formaldehyde  (Millipore)


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  • 99
    Name:
    Formaldehyde solution
    Description:

    Catalog Number:
    f8923
    Price:
    None
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    Structured Review

    Millipore formaldehyde
    Formaldehyde solution

    https://www.bioz.com/result/formaldehyde/product/Millipore
    Average 99 stars, based on 5836 article reviews
    Price from $9.99 to $1999.99
    formaldehyde - by Bioz Stars, 2020-09
    99/100 stars

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    In Vivo:

    Article Title: Differential and Interactive Effects of Ligand-Bound Progesterone Receptor A and B Isoforms on Tyrosine Hydroxylase Promoter Activity
    Article Snippet: .. Twenty-four hours later, cells were treated with 200 nM progesterone for 0, 1 and 2 h followed by in vivo crosslinking with formaldehyde (Sigma-Aldrich, St. louis, MO). .. ChIP assays were performed using the EZ-ChIP chromatin immunoprecipitation kit (Millipore) following the manufacturer’s protocol with either 2 µg of mouse anti-FLAG antibodies (Sigma-Aldrich) or mouse non-immune IgGs (Santa Cruz Biotechnologies, Santa Cruz, CA).

    Cell Culture:

    Article Title: The pro-inflammatory cytokine TNF-α inhibits lymphatic pumping via activation of the NF-κB - iNOS signaling pathway
    Article Snippet: .. Lymphatic vessels were dissected as described above and similarly incubated in the absence (sham) or presence of TNF-α (10 ng/ml) for 24 h. The vessels were then fixed in PBS containing 4% formalin (Sigma-Aldrich, Oakville, ON, Canada) for 30 min at 37°C, and permeabilized in chilled methanol at −20°C for 5 min. Rat mesenteric lymphatic muscle and endothelial cells were kindly provided by Dr. W. E. Cromer and D. C. Zawieja (TAMUHSC, Temple, TX) and cultured according to their publication [ ]. .. Briefly, both types of cells were grown on 12 mm round coverslips (Warner Instruments, Hamden, CT, USA) pre-coated with 2% porcine gelatin (LECs) in DMEM/F12 supplemented with Antibiotic-Antimycotic (Thermo Fisher Scientific) and FBS (Sigma-Aldrich) for LMCs and EGM-2 (Lonza, Walkersville, MD, USA) for LECs.

    Incubation:

    Article Title: Human Dermal Stem/Progenitor Cell-Derived Conditioned Medium Improves Senescent Human Dermal Fibroblasts
    Article Snippet: .. Immunofluorescence Staining for Ki67 Senescent fibroblasts were incubated with 50% non-hDSPC-CM or hDSPC-CM for 24 h. Following CM treatment, the cells were fixed with 4% formaldehyde (Sigma-Aldrich) and then blocked with 1% bovine serum albumin (Sigma-Aldrich). .. The cells were then incubated with mouse monoclonal anti-Ki67 antibody (Abcam, Cambridge, MA, USA) for 12 h at 4 °C, followed by Alexa-488-conjugated secondary antibody (Invitrogen, Carlsbad, CA, USA) for 1.5 h at room temperature.

    Article Title: The pro-inflammatory cytokine TNF-α inhibits lymphatic pumping via activation of the NF-κB - iNOS signaling pathway
    Article Snippet: .. Lymphatic vessels were dissected as described above and similarly incubated in the absence (sham) or presence of TNF-α (10 ng/ml) for 24 h. The vessels were then fixed in PBS containing 4% formalin (Sigma-Aldrich, Oakville, ON, Canada) for 30 min at 37°C, and permeabilized in chilled methanol at −20°C for 5 min. Rat mesenteric lymphatic muscle and endothelial cells were kindly provided by Dr. W. E. Cromer and D. C. Zawieja (TAMUHSC, Temple, TX) and cultured according to their publication [ ]. .. Briefly, both types of cells were grown on 12 mm round coverslips (Warner Instruments, Hamden, CT, USA) pre-coated with 2% porcine gelatin (LECs) in DMEM/F12 supplemented with Antibiotic-Antimycotic (Thermo Fisher Scientific) and FBS (Sigma-Aldrich) for LMCs and EGM-2 (Lonza, Walkersville, MD, USA) for LECs.

    Activity Assay:

    Article Title: S-Nitrosoglutathione Reductase Is Essential for Protecting the Female Heart from Ischemia-Reperfusion Injury
    Article Snippet: .. Since N6022 had a significant impact on formaldehyde dehydrogenase activity , baseline formaldehyde levels were examined in male and female WT and GSNO-R−/− hearts using a commercially available kit (Sigma-Aldrich). ..

    Staining:

    Article Title: Human Dermal Stem/Progenitor Cell-Derived Conditioned Medium Improves Senescent Human Dermal Fibroblasts
    Article Snippet: .. Immunofluorescence Staining for Ki67 Senescent fibroblasts were incubated with 50% non-hDSPC-CM or hDSPC-CM for 24 h. Following CM treatment, the cells were fixed with 4% formaldehyde (Sigma-Aldrich) and then blocked with 1% bovine serum albumin (Sigma-Aldrich). .. The cells were then incubated with mouse monoclonal anti-Ki67 antibody (Abcam, Cambridge, MA, USA) for 12 h at 4 °C, followed by Alexa-488-conjugated secondary antibody (Invitrogen, Carlsbad, CA, USA) for 1.5 h at room temperature.

    Article Title: Combined treatment of sodium ferulate, n‐butylidenephthalide, and ADSCs rehabilitates neurovascular unit in rats after photothrombotic stroke, et al. Combined treatment of sodium ferulate, n‐butylidenephthalide, and ADSCs rehabilitates neurovascular unit in rats after photothrombotic stroke
    Article Snippet: .. After 2 weeks of induction, cells were fixed with 4% formaldehyde (Sigma‐Aldrich) followed by staining with 0.18% Oil Red O (Sigma‐Aldrich) for 5 minutes or with 1% Alizarin‐red S (Sigma Aldrich) solution in water for 10 minutes. .. The control cells were cultured with maintenance medium and underwent the same staining procedures above.

    Article Title: Enterovirus A71 Infection Activates Human Immune Responses and Induces Pathological Changes in Humanized Mice
    Article Snippet: .. Brain, spinal cord, lung, and limb were harvested and fixed with 10% formalin (Sigma) at room temperature for 72 h. For H & E staining, the fixed tissues were paraffin embedded, sectioned (Leica), and stained with hematoxylin and eosin. .. The IHC staining was performed according to the manufacturers’ instructions with the rabbit-specific IHC polymer detection kit HRP/DAB (Abcam) and mouse-on-mouse polymer IHC kit (Abcam).

    Chromatin Immunoprecipitation:

    Article Title: Epigenetic and Transcriptome Profiling Identifies a Population of Visceral Adipose-Derived Progenitor Cells with the Potential to Differentiate into an Endocrine Pancreatic Lineage
    Article Snippet: .. Briefly, cells were cross-linked using 1% formaldehyde (Sigma, St. Louis, MO, USA) and DNA sheared using a Bioruptor to generate fragments of 200–500 base pairs (bp) required for ChIP. .. Chromatin was immunoprecipitated using 2 µg of specific antibodies for H3K4 trimethyl, H3K9 trimethyl and H3Ac (Merck Millipore, Burlington, MA, USA), with rabbit immunoglobulin (Ig)G (Merck Millipore), used as an isotype control.

    Immunofluorescence:

    Article Title: Human Dermal Stem/Progenitor Cell-Derived Conditioned Medium Improves Senescent Human Dermal Fibroblasts
    Article Snippet: .. Immunofluorescence Staining for Ki67 Senescent fibroblasts were incubated with 50% non-hDSPC-CM or hDSPC-CM for 24 h. Following CM treatment, the cells were fixed with 4% formaldehyde (Sigma-Aldrich) and then blocked with 1% bovine serum albumin (Sigma-Aldrich). .. The cells were then incubated with mouse monoclonal anti-Ki67 antibody (Abcam, Cambridge, MA, USA) for 12 h at 4 °C, followed by Alexa-488-conjugated secondary antibody (Invitrogen, Carlsbad, CA, USA) for 1.5 h at room temperature.

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  • 89
    Millipore ntp mix
    Ntp Mix, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ntp mix/product/Millipore
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ntp mix - by Bioz Stars, 2020-09
    89/100 stars
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