV5-WT (V5-WT) and TRPV5-682P (V5-S682P) injected Xenopus oocytes recorded in response to 300 ms test pulses to various potentials (from −100 to +60 mV in 10 mV increments). Holding potential, 0 mV (N = 5). ( B ) Mean current-voltage relationships for TRPV5-WT and TRPV5-682P channels (N = 5). These current-voltage relationships are similar to those reported for TRPV5 channels [62] . ( C ) Mean whole-cell tail currents measured in TRPV5-WT and TRPV5-682P injected Xenopus oocytes during test potentials applied in 10 mV increments from −70 to +40 mV after a pre-pulse to −100 mV in TRPV5-WT and TRPV5-682P channels (N = 5). ( D ) Time-dependent inhibition of TRPV-WT and TRPV5-682P whole-cell currents. Oocytes were stimulated every 1 s. The peak current amplitude was normalised to that recorded during the first pulse (N = 4). ( E ) Representative trace of Fura-2 ratio in HEK293 cells transiently transfected with an empty EGFP vector (mock), or EGFP-tagged TRPV5-WT or TRPV5-S682P. Cells expressing EGFP were selected and monitored for changes in intracellular Ca 2+ levels when extracellular Ca 2+ concentrations were varied from 1.4 mM Ca 2+ to 0 mM Ca 2+ (2 mM EDTA) and 1.4 mM Ca 2+ which was facilitated by superfusion. ( F ) Fura-2 levels under resting conditions (t0), minimal Fura-2 ratio after EDTA treatment (tmin) and peak level (tmax) upon administration of 1.4 mM Ca 2+ after EDTA treatment. Average data of cells transfected with the empty vector (n = 7), TRPV5-wt (n = 24) and TRPV5-S682P (N = 24) from at least three independent experiments. * p
    Rabbit Anti Trpv5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p

    Journal: International Journal of Environmental Research and Public Health

    Article Title: Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse

    doi: 10.3390/ijerph15081614

    Figure Lengend Snippet: E2, BPA, and OP change the expressions of TRPV6 , TRPV5 , PMCA1 , and NCX1 in maternal uterus and implantation sites. Mice were sacrificed at GD 4.5 (24 h after final injection) to collect uterus tissues and at GD 5.5 (after 48 h after final injection) to collect implantation sites. The mRNA expressions of calcium transporter channel genes in uterus and implantation sites were assessed. The mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 genes were measured by using real-time PCR and were normalized to that of 18S ribosomal RNA (RN18S). In uterus, ( a ) the expressions of TRPV5 mRNA were significantly high in the E2, E2 + ICI, BPA + ICI, OP, and OP + ICI groups; ( b ) TRPV6 mRNA level changes were similar to those for TRPV5 expression. mRNA expression of TRPV5 and TRPV6 in uteri were higher in BPA + ICI group than in the BPA group; ( c , d ) mRNA level of PMCA1 and NCX1 were significantly decreased by E2 and E2 + ICI. In implantation sites; ( e – h ) the mRNA levels of TRPV6 , TRPV5 , PMCA1 , and NCX1 , respectively, were markedly low in all groups. Statistical significance was determined by two-way ANOVA. * p

    Article Snippet: The mRNA expression levels of TRPV5 , TRPV6 , PMCA1 , and NCX1 were markedly low in the BPA- and OP-treated implantation sites ( e–h).

    Techniques: Mouse Assay, Injection, Real-time Polymerase Chain Reaction, Expressing

    Channel characteristics of wild-type and mutant TRPV5. ( A ) Whole-cell currents in TRPV5-WT (V5-WT) and TRPV5-682P (V5-S682P) injected Xenopus oocytes recorded in response to 300 ms test pulses to various potentials (from −100 to +60 mV in 10 mV increments). Holding potential, 0 mV (N = 5). ( B ) Mean current-voltage relationships for TRPV5-WT and TRPV5-682P channels (N = 5). These current-voltage relationships are similar to those reported for TRPV5 channels [62] . ( C ) Mean whole-cell tail currents measured in TRPV5-WT and TRPV5-682P injected Xenopus oocytes during test potentials applied in 10 mV increments from −70 to +40 mV after a pre-pulse to −100 mV in TRPV5-WT and TRPV5-682P channels (N = 5). ( D ) Time-dependent inhibition of TRPV-WT and TRPV5-682P whole-cell currents. Oocytes were stimulated every 1 s. The peak current amplitude was normalised to that recorded during the first pulse (N = 4). ( E ) Representative trace of Fura-2 ratio in HEK293 cells transiently transfected with an empty EGFP vector (mock), or EGFP-tagged TRPV5-WT or TRPV5-S682P. Cells expressing EGFP were selected and monitored for changes in intracellular Ca 2+ levels when extracellular Ca 2+ concentrations were varied from 1.4 mM Ca 2+ to 0 mM Ca 2+ (2 mM EDTA) and 1.4 mM Ca 2+ which was facilitated by superfusion. ( F ) Fura-2 levels under resting conditions (t0), minimal Fura-2 ratio after EDTA treatment (tmin) and peak level (tmax) upon administration of 1.4 mM Ca 2+ after EDTA treatment. Average data of cells transfected with the empty vector (n = 7), TRPV5-wt (n = 24) and TRPV5-S682P (N = 24) from at least three independent experiments. * p

    Journal: PLoS ONE

    Article Title: Autosomal Dominant Hypercalciuria in a Mouse Model Due to a Mutation of the Epithelial Calcium Channel, TRPV5

    doi: 10.1371/journal.pone.0055412

    Figure Lengend Snippet: Channel characteristics of wild-type and mutant TRPV5. ( A ) Whole-cell currents in TRPV5-WT (V5-WT) and TRPV5-682P (V5-S682P) injected Xenopus oocytes recorded in response to 300 ms test pulses to various potentials (from −100 to +60 mV in 10 mV increments). Holding potential, 0 mV (N = 5). ( B ) Mean current-voltage relationships for TRPV5-WT and TRPV5-682P channels (N = 5). These current-voltage relationships are similar to those reported for TRPV5 channels [62] . ( C ) Mean whole-cell tail currents measured in TRPV5-WT and TRPV5-682P injected Xenopus oocytes during test potentials applied in 10 mV increments from −70 to +40 mV after a pre-pulse to −100 mV in TRPV5-WT and TRPV5-682P channels (N = 5). ( D ) Time-dependent inhibition of TRPV-WT and TRPV5-682P whole-cell currents. Oocytes were stimulated every 1 s. The peak current amplitude was normalised to that recorded during the first pulse (N = 4). ( E ) Representative trace of Fura-2 ratio in HEK293 cells transiently transfected with an empty EGFP vector (mock), or EGFP-tagged TRPV5-WT or TRPV5-S682P. Cells expressing EGFP were selected and monitored for changes in intracellular Ca 2+ levels when extracellular Ca 2+ concentrations were varied from 1.4 mM Ca 2+ to 0 mM Ca 2+ (2 mM EDTA) and 1.4 mM Ca 2+ which was facilitated by superfusion. ( F ) Fura-2 levels under resting conditions (t0), minimal Fura-2 ratio after EDTA treatment (tmin) and peak level (tmax) upon administration of 1.4 mM Ca 2+ after EDTA treatment. Average data of cells transfected with the empty vector (n = 7), TRPV5-wt (n = 24) and TRPV5-S682P (N = 24) from at least three independent experiments. * p

    Article Snippet: For TRPV5 immuno-detection, 8- µm kidney cryosections were processed for immunofluorescence labelling as previously described ._ENREF_41 Kidney cryosections were co-stained with rabbit anti-TRPV5 (ACC-035, Alomone Labs, Jerusalem, Israel) and goat anti-AQP2 (sc-9882, Santa Cruz, Insight Biotechnology, Wembley, UK) polyclonal antibodies, or with goat anti-TRPV5 (sc-23379, Santa Cruz) and rabbit anti-NCC polyclonal antibodies, followed by the appropriate Alexa Fluor 488- or 594-conjugated secondary antibodies (Molecular Probes).

    Techniques: Mutagenesis, Injection, Mass Spectrometry, Inhibition, Transfection, Plasmid Preparation, Expressing

    Assessment of Renal Expression of Calcium Regulatory Genes and Proteins. Renal expression of ( A ) Trpv5 , ( B ) Trpv6 and ( C ) Cyp24a1 in wild-type (wt), Trpv5 682P/+ (het) and Trpv5 682P/682P (hom) mice (n = 6/group) were assessed by quantitative real-time PCR. All data were normalised to levels of the housekeeping gene Gapdh and wild-type values are expressed as 1. Histogram data are presented as mean ± SEM. # p

    Journal: PLoS ONE

    Article Title: Autosomal Dominant Hypercalciuria in a Mouse Model Due to a Mutation of the Epithelial Calcium Channel, TRPV5

    doi: 10.1371/journal.pone.0055412

    Figure Lengend Snippet: Assessment of Renal Expression of Calcium Regulatory Genes and Proteins. Renal expression of ( A ) Trpv5 , ( B ) Trpv6 and ( C ) Cyp24a1 in wild-type (wt), Trpv5 682P/+ (het) and Trpv5 682P/682P (hom) mice (n = 6/group) were assessed by quantitative real-time PCR. All data were normalised to levels of the housekeeping gene Gapdh and wild-type values are expressed as 1. Histogram data are presented as mean ± SEM. # p

    Article Snippet: For TRPV5 immuno-detection, 8- µm kidney cryosections were processed for immunofluorescence labelling as previously described ._ENREF_41 Kidney cryosections were co-stained with rabbit anti-TRPV5 (ACC-035, Alomone Labs, Jerusalem, Israel) and goat anti-AQP2 (sc-9882, Santa Cruz, Insight Biotechnology, Wembley, UK) polyclonal antibodies, or with goat anti-TRPV5 (sc-23379, Santa Cruz) and rabbit anti-NCC polyclonal antibodies, followed by the appropriate Alexa Fluor 488- or 594-conjugated secondary antibodies (Molecular Probes).

    Techniques: Expressing, Mouse Assay, Real-time Polymerase Chain Reaction

    Histological and immunohistochemical assessment of kidneys from HCALC1 mice. Representative images in Trpv5 +/+ (wt), Trpv5 682P/+ (het) and Trpv5 682P/682P (hom) mice of: ( A ) Masson's trichrome staining of renal cortex showing areas of interstitial fibrosis in Trpv5 682P/+ and Trpv5 682P/682P mice (light blue), ( B ) anti-CD3-labelling (green) showing a large number of T-lymphocytes present in the interstitial regions of the Trpv5 682P/+ and Trpv5 682P/682P mouse kidneys, ( C ) TUNEL-labelling (green) of the renal cortex showing the presence of tubular cell apoptosis in the Trpv5 682P/+ and Trpv5 682P/682P mouse kidneys. Scale bar = 50 µm. ( D ) Immunohistochemical images of kidney sections from wild-type (wt), Trpv5 682P/+ (het) and Trpv5 682P/682P (hom) mice, co-stained for TRPV5 (green) and NCC (red). * denotes co-localisation. Scale bar = 50 µm. ( E ) Kidney sections from wild-type (wt), Trpv5 682P/+ (het) and Trpv5 682P/682P (hom) mice, co-stained for TRPV5 (green) and AQP2 (red). * denotes co-localisation. Scale bar = 50 µm.

    Journal: PLoS ONE

    Article Title: Autosomal Dominant Hypercalciuria in a Mouse Model Due to a Mutation of the Epithelial Calcium Channel, TRPV5

    doi: 10.1371/journal.pone.0055412

    Figure Lengend Snippet: Histological and immunohistochemical assessment of kidneys from HCALC1 mice. Representative images in Trpv5 +/+ (wt), Trpv5 682P/+ (het) and Trpv5 682P/682P (hom) mice of: ( A ) Masson's trichrome staining of renal cortex showing areas of interstitial fibrosis in Trpv5 682P/+ and Trpv5 682P/682P mice (light blue), ( B ) anti-CD3-labelling (green) showing a large number of T-lymphocytes present in the interstitial regions of the Trpv5 682P/+ and Trpv5 682P/682P mouse kidneys, ( C ) TUNEL-labelling (green) of the renal cortex showing the presence of tubular cell apoptosis in the Trpv5 682P/+ and Trpv5 682P/682P mouse kidneys. Scale bar = 50 µm. ( D ) Immunohistochemical images of kidney sections from wild-type (wt), Trpv5 682P/+ (het) and Trpv5 682P/682P (hom) mice, co-stained for TRPV5 (green) and NCC (red). * denotes co-localisation. Scale bar = 50 µm. ( E ) Kidney sections from wild-type (wt), Trpv5 682P/+ (het) and Trpv5 682P/682P (hom) mice, co-stained for TRPV5 (green) and AQP2 (red). * denotes co-localisation. Scale bar = 50 µm.

    Article Snippet: For TRPV5 immuno-detection, 8- µm kidney cryosections were processed for immunofluorescence labelling as previously described ._ENREF_41 Kidney cryosections were co-stained with rabbit anti-TRPV5 (ACC-035, Alomone Labs, Jerusalem, Israel) and goat anti-AQP2 (sc-9882, Santa Cruz, Insight Biotechnology, Wembley, UK) polyclonal antibodies, or with goat anti-TRPV5 (sc-23379, Santa Cruz) and rabbit anti-NCC polyclonal antibodies, followed by the appropriate Alexa Fluor 488- or 594-conjugated secondary antibodies (Molecular Probes).

    Techniques: Immunohistochemistry, Mouse Assay, Staining, TUNEL Assay

    Hypercalciuria in HCALC1 ENU mutant mice and identification of a Trpv5 mutation. ( A ) Urine calcium/creatinine ratios in 23 G2 offspring of the HCALC1 founder male revealed that 10 of the 23 mice were hypercalciuric, consistent with an autosomal dominant inheritance. Bar, mean calcium/creatinine values. ( B ) Haplotype analysis of 89 G2 mice (39 hypercalciuric and 50 normocalciuric) was initially undertaken separately in the hypercalciuric and normocalciuric mice, as the penetrance of HCALC1 was unknown. Haplotype analysis of the hypercalciuric mice localised Hcalc1 to a 17.38 Mb interval on chromosome 6, flanked by rs13478688 and rs30110406 (broken double-headed arrow). Haplotype analysis using combined data for the hypercalciuric and normocalciuric mice identified the smaller interval, 11.94-Mb, flanked by rs13478709 and rs30110406 (solid double-headed arrow). The Hcalc1 locus is inherited with the C57BL/6J haplotype from the F1 founder male. Filled box, C57BL/6J allele; and open box, C3H/HeH allele. Number of mice observed for each haplotype is shown beneath each column. ( C ) DNA sequence analysis of Trpv5 identified a heterozygous T to C transition in codon 682 in hypercalciuric mice predicted to alter a wild-type serine (Ser) to a mutant proline (Pro). This mutation resulted in gain of a Bsa JI restriction enzyme site that was used to confirm the presence of the mutation in the 39 hypercalciuric mice (n = 3 shown) and its absence in the 50 normocalciuric mice (n = 3 shown). wt, wild-type; m, mutant. ( D ) Amino acid sequence alignment revealed evolutionary conservation of the wild-type mouse TRPV5 serine (S) residue at codon 682 (arrowed) in 5 species, as well as in mouse TRPV6 (mTrpv6). Identical residues are shaded black and conservative changes are shaded grey.

    Journal: PLoS ONE

    Article Title: Autosomal Dominant Hypercalciuria in a Mouse Model Due to a Mutation of the Epithelial Calcium Channel, TRPV5

    doi: 10.1371/journal.pone.0055412

    Figure Lengend Snippet: Hypercalciuria in HCALC1 ENU mutant mice and identification of a Trpv5 mutation. ( A ) Urine calcium/creatinine ratios in 23 G2 offspring of the HCALC1 founder male revealed that 10 of the 23 mice were hypercalciuric, consistent with an autosomal dominant inheritance. Bar, mean calcium/creatinine values. ( B ) Haplotype analysis of 89 G2 mice (39 hypercalciuric and 50 normocalciuric) was initially undertaken separately in the hypercalciuric and normocalciuric mice, as the penetrance of HCALC1 was unknown. Haplotype analysis of the hypercalciuric mice localised Hcalc1 to a 17.38 Mb interval on chromosome 6, flanked by rs13478688 and rs30110406 (broken double-headed arrow). Haplotype analysis using combined data for the hypercalciuric and normocalciuric mice identified the smaller interval, 11.94-Mb, flanked by rs13478709 and rs30110406 (solid double-headed arrow). The Hcalc1 locus is inherited with the C57BL/6J haplotype from the F1 founder male. Filled box, C57BL/6J allele; and open box, C3H/HeH allele. Number of mice observed for each haplotype is shown beneath each column. ( C ) DNA sequence analysis of Trpv5 identified a heterozygous T to C transition in codon 682 in hypercalciuric mice predicted to alter a wild-type serine (Ser) to a mutant proline (Pro). This mutation resulted in gain of a Bsa JI restriction enzyme site that was used to confirm the presence of the mutation in the 39 hypercalciuric mice (n = 3 shown) and its absence in the 50 normocalciuric mice (n = 3 shown). wt, wild-type; m, mutant. ( D ) Amino acid sequence alignment revealed evolutionary conservation of the wild-type mouse TRPV5 serine (S) residue at codon 682 (arrowed) in 5 species, as well as in mouse TRPV6 (mTrpv6). Identical residues are shaded black and conservative changes are shaded grey.

    Article Snippet: For TRPV5 immuno-detection, 8- µm kidney cryosections were processed for immunofluorescence labelling as previously described ._ENREF_41 Kidney cryosections were co-stained with rabbit anti-TRPV5 (ACC-035, Alomone Labs, Jerusalem, Israel) and goat anti-AQP2 (sc-9882, Santa Cruz, Insight Biotechnology, Wembley, UK) polyclonal antibodies, or with goat anti-TRPV5 (sc-23379, Santa Cruz) and rabbit anti-NCC polyclonal antibodies, followed by the appropriate Alexa Fluor 488- or 594-conjugated secondary antibodies (Molecular Probes).

    Techniques: Mutagenesis, Mouse Assay, Sequencing