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Cell Signaling Technology Inc triton x 100 solution
Triton X 100 Solution, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triton x 100 solution/product/Cell Signaling Technology Inc
Average 99 stars, based on 4 article reviews
Price from $9.99 to $1999.99
triton x 100 solution - by Bioz Stars, 2020-09
99/100 stars

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Centrifugation:

Article Title: Decitabine attenuates dextran sodium sulfate-induced ulcerative colitis through regulation of immune regulatory cells and intestinal barrier
Article Snippet: .. Subsequently, 1 ml of a 3:1 permeabilization reagent (cat. no. 00-5523-00; eBioscience; Thermo Fisher Scientific, Inc.) was added, and the cells were incubated at 4°C for 1 h in the dark, followed by the addition of cell permeabilization buffer (2 ml; cat. no. 39487s; Cell Signaling Technology, Inc.), centrifugation twice, and the resuspension of cells to a volume of 100 μl. .. PE-rat anti-mouse Foxp3 (cat. no. 72-5775-40; eBioscience; Thermo Fisher Scientific, Inc.) in 2.5% BSA was added and incubated for 1 h at 4°C in the dark.

Labeling:

Article Title: Prolonged seizure activity causes caspase dependent cleavage and dysfunction of G-protein activated inwardly rectifying potassium channels
Article Snippet: .. After fixation and permeabilization with 0.2% Triton X-100 in PBS for 10 min, total HA-GIRK2A, HA-GIRK1, or GIRK2A proteins were labeled with anti-HA or anti-GIRK2 antibodies (1:300, Cell Signaling), followed by Alexa594-, Alexa488-, or Alexa680-conjugated secondary antibodies, respectively. .. High-resolution gray scale images of immunostained cells were acquired using the same exposure time under a Zeiss Axiovert 200 M inverted microscope equipped with AxioCam HRm Camera and Axiovert software.

Concentration Assay:

Article Title: Conformational Stability and Pathogenic Misfolding of the Integral Membrane Protein PMP22
Article Snippet: .. The cells were then suspended in 100 μL of the permeabilization solution, and an Alexa Fluor 647-labeled monoclonal anti-myc antibody (clone 9E10) (Cell Signaling Technology, Danvers, MA) was added to the solution to a final concentration of 0.75 μg/mL to label intracellular PMP22. .. After a 30 min incubation in the dark at room temperature, the cells were again rinsed and pelleted by centrifugation twice using rinse solution then resuspended in 300 μL of the rinse solution prior to FACS analysis.

Incubation:

Article Title: Impaired Latent Inhibition in GDNF-Deficient Mice Exposed to Chronic Stress
Article Snippet: .. Free-floating brain sections (50 μm) were incubated with a blocking and permeabilization solution (5% donkey serum, 0.3% Triton X-100 in PBS) for 2 h and then incubated overnight at 4°C with the c-Fos primary antibody (Cell Signaling Technologies, 1:300 dilution). .. The sections were rinsed in PBS, 0.1% Tween-20 and incubated for 2 h with Alexa488-conjugated donkey anti rabbit secondary antibody and NeuroTrace 530/615 (Fisher Scientific/Invitrogen, Carlsbad, CA, USA).

Article Title: IL-17 aggravates renal injury by promoting podocyte injury in children with primary nephrotic syndrome
Article Snippet: .. Slides with cells were incubated in culture plates and washed with PBS (3x3 min), followed by fixation with 4% paraformaldehyde for 15 min at room temperature and permeabilization with 0.5% Triton X-100 dissolved in PBS. .. Normal 5% goat serum (cat. no. C0265; Beyotime Institute of Biotechnology) was then added onto the slides for blocking at room temperature for 30 min before Anti-PODXL antibody [EPR9518] (cat. no. ab150358; 1:1,000; Abcam) was added into slides and incubated in a wet box overnight at 4˚C.

Blocking Assay:

Article Title: Impaired Latent Inhibition in GDNF-Deficient Mice Exposed to Chronic Stress
Article Snippet: .. Free-floating brain sections (50 μm) were incubated with a blocking and permeabilization solution (5% donkey serum, 0.3% Triton X-100 in PBS) for 2 h and then incubated overnight at 4°C with the c-Fos primary antibody (Cell Signaling Technologies, 1:300 dilution). .. The sections were rinsed in PBS, 0.1% Tween-20 and incubated for 2 h with Alexa488-conjugated donkey anti rabbit secondary antibody and NeuroTrace 530/615 (Fisher Scientific/Invitrogen, Carlsbad, CA, USA).

Staining:

Article Title: Simkania negevensis may produce long-lasting infections in human pneumocytes and endometrial cells
Article Snippet: .. Following a 1-h permeabilization in a 0.3% Triton X-100 5% FCS PBS solution, the Golgi apparatus and the endoplasmic reticulum were stained using the organelle localization kit containing specific markers, RCAS1 and PDI, respectively (Cell Signaling Technology Inc., Danvers, USA) or the mouse anti-GM130 (Molecular Probes, Thermo Fisher Scientific) according to the manufacturer's instructions. .. Subsequent staining with mouse or rabbit polyclonal anti-S. negevensis antibodies was performed as described earlier, prior to incubation with secondary antibodies (Alexa Fluor 488 goat anti-mouse (1:1000) and Alexa Fluor 594 donkey anti-rabbit (1:1000) or Alexa Fluor 488 donkey anti-rabbit (1:1000) and Alexa Fluor 594 goat anti-mouse (1:1000); Molecular Probes, Thermo Fisher Scientific) and 4΄,6-diamidino-2-phénylindole (DAPI) (1:1000) performed as previously described (Rusconi, Kebbi-Beghdadi and Greub ).

Article Title: Rictor positively regulates B cell receptor signaling by modulating actin reorganization via ezrin
Article Snippet: .. After fixation and permeabilization, the cells were stained for pRictor (T1135, Cell Signaling Technology), pY, pBtk, pSHIP, and pEzrin (T558, Cell Signaling Technology) and analyzed using CFm. .. For flow cytometric analyses, cell suspensions from BM and spleen were incubated with Fcγ receptor (FcγR) blocking Abs (anti-mouse CD16/CD32; BD Bioscience) on ice and stained at optimal dilutions of conjugated Abs in PBS supplemented with 1% FBS.

Chloramphenicol Acetyltransferase Assay:

Article Title: LipiD-QuanT: a novel method to quantify lipid accumulation in live cells [S]
Article Snippet: .. The cells were fixed on coverslips with 1 ml of 4% paraformaldehyde for 20 min in 6-well plates and washed with 1× PBS twice before and after permeabilization in Triton X-100 solution (0.2% Triton X-100 in PBS with 10% goat serum, cat5425; Cell Signaling Technology) for 10 min. .. Samples were blocked for 20 min in 10% goat serum, 5% FCS, and 0.5% BSA in PBS and stained with anti-Perilipin (D1D8) XP® Rabbit mAb (cat#9349S; Cell Signaling Technology) overnight at 4°C as per the manufacturer’s recommendations.

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    Cell Signaling Technology Inc pbs t solution
    Pbs T Solution, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs t solution/product/Cell Signaling Technology Inc
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs t solution - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    90
    Cell Signaling Technology Inc triton x 100 solution
    Triton X 100 Solution, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triton x 100 solution/product/Cell Signaling Technology Inc
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    triton x 100 solution - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

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