Journal: Journal of colloid and interface science
Article Title: Tannic acid-inspired paclitaxel nanoparticles for enhanced anticancer effects in breast cancer cells
Figure Lengend Snippet: Intracellular uptake of TAP NPs in breast cancer MDA-MB-231 cells. A) Cellular uptake of Coumarin 6 uptake in breast cancer MDA-MB-231 cells were confirmed by fluorescence microscopy an EVOS® 214 FL Imaging System, in a time dependent manner with dye loaded nanoparticles. B) Intracellular uptake of TAP NPs by LC-MS/MS in MDA-MB-231 after treatment with PTX and TAP NPs for 2, 4 and 6 hours. Cells were treated with PTX and TAP NPs (equivalent to 500 ng of PTX) resulting in 95.52% of drug internalization by TAP NPs in 6 hours in contrast to only 57.19% by native drug PTX for the same time. Data presented as mean ± standard error of the mean (n = 3). A Liquid chromatography–tandem mass spectrometry (LC-MS/MS) (Shimadzu Corporation, Kyoto, Japan) connected to Triple Quad 5500 tandem mass spectrometer (AB SCIEX, Framingham, MA, at a flow rate of 0.8 mL/min was used to determine drug concentration(s) in cells.
Article Snippet: The concentrations of PTX in the TAP NPs were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using an LC system (Shimadzu Corporation, Kyoto, Japan) connected to a Triple Quad 5500 tandem mass spectrometer (AB SCIEX, Framingham, MA).
Techniques: Multiple Displacement Amplification, Fluorescence, Microscopy, Imaging, Liquid Chromatography with Mass Spectroscopy, Liquid Chromatography, Mass Spectrometry, Concentration Assay