tris buffered saline replaced millipore assay buffer  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Tris Buffered Saline Solution
    Description:

    Catalog Number:
    sre0032
    Price:
    None
    Applications:
    For use as a wash buffer in western blotting, ELISA, and immunohistochemical (IHC) staining procedures.
    Buy from Supplier


    Structured Review

    Millipore tris buffered saline replaced millipore assay buffer

    https://www.bioz.com/result/tris buffered saline replaced millipore assay buffer/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    tris buffered saline replaced millipore assay buffer - by Bioz Stars, 2020-04
    99/100 stars

    Images

    Related Articles

    Centrifugation:

    Article Title: Domains in the SPT5 Protein That Modulate Its Transcriptional Regulatory Properties
    Article Snippet: After centrifugation at 3,000 rpm for 10 min in a CS-6R Beckman centrifuge, supernatant was applied to an anti-Flag M2 affinity gel column (Sigma) as specified by the manufacturer. .. Flag-tagged fusion proteins were eluted with a Tris-buffered saline solution containing Flag peptide (Sigma) at 100 μg/ml.

    Blocking Assay:

    Article Title: Identification of Sites in Adenovirus Hexon for Foreign Peptide Incorporation
    Article Snippet: .. After extensive washes with 0.05% Tween 20 in Tris-buffered saline (TBS) and blocking with blocking solution (2% bovine serum albumin and 0.05% Tween 20 in TBS), the immobilized viruses were incubated with anti-His tag monoclonal antibody (Chemicon) for 2 h at room temperature, followed by AP-conjugated goat anti-mouse antibody incubation. .. Color reaction was performed with p -nitrophenyl phosphate (Sigma) as recommended by the manufacturer, and optical density at 405 nm (OD405 ) was obtained with a microplate reader (Molecular Devices).

    Enzyme-linked Immunosorbent Assay:

    Article Title: A Vaccine and Monoclonal Antibodies That Enhance Mouse Resistance to Candida albicans Vaginal Infection
    Article Snippet: Briefly, one half of the number of wells in an ELISA 96-microtiter plate (Corning Glass, Corning, N.Y.) received 50 μl of the enriched mannan fraction, which was used for preparation of the L-mann vaccine. .. The plate was incubated at 37°C for 3 h followed by 5 to 8°C for 16 h. The wells were washed with deionized water three times, blocked with 3% bovine serum albumin (BSA) in Tris (Sigma)-buffered saline solution (TBS; 0.01 M Tris-HCl, 0.15 M NaCl [pH 7.4]) (TBS-BSA) at room temperature (RT; 22 to 24°C) for 1 h, washed with TBS plus 0.05% Tween 20 (TBS-Tween) three times, and washed with TBS three times.

    Article Title: Protective Immunity against Congenital Toxoplasmosis with Recombinant SAG1 Protein in a Guinea Pig Model
    Article Snippet: Animals were bled 2 weeks after the last immunization, and sera were tested for the presence of anti-SAG1 immunoglobulin G (IgG) antibodies in an enzyme-linked immunosorbent assay (ELISA). .. Plates were then washed five times with Tris-buffered saline (TBS)-Tween buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 0.1% Tween 80) and saturated for 1 h at 37°C with 150 μl of the same buffer supplemented with 1% bovine serum albumin (Sigma).

    Article Title: Identification of Sites in Adenovirus Hexon for Foreign Peptide Incorporation
    Article Snippet: Paragraph title: ELISA. ... After extensive washes with 0.05% Tween 20 in Tris-buffered saline (TBS) and blocking with blocking solution (2% bovine serum albumin and 0.05% Tween 20 in TBS), the immobilized viruses were incubated with anti-His tag monoclonal antibody (Chemicon) for 2 h at room temperature, followed by AP-conjugated goat anti-mouse antibody incubation.

    Sandwich ELISA:

    Article Title: Effects of fermented ginseng on memory impairment and ?-amyloid reduction in Alzheimer's disease experimental models
    Article Snippet: The brain samples (100 mg) were homogenized in Tris-buffered saline solution (20 mM Tris, 137 mM NaCl, pH 7.4) containing a complete protease inhibitors tablet (catalog no. P8340; Sigma, St. Louis, MO, USA). .. Soluble Aβ42 levels in the resulting supernatants were determined using the same sandwich ELISA kit used for the in vitro detection.

    Incubation:

    Article Title: The non-inflammatory role of C1q during Her2/neu-driven mammary carcinogenesis
    Article Snippet: .. Membranes were blocked in 5% BSA (Sigma Aldrich) Tris-buffered saline (TBS)-Tween buffer (137 mM NaCl, 20 mM Tris/HCl, pH 7.6, 0.05% Tween-20) for 1 h at RT and then incubated with appropriate primary (anti e-Cadherin, Cell Signaling, 3195; anti β-actin, Santa Cruz Biotechnology, sc-69879) and appropriate secondary antibodies (goat anti-rabbit, Sigma_Aldrich, A0545 and goat anti mouse Sigma_Aldrich, A4416, respectively) in 3% BSA TBS-Tween buffer overnight at 4°C and for 1 h at room temperature, respectively, and visualized using enhanced chemiluminescence (ECL Plus, Thermo Scientific Pierce). .. Protein modulations were normalized on the actin loading control and expressed as Adjusted Volume Intensity/mm2 (background subtraction) using Quantity ONE software (Biorad, Milano, Italy).

    Article Title: Expression of multidrug resistance protein P-glycoprotein in correlation with markers of hypoxia (HIF-1α, EPO, EPO-R) in invasive breast cancer with metastasis to lymph nodes
    Article Snippet: When antigen epitopes were revealed, sections were cooled for 20 min. After washing twice with distilled water and incubating in 3% hydrogen peroxide for 5 min, the sections were washed with TBS buffer (Tris-Buffered Saline, Code: S1968, pH 7.6) (Sigma). .. The primary antibody was applied in an appropriate dilution and incubated in a moisture chamber for 60 minutes at room temperature.

    Article Title: A Vaccine and Monoclonal Antibodies That Enhance Mouse Resistance to Candida albicans Vaginal Infection
    Article Snippet: .. The plate was incubated at 37°C for 3 h followed by 5 to 8°C for 16 h. The wells were washed with deionized water three times, blocked with 3% bovine serum albumin (BSA) in Tris (Sigma)-buffered saline solution (TBS; 0.01 M Tris-HCl, 0.15 M NaCl [pH 7.4]) (TBS-BSA) at room temperature (RT; 22 to 24°C) for 1 h, washed with TBS plus 0.05% Tween 20 (TBS-Tween) three times, and washed with TBS three times. .. Fifty microliters of vaginal lavage obtained as indicated above was added to the appropriate wells, which were then incubated at 37°C for 1 h and washed with TBS-Tween and TBS as described above.

    Article Title: ERBB3 is required for tumor promotion in a mouse model of skin carcinogenesis), ERBB3 is required for tumor promotion in a mouse model of skin carcinogenesis
    Article Snippet: .. After washing in Tris‐buffered saline solution with 0.1% Tween 20 (Sigma, Taufkirchen, Germany), membranes were incubated over night at 4 °C in 5% w/v BSA (Sigma) with the appropriate primary antibody. .. Membranes were washed and incubated in 5% w/v fat‐free milk powder with a horseradish peroxidase‐labeled secondary antibody donkey anti‐rabbit (1:2000; NA934V, GE Healthcare, Munich, Germany).

    Article Title: Naringenin Ameliorates Drosophila ReepA Hereditary Spastic Paraplegia-Linked Phenotypes
    Article Snippet: .. Membranes were blocked in 10% non-fat dried milk in Tris-Buffer Saline and 0.1% Tween 20 (Sigma-Aldrich, MO, United States) (TBST) as in and were incubated using primary antibody rabbit anti-phospho eIF2aS1 (1:1000, ab32157, Abcam, Cambridge, United Kingdom) overnight at 4°C. .. Mouse anti-α-tubulin (1:2000, T9026, Sigma-Aldrich, St. Louis, MO, United States) was used as loading control.

    Article Title: Protein tyrosine kinase 6 regulates mammary gland tumorigenesis in mouse models
    Article Snippet: .. Polyvinylidene difluoride membranes were blocked in Tris-Buffered Saline Tween-20 solution (150 mM NaCl, 20 mM pH 7.5 Tris and 1% Tween-20) with 5% non-fat milk or bovine serum albumin (Sigma-Aldrich) for 1 h at room temperature, then incubated in primary antibody for 1 h at room temperature or overnight at 4 °C according to the antibody manufacturers' recommendations. .. Antibodies Anti-human PTK6 (C-18) and anti-mouse PTK6 (C-17) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Article Title: Identification of Sites in Adenovirus Hexon for Foreign Peptide Incorporation
    Article Snippet: .. After extensive washes with 0.05% Tween 20 in Tris-buffered saline (TBS) and blocking with blocking solution (2% bovine serum albumin and 0.05% Tween 20 in TBS), the immobilized viruses were incubated with anti-His tag monoclonal antibody (Chemicon) for 2 h at room temperature, followed by AP-conjugated goat anti-mouse antibody incubation. .. Color reaction was performed with p -nitrophenyl phosphate (Sigma) as recommended by the manufacturer, and optical density at 405 nm (OD405 ) was obtained with a microplate reader (Molecular Devices).

    Activity Assay:

    Article Title: Protective Immunity against Congenital Toxoplasmosis with Recombinant SAG1 Protein in a Guinea Pig Model
    Article Snippet: Plates were then washed five times with Tris-buffered saline (TBS)-Tween buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 0.1% Tween 80) and saturated for 1 h at 37°C with 150 μl of the same buffer supplemented with 1% bovine serum albumin (Sigma). .. Enzymatic activity was measured using the p -nitrophenylphosphate substrate (Sigma) dissolved in diethanolamine buffer (pH 9.8).

    Expressing:

    Article Title: Domains in the SPT5 Protein That Modulate Its Transcriptional Regulatory Properties
    Article Snippet: Expression vectors containing Flag-tagged SPT5 cDNA either alone or in combination with expression vectors containing Myc-tagged SPT4 cDNA were transfected into COS cells using Fugene 6 (Roche). .. Flag-tagged fusion proteins were eluted with a Tris-buffered saline solution containing Flag peptide (Sigma) at 100 μg/ml.

    BIA-KA:

    Article Title: The non-inflammatory role of C1q during Her2/neu-driven mammary carcinogenesis
    Article Snippet: Samples were then centrifuged twice at 12,000 rpm at 4°C for 5 min. 40 μg of total proteins, as determined by BCA Protein Assay (Pierce, Thermo Fisher), were separated by SDS-PAGE and electroblotted onto polyvinylidene fluoride membranes (BioRad). .. Membranes were blocked in 5% BSA (Sigma Aldrich) Tris-buffered saline (TBS)-Tween buffer (137 mM NaCl, 20 mM Tris/HCl, pH 7.6, 0.05% Tween-20) for 1 h at RT and then incubated with appropriate primary (anti e-Cadherin, Cell Signaling, 3195; anti β-actin, Santa Cruz Biotechnology, sc-69879) and appropriate secondary antibodies (goat anti-rabbit, Sigma_Aldrich, A0545 and goat anti mouse Sigma_Aldrich, A4416, respectively) in 3% BSA TBS-Tween buffer overnight at 4°C and for 1 h at room temperature, respectively, and visualized using enhanced chemiluminescence (ECL Plus, Thermo Scientific Pierce).

    Western Blot:

    Article Title: ERBB3 is required for tumor promotion in a mouse model of skin carcinogenesis), ERBB3 is required for tumor promotion in a mouse model of skin carcinogenesis
    Article Snippet: Paragraph title: 2.6. Western blots ... After washing in Tris‐buffered saline solution with 0.1% Tween 20 (Sigma, Taufkirchen, Germany), membranes were incubated over night at 4 °C in 5% w/v BSA (Sigma) with the appropriate primary antibody.

    Article Title: Naringenin Ameliorates Drosophila ReepA Hereditary Spastic Paraplegia-Linked Phenotypes
    Article Snippet: Paragraph title: Western Blotting ... Membranes were blocked in 10% non-fat dried milk in Tris-Buffer Saline and 0.1% Tween 20 (Sigma-Aldrich, MO, United States) (TBST) as in and were incubated using primary antibody rabbit anti-phospho eIF2aS1 (1:1000, ab32157, Abcam, Cambridge, United Kingdom) overnight at 4°C.

    Flow Cytometry:

    Article Title: First steps in the standardization of immunoglobulin IgG myeloperoxidase‐anti‐neutrophil cytoplasmic antibody measurements
    Article Snippet: .. SSIB plasma diluted with Tris‐buffered saline (TBS) solution pH 8 (Sigma‐Aldrich) in a 1 : 1 (v/v) ratio was passed over the affinity column at a flow rate of 0·5 ml/min in order to allow antigen–antibody complex formation. .. After washing the column the bound antibodies were eluted by a brief exposure to a 100 mM glycine solution pH 2·7 (Sigma‐Aldrich).

    Immunohistochemistry:

    Article Title: Expression of multidrug resistance protein P-glycoprotein in correlation with markers of hypoxia (HIF-1α, EPO, EPO-R) in invasive breast cancer with metastasis to lymph nodes
    Article Snippet: Paragraph title: Immunohistochemical staining for steroid receptor and HER2 (ER, PR and HER2) ... When antigen epitopes were revealed, sections were cooled for 20 min. After washing twice with distilled water and incubating in 3% hydrogen peroxide for 5 min, the sections were washed with TBS buffer (Tris-Buffered Saline, Code: S1968, pH 7.6) (Sigma).

    Chromatography:

    Article Title: First steps in the standardization of immunoglobulin IgG myeloperoxidase‐anti‐neutrophil cytoplasmic antibody measurements
    Article Snippet: Two chromatographic techniques were combined: immunoaffinity chromatography and size exclusion chromatography (SEC). .. SSIB plasma diluted with Tris‐buffered saline (TBS) solution pH 8 (Sigma‐Aldrich) in a 1 : 1 (v/v) ratio was passed over the affinity column at a flow rate of 0·5 ml/min in order to allow antigen–antibody complex formation.

    other:

    Article Title: In vitro evaluation of local antibiotic delivery via fibrin hydrogel
    Article Snippet: Standard solution preparation 1000 μg/ml ciprofloxacin hydrochloride USP standard stock solution was prepared by dissolving 10 mg ciprofloxacin hydrochloride (Sigma–Aldrich, St. Louis, Missouri, USA) in 10 ml of tris-buffered saline solution (TBS) which consisted of TrisHCl 100 mM (Sigma–Aldrich) and NaCl 150 mM (Sigma–Aldrich) adjusted pH to 7.6 by using NaOH and HCl.

    Affinity Purification:

    Article Title: Domains in the SPT5 Protein That Modulate Its Transcriptional Regulatory Properties
    Article Snippet: Paragraph title: Affinity purification of Flag-tagged SPT5 proteins from COS cells. ... Flag-tagged fusion proteins were eluted with a Tris-buffered saline solution containing Flag peptide (Sigma) at 100 μg/ml.

    Recombinant:

    Article Title: Protective Immunity against Congenital Toxoplasmosis with Recombinant SAG1 Protein in a Guinea Pig Model
    Article Snippet: Briefly, immunoplates were coated with recombinant SAG1 (100 ng/well) for 16 h at 4°C. .. Plates were then washed five times with Tris-buffered saline (TBS)-Tween buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 0.1% Tween 80) and saturated for 1 h at 37°C with 150 μl of the same buffer supplemented with 1% bovine serum albumin (Sigma).

    In Vivo:

    Article Title: Effects of fermented ginseng on memory impairment and ?-amyloid reduction in Alzheimer's disease experimental models
    Article Snippet: Paragraph title: In vivo soluble β-amyloid 42 detection ... The brain samples (100 mg) were homogenized in Tris-buffered saline solution (20 mM Tris, 137 mM NaCl, pH 7.4) containing a complete protease inhibitors tablet (catalog no. P8340; Sigma, St. Louis, MO, USA).

    Isolation:

    Article Title: A Vaccine and Monoclonal Antibodies That Enhance Mouse Resistance to Candida albicans Vaginal Infection
    Article Snippet: An ELISA, as described previously for the isolation of MAb B6.1 , was used to test vaginal washes for the presence of antibody. .. The plate was incubated at 37°C for 3 h followed by 5 to 8°C for 16 h. The wells were washed with deionized water three times, blocked with 3% bovine serum albumin (BSA) in Tris (Sigma)-buffered saline solution (TBS; 0.01 M Tris-HCl, 0.15 M NaCl [pH 7.4]) (TBS-BSA) at room temperature (RT; 22 to 24°C) for 1 h, washed with TBS plus 0.05% Tween 20 (TBS-Tween) three times, and washed with TBS three times.

    Transfection:

    Article Title: Domains in the SPT5 Protein That Modulate Its Transcriptional Regulatory Properties
    Article Snippet: Expression vectors containing Flag-tagged SPT5 cDNA either alone or in combination with expression vectors containing Myc-tagged SPT4 cDNA were transfected into COS cells using Fugene 6 (Roche). .. Flag-tagged fusion proteins were eluted with a Tris-buffered saline solution containing Flag peptide (Sigma) at 100 μg/ml.

    Purification:

    Article Title: First steps in the standardization of immunoglobulin IgG myeloperoxidase‐anti‐neutrophil cytoplasmic antibody measurements
    Article Snippet: The column used for immunoaffinity was prepared in‐house using commercially available purified human myeloperoxidase (Scipac, Sittingbourne, UK) coupled covalently to CNBr activated Sepharose 4B (GE Healthcare, Uppsala, Sweden), according to the protocol provided by the manufacturer. .. SSIB plasma diluted with Tris‐buffered saline (TBS) solution pH 8 (Sigma‐Aldrich) in a 1 : 1 (v/v) ratio was passed over the affinity column at a flow rate of 0·5 ml/min in order to allow antigen–antibody complex formation.

    Article Title: Naringenin Ameliorates Drosophila ReepA Hereditary Spastic Paraplegia-Linked Phenotypes
    Article Snippet: Western Blotting For western blots, total proteins were obtained from 10 adult flies using GRS FullSample purification kit (Grisp, Research solutions, Porto, Portugal). .. Membranes were blocked in 10% non-fat dried milk in Tris-Buffer Saline and 0.1% Tween 20 (Sigma-Aldrich, MO, United States) (TBST) as in and were incubated using primary antibody rabbit anti-phospho eIF2aS1 (1:1000, ab32157, Abcam, Cambridge, United Kingdom) overnight at 4°C.

    Affinity Column:

    Article Title: First steps in the standardization of immunoglobulin IgG myeloperoxidase‐anti‐neutrophil cytoplasmic antibody measurements
    Article Snippet: .. SSIB plasma diluted with Tris‐buffered saline (TBS) solution pH 8 (Sigma‐Aldrich) in a 1 : 1 (v/v) ratio was passed over the affinity column at a flow rate of 0·5 ml/min in order to allow antigen–antibody complex formation. .. After washing the column the bound antibodies were eluted by a brief exposure to a 100 mM glycine solution pH 2·7 (Sigma‐Aldrich).

    Mouse Assay:

    Article Title: Effects of fermented ginseng on memory impairment and ?-amyloid reduction in Alzheimer's disease experimental models
    Article Snippet: In vivo soluble β-amyloid 42 detection For in vivo detection of brain soluble Aβ42, the TG mice that had undergone behavioral tests were anesthetized and decapitated, and the cerebral cortex was dissected from the exposed brains. .. The brain samples (100 mg) were homogenized in Tris-buffered saline solution (20 mM Tris, 137 mM NaCl, pH 7.4) containing a complete protease inhibitors tablet (catalog no. P8340; Sigma, St. Louis, MO, USA).

    SDS Page:

    Article Title: The non-inflammatory role of C1q during Her2/neu-driven mammary carcinogenesis
    Article Snippet: Samples were then centrifuged twice at 12,000 rpm at 4°C for 5 min. 40 μg of total proteins, as determined by BCA Protein Assay (Pierce, Thermo Fisher), were separated by SDS-PAGE and electroblotted onto polyvinylidene fluoride membranes (BioRad). .. Membranes were blocked in 5% BSA (Sigma Aldrich) Tris-buffered saline (TBS)-Tween buffer (137 mM NaCl, 20 mM Tris/HCl, pH 7.6, 0.05% Tween-20) for 1 h at RT and then incubated with appropriate primary (anti e-Cadherin, Cell Signaling, 3195; anti β-actin, Santa Cruz Biotechnology, sc-69879) and appropriate secondary antibodies (goat anti-rabbit, Sigma_Aldrich, A0545 and goat anti mouse Sigma_Aldrich, A4416, respectively) in 3% BSA TBS-Tween buffer overnight at 4°C and for 1 h at room temperature, respectively, and visualized using enhanced chemiluminescence (ECL Plus, Thermo Scientific Pierce).

    Article Title: ERBB3 is required for tumor promotion in a mouse model of skin carcinogenesis), ERBB3 is required for tumor promotion in a mouse model of skin carcinogenesis
    Article Snippet: 30 μg of total protein were separated by 8% or 12% SDS‐PAGE and transferred to PVDF membranes (Millipore, Schwalbach, Germany) by semidry blotting. .. After washing in Tris‐buffered saline solution with 0.1% Tween 20 (Sigma, Taufkirchen, Germany), membranes were incubated over night at 4 °C in 5% w/v BSA (Sigma) with the appropriate primary antibody.

    Software:

    Article Title: The non-inflammatory role of C1q during Her2/neu-driven mammary carcinogenesis
    Article Snippet: Membranes were blocked in 5% BSA (Sigma Aldrich) Tris-buffered saline (TBS)-Tween buffer (137 mM NaCl, 20 mM Tris/HCl, pH 7.6, 0.05% Tween-20) for 1 h at RT and then incubated with appropriate primary (anti e-Cadherin, Cell Signaling, 3195; anti β-actin, Santa Cruz Biotechnology, sc-69879) and appropriate secondary antibodies (goat anti-rabbit, Sigma_Aldrich, A0545 and goat anti mouse Sigma_Aldrich, A4416, respectively) in 3% BSA TBS-Tween buffer overnight at 4°C and for 1 h at room temperature, respectively, and visualized using enhanced chemiluminescence (ECL Plus, Thermo Scientific Pierce). .. Protein modulations were normalized on the actin loading control and expressed as Adjusted Volume Intensity/mm2 (background subtraction) using Quantity ONE software (Biorad, Milano, Italy).

    Article Title: Naringenin Ameliorates Drosophila ReepA Hereditary Spastic Paraplegia-Linked Phenotypes
    Article Snippet: Membranes were blocked in 10% non-fat dried milk in Tris-Buffer Saline and 0.1% Tween 20 (Sigma-Aldrich, MO, United States) (TBST) as in and were incubated using primary antibody rabbit anti-phospho eIF2aS1 (1:1000, ab32157, Abcam, Cambridge, United Kingdom) overnight at 4°C. .. The protein bands were detected using the C400 Azure chemiluminescence biosystem (Aurogene, Rome, Italy) and band densities were quantified with ImageJ Fiji 1.52 software.

    Binding Assay:

    Article Title: Identification of Sites in Adenovirus Hexon for Foreign Peptide Incorporation
    Article Snippet: The enzyme-linked immunosorbent assay (ELISA) binding assay was performed essentially as described previously ( ). .. After extensive washes with 0.05% Tween 20 in Tris-buffered saline (TBS) and blocking with blocking solution (2% bovine serum albumin and 0.05% Tween 20 in TBS), the immobilized viruses were incubated with anti-His tag monoclonal antibody (Chemicon) for 2 h at room temperature, followed by AP-conjugated goat anti-mouse antibody incubation.

    In Vitro:

    Article Title: Domains in the SPT5 Protein That Modulate Its Transcriptional Regulatory Properties
    Article Snippet: Flag-tagged fusion proteins were eluted with a Tris-buffered saline solution containing Flag peptide (Sigma) at 100 μg/ml. .. Proteins were dialysed against buffer D and used in the in vitro transcription assays.

    Article Title: Effects of fermented ginseng on memory impairment and ?-amyloid reduction in Alzheimer's disease experimental models
    Article Snippet: The brain samples (100 mg) were homogenized in Tris-buffered saline solution (20 mM Tris, 137 mM NaCl, pH 7.4) containing a complete protease inhibitors tablet (catalog no. P8340; Sigma, St. Louis, MO, USA). .. Soluble Aβ42 levels in the resulting supernatants were determined using the same sandwich ELISA kit used for the in vitro detection.

    Size-exclusion Chromatography:

    Article Title: First steps in the standardization of immunoglobulin IgG myeloperoxidase‐anti‐neutrophil cytoplasmic antibody measurements
    Article Snippet: Two chromatographic techniques were combined: immunoaffinity chromatography and size exclusion chromatography (SEC). .. SSIB plasma diluted with Tris‐buffered saline (TBS) solution pH 8 (Sigma‐Aldrich) in a 1 : 1 (v/v) ratio was passed over the affinity column at a flow rate of 0·5 ml/min in order to allow antigen–antibody complex formation.

    Acid Assay:

    Article Title: ERBB3 is required for tumor promotion in a mouse model of skin carcinogenesis), ERBB3 is required for tumor promotion in a mouse model of skin carcinogenesis
    Article Snippet: Protein was extracted using Laemmli‐extraction‐buffer, and the concentration was estimated via bicinchoninic acid assay. .. After washing in Tris‐buffered saline solution with 0.1% Tween 20 (Sigma, Taufkirchen, Germany), membranes were incubated over night at 4 °C in 5% w/v BSA (Sigma) with the appropriate primary antibody.

    Concentration Assay:

    Article Title: ERBB3 is required for tumor promotion in a mouse model of skin carcinogenesis), ERBB3 is required for tumor promotion in a mouse model of skin carcinogenesis
    Article Snippet: Protein was extracted using Laemmli‐extraction‐buffer, and the concentration was estimated via bicinchoninic acid assay. .. After washing in Tris‐buffered saline solution with 0.1% Tween 20 (Sigma, Taufkirchen, Germany), membranes were incubated over night at 4 °C in 5% w/v BSA (Sigma) with the appropriate primary antibody.

    Staining:

    Article Title: Expression of multidrug resistance protein P-glycoprotein in correlation with markers of hypoxia (HIF-1α, EPO, EPO-R) in invasive breast cancer with metastasis to lymph nodes
    Article Snippet: Paragraph title: Immunohistochemical staining for steroid receptor and HER2 (ER, PR and HER2) ... When antigen epitopes were revealed, sections were cooled for 20 min. After washing twice with distilled water and incubating in 3% hydrogen peroxide for 5 min, the sections were washed with TBS buffer (Tris-Buffered Saline, Code: S1968, pH 7.6) (Sigma).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore tris buffered saline replaced millipore assay buffer
    Tris Buffered Saline Replaced Millipore Assay Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris buffered saline replaced millipore assay buffer/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    tris buffered saline replaced millipore assay buffer - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results