tri reagent  (Thermo Fisher)


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    Structured Review

    Thermo Fisher tri reagent
    Tri Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tri reagent/product/Thermo Fisher
    Average 99 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    tri reagent - by Bioz Stars, 2020-04
    99/100 stars

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    Amplification:

    Article Title: Fluoxetine Inhibits Canonical Wnt Signaling to Impair Embryoid Body Morphogenesis: Potential Teratogenic Mechanisms of a Commonly Used Antidepressant
    Article Snippet: Total RNA was extracted from cell suspension prior to aggregation (day 0) and from EBs on days 1–4, using TRI reagent (LifeTechnologies) and Direct-zol RNA MiniPrep kit (Zymo Research, Irvine, California), and processed for cDNA synthesis using M-MLV Reverse Transcriptase (Promega, Madison, Wisconsin) and oligo-dT (18) primer. .. Standard reverse transcription and polymerase chain reaction (RT-PCR) was used to detect mRNAs of Slc6a4 (encoding the SERT) and Slc6a2 (encoding the norepinephrine transporter [NET]), using JumpStart REDTaq DNA Polymerase (Sigma-Aldrich, St Louis, Missouri) with the following conditions: initial denaturation at 94°C for 5 min; 35 cycles of 94°C for 15 s, 60°C for 20 s, and 72°C for 40 s; and a final extension at 72°C for 5 min. Amplified products were resolved in a 1% agarose gel and visualized by staining with ethidium bromide.

    Synthesized:

    Article Title: The A/G Allele of Rs16906252 Predicts for MGMT Methylation and Is Selectively Silenced in Premalignant Lesions from Smokers and in Lung Adenocarcinomas
    Article Snippet: .. Cells were harvested in TRI reagent (Sigma-Aldrich, St. Louis, MO) and mRNA was isolated following TRI reagent instructions. cDNA was synthesized using the high capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA). .. A real time PCR using SYBR® Green as the fluorescent dye was developed to measure the MGMT expression level using the ΔCT method.

    Article Title: Crimean-Congo hemorrhagic fever virus nucleocapsid protein harbors distinct RNA-binding sites in the stalk and head domains
    Article Snippet: Briefly, a single strand DNA sequence harboring a 5′ T7 promoter, followed by either panhandle or 5′ NCR sequence was synthesized by IDT. .. The RNA was labeled with [α-32 P]CTP during synthesis and purified using Tri-Reagent (Ambion) followed by column purification using an miRNAeasy kit (Invitrogen), as previously reported ( ).

    Quantitative RT-PCR:

    Article Title: The Trichoderma harzianum Kelch Protein ThKEL1 Plays a Key Role in Root Colonization and the Induction of Systemic Defense in Brassicaceae Plants
    Article Snippet: The pooled leaves were used for RNA extraction with the TRI reagent (Ambion, Austin, TX, USA), following the manufacturer’s instructions. .. Gene expression was analyzed by reverse transcription PCR (RT-qPCR).

    Real-time Polymerase Chain Reaction:

    Article Title: Teleost IgD+IgM− B Cells Mount Clonally Expanded and Mildly Mutated Intestinal IgD Responses in the Absence of Lymphoid Follicles
    Article Snippet: The intestinal content was removed and the hindgut sampled and placed in TRI-Reagent (Thermo Fisher Scientific) to isolate total RNA following manufacturer's instructions. .. To evaluate the levels of transcription of the membrane and secreted forms of IgM and IgD, a real-time PCR was performed in a LightCycler 96 System instrument (Roche) using FastStart Essential DNA Green Master reagents (Roche) and specific primers previously described ( , ).

    Article Title: Sex‐specific effects of leptin administration to pregnant mice on the placentae and the metabolic phenotypes of offspring
    Article Snippet: .. Relative quantitative real‐time PCR Total RNA was isolated from the individual placenta samples with TRI Reagent (Ambion, Carlsbad, CA, USA) according to the manufacturer’s instructions. ..

    Article Title: The A/G Allele of Rs16906252 Predicts for MGMT Methylation and Is Selectively Silenced in Premalignant Lesions from Smokers and in Lung Adenocarcinomas
    Article Snippet: Paragraph title: Real time PCR for measuring MGMT expression ... Cells were harvested in TRI reagent (Sigma-Aldrich, St. Louis, MO) and mRNA was isolated following TRI reagent instructions. cDNA was synthesized using the high capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA).

    Article Title: EGR2 phosphatase regulates OST1 kinase activity and freezing tolerance in Arabidopsis
    Article Snippet: Total RNA was extracted from 14‐day‐old seedlings with or without cold treatment using TRI Reagent (Thermo Fisher Scientific) and reverse‐transcribed using the M‐MLV Reverse Transcriptase (Promega). .. Quantitative real‐time PCR was performed using Applied Biosystems 7500 Real‐Time PCR system with SYBR Premix Ex Taq (Takara).

    Article Title: Subtle sex differences in vasopressin mRNA expression in the embryonic mouse brain.
    Article Snippet: Arginine vasopressin (AVP) is a neuropeptide which acts centrally to modulate numerous social behaviors. .. Arginine vasopressin (AVP) is a neuropeptide which acts centrally to modulate numerous social behaviors.

    Incubation:

    Article Title: Crimean-Congo hemorrhagic fever virus nucleocapsid protein harbors distinct RNA-binding sites in the stalk and head domains
    Article Snippet: The RNA was labeled with [α-32 P]CTP during synthesis and purified using Tri-Reagent (Ambion) followed by column purification using an miRNAeasy kit (Invitrogen), as previously reported ( ). .. Reaction mixtures were incubated at room temperature for 30–45 min and filtered through nitrocellulose membranes under vacuum.

    Article Title: Beyond secondary structure: primary-sequence determinants license pri-miRNA hairpins for processing
    Article Snippet: To assay cleavage, labeled substrates were incubated with Microprocessor lysate, which was prepared from cells overexpressing Drosha and DGCR8 ( ). .. After extraction using Tri-Reagent (Ambion), substrates and products were resolved on denaturing 5% polyacrylamide gels.

    Article Title: Role of Salicylic Acid and Components of the Phenylpropanoid Pathway in Basal and Cultivar-Related Resistance of Oilseed Rape (Brassica napus) to Verticillium longisporum
    Article Snippet: .. RNA Extraction and Synthesis of cDNA About 100 mg of homogenized fresh hypocotyl tissue was suspended in 1 ml TRI-reagent (Invitrogen™, ThermoFisher) and incubated for 15 min at room temperature. .. Subsequently, 100 µl bromochloropropane was added, and the samples were shaken for 15 s. After incubation for 15 min at room temperature, the mixture was centrifuged for 15 min at 10,159 rpm at 4 °C.

    Expressing:

    Article Title: Sex‐specific effects of leptin administration to pregnant mice on the placentae and the metabolic phenotypes of offspring
    Article Snippet: Relative quantitative real‐time PCR Total RNA was isolated from the individual placenta samples with TRI Reagent (Ambion, Carlsbad, CA, USA) according to the manufacturer’s instructions. .. Applied Biosystems TaqMan gene expression assays (Igf1, Mm0043956_m1; Igf2, Mm00439564_m1; Igf2r, Mm00439576_m1; Slc2a1, Mm00441480_m1; Slc2a3, Mm00441483_m1; Slc38a1, Mm00506391_m1; Slc38a2, Mm00628416_m1; Slc38a4, Mm00459056_m1; ObRb‐LepR, Mm00440181_m1) with β‐actin as an endogenous control [TaqMan endogenous controls with FAM dye label and MGB mouse β‐actin (ACTB)] (Life Technologies Corporation, Carlsbad, CA, USA) and TaqMan Gene Expression Master Mix were used for relative quantitative real‐time PCR (Life Technologies Corporation).

    Article Title: The A/G Allele of Rs16906252 Predicts for MGMT Methylation and Is Selectively Silenced in Premalignant Lesions from Smokers and in Lung Adenocarcinomas
    Article Snippet: Paragraph title: Real time PCR for measuring MGMT expression ... Cells were harvested in TRI reagent (Sigma-Aldrich, St. Louis, MO) and mRNA was isolated following TRI reagent instructions. cDNA was synthesized using the high capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA).

    Article Title: EGR2 phosphatase regulates OST1 kinase activity and freezing tolerance in Arabidopsis
    Article Snippet: Paragraph title: Gene expression assays ... Total RNA was extracted from 14‐day‐old seedlings with or without cold treatment using TRI Reagent (Thermo Fisher Scientific) and reverse‐transcribed using the M‐MLV Reverse Transcriptase (Promega).

    Article Title: The Trichoderma harzianum Kelch Protein ThKEL1 Plays a Key Role in Root Colonization and the Induction of Systemic Defense in Brassicaceae Plants
    Article Snippet: Paragraph title: Gene Expression Studies ... The pooled leaves were used for RNA extraction with the TRI reagent (Ambion, Austin, TX, USA), following the manufacturer’s instructions.

    RNA Binding Assay:

    Article Title: Crimean-Congo hemorrhagic fever virus nucleocapsid protein harbors distinct RNA-binding sites in the stalk and head domains
    Article Snippet: The RNA was labeled with [α-32 P]CTP during synthesis and purified using Tri-Reagent (Ambion) followed by column purification using an miRNAeasy kit (Invitrogen), as previously reported ( ). .. All binding reactions were carried out in RNA-binding buffer (40 m m Tris-HCl, pH 7.4, 80 m m NaCl, 20 m m KCl, 1 m m DTT) at a constant concentration of RNA with increasing concentrations of the protein.

    Polymerase Chain Reaction:

    Article Title: Subtle sex differences in vasopressin mRNA expression in the embryonic mouse brain.
    Article Snippet: Arginine vasopressin (AVP) is a neuropeptide which acts centrally to modulate numerous social behaviors. .. Arginine vasopressin (AVP) is a neuropeptide which acts centrally to modulate numerous social behaviors.

    Article Title: Crimean-Congo hemorrhagic fever virus nucleocapsid protein harbors distinct RNA-binding sites in the stalk and head domains
    Article Snippet: The DNA sequence along with two opposing primers was used as template in a PCR to generate the double strand DNA segment. .. The RNA was labeled with [α-32 P]CTP during synthesis and purified using Tri-Reagent (Ambion) followed by column purification using an miRNAeasy kit (Invitrogen), as previously reported ( ).

    Article Title: The Trichoderma harzianum Kelch Protein ThKEL1 Plays a Key Role in Root Colonization and the Induction of Systemic Defense in Brassicaceae Plants
    Article Snippet: The pooled leaves were used for RNA extraction with the TRI reagent (Ambion, Austin, TX, USA), following the manufacturer’s instructions. .. Gene expression was analyzed by reverse transcription PCR (RT-qPCR).

    Article Title: Fluoxetine Inhibits Canonical Wnt Signaling to Impair Embryoid Body Morphogenesis: Potential Teratogenic Mechanisms of a Commonly Used Antidepressant
    Article Snippet: Paragraph title: Reverse transcription and polymerase chain reaction assays ... Total RNA was extracted from cell suspension prior to aggregation (day 0) and from EBs on days 1–4, using TRI reagent (LifeTechnologies) and Direct-zol RNA MiniPrep kit (Zymo Research, Irvine, California), and processed for cDNA synthesis using M-MLV Reverse Transcriptase (Promega, Madison, Wisconsin) and oligo-dT (18) primer.

    Sequencing:

    Article Title: Crimean-Congo hemorrhagic fever virus nucleocapsid protein harbors distinct RNA-binding sites in the stalk and head domains
    Article Snippet: The DNA sequence along with two opposing primers was used as template in a PCR to generate the double strand DNA segment. .. The RNA was labeled with [α-32 P]CTP during synthesis and purified using Tri-Reagent (Ambion) followed by column purification using an miRNAeasy kit (Invitrogen), as previously reported ( ).

    Sonication:

    Article Title: Subtle sex differences in vasopressin mRNA expression in the embryonic mouse brain.
    Article Snippet: .. Arginine vasopressin (AVP) is a neuropeptide which acts centrally to modulate numerous social behaviors. .. Arginine vasopressin (AVP) is a neuropeptide which acts centrally to modulate numerous social behaviors.

    Injection:

    Article Title: Early genome activation in Drosophila is extensive with an initial tendency for aborted transcripts and retained introns
    Article Snippet: Injected embryos were transferred to a 1.5-mL tube, homogenized in TRI-reagent, and frozen at −80°C. .. RNA was extracted with TRI-reagent (Thermo Fisher Scientific) as directed by the manufacturer's protocol.

    Binding Assay:

    Article Title: Crimean-Congo hemorrhagic fever virus nucleocapsid protein harbors distinct RNA-binding sites in the stalk and head domains
    Article Snippet: The RNA was labeled with [α-32 P]CTP during synthesis and purified using Tri-Reagent (Ambion) followed by column purification using an miRNAeasy kit (Invitrogen), as previously reported ( ). .. All binding reactions were carried out in RNA-binding buffer (40 m m Tris-HCl, pH 7.4, 80 m m NaCl, 20 m m KCl, 1 m m DTT) at a constant concentration of RNA with increasing concentrations of the protein.

    Article Title: Beyond secondary structure: primary-sequence determinants license pri-miRNA hairpins for processing
    Article Snippet: Paragraph title: Binding and cleavage assays ... After extraction using Tri-Reagent (Ambion), substrates and products were resolved on denaturing 5% polyacrylamide gels.

    Isolation:

    Article Title: SCA8 RAN polySer protein preferentially accumulates in white matter regions and is regulated by eIF3F
    Article Snippet: Paragraph title: RNA isolation ... Whole cerebellum was removed, cut into pieces, and lysed in TRI reagent (Invitrogen).

    Article Title: Sex‐specific effects of leptin administration to pregnant mice on the placentae and the metabolic phenotypes of offspring
    Article Snippet: .. Relative quantitative real‐time PCR Total RNA was isolated from the individual placenta samples with TRI Reagent (Ambion, Carlsbad, CA, USA) according to the manufacturer’s instructions. ..

    Article Title: The A/G Allele of Rs16906252 Predicts for MGMT Methylation and Is Selectively Silenced in Premalignant Lesions from Smokers and in Lung Adenocarcinomas
    Article Snippet: .. Cells were harvested in TRI reagent (Sigma-Aldrich, St. Louis, MO) and mRNA was isolated following TRI reagent instructions. cDNA was synthesized using the high capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA). .. A real time PCR using SYBR® Green as the fluorescent dye was developed to measure the MGMT expression level using the ΔCT method.

    Article Title: Subtle sex differences in vasopressin mRNA expression in the embryonic mouse brain.
    Article Snippet: .. Arginine vasopressin (AVP) is a neuropeptide which acts centrally to modulate numerous social behaviors. .. Arginine vasopressin (AVP) is a neuropeptide which acts centrally to modulate numerous social behaviors.

    Article Title: Early genome activation in Drosophila is extensive with an initial tendency for aborted transcripts and retained introns
    Article Snippet: Paragraph title: Isolation of 5-EU-containing RNA and library preparation ... RNA was extracted with TRI-reagent (Thermo Fisher Scientific) as directed by the manufacturer's protocol.

    Multiplex Assay:

    Article Title: Subtle sex differences in vasopressin mRNA expression in the embryonic mouse brain.
    Article Snippet: Arginine vasopressin (AVP) is a neuropeptide which acts centrally to modulate numerous social behaviors. .. Arginine vasopressin (AVP) is a neuropeptide which acts centrally to modulate numerous social behaviors.

    Labeling:

    Article Title: Crimean-Congo hemorrhagic fever virus nucleocapsid protein harbors distinct RNA-binding sites in the stalk and head domains
    Article Snippet: .. The RNA was labeled with [α-32 P]CTP during synthesis and purified using Tri-Reagent (Ambion) followed by column purification using an miRNAeasy kit (Invitrogen), as previously reported ( ). .. Interaction of bacterially expressed and purified CCHFV N protein and its mutants with the RNA of interest was studied by a filter-binding assay, as previously reported ( ).

    Article Title: Beyond secondary structure: primary-sequence determinants license pri-miRNA hairpins for processing
    Article Snippet: To assay cleavage, labeled substrates were incubated with Microprocessor lysate, which was prepared from cells overexpressing Drosha and DGCR8 ( ). .. After extraction using Tri-Reagent (Ambion), substrates and products were resolved on denaturing 5% polyacrylamide gels.

    Purification:

    Article Title: Crimean-Congo hemorrhagic fever virus nucleocapsid protein harbors distinct RNA-binding sites in the stalk and head domains
    Article Snippet: .. The RNA was labeled with [α-32 P]CTP during synthesis and purified using Tri-Reagent (Ambion) followed by column purification using an miRNAeasy kit (Invitrogen), as previously reported ( ). .. Interaction of bacterially expressed and purified CCHFV N protein and its mutants with the RNA of interest was studied by a filter-binding assay, as previously reported ( ).

    Article Title: Early genome activation in Drosophila is extensive with an initial tendency for aborted transcripts and retained introns
    Article Snippet: RNA was extracted with TRI-reagent (Thermo Fisher Scientific) as directed by the manufacturer's protocol. .. Approximately 3.0 µg of total RNA was poly(A)-selected using a Dynabeads mRNA purification kit (Thermo Fisher Scientific), or alternatively, for one sample at each time point, rRNA was depleted from 1.0 µg of total RNA using the Ribo-Zero Gold kit (Illumina).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Fluoxetine Inhibits Canonical Wnt Signaling to Impair Embryoid Body Morphogenesis: Potential Teratogenic Mechanisms of a Commonly Used Antidepressant
    Article Snippet: Total RNA was extracted from cell suspension prior to aggregation (day 0) and from EBs on days 1–4, using TRI reagent (LifeTechnologies) and Direct-zol RNA MiniPrep kit (Zymo Research, Irvine, California), and processed for cDNA synthesis using M-MLV Reverse Transcriptase (Promega, Madison, Wisconsin) and oligo-dT (18) primer. .. Standard reverse transcription and polymerase chain reaction (RT-PCR) was used to detect mRNAs of Slc6a4 (encoding the SERT) and Slc6a2 (encoding the norepinephrine transporter [NET]), using JumpStart REDTaq DNA Polymerase (Sigma-Aldrich, St Louis, Missouri) with the following conditions: initial denaturation at 94°C for 5 min; 35 cycles of 94°C for 15 s, 60°C for 20 s, and 72°C for 40 s; and a final extension at 72°C for 5 min. Amplified products were resolved in a 1% agarose gel and visualized by staining with ethidium bromide.

    Filter-binding Assay:

    Article Title: Crimean-Congo hemorrhagic fever virus nucleocapsid protein harbors distinct RNA-binding sites in the stalk and head domains
    Article Snippet: The RNA was labeled with [α-32 P]CTP during synthesis and purified using Tri-Reagent (Ambion) followed by column purification using an miRNAeasy kit (Invitrogen), as previously reported ( ). .. Interaction of bacterially expressed and purified CCHFV N protein and its mutants with the RNA of interest was studied by a filter-binding assay, as previously reported ( ).

    SYBR Green Assay:

    Article Title: The A/G Allele of Rs16906252 Predicts for MGMT Methylation and Is Selectively Silenced in Premalignant Lesions from Smokers and in Lung Adenocarcinomas
    Article Snippet: Cells were harvested in TRI reagent (Sigma-Aldrich, St. Louis, MO) and mRNA was isolated following TRI reagent instructions. cDNA was synthesized using the high capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA). .. A real time PCR using SYBR® Green as the fluorescent dye was developed to measure the MGMT expression level using the ΔCT method.

    RNA Extraction:

    Article Title: The Trichoderma harzianum Kelch Protein ThKEL1 Plays a Key Role in Root Colonization and the Induction of Systemic Defense in Brassicaceae Plants
    Article Snippet: .. The pooled leaves were used for RNA extraction with the TRI reagent (Ambion, Austin, TX, USA), following the manufacturer’s instructions. .. Root RNA extraction was performed following a similar protocol with pooled samples from five plants.

    Article Title: Role of Salicylic Acid and Components of the Phenylpropanoid Pathway in Basal and Cultivar-Related Resistance of Oilseed Rape (Brassica napus) to Verticillium longisporum
    Article Snippet: .. RNA Extraction and Synthesis of cDNA About 100 mg of homogenized fresh hypocotyl tissue was suspended in 1 ml TRI-reagent (Invitrogen™, ThermoFisher) and incubated for 15 min at room temperature. .. Subsequently, 100 µl bromochloropropane was added, and the samples were shaken for 15 s. After incubation for 15 min at room temperature, the mixture was centrifuged for 15 min at 10,159 rpm at 4 °C.

    Agarose Gel Electrophoresis:

    Article Title: Fluoxetine Inhibits Canonical Wnt Signaling to Impair Embryoid Body Morphogenesis: Potential Teratogenic Mechanisms of a Commonly Used Antidepressant
    Article Snippet: Total RNA was extracted from cell suspension prior to aggregation (day 0) and from EBs on days 1–4, using TRI reagent (LifeTechnologies) and Direct-zol RNA MiniPrep kit (Zymo Research, Irvine, California), and processed for cDNA synthesis using M-MLV Reverse Transcriptase (Promega, Madison, Wisconsin) and oligo-dT (18) primer. .. Standard reverse transcription and polymerase chain reaction (RT-PCR) was used to detect mRNAs of Slc6a4 (encoding the SERT) and Slc6a2 (encoding the norepinephrine transporter [NET]), using JumpStart REDTaq DNA Polymerase (Sigma-Aldrich, St Louis, Missouri) with the following conditions: initial denaturation at 94°C for 5 min; 35 cycles of 94°C for 15 s, 60°C for 20 s, and 72°C for 40 s; and a final extension at 72°C for 5 min. Amplified products were resolved in a 1% agarose gel and visualized by staining with ethidium bromide.

    In Vitro:

    Article Title: Crimean-Congo hemorrhagic fever virus nucleocapsid protein harbors distinct RNA-binding sites in the stalk and head domains
    Article Snippet: The DNA segment was gel purified and used as template in an in vitro T7 transcription reaction. .. The RNA was labeled with [α-32 P]CTP during synthesis and purified using Tri-Reagent (Ambion) followed by column purification using an miRNAeasy kit (Invitrogen), as previously reported ( ).

    Spectrophotometry:

    Article Title: Subtle sex differences in vasopressin mRNA expression in the embryonic mouse brain.
    Article Snippet: Arginine vasopressin (AVP) is a neuropeptide which acts centrally to modulate numerous social behaviors. .. Arginine vasopressin (AVP) is a neuropeptide which acts centrally to modulate numerous social behaviors.

    Concentration Assay:

    Article Title: Crimean-Congo hemorrhagic fever virus nucleocapsid protein harbors distinct RNA-binding sites in the stalk and head domains
    Article Snippet: The RNA was labeled with [α-32 P]CTP during synthesis and purified using Tri-Reagent (Ambion) followed by column purification using an miRNAeasy kit (Invitrogen), as previously reported ( ). .. All binding reactions were carried out in RNA-binding buffer (40 m m Tris-HCl, pH 7.4, 80 m m NaCl, 20 m m KCl, 1 m m DTT) at a constant concentration of RNA with increasing concentrations of the protein.

    CTG Assay:

    Article Title: Fluoxetine Inhibits Canonical Wnt Signaling to Impair Embryoid Body Morphogenesis: Potential Teratogenic Mechanisms of a Commonly Used Antidepressant
    Article Snippet: Total RNA was extracted from cell suspension prior to aggregation (day 0) and from EBs on days 1–4, using TRI reagent (LifeTechnologies) and Direct-zol RNA MiniPrep kit (Zymo Research, Irvine, California), and processed for cDNA synthesis using M-MLV Reverse Transcriptase (Promega, Madison, Wisconsin) and oligo-dT (18) primer. .. Note that primers for Slc6a4 (F: 5’-GTT CTG CAG CGA CGT GAA GGA AAT-3’, R: 5’- GCT TAG AGG GGA GGA GTC AAG GTG-3’) and Slc6a2 (F: 5’- GTG GTG GTC AGC ATC ATC AAC TTC-3’, R: 5’- AAC CAG CGT CAC GGA ATC ATT AGT-3’) were designed as intron-spanning, so that PCR products amplified from cDNA could be distinguished from those from genomic DNA based on the product size (416 and 520 bp for Slc6a4 and Slc6a2 mRNA, respectively).

    Staining:

    Article Title: Fluoxetine Inhibits Canonical Wnt Signaling to Impair Embryoid Body Morphogenesis: Potential Teratogenic Mechanisms of a Commonly Used Antidepressant
    Article Snippet: Total RNA was extracted from cell suspension prior to aggregation (day 0) and from EBs on days 1–4, using TRI reagent (LifeTechnologies) and Direct-zol RNA MiniPrep kit (Zymo Research, Irvine, California), and processed for cDNA synthesis using M-MLV Reverse Transcriptase (Promega, Madison, Wisconsin) and oligo-dT (18) primer. .. Standard reverse transcription and polymerase chain reaction (RT-PCR) was used to detect mRNAs of Slc6a4 (encoding the SERT) and Slc6a2 (encoding the norepinephrine transporter [NET]), using JumpStart REDTaq DNA Polymerase (Sigma-Aldrich, St Louis, Missouri) with the following conditions: initial denaturation at 94°C for 5 min; 35 cycles of 94°C for 15 s, 60°C for 20 s, and 72°C for 40 s; and a final extension at 72°C for 5 min. Amplified products were resolved in a 1% agarose gel and visualized by staining with ethidium bromide.

    Fluorescence In Situ Hybridization:

    Article Title: Teleost IgD+IgM− B Cells Mount Clonally Expanded and Mildly Mutated Intestinal IgD Responses in the Absence of Lymphoid Follicles
    Article Snippet: After 15 days, antibiotic-treated fish were sacrificed along with control fish maintained in the same conditions but not treated with antibiotics. .. The intestinal content was removed and the hindgut sampled and placed in TRI-Reagent (Thermo Fisher Scientific) to isolate total RNA following manufacturer's instructions.

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  • News
  • Press Release
  • Team
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  • 99
    Thermo Fisher tri reagent
    Tri Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tri reagent/product/Thermo Fisher
    Average 99 stars, based on 247 article reviews
    Price from $9.99 to $1999.99
    tri reagent - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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    Thermo Fisher tri reagent chloroform based rna purification
    Tri Reagent Chloroform Based Rna Purification, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tri reagent chloroform based rna purification/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tri reagent chloroform based rna purification - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

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