triple quad tm 5500 mass spectrometer  (SCIEX)

Journal: Journal of proteome research

Article Title: Rapid Verification of Candidate Serological Biomarkers Using Gel-based, Label-free Multiple Reaction Monitoring

doi: 10.1021/pr2002098

Figure Lengend Snippet: GeLC-MRM quantitation of two, heavy, isotope-labeled CTSD peptides using a 5500 QTRAP mass spectrometer. Heavy peptides were spiked at 3, 8, 20, 51, 128, 320, 800, and 4000 amol into a tryptic digest of a fraction of depleted human serum after 1-D gel

Article Snippet: MRM experiments were performed on a 4000 QTRAP® or a 5500 QTRAP hybrid triple quadrupole/linear ion trap mass spectrometer (AB Sciex, Foster City, CA) interfaced with a nanoACQUITY UPLC® system.

Techniques: Quantitation Assay, Labeling, Mass Spectrometry

Journal: Scientific Reports

Article Title: Serial-omics of P53−/−, Brca1−/− Mouse Breast Tumor and Normal Mammary Gland

doi: 10.1038/s41598-017-15132-y

Figure Lengend Snippet: Workflow of the Serial-omics experiment. 10 mg of breast tumor tissue and 10 mg of normal mammary gland were harvested from the mouse and snap frozen separately in liquid nitrogen. The frozen tissue was ground to a powder over dry ice. The powder was solubilized in PBS and extracted with methyltert-butyl ether (MTBE), methanol and water. The upper non-polar liquid phase was collected, dried out and analyzed for untargeted lipidomics via DDA with a Thermo QExactive Plus high resolution Orbitrap mass spectrometer. The lower liquid phase was collected, dried out and analyzed with polarity switching for polar metabolomics via both targeted metabolomics (AB/SCIEX 5500 QTRAP hybrid triple quadrupole mass spectrometer) and untargeted metabolomics (high resolution Thermo QExactive HF Orbitrap). The precipitated protein pellet was re-suspended in sample buffer and separated via a SDS-PAGE gel, fractionated, digested with trypsin and enriched for phosphopeptides via TiO 2 . The digestion mixture and TiO 2 enriched phosphopeptides were analyzed by DDA in pos mode with a Thermo QExactive HF.

Article Snippet: Metabolomics Half of the metabolite samples were re-suspended in 20 μL LC/MS grade water, 5 μL were injected over a 15 min gradient using a hybrid 5500 QTRAP triple quadrupole mass spectrometer (AB/SCIEX) coupled to a Prominence UFLC HPLC system (Shimadzu) via SRM of a total of 287 SRM transitions using positive/negative polarity switching corresponding to 258 unique endogenous water soluble metabolites.

Techniques: Mass Spectrometry, SDS Page

Journal: Journal of colloid and interface science

Article Title: Tannic acid-inspired paclitaxel nanoparticles for enhanced anticancer effects in breast cancer cells

doi: 10.1016/j.jcis.2018.09.072

Figure Lengend Snippet: Intracellular uptake of TAP NPs in breast cancer MDA-MB-231 cells. A) Cellular uptake of Coumarin 6 uptake in breast cancer MDA-MB-231 cells were confirmed by fluorescence microscopy an EVOS® 214 FL Imaging System, in a time dependent manner with dye loaded nanoparticles. B) Intracellular uptake of TAP NPs by LC-MS/MS in MDA-MB-231 after treatment with PTX and TAP NPs for 2, 4 and 6 hours. Cells were treated with PTX and TAP NPs (equivalent to 500 ng of PTX) resulting in 95.52% of drug internalization by TAP NPs in 6 hours in contrast to only 57.19% by native drug PTX for the same time. Data presented as mean ± standard error of the mean (n = 3). A Liquid chromatography–tandem mass spectrometry (LC-MS/MS) (Shimadzu Corporation, Kyoto, Japan) connected to Triple Quad 5500 tandem mass spectrometer (AB SCIEX, Framingham, MA, at a flow rate of 0.8 mL/min was used to determine drug concentration(s) in cells.

Article Snippet: The concentrations of PTX in the TAP NPs were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using an LC system (Shimadzu Corporation, Kyoto, Japan) connected to a Triple Quad 5500 tandem mass spectrometer (AB SCIEX, Framingham, MA).

Techniques: Multiple Displacement Amplification, Fluorescence, Microscopy, Imaging, Liquid Chromatography with Mass Spectroscopy, Liquid Chromatography, Mass Spectrometry, Concentration Assay