Journal: Nature Communications
Article Title: Locating causal hubs of memory consolidation in spontaneous brain network in male mice
doi: 10.1038/s41467-023-41024-z
Figure Lengend Snippet: a Schematic diagram of target validation using DREADDs inhibition. Representative DREADDs expression (red) in the target areas selected from ( b ) the negative control, ( e ) common networks, and ( g ) network integration. In each subgraph, the left image shows the injection location of AAV-pSyn-hM4D(Gi)-T2A-mScarlet, and the right image shows the fluorescence imaging together with the DAPI staining (blue). c The number of shocks during the learning trials (T1–T5) show a progressive decrease and insignificant change during the probe test (PT) after inhibition of the negative controls, VPM R ( F 5, 20 = 10.80, p < 0.0001; N = 5) and FrA R ( F 5, 20 = 10.65, p < 0.0001; N = 5). d The behavior was comparable to that of the CNO-control (CTL) group ( F 5, 40 = 7.41, p < 0.0001; N = 9). f Similar trends during learning can be seen with DREADDs in the common hubs V1 R ( F 5, 45 = 6.95, p < 0.0001; N = 10), S2 R ( F 5, 35 = 6.02, p = 0.0004; N = 8) and S1BF L ( F 5, 50 = 10.67, p < 0.0001; N = 11), or in ( h ) integrator hubs LAchSh L ( F 5, 30 = 5.31, p = 0.0031; N = 7), LO R (F 5, 35 = 6.13, p = 0.0004; N = 8), VM L ( F 5, 45 = 7.16, p < 0.0001; N = 10) and S1Tr R ( F 5, 30 = 6.40, p = 0.0004; N = 7). After hub inhibition, impaired memory recall was seen, except S1Tr R . i Compared with the CTL (two-sample t-test, one tailed, uncorrected), an increased number of shocks (PT−T5) after inhibition of S1BF L (Cohen’s d = 0.76, p = 0.046), V1 R (Cohen’s d = 0.78, p = 0.045), S2 R (Cohen’s d = 0.94, p = 0.025), LAcbSh L (Cohen’s d = 1.04, p = 0.017) or LO R (Cohen’s d = 1.10, p = 0.0090) was found. Data are represented as mean ± SEM. Unless noted, statistical test is one-way ANOVA with post hoc Dunnett’s multiple comparison test with respect to T5. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. See Supplementary Table for the abbreviations of brain regions. Number of animals is from biological independent mice. Source data are provided as a file. The brain outlines were created with BioRender.com.
Article Snippet: In each subgraph, the left image shows the injection location of AAV-pSyn-hM4D(Gi)-T2A-mScarlet, and the right image shows the fluorescence imaging together with the DAPI staining (blue). c The number of shocks during the learning trials (T1–T5) show a progressive decrease and insignificant change during the probe test (PT) after inhibition of the negative controls, VPM R ( F 5, 20 = 10.80, p < 0.0001; N = 5) and FrA R ( F 5, 20 = 10.65, p < 0.0001; N = 5). d The behavior was comparable to that of the CNO-control (CTL) group ( F 5, 40 = 7.41, p < 0.0001; N = 9). f Similar trends during learning can be seen with DREADDs in the common hubs V1 R ( F 5, 45 = 6.95, p < 0.0001; N = 10), S2 R ( F 5, 35 = 6.02, p = 0.0004; N = 8) and S1BF L ( F 5, 50 = 10.67, p < 0.0001; N = 11), or in ( h ) integrator hubs LAchSh L ( F 5, 30 = 5.31, p = 0.0031; N = 7), LO R (F 5, 35 = 6.13, p = 0.0004; N = 8), VM L ( F 5, 45 = 7.16, p < 0.0001; N = 10) and S1Tr R ( F 5, 30 = 6.40, p = 0.0004; N = 7).
Techniques: Inhibition, Expressing, Negative Control, Injection, Fluorescence, Imaging, Staining, One-tailed Test, Comparison
