trpa1  (Alomone Labs)


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    Structured Review

    Alomone Labs trpa1
    WIRS induced up-regulated protein expression of <t>TRPA1</t> in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Trpa1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trpa1/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpa1 - by Bioz Stars, 2022-01
    92/100 stars

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    1) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    2) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    3) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    4) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    5) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    6) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    7) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    8) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    9) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    10) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    11) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    12) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    13) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    14) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    15) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    16) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    17) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    18) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    19) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    20) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    21) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    22) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    23) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    24) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    25) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    26) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    27) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    28) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

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    Alomone Labs trpa1
    WIRS induced up-regulated protein expression of <t>TRPA1</t> in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Trpa1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs anti trpa1 primary antibodies
    Axonal transport of TRPV1 and <t>TRPA1.</t> (A) TRPV1 and (C) TRPA1 immunolabeling proximal to the ligation site in saline-treated, neuritis and vinblastine-treated groups. The ligation was immediately distal to the treatment site. (B, D) Mean ratio of TRPV1 immunolabeling (ligated portion / equivalent unligated portion). P = proximal, D = distal, L = ligated portion of the nerve, UL = unligated portion of the nerve. Insert: Methods schematic showing sciatic nerve in the thigh. Note the red box represents the approximate position of the sections. D = distal, L = ligated portion of the nerve, UL = unligated portion of the nerve. Arrow head = Ligation site. * p
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    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Journal: The Turkish Journal of Gastroenterology

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    doi: 10.5152/tjg.2018.17817

    Figure Lengend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Article Snippet: Nozawa K, Kawabata-Shoda E, Doihara H, et al. TRPA1 regulates gastrointestinal motility through serotonin release from enterochromaffin cells.

    Techniques: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Journal: The Turkish Journal of Gastroenterology

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    doi: 10.5152/tjg.2018.17817

    Figure Lengend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Article Snippet: Nozawa K, Kawabata-Shoda E, Doihara H, et al. TRPA1 regulates gastrointestinal motility through serotonin release from enterochromaffin cells.

    Techniques: Expressing, Western Blot, Immunohistochemistry

    Axonal transport of TRPV1 and TRPA1. (A) TRPV1 and (C) TRPA1 immunolabeling proximal to the ligation site in saline-treated, neuritis and vinblastine-treated groups. The ligation was immediately distal to the treatment site. (B, D) Mean ratio of TRPV1 immunolabeling (ligated portion / equivalent unligated portion). P = proximal, D = distal, L = ligated portion of the nerve, UL = unligated portion of the nerve. Insert: Methods schematic showing sciatic nerve in the thigh. Note the red box represents the approximate position of the sections. D = distal, L = ligated portion of the nerve, UL = unligated portion of the nerve. Arrow head = Ligation site. * p

    Journal: Neuroscience

    Article Title: Characterizing the mechanical properties of ectopic axonal receptive fields in inflamed nerves and following axonal transport disruption

    doi: 10.1016/j.neuroscience.2019.11.042

    Figure Lengend Snippet: Axonal transport of TRPV1 and TRPA1. (A) TRPV1 and (C) TRPA1 immunolabeling proximal to the ligation site in saline-treated, neuritis and vinblastine-treated groups. The ligation was immediately distal to the treatment site. (B, D) Mean ratio of TRPV1 immunolabeling (ligated portion / equivalent unligated portion). P = proximal, D = distal, L = ligated portion of the nerve, UL = unligated portion of the nerve. Insert: Methods schematic showing sciatic nerve in the thigh. Note the red box represents the approximate position of the sections. D = distal, L = ligated portion of the nerve, UL = unligated portion of the nerve. Arrow head = Ligation site. * p

    Article Snippet: Sections were blocked with 4% normal goat serum (Vector Labs, Burlingame, USA) in phosphate-buffered saline (PBS) (Sigma, UK) for 1 hour at room temperature and incubated overnight at 4 °C with polyclonal rabbit anti-TRPV1 (NB100–1617; Novus Biologicals, Abingdon, United Kingdom; diluted 1:500 in 4% normal goat serum in PBS) or anti-TRPA1 primary antibodies (ACC-03, Alomone Labs, Jerusalem, Israel; diluted 1:300 in 4% normal goat serum in PBS).

    Techniques: Immunolabeling, Ligation

    TRPA1 and TRPV4 protein levels. Hippocampus lysates were immunoreacted with specific TRPA1 (a) and TRPV4 (b) antibodies. TRPA1, but not TRPV4, increased with KA injection as compared with the PBS group. TRPA1 protein levels were attenuated by electroacupuncture (EA) at EAR (auricular) as compared with the KA-induced groups. Serious results were not observed in ST36 (ST36-ST37) and sham groups. All statistic results were analyzed and plotted as bar chart in (c) and (d).

    Journal: Mediators of Inflammation

    Article Title: Auricular Electroacupuncture Reduced Inflammation-Related Epilepsy Accompanied by Altered TRPA1, pPKCα, pPKCε, and pERk1/2 Signaling Pathways in Kainic Acid-Treated Rats

    doi: 10.1155/2014/493480

    Figure Lengend Snippet: TRPA1 and TRPV4 protein levels. Hippocampus lysates were immunoreacted with specific TRPA1 (a) and TRPV4 (b) antibodies. TRPA1, but not TRPV4, increased with KA injection as compared with the PBS group. TRPA1 protein levels were attenuated by electroacupuncture (EA) at EAR (auricular) as compared with the KA-induced groups. Serious results were not observed in ST36 (ST36-ST37) and sham groups. All statistic results were analyzed and plotted as bar chart in (c) and (d).

    Article Snippet: The membrane was blocked using 5% nonfat milk in a TBS-T buffer (10 mM Tris, pH 7.5, 100 mM NaCl, and 0.1% Tween 20), incubated with anti-TRPA1 (1 : 1000, Alomone Labs, Jerusalem, Israel), TRPV4 (1 : 1000, Alomone Labs), PKCα (pSer657) (1 : 1000, Millipore, Billerica, MA, USA), PKCε (1 : 500, Novus Biologicals, Littleton, CO, USA), and pERK1/2 (pThr202, pTyr204) (1 : 500, Novus Biologicals, Littleton, CO, USA) in TBS-T containing 1% bovine serum albumin, and incubated for 1 hour at room temperature.

    Techniques: Injection

    Hypoxia induced activation of trigeminal ganglion neurons. (A) Experimental design. TRPA1-KO mice and WT mice were exposed to mild (13% O 2 ) or severe (10% O 2 ) hypoxia for 3 min, quickly euthanized, and then the trigeminal ganglion was sampled. Room air (21% O 2 ) was used as the control. (B) Typical example from WT mice that experienced 13% hypoxia. Note that p-ERK was positive in both neurons (arrows) and satellite cells (open triangles). (C) Ratio of p-ERK and NeuN double positive cells out of the total NeuN positive population. Each column represents mean and SEM in five animals. Two-way ANOVA revealed that there was a significant difference among O 2 concentrations ( F 2, 24 = 12.67, p = 0.0002) and interaction between O 2 concentration and genotype ( F 2, 24 = 4.882, p = 0.0166). Values of p in the figure were calculated by Sidak’s multiple comparison test.

    Journal: Frontiers in Physiology

    Article Title: Transient Receptor Potential Ankyrin 1 Mediates Hypoxic Responses in Mice

    doi: 10.3389/fphys.2020.576209

    Figure Lengend Snippet: Hypoxia induced activation of trigeminal ganglion neurons. (A) Experimental design. TRPA1-KO mice and WT mice were exposed to mild (13% O 2 ) or severe (10% O 2 ) hypoxia for 3 min, quickly euthanized, and then the trigeminal ganglion was sampled. Room air (21% O 2 ) was used as the control. (B) Typical example from WT mice that experienced 13% hypoxia. Note that p-ERK was positive in both neurons (arrows) and satellite cells (open triangles). (C) Ratio of p-ERK and NeuN double positive cells out of the total NeuN positive population. Each column represents mean and SEM in five animals. Two-way ANOVA revealed that there was a significant difference among O 2 concentrations ( F 2, 24 = 12.67, p = 0.0002) and interaction between O 2 concentration and genotype ( F 2, 24 = 4.882, p = 0.0166). Values of p in the figure were calculated by Sidak’s multiple comparison test.

    Article Snippet: Specificity of the anti-TRPA1 antibody was examined by pre-mixing with an excess amount of antigen peptide (weight ratio 1:1, which corresponds molar ratio of ~1:100, Alomone) for 60 min.

    Techniques: Activation Assay, Mouse Assay, Concentration Assay

    Effect of genetic and pharmacological inhibition of TRPA1 on respiratory chemoreflex. (A) Representative tracing of pressure signals in whole body plethysmography. Respiration of WT and KO mice was measured using flow-through type whole body plethysmography. Every gas condition was maintained for 3 min, and the data were collected during the last 20-s period. Each stimulus was separated by intervals of 20 min or more of normal room air. Plethysmographic signal that is a pressure difference between the measuring chamber and the reference chamber, and O 2 concentration in the measuring chamber were continuously monitored. Data for baseline and recovery periods were obtained during the last 3 min before the next stimulation. (B) Group data obtained in whole body plethysmography. Data are shown as mean ± SEM. n = 8 for WT and n = 8 for KO mice. Two-way ANOVA revealed that there was a significant difference among gas conditions ( F 8, 112 = 101.7, p

    Journal: Frontiers in Physiology

    Article Title: Transient Receptor Potential Ankyrin 1 Mediates Hypoxic Responses in Mice

    doi: 10.3389/fphys.2020.576209

    Figure Lengend Snippet: Effect of genetic and pharmacological inhibition of TRPA1 on respiratory chemoreflex. (A) Representative tracing of pressure signals in whole body plethysmography. Respiration of WT and KO mice was measured using flow-through type whole body plethysmography. Every gas condition was maintained for 3 min, and the data were collected during the last 20-s period. Each stimulus was separated by intervals of 20 min or more of normal room air. Plethysmographic signal that is a pressure difference between the measuring chamber and the reference chamber, and O 2 concentration in the measuring chamber were continuously monitored. Data for baseline and recovery periods were obtained during the last 3 min before the next stimulation. (B) Group data obtained in whole body plethysmography. Data are shown as mean ± SEM. n = 8 for WT and n = 8 for KO mice. Two-way ANOVA revealed that there was a significant difference among gas conditions ( F 8, 112 = 101.7, p

    Article Snippet: Specificity of the anti-TRPA1 antibody was examined by pre-mixing with an excess amount of antigen peptide (weight ratio 1:1, which corresponds molar ratio of ~1:100, Alomone) for 60 min.

    Techniques: Inhibition, Mouse Assay, Concentration Assay

    Distribution of Transient receptor potential ankyrin 1 (TRPA1) immunoreactivity in the nasal cavity of mouse. (A) Schematic representation of the lateral view of the mouse nasal area showing three levels of the coronal sections examined. (B) Photomicrographs taken from the areas indicated by squares (a–g) in (A) of a representative mouse. Arrows indicate TRPA1-positive structures (see text). (d’) was taken from a similar region to (d) and treated with a mixture of anti-TRPA1 antibody and an excess amount of antigen peptide. Similar results were obtained in three animals. et, ethmoturbinate; ms, maxillary sinus; mt, maxilloturbinate; nt, nasoturbinate; OB, olfactory bulb; ri, root of incisor tooth; se, septum; vn, vomeronasal organ.

    Journal: Frontiers in Physiology

    Article Title: Transient Receptor Potential Ankyrin 1 Mediates Hypoxic Responses in Mice

    doi: 10.3389/fphys.2020.576209

    Figure Lengend Snippet: Distribution of Transient receptor potential ankyrin 1 (TRPA1) immunoreactivity in the nasal cavity of mouse. (A) Schematic representation of the lateral view of the mouse nasal area showing three levels of the coronal sections examined. (B) Photomicrographs taken from the areas indicated by squares (a–g) in (A) of a representative mouse. Arrows indicate TRPA1-positive structures (see text). (d’) was taken from a similar region to (d) and treated with a mixture of anti-TRPA1 antibody and an excess amount of antigen peptide. Similar results were obtained in three animals. et, ethmoturbinate; ms, maxillary sinus; mt, maxilloturbinate; nt, nasoturbinate; OB, olfactory bulb; ri, root of incisor tooth; se, septum; vn, vomeronasal organ.

    Article Snippet: Specificity of the anti-TRPA1 antibody was examined by pre-mixing with an excess amount of antigen peptide (weight ratio 1:1, which corresponds molar ratio of ~1:100, Alomone) for 60 min.

    Techniques:

    RNA expression of TRPA1 was undetectable in the mouse carotid body. RNAs are subjected to RT-PCR from the mouse carotid body and that from dorsal root ganglia (DRG) as a positive control of TRPA1 expression. Identification of carotid body is confirmed by the detection of tyrosine hydroxylase (TH) RNA, a specific marker for carotid body in the carotid artery region. A similar result was obtained in two mice.

    Journal: Frontiers in Physiology

    Article Title: Transient Receptor Potential Ankyrin 1 Mediates Hypoxic Responses in Mice

    doi: 10.3389/fphys.2020.576209

    Figure Lengend Snippet: RNA expression of TRPA1 was undetectable in the mouse carotid body. RNAs are subjected to RT-PCR from the mouse carotid body and that from dorsal root ganglia (DRG) as a positive control of TRPA1 expression. Identification of carotid body is confirmed by the detection of tyrosine hydroxylase (TH) RNA, a specific marker for carotid body in the carotid artery region. A similar result was obtained in two mice.

    Article Snippet: Specificity of the anti-TRPA1 antibody was examined by pre-mixing with an excess amount of antigen peptide (weight ratio 1:1, which corresponds molar ratio of ~1:100, Alomone) for 60 min.

    Techniques: RNA Expression, Reverse Transcription Polymerase Chain Reaction, Positive Control, Expressing, Marker, Mouse Assay