tris hcl  (Jena Bioscience)


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  • 86
    Name:
    Tris HCl Buffer 1 M pH 7 5
    Description:

    Catalog Number:
    BU-125L
    Price:
    53.7
    Category:
    Molecular Biology
    Size:
    1 l
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    Structured Review

    Jena Bioscience tris hcl

    https://www.bioz.com/result/tris hcl/product/Jena Bioscience
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris hcl - by Bioz Stars, 2021-06
    86/100 stars

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    Related Articles

    Protease Inhibitor:

    Article Title: Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics
    Article Snippet: From the resultant pellet of the E. coli cell lysate, the ribosomal fraction was isolated via sucrose density gradient ultracentrifugation, as reported previously . .. The ribosomal fraction was suspended in polysome buffer (50 mM Tris-HCl, pH 7.5, 10 mM MgCl2 , and 25 mM KCl), containing an EDTA-free protease inhibitor cocktail (as indicated by the manufacturer), and was then reacted with 2 mM IAM at room temperature for 30 min. After the ribosomal fraction was washed with the polysome buffer, the ribosomes were treated with 5′-biotin-dC-puromycin (Jena Bioscience, Jena, Germany) in TTBS (20 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.6) at 37 °C for 15 min and were then reacted with avidin magnetic beads (Wako Pure Chemical Industries) to finally capture the newly synthesized polypeptides in ribosomes in the E. coli cells in culture. ..

    Avidin-Biotin Assay:

    Article Title: Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics
    Article Snippet: From the resultant pellet of the E. coli cell lysate, the ribosomal fraction was isolated via sucrose density gradient ultracentrifugation, as reported previously . .. The ribosomal fraction was suspended in polysome buffer (50 mM Tris-HCl, pH 7.5, 10 mM MgCl2 , and 25 mM KCl), containing an EDTA-free protease inhibitor cocktail (as indicated by the manufacturer), and was then reacted with 2 mM IAM at room temperature for 30 min. After the ribosomal fraction was washed with the polysome buffer, the ribosomes were treated with 5′-biotin-dC-puromycin (Jena Bioscience, Jena, Germany) in TTBS (20 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.6) at 37 °C for 15 min and were then reacted with avidin magnetic beads (Wako Pure Chemical Industries) to finally capture the newly synthesized polypeptides in ribosomes in the E. coli cells in culture. ..

    Magnetic Beads:

    Article Title: Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics
    Article Snippet: From the resultant pellet of the E. coli cell lysate, the ribosomal fraction was isolated via sucrose density gradient ultracentrifugation, as reported previously . .. The ribosomal fraction was suspended in polysome buffer (50 mM Tris-HCl, pH 7.5, 10 mM MgCl2 , and 25 mM KCl), containing an EDTA-free protease inhibitor cocktail (as indicated by the manufacturer), and was then reacted with 2 mM IAM at room temperature for 30 min. After the ribosomal fraction was washed with the polysome buffer, the ribosomes were treated with 5′-biotin-dC-puromycin (Jena Bioscience, Jena, Germany) in TTBS (20 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.6) at 37 °C for 15 min and were then reacted with avidin magnetic beads (Wako Pure Chemical Industries) to finally capture the newly synthesized polypeptides in ribosomes in the E. coli cells in culture. ..

    Synthesized:

    Article Title: Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics
    Article Snippet: From the resultant pellet of the E. coli cell lysate, the ribosomal fraction was isolated via sucrose density gradient ultracentrifugation, as reported previously . .. The ribosomal fraction was suspended in polysome buffer (50 mM Tris-HCl, pH 7.5, 10 mM MgCl2 , and 25 mM KCl), containing an EDTA-free protease inhibitor cocktail (as indicated by the manufacturer), and was then reacted with 2 mM IAM at room temperature for 30 min. After the ribosomal fraction was washed with the polysome buffer, the ribosomes were treated with 5′-biotin-dC-puromycin (Jena Bioscience, Jena, Germany) in TTBS (20 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.6) at 37 °C for 15 min and were then reacted with avidin magnetic beads (Wako Pure Chemical Industries) to finally capture the newly synthesized polypeptides in ribosomes in the E. coli cells in culture. ..

    Crystallization Assay:

    Article Title: Conformational flexibility revealed by the crystal structure of a crenarchaeal RadA
    Article Snippet: The two major distinct peaks eluted from the column were both analysed by SDS–PAGE and both correspond to Ss RadA. .. Crystallization and X-ray data collection Pure protein was concentrated to 7 mg ml−1 in 20 mM Tris–HCl buffer, pH 7.5 with 250 mM NaCl and screened for crystallization using JenaBiosciences HTS screens in 96-well format sitting drop plates (Greiner). ..

    Binding Assay:

    Article Title: Structural basis for RNA duplex recognition and unwinding by the DEAD-box helicase Mss116p
    Article Snippet: The absorbance of the eluted protein above the background signal of the buffer was measured at 260 and 280 nm, and the change in A260 /A280 signal at increasing concentrations of ATP was fit to a simple one-site ligand-binding model to calculate a K d for the protein-ATP complex. .. ATP-agarose binding assays were performed in 20 mM Tris-HCl (pH 7.5), 100 mM KCl, 10% glycerol, 1 mM DTT, 5 mM MgCl2 by incubating AP-ATP-agarose (150 μl; Jena Bioscience) with ~50 μg of protein overnight at 4 °C with agitation. .. The beads were pelleted by centrifugation (1,000 x g, 1 min), the supernatant was removed, and the pellet was resuspended in binding buffer (150 μl).

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