triple quad 6500 system mass spectrometer  (SCIEX)

 
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    SCIEX triple quad 6500 system mass spectrometer
    Triple Quad 6500 System Mass Spectrometer, supplied by SCIEX, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triple quad 6500 system mass spectrometer/product/SCIEX
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    triple quad 6500 system mass spectrometer - by Bioz Stars, 2022-05
    99/100 stars

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    SCIEX triple quad 6500 system
    SCIEX Triple <t>Quad</t> 6500 instrument: Spectra recorded in droplet scan mode with thermometer ions sprayed from a water/acetonitrile solution at different collision voltages. Collision gas parameter (CAD) was set to 6. Liquid flow was set to 7 μL/min. The m/z region filtered out in Q1 is indicated by the shaded areas
    Triple Quad 6500 System, supplied by SCIEX, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triple quad 6500 system/product/SCIEX
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    triple quad 6500 system - by Bioz Stars, 2022-05
    86/100 stars
      Buy from Supplier

    99
    SCIEX chromatography tandem triple quadrupole mass spectrometer
    <t>Mass</t> spectral detection and analysis of acyl-ACPs. A) Chromatographic properties of acyl-ACP standards. Asp-N digested partial acyl-ACPs are separated according to the length and properties of the acyl chain on a reversed phase column by liquid <t>chromatography</t> and detected by <t>triple</t> <t>quadrupole</t> mass <t>spectrometer.</t> Labels above peaks represent the acyl species in the acyl-ACP molecules. B) MS/MS fragmentation product ion scan of Asp-N digested 15:0-ACP standard indicating the major losses. C) Optimal mass spectrometry parameters for acyl-ACP detection by multiple reaction monitoring (MRM). Declustering potential (DP), collision energy (CE), collision cell exit potential (CXP) Responses are represented as the slope (ratio of peak areas per pmol analyte) of the calibration curve for each ACP species. Linear regression (R 2 ) weighted by 1/Y for all samples. The standard deviation of the lowest detectable levels and slope of the calibration curve were used to establish the limit of detection (LOD) and quantification (LOQ).
    Chromatography Tandem Triple Quadrupole Mass Spectrometer, supplied by SCIEX, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chromatography tandem triple quadrupole mass spectrometer/product/SCIEX
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chromatography tandem triple quadrupole mass spectrometer - by Bioz Stars, 2022-05
    99/100 stars
      Buy from Supplier

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    SCIEX Triple Quad 6500 instrument: Spectra recorded in droplet scan mode with thermometer ions sprayed from a water/acetonitrile solution at different collision voltages. Collision gas parameter (CAD) was set to 6. Liquid flow was set to 7 μL/min. The m/z region filtered out in Q1 is indicated by the shaded areas

    Journal: Analytical and Bioanalytical Chemistry

    Article Title: Observation of charged droplets from electrospray ionization (ESI) plumes in API mass spectrometers

    doi: 10.1007/s00216-021-03452-y

    Figure Lengend Snippet: SCIEX Triple Quad 6500 instrument: Spectra recorded in droplet scan mode with thermometer ions sprayed from a water/acetonitrile solution at different collision voltages. Collision gas parameter (CAD) was set to 6. Liquid flow was set to 7 μL/min. The m/z region filtered out in Q1 is indicated by the shaded areas

    Article Snippet: In the first set of experiments, the SCIEX Triple Quad 6500 system was run in a “droplet scan mode” by setting Q1 to RF-only mode as described in the “ ” section.

    Techniques:

    SCIEX Triple Quad 6500: Variation of gas pressure (CAD parameter) in the collision cell at a fixed collision energy of 12 V. Measurement of benzylpyridinium thermometer ions sprayed from water/acetonitrile solution in low mass mode. Liquid flow was set to 7 μL/min. The m/z region filtered out in Q1 is indicated by the shaded areas

    Journal: Analytical and Bioanalytical Chemistry

    Article Title: Observation of charged droplets from electrospray ionization (ESI) plumes in API mass spectrometers

    doi: 10.1007/s00216-021-03452-y

    Figure Lengend Snippet: SCIEX Triple Quad 6500: Variation of gas pressure (CAD parameter) in the collision cell at a fixed collision energy of 12 V. Measurement of benzylpyridinium thermometer ions sprayed from water/acetonitrile solution in low mass mode. Liquid flow was set to 7 μL/min. The m/z region filtered out in Q1 is indicated by the shaded areas

    Article Snippet: In the first set of experiments, the SCIEX Triple Quad 6500 system was run in a “droplet scan mode” by setting Q1 to RF-only mode as described in the “ ” section.

    Techniques:

    SCIEX Triple Quad 6500: Droplet scan with reserpine sprayed from isopropanol/water solution at different collision voltages. The m/z region filtered out in Q1 is indicated by the shaded areas

    Journal: Analytical and Bioanalytical Chemistry

    Article Title: Observation of charged droplets from electrospray ionization (ESI) plumes in API mass spectrometers

    doi: 10.1007/s00216-021-03452-y

    Figure Lengend Snippet: SCIEX Triple Quad 6500: Droplet scan with reserpine sprayed from isopropanol/water solution at different collision voltages. The m/z region filtered out in Q1 is indicated by the shaded areas

    Article Snippet: In the first set of experiments, the SCIEX Triple Quad 6500 system was run in a “droplet scan mode” by setting Q1 to RF-only mode as described in the “ ” section.

    Techniques:

    SCIEX Triple Quad 6500: Variation of gas pressure in the collision cell at different collision energies. Measurement of benzylpyridinium thermometer ions sprayed from water/acetonitrile solution in high mass mode. Collision gas parameter (CAD) was set to 6 (left) and 12 (right). Liquid flow was set to 10 μL/min. The m/z region filtered out in Q1 is indicated by the shaded areas

    Journal: Analytical and Bioanalytical Chemistry

    Article Title: Observation of charged droplets from electrospray ionization (ESI) plumes in API mass spectrometers

    doi: 10.1007/s00216-021-03452-y

    Figure Lengend Snippet: SCIEX Triple Quad 6500: Variation of gas pressure in the collision cell at different collision energies. Measurement of benzylpyridinium thermometer ions sprayed from water/acetonitrile solution in high mass mode. Collision gas parameter (CAD) was set to 6 (left) and 12 (right). Liquid flow was set to 10 μL/min. The m/z region filtered out in Q1 is indicated by the shaded areas

    Article Snippet: In the first set of experiments, the SCIEX Triple Quad 6500 system was run in a “droplet scan mode” by setting Q1 to RF-only mode as described in the “ ” section.

    Techniques:

    Mass spectral detection and analysis of acyl-ACPs. A) Chromatographic properties of acyl-ACP standards. Asp-N digested partial acyl-ACPs are separated according to the length and properties of the acyl chain on a reversed phase column by liquid chromatography and detected by triple quadrupole mass spectrometer. Labels above peaks represent the acyl species in the acyl-ACP molecules. B) MS/MS fragmentation product ion scan of Asp-N digested 15:0-ACP standard indicating the major losses. C) Optimal mass spectrometry parameters for acyl-ACP detection by multiple reaction monitoring (MRM). Declustering potential (DP), collision energy (CE), collision cell exit potential (CXP) Responses are represented as the slope (ratio of peak areas per pmol analyte) of the calibration curve for each ACP species. Linear regression (R 2 ) weighted by 1/Y for all samples. The standard deviation of the lowest detectable levels and slope of the calibration curve were used to establish the limit of detection (LOD) and quantification (LOQ).

    Journal: bioRxiv

    Article Title: Quantification and Discovery of Acyl-ACPs by LC-MS/MS

    doi: 10.1101/870485

    Figure Lengend Snippet: Mass spectral detection and analysis of acyl-ACPs. A) Chromatographic properties of acyl-ACP standards. Asp-N digested partial acyl-ACPs are separated according to the length and properties of the acyl chain on a reversed phase column by liquid chromatography and detected by triple quadrupole mass spectrometer. Labels above peaks represent the acyl species in the acyl-ACP molecules. B) MS/MS fragmentation product ion scan of Asp-N digested 15:0-ACP standard indicating the major losses. C) Optimal mass spectrometry parameters for acyl-ACP detection by multiple reaction monitoring (MRM). Declustering potential (DP), collision energy (CE), collision cell exit potential (CXP) Responses are represented as the slope (ratio of peak areas per pmol analyte) of the calibration curve for each ACP species. Linear regression (R 2 ) weighted by 1/Y for all samples. The standard deviation of the lowest detectable levels and slope of the calibration curve were used to establish the limit of detection (LOD) and quantification (LOQ).

    Article Snippet: Liquid chromatography mass spectrometry condition The Asp-N digestion products of acyl-ACPs were analyzed using a liquid chromatography tandem triple quadrupole mass spectrometer (QTRAP® 6500 LC/MS/MS system, SCIEX, Framingham, MA).

    Techniques: Liquid Chromatography, Mass Spectrometry, Tandem Mass Spectroscopy, Standard Deviation

    Acyl-ACP elongation intermediates detected from Camelina developing seeds. Results (means and standard deviations) from a liquid chromatography tandem triple quadrupole mass spectrometer analysis. A) Relative peak areas in percentage were calculated from all the acyl-ACP species detected. The average and standard deviation are presented based on four independent sample preparations. B) Chromatographic detection of unanticipated unsaturated acyl-ACPs. The characteristic double peak pattern for the 2,3- trans -enoyl- and desaturated acyl-ACP isomers were observed for two medium chain acyl-ACPs (C10, dark blue; C14, green). Polyunsaturated C16-ACP (16:3-ACP; black) was also detected.

    Journal: bioRxiv

    Article Title: Quantification and Discovery of Acyl-ACPs by LC-MS/MS

    doi: 10.1101/870485

    Figure Lengend Snippet: Acyl-ACP elongation intermediates detected from Camelina developing seeds. Results (means and standard deviations) from a liquid chromatography tandem triple quadrupole mass spectrometer analysis. A) Relative peak areas in percentage were calculated from all the acyl-ACP species detected. The average and standard deviation are presented based on four independent sample preparations. B) Chromatographic detection of unanticipated unsaturated acyl-ACPs. The characteristic double peak pattern for the 2,3- trans -enoyl- and desaturated acyl-ACP isomers were observed for two medium chain acyl-ACPs (C10, dark blue; C14, green). Polyunsaturated C16-ACP (16:3-ACP; black) was also detected.

    Article Snippet: Liquid chromatography mass spectrometry condition The Asp-N digestion products of acyl-ACPs were analyzed using a liquid chromatography tandem triple quadrupole mass spectrometer (QTRAP® 6500 LC/MS/MS system, SCIEX, Framingham, MA).

    Techniques: Liquid Chromatography, Mass Spectrometry, Standard Deviation

    OxPL detection by LC tripleQ MS with optimized MRM transitions and LC gradient conditions. A: A series of biogenic OxPCs containing HETEs were selectively detected by optimized MRM transitions. B: Structural isomers were separated by the optimized LC gradient condition.

    Journal: Journal of Lipid Research

    Article Title: Comprehensive analyses of oxidized phospholipids using a measured MS/MS spectra library [S]

    doi: 10.1194/jlr.D077123

    Figure Lengend Snippet: OxPL detection by LC tripleQ MS with optimized MRM transitions and LC gradient conditions. A: A series of biogenic OxPCs containing HETEs were selectively detected by optimized MRM transitions. B: Structural isomers were separated by the optimized LC gradient condition.

    Article Snippet: Broad-targeted analysis was performed using an ACQUITY UPLC system coupled with a triple quadrupole (tripleQ) MS (QTRAP 6500; Sciex).

    Techniques: Mass Spectrometry

    Calibration curves for quantification of OxPLs. OxPL standard solutions corresponding to 10, 20, 50, 100, 200, and 500 fmol were quantified by LC tripleQ MS with optimized MRM transitions. Calibration curves were constructed by plotting the peak areas and concentrations.

    Journal: Journal of Lipid Research

    Article Title: Comprehensive analyses of oxidized phospholipids using a measured MS/MS spectra library [S]

    doi: 10.1194/jlr.D077123

    Figure Lengend Snippet: Calibration curves for quantification of OxPLs. OxPL standard solutions corresponding to 10, 20, 50, 100, 200, and 500 fmol were quantified by LC tripleQ MS with optimized MRM transitions. Calibration curves were constructed by plotting the peak areas and concentrations.

    Article Snippet: Broad-targeted analysis was performed using an ACQUITY UPLC system coupled with a triple quadrupole (tripleQ) MS (QTRAP 6500; Sciex).

    Techniques: Mass Spectrometry, Construct