tris hcl  (Valiant)

 
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  • 94
    Name:
    Tris hydrochloride reagent grade 99
    Description:
    Tris Hydrochloride
    Catalog Number:
    02103130-cf
    Price:
    10.0
    Category:
    Life Sciences Biochemicals Ultra Pure Buffer Reagents
    Applications:
    Biological buffer, Apoptosis
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    Structured Review

    Valiant tris hcl
    Tris hydrochloride reagent grade 99
    Tris Hydrochloride
    https://www.bioz.com/result/tris hcl/product/Valiant
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris hcl - by Bioz Stars, 2021-03
    94/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Comparison of the gut microbial community between obese and lean peoples using 16S gene sequencing in a Japanese population
    Article Snippet: .. DNA extraction DNA was extracted from fecal samples according to a previously described method. The fecal samples were suspended in a buffer containing 4 M guanidium thiocyanate, 100 mM Tris-HCl (pH 9.0) and 40 mM EDTA and beaten in the presence of zirconia beads using the FastPrep FP100A Instrument (MP Biomedicals, Irvine, CA). ..

    Western Blot:

    Article Title: Inhibition of Prostate Smooth Muscle Contraction by Inhibitors of Polo-Like Kinases
    Article Snippet: Results were expressed using the ΔΔCP method, where number of cycles (Ct) at which the fluorescence signal exceeded a defined threshold for GAPDH was subtracted from Ct values for PLK (CtPLK -CtGAPDH = ΔCP), and values were calculated as 2ˆ- ΔCP and normalized to each other. .. Western Blot Analysis Frozen prostate tissues were homogenized in a buffer containing 25 mM Tris/HCl, 10 μM phenylmethanesulfonyl fluoride, 1 mM benzamidine, and 10 μg/ml leupeptine hemisulfate, using the FastPrep® -24 system with matrix A (MP Biomedicals, Illkirch, France). .. After centrifugation (20,000 g , 4 min), supernatants were assayed for protein concentration using the Dc-Assay kit (Biorad, Munich, Germany) and boiled for 10 min with sodium dodecyl sulfate (SDS) sample buffer (Roth, Karlsruhe, Germany).

    Binding Assay:

    Article Title: The 2-Cys Peroxiredoxin Alkyl Hydroperoxide Reductase C Binds Heme and Participates in Its Intracellular Availability in Streptococcus agalactiae *
    Article Snippet: E. coli BL21 (DE3) transformed with pHis-AhpC was grown to A 600 = 0.6, and expression was induced with 1 m m isopropyl 1-thio-β- d -galactopyranoside for 2 h at 37 °C. .. Cells were pelleted at 3,500 × g for 10 min and then resuspended in 50 m m Tris-HCl, pH 8.0, 300 m m NaCl, containing 20 m m imidazole (binding buffer), and disrupted with glass beads (Fastprep, MP Biomedicals). .. Cell debris was removed by centrifugation at 18,000 × g for 15 min at 4 °C.

    Stripping Membranes:

    Article Title: CHARACTERIZATION OF THE CARBOHYDRATE COMPONENTS OF Taenia solium ONCOSPHERE PROTEINS AND THEIR ROLE IN THE ANTIGENICITY
    Article Snippet: The strips were blocked with a blocking solution containing 0.2% PBS-T at 4°C overnight. .. After washing, each strip was incubated with a different biotinylated lectin (VECTOR LABORATORIES): Con A (2.5 ug/mL), LCA (20 ug/mL), RCA I (20 ug/mL), SBA (20 ug/mL), UEA I (20 ug/mL), WGA (20 ug/mL), and MAL II (20 ug/mL) in 0.05% PBS-T for 2 h at room temperature with gentle rocking, washed five times, and then incubated for 2 h at room temperature with Streptavidin horseradish peroxidase conjugate (Roche Applied Science, Indianapolis, IN) 1:375 diluted in 0.05% PBS-T. After washing, the strips were incubated in 0.06% diaminobenzidine tetrahydrochloride-DAB (SIGMA) (w/v) in 50 mM of Tris-HCl (MP Biomedicals) (pH 7.6) and 0.1% of H2 O2 (v/v). ..

    Incubation:

    Article Title: CHARACTERIZATION OF THE CARBOHYDRATE COMPONENTS OF Taenia solium ONCOSPHERE PROTEINS AND THEIR ROLE IN THE ANTIGENICITY
    Article Snippet: The strips were blocked with a blocking solution containing 0.2% PBS-T at 4°C overnight. .. After washing, each strip was incubated with a different biotinylated lectin (VECTOR LABORATORIES): Con A (2.5 ug/mL), LCA (20 ug/mL), RCA I (20 ug/mL), SBA (20 ug/mL), UEA I (20 ug/mL), WGA (20 ug/mL), and MAL II (20 ug/mL) in 0.05% PBS-T for 2 h at room temperature with gentle rocking, washed five times, and then incubated for 2 h at room temperature with Streptavidin horseradish peroxidase conjugate (Roche Applied Science, Indianapolis, IN) 1:375 diluted in 0.05% PBS-T. After washing, the strips were incubated in 0.06% diaminobenzidine tetrahydrochloride-DAB (SIGMA) (w/v) in 50 mM of Tris-HCl (MP Biomedicals) (pH 7.6) and 0.1% of H2 O2 (v/v). ..

    Article Title: Functional interactions between Choline kinase ?, epidermal growth factor receptor (EGFR), and c-Src in breast cancer cell proliferations
    Article Snippet: In vitro choline kinase activity was measured as described previously ( ) with minor modifications. .. Aliquots of cell lysate were incubated in the reaction buffer containing 0.1M Tris HCl pH 8.0, 12 mM MgCl2 , 10 mM ATP, [methyl-14 C]-Choline chloride 0.25 mM (MP Biomedicals, Solon, OH), 1% NP-40 for 15 min at 37°C. .. Phosphocholine was isolated using AG 1-x8 Resin (Bio-Rad) as described in , and radioactivity was measured by scintillation counting.

    Whole Genome Amplification:

    Article Title: CHARACTERIZATION OF THE CARBOHYDRATE COMPONENTS OF Taenia solium ONCOSPHERE PROTEINS AND THEIR ROLE IN THE ANTIGENICITY
    Article Snippet: The strips were blocked with a blocking solution containing 0.2% PBS-T at 4°C overnight. .. After washing, each strip was incubated with a different biotinylated lectin (VECTOR LABORATORIES): Con A (2.5 ug/mL), LCA (20 ug/mL), RCA I (20 ug/mL), SBA (20 ug/mL), UEA I (20 ug/mL), WGA (20 ug/mL), and MAL II (20 ug/mL) in 0.05% PBS-T for 2 h at room temperature with gentle rocking, washed five times, and then incubated for 2 h at room temperature with Streptavidin horseradish peroxidase conjugate (Roche Applied Science, Indianapolis, IN) 1:375 diluted in 0.05% PBS-T. After washing, the strips were incubated in 0.06% diaminobenzidine tetrahydrochloride-DAB (SIGMA) (w/v) in 50 mM of Tris-HCl (MP Biomedicals) (pH 7.6) and 0.1% of H2 O2 (v/v). ..

    Centrifugation:

    Article Title: Heterologous expression of human pro-inflammatory Caspase-1 in Saccharomyces cerevisiae and comparison to pro-apoptotic Caspase-8
    Article Snippet: Western blotting assays Western blotting assays were carried out by standard techniques. .. Cells were harvested by centrifugation and disrupted by bead beating with a FastPrep 24 (MP Biomedicals) in 50 mM Tris-HCl pH 7.5 containing 10% glycerol, 0.1% NP-40, 1% Triton X-100, 0.1% sodium dodecyl sulfate (SDS), 150 mM NaCl, 5 mM EDTA, 50 mM NaF, 50 mM glycerol phosphate, 5 mM Na2 P2 O7 , 1 mM sodium orthovanadate, 3 mM Phenylmethylsulfonyl fluoride (PMSF), and Pierce Protease Inhibitor (ThermoFisher). .. Lysates were cleared by centrifugation at 4°C and protein concentrations were determined by measuring the OD280.

    Protease Inhibitor:

    Article Title: Heterologous expression of human pro-inflammatory Caspase-1 in Saccharomyces cerevisiae and comparison to pro-apoptotic Caspase-8
    Article Snippet: Western blotting assays Western blotting assays were carried out by standard techniques. .. Cells were harvested by centrifugation and disrupted by bead beating with a FastPrep 24 (MP Biomedicals) in 50 mM Tris-HCl pH 7.5 containing 10% glycerol, 0.1% NP-40, 1% Triton X-100, 0.1% sodium dodecyl sulfate (SDS), 150 mM NaCl, 5 mM EDTA, 50 mM NaF, 50 mM glycerol phosphate, 5 mM Na2 P2 O7 , 1 mM sodium orthovanadate, 3 mM Phenylmethylsulfonyl fluoride (PMSF), and Pierce Protease Inhibitor (ThermoFisher). .. Lysates were cleared by centrifugation at 4°C and protein concentrations were determined by measuring the OD280.

    Polymerase Chain Reaction:

    Article Title: Mitochondrial control region I and microsatellite analyses of endangered Philippine hornbill species (Aves; Bucerotidae) detect gene flow between island populations and genetic diversity loss
    Article Snippet: The second fragment was amplified with a CRI specific forward primer (Penpan_CRI-spec-128for for P. panini , Penmanman_CRI-spec-115for for P. m. manillae , Penaff_CRI-spec-124for for museum specimen ZMB 21773, and Acewal_CRI-spec-130for for A. waldeni ) and the reverse primer AcePen_644rev or, when amplificates did not yield sufficient results, also with AcePen_426rev (Table ). .. The 37.5 μl PCR reaction volumes (buffer solution: 10mM Tris-HCl, pH 9.0, 50mM KCl, 1.5mM MgCl2 , 0.1% Triton X100, 0.2 mg/ml Bovine Serum Albumin (BSA)) were set up as follows: 6 μl dNTP Mixture (2.0 mM each), 1.2 μl of each primer (10 μM), 3 μl DNA template, 0.25 μl Taq polymerase (5U/μl, MP Biomedicals). ..

    High Performance Liquid Chromatography:

    Article Title: Leishmania major possesses a unique HemG-type protoporphyrinogen IX oxidase
    Article Snippet: Samples were taken every 2 h over 10 h and OD (optical density) was determined photometrically by 578 nm (Ultrospec 500 Pro, Amersham Biosciences). .. HPLC cultures were centrifuged by 2500×g, and cells were mechanically disrupted with glass beads (100 μm) in 50 mM Tris–HCl pH 8.0 containing 2% (v/v) Tween 80 using FastPrep®-24 Instrument (MP Biomedicals). ..

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  • 94
    Valiant m tris hcl
    M Tris Hcl, supplied by Valiant, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris hcl/product/Valiant
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m tris hcl - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

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