trizol reagent invitrogen thermo fisher scientific inc  (Thermo Fisher)


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    Structured Review

    Thermo Fisher trizol reagent invitrogen thermo fisher scientific inc
    Trizol Reagent Invitrogen Thermo Fisher Scientific Inc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trizol reagent invitrogen thermo fisher scientific inc/product/Thermo Fisher
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    trizol reagent invitrogen thermo fisher scientific inc - by Bioz Stars, 2019-10
    88/100 stars

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    Related Articles

    Amplification:

    Article Title: Kobophenol A Isolated from Roots of Caragana sinica (Buc’hoz) Rehder Exhibits Anti-inflammatory Activity by Regulating NF-κB Nuclear Translocation in J774A.1 Cells
    Article Snippet: 2.7 J774 A.1 macrophage cells were seeded in 6-well plates at a density of 4 × 106 cells/well and pre-treated with KPA (25–100 μM) for 1 h and then stimulated 1 μg/ml LPS for 24 h. Total RNA was extracted by Trizol reagent (Invitrogen, Thermo Fisher Scientific, Inc.) according to the manufacturer's recommended instructions. .. 2.7 J774 A.1 macrophage cells were seeded in 6-well plates at a density of 4 × 106 cells/well and pre-treated with KPA (25–100 μM) for 1 h and then stimulated 1 μg/ml LPS for 24 h. Total RNA was extracted by Trizol reagent (Invitrogen, Thermo Fisher Scientific, Inc.) according to the manufacturer's recommended instructions.

    Article Title: Kobophenol A Isolated from Roots of Caragana sinica (Buc’hoz) Rehder Exhibits Anti-inflammatory Activity by Regulating NF-κB Nuclear Translocation in J774A.1 Cells
    Article Snippet: J774 A.1 macrophage cells were seeded in 6-well plates at a density of 4 × 106 cells/well and pre-treated with KPA (25–100 μM) for 1 h and then stimulated 1 μg/ml LPS for 24 h. Total RNA was extracted by Trizol reagent (Invitrogen, Thermo Fisher Scientific, Inc.) according to the manufacturer's recommended instructions. .. J774 A.1 macrophage cells were seeded in 6-well plates at a density of 4 × 106 cells/well and pre-treated with KPA (25–100 μM) for 1 h and then stimulated 1 μg/ml LPS for 24 h. Total RNA was extracted by Trizol reagent (Invitrogen, Thermo Fisher Scientific, Inc.) according to the manufacturer's recommended instructions.

    Agarose Gel Electrophoresis:

    Article Title: Kobophenol A Isolated from Roots of Caragana sinica (Buc’hoz) Rehder Exhibits Anti-inflammatory Activity by Regulating NF-κB Nuclear Translocation in J774A.1 Cells
    Article Snippet: 2.7 J774 A.1 macrophage cells were seeded in 6-well plates at a density of 4 × 106 cells/well and pre-treated with KPA (25–100 μM) for 1 h and then stimulated 1 μg/ml LPS for 24 h. Total RNA was extracted by Trizol reagent (Invitrogen, Thermo Fisher Scientific, Inc.) according to the manufacturer's recommended instructions. .. PCR cycling conditions were 5 min at 95 °C followed by 25 cycles of 30 sec at 95 °C, 40 sec at between 50 and 60 °C, 1 min at 72 °C and final extension for 10 min at 72 °C.

    Article Title: Kobophenol A Isolated from Roots of Caragana sinica (Buc’hoz) Rehder Exhibits Anti-inflammatory Activity by Regulating NF-κB Nuclear Translocation in J774A.1 Cells
    Article Snippet: J774 A.1 macrophage cells were seeded in 6-well plates at a density of 4 × 106 cells/well and pre-treated with KPA (25–100 μM) for 1 h and then stimulated 1 μg/ml LPS for 24 h. Total RNA was extracted by Trizol reagent (Invitrogen, Thermo Fisher Scientific, Inc.) according to the manufacturer's recommended instructions. .. PCR cycling conditions were 5 min at 95 °C followed by 25 cycles of 30 sec at 95 °C, 40 sec at between 50 and 60 °C, 1 min at 72 °C and final extension for 10 min at 72 °C.

    Size-exclusion Chromatography:

    Article Title: Kobophenol A Isolated from Roots of Caragana sinica (Buc’hoz) Rehder Exhibits Anti-inflammatory Activity by Regulating NF-κB Nuclear Translocation in J774A.1 Cells
    Article Snippet: 2.7 J774 A.1 macrophage cells were seeded in 6-well plates at a density of 4 × 106 cells/well and pre-treated with KPA (25–100 μM) for 1 h and then stimulated 1 μg/ml LPS for 24 h. Total RNA was extracted by Trizol reagent (Invitrogen, Thermo Fisher Scientific, Inc.) according to the manufacturer's recommended instructions. .. The cDNA was amplified using specific primers and AccuPower® Pfu PCR premix (Bioneer Corporation, Daejeon, Republic of Korea) in MyGene™ Series Peltier Thermal Cycler Model MG96 G (LongGene Scientific Instruments, Hangzhou, China).

    Article Title: Kobophenol A Isolated from Roots of Caragana sinica (Buc’hoz) Rehder Exhibits Anti-inflammatory Activity by Regulating NF-κB Nuclear Translocation in J774A.1 Cells
    Article Snippet: J774 A.1 macrophage cells were seeded in 6-well plates at a density of 4 × 106 cells/well and pre-treated with KPA (25–100 μM) for 1 h and then stimulated 1 μg/ml LPS for 24 h. Total RNA was extracted by Trizol reagent (Invitrogen, Thermo Fisher Scientific, Inc.) according to the manufacturer's recommended instructions. .. The cDNA was amplified using specific primers and AccuPower® Pfu PCR premix (Bioneer Corporation, Daejeon, Republic of Korea) in MyGene™ Series Peltier Thermal Cycler Model MG96 G (LongGene Scientific Instruments, Hangzhou, China).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Kobophenol A Isolated from Roots of Caragana sinica (Buc’hoz) Rehder Exhibits Anti-inflammatory Activity by Regulating NF-κB Nuclear Translocation in J774A.1 Cells
    Article Snippet: Paragraph title: Reverse transcription-polymerase chain reaction (RT-PCR) ... 2.7 J774 A.1 macrophage cells were seeded in 6-well plates at a density of 4 × 106 cells/well and pre-treated with KPA (25–100 μM) for 1 h and then stimulated 1 μg/ml LPS for 24 h. Total RNA was extracted by Trizol reagent (Invitrogen, Thermo Fisher Scientific, Inc.) according to the manufacturer's recommended instructions.

    Polymerase Chain Reaction:

    Article Title: Kobophenol A Isolated from Roots of Caragana sinica (Buc’hoz) Rehder Exhibits Anti-inflammatory Activity by Regulating NF-κB Nuclear Translocation in J774A.1 Cells
    Article Snippet: 2.7 J774 A.1 macrophage cells were seeded in 6-well plates at a density of 4 × 106 cells/well and pre-treated with KPA (25–100 μM) for 1 h and then stimulated 1 μg/ml LPS for 24 h. Total RNA was extracted by Trizol reagent (Invitrogen, Thermo Fisher Scientific, Inc.) according to the manufacturer's recommended instructions. .. 2.7 J774 A.1 macrophage cells were seeded in 6-well plates at a density of 4 × 106 cells/well and pre-treated with KPA (25–100 μM) for 1 h and then stimulated 1 μg/ml LPS for 24 h. Total RNA was extracted by Trizol reagent (Invitrogen, Thermo Fisher Scientific, Inc.) according to the manufacturer's recommended instructions.

    Article Title: Kobophenol A Isolated from Roots of Caragana sinica (Buc’hoz) Rehder Exhibits Anti-inflammatory Activity by Regulating NF-κB Nuclear Translocation in J774A.1 Cells
    Article Snippet: J774 A.1 macrophage cells were seeded in 6-well plates at a density of 4 × 106 cells/well and pre-treated with KPA (25–100 μM) for 1 h and then stimulated 1 μg/ml LPS for 24 h. Total RNA was extracted by Trizol reagent (Invitrogen, Thermo Fisher Scientific, Inc.) according to the manufacturer's recommended instructions. .. J774 A.1 macrophage cells were seeded in 6-well plates at a density of 4 × 106 cells/well and pre-treated with KPA (25–100 μM) for 1 h and then stimulated 1 μg/ml LPS for 24 h. Total RNA was extracted by Trizol reagent (Invitrogen, Thermo Fisher Scientific, Inc.) according to the manufacturer's recommended instructions.

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  • 99
    Thermo Fisher trizol ls
    Trizol Ls, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trizol ls/product/Thermo Fisher
    Average 99 stars, based on 103 article reviews
    Price from $9.99 to $1999.99
    trizol ls - by Bioz Stars, 2019-10
    99/100 stars
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