trifluoroacetic acid acn tfa  (Millipore)


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  • 99
    Name:
    Trifluoroacetic acid
    Description:

    Catalog Number:
    t1647
    Price:
    None
    Applications:
    TFA can be used as an ion-pairing reagent for reverse-phase chromatography.
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    Structured Review

    Millipore trifluoroacetic acid acn tfa

    https://www.bioz.com/result/trifluoroacetic acid acn tfa/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trifluoroacetic acid acn tfa - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Smart biomaterials: Surfaces functionalized with proteolytically stable osteoblast-adhesive peptides
    Article Snippet: .. Solvents for chromatography such as acetonitrile and TFA (HPLC grade) were from Sigma-Aldrich. .. Ethanol and methanol were from Avantor Performance Materials (Center Valley, PA, U.S.A.). (3-Aminopropyl) triethoxysilane (APTES 2%) was from Sigma-Aldrich.

    Article Title: MitoNeoD: A Mitochondria-Targeted Superoxide Probe
    Article Snippet: .. Reverse Phase HPLC Analysis Samples were dissolved in 1 or 1.5 mL 25 % Buffer B (0.1% (v/v) trifluoroacetic acid (TFA) in acetonitrile (ACN))/75% Buffer A (0.1% (v/v) TFA in H2 O) and filtered (0.22 μm PVDF filter (Millipore)). .. Samples were then loaded via a 2 mL injection loop onto a C18 RP-HPLC column (Jupiter 300 Å, Phenomenex) with a Widepore C18 guard column.

    Article Title: Highly Potent, Stable, and Selective Dimeric Hetarylpropylguanidine-Type Histamine H2 Receptor Agonists †
    Article Snippet: Preparative HPLC was performed with a system from Knauer (Berlin, Germany) consisting of two K-1800 pumps and a K-2001 detector. .. As mobile phase, 0.1% TFA in millipore water and acetonitrile (MeCN) were used.

    Article Title: Structure‐Activity Relationship of Hetarylpropylguanidines Aiming at the Development of Selective Histamine Receptor Ligands†
    Article Snippet: Preparative HPLC was handled with a system from Knauer (Berlin, Germany) consisting of two K‐1800 pumps and a K‐2001 detector. .. As mobile phase, 0.1 % TFA in millipore water and acetonitrile (MeCN) were used.

    Article Title: Ion-interaction CZE: The presence of high concentrations of ion-pairing reagents demonstrates the complex mechanisms involved in peptide separations
    Article Snippet: .. TFA, PFPA, HFBA, phosphoric acid, lithium hydroxide, and HPLC-grade ACN were purchased from Sigma–Aldrich (St. Louis, MO, USA). ..

    Flow Cytometry:

    Article Title: Highly Potent, Stable, and Selective Dimeric Hetarylpropylguanidine-Type Histamine H2 Receptor Agonists †
    Article Snippet: As mobile phase, 0.1% TFA in millipore water and acetonitrile (MeCN) were used. .. The temperature was 25 °C, the flow rate was 15 mL/min, and UV detection was performed at 220 nm.

    Article Title: Structure‐Activity Relationship of Hetarylpropylguanidines Aiming at the Development of Selective Histamine Receptor Ligands†
    Article Snippet: As mobile phase, 0.1 % TFA in millipore water and acetonitrile (MeCN) were used. .. The temperature was 25 °C, the flow rate 15 mL/min and UV detection was performed at 220 nm.

    Filtration:

    Article Title: Ion-interaction CZE: The presence of high concentrations of ion-pairing reagents demonstrates the complex mechanisms involved in peptide separations
    Article Snippet: TFA, PFPA, HFBA, phosphoric acid, lithium hydroxide, and HPLC-grade ACN were purchased from Sigma–Aldrich (St. Louis, MO, USA). .. Deionized water was purified by an E-pure water filtration device from Barnstead/Thermolyne (Dubuque, IA, USA).

    Chromatography:

    Article Title: Smart biomaterials: Surfaces functionalized with proteolytically stable osteoblast-adhesive peptides
    Article Snippet: .. Solvents for chromatography such as acetonitrile and TFA (HPLC grade) were from Sigma-Aldrich. .. Ethanol and methanol were from Avantor Performance Materials (Center Valley, PA, U.S.A.). (3-Aminopropyl) triethoxysilane (APTES 2%) was from Sigma-Aldrich.

    Article Title: Quantitative Profiling of Feruloylated Arabinoxylan Side-Chains from Graminaceous Cell Walls
    Article Snippet: Chemicals Trifluoroacetic acid (TFA) and sodium borohydride were from Sigma (Seelze, Germany). .. All chromatography solvents (MS grade) were from VWR (Bruchsal, Germany).

    Article Title: Highly Potent, Stable, and Selective Dimeric Hetarylpropylguanidine-Type Histamine H2 Receptor Agonists †
    Article Snippet: High-resolution mass spectrometry (HRMS) was performed on an Agilent 6540 UHD Accurate-Mass quadrupole time-of-flight liquid chromatography/mass spectrometry (MS) system (Agilent Technologies, Santa Clara, CA) using an electrospray ionization (ESI) source. .. As mobile phase, 0.1% TFA in millipore water and acetonitrile (MeCN) were used.

    Fluorescence:

    Article Title: MitoNeoD: A Mitochondria-Targeted Superoxide Probe
    Article Snippet: Reverse Phase HPLC Analysis Samples were dissolved in 1 or 1.5 mL 25 % Buffer B (0.1% (v/v) trifluoroacetic acid (TFA) in acetonitrile (ACN))/75% Buffer A (0.1% (v/v) TFA in H2 O) and filtered (0.22 μm PVDF filter (Millipore)). .. Samples were eluted at 2 mL/min using a Gilson 321 pump to generate the following gradient: 5-55% Buffer B 0-4 min; 55-70% Buffer B 4-16 min; 70-100% Buffer B 16-18 min; 100% Buffer B 18-21 min, 100-5% Buffer B 21-23 min. A220 of column eluent was measured using a Gilson UV/Vis 151 spectrophotometer and fluorescence (550 nm excitation, 590 nm emission for MitoNeoOH: note these wavelengths are different from those used in aqueous buffer) was measured using a Shimadzu RF-10AXL fluorescence detector (λexcitation = 328 nm; λemission = 375 nm) connected in series with the RP-HPLC system described above.

    Synthesized:

    Article Title: The Degradation and Clearance of Poly(N-hydroxypropyl-L-glutamine)-DTPA-Gd as a Blood Pool MRI Contrast Agent
    Article Snippet: .. Poly-N5 -(3-hydroxypropyl)-L-glutamine (PHPG, molecular weight, 5.1 x 105 dalton), succinic anhydride, gadolinium acetate, 1,3-diisopropylcarbodiimide (DIC), 4-dimethylaminopyridine (DMAP), NN , -diisopropylethylamine (DIPEA), trifluoroacetic acid (TFA) and other reagents were obtained from Sigma-Aldrich (St. Louis, MO). p -( S -2-[4-aminobenzyl]-diethylenetriamine penta- tert -butyl acetate) (NH2 -Bz-DTPA[ t -butyl ester]) was synthesized according to previously reported procedures [ ]. ..

    Mass Spectrometry:

    Article Title: Quantitative Profiling of Feruloylated Arabinoxylan Side-Chains from Graminaceous Cell Walls
    Article Snippet: Chemicals Trifluoroacetic acid (TFA) and sodium borohydride were from Sigma (Seelze, Germany). .. All chromatography solvents (MS grade) were from VWR (Bruchsal, Germany).

    Article Title: Highly Potent, Stable, and Selective Dimeric Hetarylpropylguanidine-Type Histamine H2 Receptor Agonists †
    Article Snippet: High-resolution mass spectrometry (HRMS) was performed on an Agilent 6540 UHD Accurate-Mass quadrupole time-of-flight liquid chromatography/mass spectrometry (MS) system (Agilent Technologies, Santa Clara, CA) using an electrospray ionization (ESI) source. .. As mobile phase, 0.1% TFA in millipore water and acetonitrile (MeCN) were used.

    Article Title: Structure‐Activity Relationship of Hetarylpropylguanidines Aiming at the Development of Selective Histamine Receptor Ligands†
    Article Snippet: High resolution mass spectrometry (HRMS) was performed on an Agilent 6540 UHD Accurate‐Mass Q‐TOF LC/MS system (Agilent Technologies, Santa Clara, CA) using an ESI source. .. As mobile phase, 0.1 % TFA in millipore water and acetonitrile (MeCN) were used.

    Isothermal Titration Calorimetry:

    Article Title: Studies on citrullinated LL-37: detection in human airways, antibacterial effects and biophysical properties
    Article Snippet: Paragraph title: Isothermal titration calorimetry ... The 50 µL syringe was filled with 200 µM LL-37 or 200 µM LL-37Cit5 in 0.1% TFA (Sigma-Aldrich) for titration into 164 µL of 62.5 µM E. coli O111:B4 LPS (InVivoGen, San Diego, CA, USA) in 0.1% TFA (Sigma-Aldrich).

    Spectrophotometry:

    Article Title: MitoNeoD: A Mitochondria-Targeted Superoxide Probe
    Article Snippet: Reverse Phase HPLC Analysis Samples were dissolved in 1 or 1.5 mL 25 % Buffer B (0.1% (v/v) trifluoroacetic acid (TFA) in acetonitrile (ACN))/75% Buffer A (0.1% (v/v) TFA in H2 O) and filtered (0.22 μm PVDF filter (Millipore)). .. Samples were eluted at 2 mL/min using a Gilson 321 pump to generate the following gradient: 5-55% Buffer B 0-4 min; 55-70% Buffer B 4-16 min; 70-100% Buffer B 16-18 min; 100% Buffer B 18-21 min, 100-5% Buffer B 21-23 min. A220 of column eluent was measured using a Gilson UV/Vis 151 spectrophotometer and fluorescence (550 nm excitation, 590 nm emission for MitoNeoOH: note these wavelengths are different from those used in aqueous buffer) was measured using a Shimadzu RF-10AXL fluorescence detector (λexcitation = 328 nm; λemission = 375 nm) connected in series with the RP-HPLC system described above.

    Titration:

    Article Title: Studies on citrullinated LL-37: detection in human airways, antibacterial effects and biophysical properties
    Article Snippet: .. The 50 µL syringe was filled with 200 µM LL-37 or 200 µM LL-37Cit5 in 0.1% TFA (Sigma-Aldrich) for titration into 164 µL of 62.5 µM E. coli O111:B4 LPS (InVivoGen, San Diego, CA, USA) in 0.1% TFA (Sigma-Aldrich). .. Data were analyzed with the NanoAnalyze software (TA Instruments-Waters LLC, New Castle, USA).

    Incubation:

    Article Title: Targeted Single-cell microchemical analysis: MS-based peptidomics of individual paraformaldehyde-fixed and immunolabeled neurons
    Article Snippet: The samples were incubated for a few seconds, air-dried, and covered with purified water. .. For direct ir sample profiling, solutions of saturated CHCA dissolved in 60% methanol/water, 0.01% TFA, and 4mg/ml 2,4-Dinitrophenylhydrazine (2,4-DNPH; Sigma-Aldrich, St. Louis, MO) dissolved in 70% acetonitrile/water, 0.5% TFA, were prepared.

    Thin Layer Chromatography:

    Article Title: Probing stereoselective inhibition of the acyl binding site of cholesterol esterase with four diastereomers of 2'-N-?-methylbenzylcarbamyl-1, 1'-bi-2-naphthol
    Article Snippet: 70% pure since the observed K m value for this enzyme catalyzed hydrolysis of PNPB is 1.4 times higher than that for the pure enzyme [ ]) and PNPB were obtained from Sigma; TFA and other chemicals were obtained from Aldrich. .. Silica gel used in liquid chromatography (Licorpre Silica 60, 200–400 mesh), medium pressure liquid chromatography column (LiChroprep Si 60) and thin layer chromatography plates (Kieselgel 60 F254) were obtained from Merck.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Structure‐Activity Relationship of Hetarylpropylguanidines Aiming at the Development of Selective Histamine Receptor Ligands†
    Article Snippet: High resolution mass spectrometry (HRMS) was performed on an Agilent 6540 UHD Accurate‐Mass Q‐TOF LC/MS system (Agilent Technologies, Santa Clara, CA) using an ESI source. .. As mobile phase, 0.1 % TFA in millipore water and acetonitrile (MeCN) were used.

    IA:

    Article Title: Ion-interaction CZE: The presence of high concentrations of ion-pairing reagents demonstrates the complex mechanisms involved in peptide separations
    Article Snippet: TFA, PFPA, HFBA, phosphoric acid, lithium hydroxide, and HPLC-grade ACN were purchased from Sigma–Aldrich (St. Louis, MO, USA). .. Deionized water was purified by an E-pure water filtration device from Barnstead/Thermolyne (Dubuque, IA, USA).

    Injection:

    Article Title: MitoNeoD: A Mitochondria-Targeted Superoxide Probe
    Article Snippet: Reverse Phase HPLC Analysis Samples were dissolved in 1 or 1.5 mL 25 % Buffer B (0.1% (v/v) trifluoroacetic acid (TFA) in acetonitrile (ACN))/75% Buffer A (0.1% (v/v) TFA in H2 O) and filtered (0.22 μm PVDF filter (Millipore)). .. Samples were then loaded via a 2 mL injection loop onto a C18 RP-HPLC column (Jupiter 300 Å, Phenomenex) with a Widepore C18 guard column.

    Purification:

    Article Title: Fabrication, Polarization of Electrospun Polyvinylidene Fluoride Electret Fibers and Effect on Capturing Nanoscale Solid Aerosols †
    Article Snippet: .. Acetone, N ,N -dimethylformamide (DMF) and trifluoroacetic acid (TFA) were purchased from Sigma Aldrich (St. Louis, MO, USA) and were used without any further purification. ..

    Article Title: Targeted Single-cell microchemical analysis: MS-based peptidomics of individual paraformaldehyde-fixed and immunolabeled neurons
    Article Snippet: The samples were incubated for a few seconds, air-dried, and covered with purified water. .. For direct ir sample profiling, solutions of saturated CHCA dissolved in 60% methanol/water, 0.01% TFA, and 4mg/ml 2,4-Dinitrophenylhydrazine (2,4-DNPH; Sigma-Aldrich, St. Louis, MO) dissolved in 70% acetonitrile/water, 0.5% TFA, were prepared.

    Article Title: Ion-interaction CZE: The presence of high concentrations of ion-pairing reagents demonstrates the complex mechanisms involved in peptide separations
    Article Snippet: TFA, PFPA, HFBA, phosphoric acid, lithium hydroxide, and HPLC-grade ACN were purchased from Sigma–Aldrich (St. Louis, MO, USA). .. Deionized water was purified by an E-pure water filtration device from Barnstead/Thermolyne (Dubuque, IA, USA).

    Liquid Chromatography:

    Article Title: Probing stereoselective inhibition of the acyl binding site of cholesterol esterase with four diastereomers of 2'-N-?-methylbenzylcarbamyl-1, 1'-bi-2-naphthol
    Article Snippet: 70% pure since the observed K m value for this enzyme catalyzed hydrolysis of PNPB is 1.4 times higher than that for the pure enzyme [ ]) and PNPB were obtained from Sigma; TFA and other chemicals were obtained from Aldrich. .. Silica gel used in liquid chromatography (Licorpre Silica 60, 200–400 mesh), medium pressure liquid chromatography column (LiChroprep Si 60) and thin layer chromatography plates (Kieselgel 60 F254) were obtained from Merck.

    Molecular Weight:

    Article Title: Fabrication, Polarization of Electrospun Polyvinylidene Fluoride Electret Fibers and Effect on Capturing Nanoscale Solid Aerosols †
    Article Snippet: Materials PVDF, trade name Kynar with an average molecular weight of about 250,000, density 1.78 gm/cm3 and melting point between 165 and 172 (as per the MSDS), was supplied by Archema Inc. (King of Prussia, PA, USA). .. Acetone, N ,N -dimethylformamide (DMF) and trifluoroacetic acid (TFA) were purchased from Sigma Aldrich (St. Louis, MO, USA) and were used without any further purification.

    Article Title: The Degradation and Clearance of Poly(N-hydroxypropyl-L-glutamine)-DTPA-Gd as a Blood Pool MRI Contrast Agent
    Article Snippet: .. Poly-N5 -(3-hydroxypropyl)-L-glutamine (PHPG, molecular weight, 5.1 x 105 dalton), succinic anhydride, gadolinium acetate, 1,3-diisopropylcarbodiimide (DIC), 4-dimethylaminopyridine (DMAP), NN , -diisopropylethylamine (DIPEA), trifluoroacetic acid (TFA) and other reagents were obtained from Sigma-Aldrich (St. Louis, MO). p -( S -2-[4-aminobenzyl]-diethylenetriamine penta- tert -butyl acetate) (NH2 -Bz-DTPA[ t -butyl ester]) was synthesized according to previously reported procedures [ ]. ..

    Software:

    Article Title: Studies on citrullinated LL-37: detection in human airways, antibacterial effects and biophysical properties
    Article Snippet: The 50 µL syringe was filled with 200 µM LL-37 or 200 µM LL-37Cit5 in 0.1% TFA (Sigma-Aldrich) for titration into 164 µL of 62.5 µM E. coli O111:B4 LPS (InVivoGen, San Diego, CA, USA) in 0.1% TFA (Sigma-Aldrich). .. Data were analyzed with the NanoAnalyze software (TA Instruments-Waters LLC, New Castle, USA).

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  • 99
    Millipore trifluoroacetic acid acn tfa
    Trifluoroacetic Acid Acn Tfa, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trifluoroacetic acid acn tfa/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trifluoroacetic acid acn tfa - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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