trizol reagent invitrogen thermo fisher scientific inc  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    TRIzol Reagent
    Description:
    TRIzol Reagent is a complete ready to use reagent for the isolation of high quality total RNA or the simultaneous isolation of RNA DNA and protein from a variety of biological samples This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA DNA and proteins from cell and tissue samples of human animal plant yeast or bacterial origin within one hour Key features of TRIzol Reagent include • Permits the isolation of RNA DNA and protein from the same sample• Offers superior lysis capability even with difficult sample types• Optimized formulations and protocols for tissues cells serum virus and bacteriaReliably purify RNA from multiple sample volumes and sourcesTRIzol Reagent performs well with small quantities of tissue 50 100 mg and cells 5 × 106 as well as with large quantities of tissue 1 g and cells 107 and comes with prototcols for purification from samples of human animal plant or bacterial origin TRIzol Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization The simplicity of the TRIzol Reagent method allows simultaneous processing of a large number of samples The entire procedure can be completed in 1 hour Total RNA isolated by TRIzol Reagent is free of protein and DNA contamination Formulated for isolation of multiple molecular targetsTRIzol Reagent allows you to perform sequential precipitation of RNA DNA and proteins from a single sample After homogenizing the sample with TRIzol Reagent chloroform is added and the homogenate is allowed to separate into a clear upper aqueous layer containing RNA and interphase and red lower organic layers containing the DNA and proteins RNA is precipitated from the aqueous layer with isopropanol DNA is precipitated from the interphase organic layer with ethanol Protein is precipitated from the phenol ethanol supernatant by isopropanol precipitation The precipitated RNA DNA or protein is washed to remove impurities and then resuspended for use in downstream applications
    Catalog Number:
    15596018
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|RNAi, Epigenetics & Non-Coding RNA Research|RNA Extraction|Total RNA Isolation|Total RNA from Animal Cells & Tissues|Total RNA from Bacterial Samples|Total RNA from Plant Cells|Total RNA from Yeast|miRNA Isolation|miRNA & Non-Coding RNA Analysis
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher trizol reagent invitrogen thermo fisher scientific inc
    TRIzol Reagent is a complete ready to use reagent for the isolation of high quality total RNA or the simultaneous isolation of RNA DNA and protein from a variety of biological samples This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA DNA and proteins from cell and tissue samples of human animal plant yeast or bacterial origin within one hour Key features of TRIzol Reagent include • Permits the isolation of RNA DNA and protein from the same sample• Offers superior lysis capability even with difficult sample types• Optimized formulations and protocols for tissues cells serum virus and bacteriaReliably purify RNA from multiple sample volumes and sourcesTRIzol Reagent performs well with small quantities of tissue 50 100 mg and cells 5 × 106 as well as with large quantities of tissue 1 g and cells 107 and comes with prototcols for purification from samples of human animal plant or bacterial origin TRIzol Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization The simplicity of the TRIzol Reagent method allows simultaneous processing of a large number of samples The entire procedure can be completed in 1 hour Total RNA isolated by TRIzol Reagent is free of protein and DNA contamination Formulated for isolation of multiple molecular targetsTRIzol Reagent allows you to perform sequential precipitation of RNA DNA and proteins from a single sample After homogenizing the sample with TRIzol Reagent chloroform is added and the homogenate is allowed to separate into a clear upper aqueous layer containing RNA and interphase and red lower organic layers containing the DNA and proteins RNA is precipitated from the aqueous layer with isopropanol DNA is precipitated from the interphase organic layer with ethanol Protein is precipitated from the phenol ethanol supernatant by isopropanol precipitation The precipitated RNA DNA or protein is washed to remove impurities and then resuspended for use in downstream applications
    https://www.bioz.com/result/trizol reagent invitrogen thermo fisher scientific inc/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    trizol reagent invitrogen thermo fisher scientific inc - by Bioz Stars, 2020-05
    99/100 stars

    Images

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Clinicopathologic Significance of HIF-1α, CXCR4, and VEGF Expression in Colon Cancer
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions, and reverse transcribed. mRNA expression of HIF-1α , CXCR4, VEGF, and β actin was determined by real-time PCR using SYBR Green master mix (ABI, Foster City, CA). ..

    Article Title: Long Non Coding RNA SNHG16 Facilitates Proliferation, Migration, Invasion and Autophagy of Neuroblastoma Cells via Sponging miR-542-3p and Upregulating ATG5 Expression
    Article Snippet: .. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) TRIzol reagent (Invitrogen) was employed to isolate total RNA from paired NB tissues or cells. .. The SuperScript Reverse Transcriptase Kit (Vazyme, Nanjing, China) was used to generate cDNA for SNHG16, miR-542-3p and ATG5.

    RNA Extraction:

    Article Title: High-quality RNA extraction from the sea urchin Paracentrotus lividus embryos
    Article Snippet: .. RNA extraction Five different methods of RNA extraction were compared. i) TRIzol® RNA extraction method Total RNA was extracted using TRIzol (Invitrogen, Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s instructions, homogenizing with TissueLyser (Qiagen, Austin, TX, US) with 3 mm sterile aluminium beads at 20.1 Hertz (Hz) for 5 minutes. ..

    Article Title: Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine
    Article Snippet: .. Real-time and end-point RT-PCR Cells were seeded in 5 cm-well plates (3 × 105 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 24 h. Cells were washed 3X with PBS and treated as indicated in medium supplemented with physiological 5 mM glucose and 5% dialyzed serum for 24 h. RNA extraction was performed using TRIZOL® (Life Technologies, Cat # 15596–026) and quantification was determined using a Qubit® 2.0 Fluorometer (Life Technologies, Cat #Q32866) with a Qubit™ RNA Assay Kit (Life Technologies, Cat # Q32852). .. The cDNA was prepared with the ProtoScript® First Strand cDNA Synthesis Kit (New England BioLabs, Cat # E6300S) using oligo dT as a primer.

    Isolation:

    Article Title: Phosphorylation Controls the Localization and Activation of the Lumenal Carbonic Anhydrase in Chlamydomonas reinhardtii
    Article Snippet: .. Total RNA was isolated from 2 ml culture by using the Trizol™ reagent according to the manufacturers protocol (Life technologies, US). .. 2,5 ng total RNA from wild-type and mutant cia3 were preheated to 70°C for 20 minutes prior to the RT-PCR reactions.

    Article Title: Plasma vesicle miRNAs for therapy response monitoring in Hodgkin lymphoma patients
    Article Snippet: .. Total RNA was isolated using TRIzol (Thermo Fisher Scientific) according to the manufacturer’s instructions, with some modifications. .. 0.75 ml TRIzol was added to 0.25 ml SEC fractions, mixed properly, and incubated at room temperature for 15 minutes.

    Article Title: Clinicopathologic Significance of HIF-1α, CXCR4, and VEGF Expression in Colon Cancer
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions, and reverse transcribed. mRNA expression of HIF-1α , CXCR4, VEGF, and β actin was determined by real-time PCR using SYBR Green master mix (ABI, Foster City, CA). ..

    Quantitative RT-PCR:

    Article Title: Long Non Coding RNA SNHG16 Facilitates Proliferation, Migration, Invasion and Autophagy of Neuroblastoma Cells via Sponging miR-542-3p and Upregulating ATG5 Expression
    Article Snippet: .. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) TRIzol reagent (Invitrogen) was employed to isolate total RNA from paired NB tissues or cells. .. The SuperScript Reverse Transcriptase Kit (Vazyme, Nanjing, China) was used to generate cDNA for SNHG16, miR-542-3p and ATG5.

    SYBR Green Assay:

    Article Title: Clinicopathologic Significance of HIF-1α, CXCR4, and VEGF Expression in Colon Cancer
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions, and reverse transcribed. mRNA expression of HIF-1α , CXCR4, VEGF, and β actin was determined by real-time PCR using SYBR Green master mix (ABI, Foster City, CA). ..

    Expressing:

    Article Title: Clinicopathologic Significance of HIF-1α, CXCR4, and VEGF Expression in Colon Cancer
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions, and reverse transcribed. mRNA expression of HIF-1α , CXCR4, VEGF, and β actin was determined by real-time PCR using SYBR Green master mix (ABI, Foster City, CA). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine
    Article Snippet: .. Real-time and end-point RT-PCR Cells were seeded in 5 cm-well plates (3 × 105 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 24 h. Cells were washed 3X with PBS and treated as indicated in medium supplemented with physiological 5 mM glucose and 5% dialyzed serum for 24 h. RNA extraction was performed using TRIZOL® (Life Technologies, Cat # 15596–026) and quantification was determined using a Qubit® 2.0 Fluorometer (Life Technologies, Cat #Q32866) with a Qubit™ RNA Assay Kit (Life Technologies, Cat # Q32852). .. The cDNA was prepared with the ProtoScript® First Strand cDNA Synthesis Kit (New England BioLabs, Cat # E6300S) using oligo dT as a primer.

    Lysis:

    Article Title: Integrative analyses of transcriptome sequencing identify novel functional lncRNAs in esophageal squamous cell carcinoma
    Article Snippet: .. The next day, the magnetic bead complexes were subjected to extreme washing with lysis buffer and RNA was extracted with TRIzol (Life Technologies). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher invitrogen trizol reagent
    Invitrogen Trizol Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 44805 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/invitrogen trizol reagent/product/Thermo Fisher
    Average 99 stars, based on 44805 article reviews
    Price from $9.99 to $1999.99
    invitrogen trizol reagent - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    Image Search Results